Biosaintifika: Journal of Biology & Biology Education
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Articles
1,068 Documents
<![CDATA[The Effect of Probiotic Lactobacillus paracasei on The Performance of Guppy Fish (Poecilia reticulata var. Mosaic) ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v15i1.39899
<![CDATA[Guppy fish (Poecilia reticulata var. Mosaic) is a type of ornamental fish that has many enthusiasts. Guppy fish culture still has low growth and viability in guppy fish culture. The previous solution was to apply antibiotics, but as time goes by antibiotics cause bacterial resistance. Another solution is the application of probiotics to feed. L.paracasei is one of the lactic acid probiotic bacteria and has never been applied to guppies. This study aimed to analyze the effect of the probiotic on performance (growth and viability, gill and gut histology, and LAB (Lactic Acid Bacteria L.paracasei) total) in guppies. 180 fish were randomly assigned into four triplicates groups and there were four treatment groups: 0 (Control), 5, 10, and 15 mL. kg-1 feed. As the result of this research, the highest body weight and length growth performance is found in the P3 group. Meanwhile, there were no significant differences between the groups of guppies viability. Gill and intestinal histology showed that control group was the best group. The conclusion of this study is that 15 mL. kg-1 is considered the most effective for improving the performance of guppy fish. The addition of L.paracasei can be used as feed supplements to improve performance of guppy fish.]]>
<![CDATA[Analysis of Genetic Variations of Ceiba pentandra (L.) Gaertn. on Several Critical Lands in West Sumatra Using RAPD Molecular Markers ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v15i1.40871
<![CDATA[Ceiba pentandra (L.) Gaertn. is a plant whose fruit is used to produce fiber and seeds for biofuel, which has the potential to be developed in critical land because it is adaptive to grow in critical land that is less fertile and lacks water. In West Sumatra, several critical land locations are overgrown by C. pentandra plants. This study aims to determine the genetic variation of C. pentandra in five critical land populations in West Sumatra as the basis for selecting superior seeds for development in critical land. The research was conducted by the descriptive method using molecular data with the molecular marker RAPD (random amplified polymorphic DNA). The results showed that the primers OPA-01, OPA-02, and OPB-10 could detect polymorphisms. Pangkalan Koto Baru in the regency Lima Puluh Kota (H = 0.1212) was the population with the highest intrapopulation genetic variation value. Interpopulation genetic variation (DST = 0.0321) was lower than intrapopulation genetic variation (HS = 0.1021), with a low genetic differentiation value (GST = 0.2392) and a high gene flow value (Nm = 1.5894). The genetic variation of C. pentandra can be used to select the parent in plant breeding programs.]]>
<![CDATA[In Vitro Mutagenesis of Dendrobium gabriella suryajaya Using Ethyl Methane Sulfonate (EMS) and Plantlet Regeneration ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v15i1.40997
<![CDATA[Plant breeding through mutation techniques has the main purpose to explore genetic diversity in the presence of useful traits for developing new plants. Ethyl Methane Sulfonate (EMS) is a widely used chemical to induce mutations in plants focused on obtaining genetic variation. EMS can induce random points of mutations and some of which can create new stop codons in the desired gene. EMS has been successfully used to generate morphological diversity and encourage the improvement of desired traits. The 3-month-old Dendrobium gabriella suryajaya Orchid protocorm-like bodies (PLB) treatment with EMS concentrations of 0.025%, 0.05%, and 0.075% can change genetic diversity, especially in leaf morphology. A total of 105 orchids were soaked in EMS solution at concentrations for 12 and 24 hours respectively. The phenotypic variations observed in this population include changes in leaf color and the number of buds. This EMS mutant population will be used for further studies including screening for various traits such as through ISSR analysis to determine the level of diversity. This research shows that mutagenesis using EMS can produce the amount of variability in Dendrobium. The generation of variability for desired traits resulted in the identification of several mutants with important agronomic characteristics that can be used as germplasm for improvement.]]>
<![CDATA[Enhancement of Microalgal Metabolite Production through Euglena sp. Local Strain and Glagah Strain Consortia ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v15i1.41895
<![CDATA[Euglena sp. is green microalgae in an acidic environment (pH 2.5-3.5). Euglena sp. has recently been developed widely in industry because of its capability to produce lipids that can be utilized to synthesize biofuel. Microalgae is a potential source of biodiesel, especially in the form of a consortium culture. One of the microalgae consortium cultures that have been explored is the nature consortium microalgae of Glagah strain. The Glagah consortia were isolated from Lagoon in the Glagah Beach, Kulonprogo, Yogyakarta. This study aimed to determine the total production of biomass, lipids, carbohydrates, and proteins of mixed culture of Glagah strain consortium and Euglena sp. as biodiesel substrate. The biomass test was measured using the dry weigh method using a filtration vacuum pump kit, lipids were measured using the Blight Dryer method by adding chloroform and methanol as solvents, carbohydrates were measured using the Sulfur Phenol Acid method by adding Phenol and sulfuric acid (H2SO4), and proteins were measured using the Bradford method by adding SDS and Bradford’s solution. The total production and productivity of biomass, lipids, carbohydrates, and proteins showed that the mixed culture of Glagah strain consortium with Euglena sp. was higher than the Glagah strain consortium. It reached 0.410 g/L; 0.253; 0.856 g/L; and 0.623 g/L. Therefore, it could be concluded that the mixed culture of the Glagah strain consortium with Euglena sp. could increase the production of biomass, lipids, carbohydrates, and protein up to two times that of the Glagah strain consortium so that this mixed culture treatment could be used as a reference in microalgae cultivation for biodiesel.]]>
<![CDATA[Plant Regeneration in Amorphophallus muelleri Blume. through Organogenic ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v15i1.40501
<![CDATA[Porang (Amorphophallus muelleri Blume.) usually used to functional food raw materials, caused its high glucomant content. Limitation of conventional propagation both vegetative and generative affected to decreasing of porang production to fulfil market demand. To solve the problem, plant tissue culture technique was chosen to rapid propagate bulbils of porang. So, the aim of this research to determine the combination and concentration of Plant growth Regulators (PGRs), such as 6-Benzylaminopurine (BAP), Naphthaleneacetic Acid (NAA), and Thidiazuron (TDZ) for quality of callus, also growth of callus and shoots for rapid propagation of porang, cause PGRs are one of factors that affect the success of in vitro propagation. Experimental design of this research was completely randomized designed with statistical analysis using analysis of variance (Anova) one way. Difference combination and concentration of cytokinin and auxin as manipulation variables, which several parameters growth of callus, shoots, and roots was observed to indicate the rapid organogenic of porang. The optimal shoot induction medium was Murashige and Skoog (MS) media with 5.0 mg.L-1 BAP and 0.2 mg.L-1 NAA on which number of shoots and shoot height reached 15±1.8 and (7.2±1.86) cm. After shoot formation stage, the proper media for rooting stage was determined, which was Murashige and Skoog (MS) media with 1.0 mg.L-1 NAA resulted the rooting rate reached 100% and roots appear earlier. Optimal combination and concentration of PGRs on micropropagation of Amorphophallus muelleri Blume. from bulbils explant should be known to increase the production of porang, which can fulfil the market demand.]]>
<![CDATA[Antifungal Potential of Cell-Free Supernatant Produced by Keratinolytic Fungi against Ganoderma boninense ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v15i1.38325
<![CDATA[Keratinolytic is a microorganism ability to degrade keratin substrates. Fungi are known to produce the enzyme keratinase. This research was carried out aiming to know the keratinolytic fungi potential in inhibiting Ganoderma boninense fungi. Five isolates of keratinolytic fungi coded A 31, A 18, A 12, K 18, and A 29 have antagonistic ability against G. boninense. A 12 and A 18 had the largest inhibitory diameter of 25.23 mm and 24 mm, respectively, after 7 days of incubation. The ability of A 12 and A 18 antagonisms was observed by testing the supernatant of keratinolytic fungi incubated in a feather meal broth medium. An 18 has the largest antagonism percentage of inhibition at 84.72% against G. boninense, followed by A12 (77.78%) and combination treatment of A 12 + A 18 (73.55%) after 7 days of incubation. Keratinolytic fungi can also grow on minimum salt chitin medium (MSCM) and produced a hydrolysis zone after 3 days of incubation. G. boninense hyphae abnormalities were observed by using a 100x magnification microscope. Moreover, hyphae lysis and dwarf, curved, rolled, and curly hyphae were also observed after giving treatment on A 12, A 18, and the combination treatment of both isolates. These results showed that both keratinolytic fungi isolates and the supernatant are powerful biocontrol agents against G. boninense. There is no previous research report on endophytic fungi inhibiting the growth of Ganoderma boninense. In the future, keratinolytic fungi can be applied in agriculture technology. ]]>
<![CDATA[Transaminase Enzyme and Liver Histopatological Structure of Mice Facing to Smoke Cigarettes After Administerred with Enhalus acoroides Peel Extract ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v15i1.40725
<![CDATA[The objective of this research were aimed to determine the level of transaminase enzymes and the histopathological structure of mice liver exposed to cigarette smoke after treatment of E. acoroides peel extract. Completely randomized design was conducted with 3 doses treatment groups (P1, P2, P3) and a control group (K+, K-) with 3 times repeated. The test was carried out during 14 days for cigarette smoke, and 14 days for seagrass peel extract tretment. The data measured were the levels of SGOT, SGPT and were analyzed using the Anova test. Liver histopathology (necrosis, parenchymatous, hydropic degeneration and normal) were described descriptively. The results showed that the administration of seagrass E. acoroides peel extract had a significant effect on decreasing levels of SGOT, SGPT (p value 0.05) when compared with K+. The P3 treatment had lower levels of SGOT (80.07±3.1 IU/L) and SGPT (88.3±2.1 IU/L). Liver histopathology consist of 34 necrosis cells less than the positive control group (K+) and normal cells were 47, higher than the positive control group (K+). It can be concluded that E. acoroides peel extract had a significant effect on SGOT, SGPT levels in mice exposed to cigarette smoke. Peel extract of E. acoroides 75 mg/kgBW (P3) was an effective dose to reduce the levels of SGOT, SGPT and improve the histopathological structure of mice liver exposed to cigarette smoke. This research is expected to be useful for the community, especially in the coastal areas regarding the benefits of using E. acoroides as a therapeutic agent against free radicals originating from cigarette smoke.]]>
<![CDATA[Clitoria ternatea L. Extract as Adjuvant Therapy on Reducing IL–6 Levels in Reversal Reaction ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v15i1.39808
<![CDATA[Reversal reaction is a type IV cellular hypersensitivity reaction in leprosy. Interleukin-6 is a mediator with a pleiotropic effect on inflammation and immune response. Steroids are the standard therapy in the treatment of reaction reversal. Clitoria ternatea flower extract has anti-inflammatory properties. Long-term use of steroids can cause side effects. Therefore, it is necessary to give adjuvant therapy that can shorten the treatment period. This study aims to analyze the effect of adjuvant therapy of Clitoria ternatea extract to reduce IL-6 levels in reversal reaction. Experimental research design with pre and post-randomized single-blinded controlled trial, involving 22 subjects with reversal reaction. The control group received standard therapy and the treatment group received standard and adjuvant therapy with Clitoria ternatea extract. IL-6 levels were measured by ELISA. Data analysis used the Wilcoxon test and Independent T-test. The results showed a significant difference in treatment group (p=0.003) and control group (p=0.016). The mean decrease in serum IL-6 levels in treatment group was 81.35 pg/ml and in control group was 24.30 pg/ml (p=0.027). Clitoria ternatea showed a significant IL-6-lowering effect in patients with reversal reactions. This study has demonstrated the potential of Clitoria ternatea extract as adjuvant therapy in patients with leprosy reactions.]]>
<![CDATA[Development and Validation Model of Peatland Conservation through Interdisciplinary Science Learning ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v15i1.43846
<![CDATA[The peatland conservation model is generally developed based on the community. A new education-based conservation model was developed through temporary activities through seminars, workshops or non-formal learning. The aim of this research is to develop and validation a peatland conservation model through interdisciplinary science learning that connects universities, schools and communities in peatland conservation activities. The development of a peatland conservation model went through third stages following interdisciplinary research patterns. Validation was determined by content and empirical validation. The peatland conservation model consisted of three interrelated dimensions in peatland conservation, namely universities, schools and communities. The model was equipped with implementation tools in the field in the form of science learning tools. The form of a peatland conservation model book, teaching practice materials, an environmental literacy questionnaire, and an interdisciplinary thinking performance rubric were developed in this study. The validated aspects included theory, assumptions, and conceptual model representations with the Aiken score was 0.88 as a valid category and the ICC value of 0.457 showed that the reliability was not good. Empirical validation showed that the dimensions of the university, school and community constructs had internal consistency reliability and validity. The relation between dimension that universities had an influence on schools and society, and schools have an influence on society. The peatland conservation model was equipped with practical teaching learning tools that directly connected universities and schools. However, this model was also suitable for non-educational natural sciences courses or pure natural sciences to form interdisciplinary thinking skills and environmental literacy.]]>
<![CDATA[Development of Pteridophyte Taxonomic Learning Resource to Foster Cognitive and Psychomotor Learning Outcomes ]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 14, No 3 (2022): Desember 2022 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v14i3.42919
<![CDATA[Authentic learning resources are meaningful for Biology learning, particularly in conceiving Plantae topic. The availability of plants, especially Pteridophyte in school environment is very limited and require more adequate plant learning resources. This study aimed to develop a Pteridophytes Taxonomic Learning Resource (PTLR) and tested its validity, practicality, and effectiveness. Research and Development were done refers to the ADDIE model. The PTLR was validated by a taxonomist and instructional media expert. The practicality was observed from the responses of teachers and students, while the effectiveness was explored based on cognitive and psychomotor learning outcomes. The PTLR was developed by container gardening method with 29 fern species representing the classes of Pteridophyte and showed specific characteristics which organized in their taxonomic groups. The results showed that PTLR is very valid with average score of 85.5% and considered practical by teachers (81.25%) and students (90.65%). The PTLR was also effective in achieving two basic competencies by Curriculum of 2013 of Biology with cognitive outcome N-gain of 0.57 (moderate) and psychomotor 86.34% (very good). In summing up, the PTLR is recommended as a learning resource of Plantae topic at senior high school. This research result enriches the types of innovative biology learning resources.]]>
