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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 100   101   102   103   104 
Utilization Of Plant as A Drug for Diabetes Mellitus By The Community Of Beutong District, Nagan Raya Regency Icha Putrimarlin; Hasanuddin Hasanuddin; Safrida Safrida; Wardiah Wardiah; Dewi Andayani
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.36480

Abstract

Diabetes mellitus is one of the leading causes of death in the world. Indonesia is a country with the 7th largest population of Diabetes mellitus sufferers in the world in 2015. Many people use plants as diabetes mellitus drugs, in Indonesia More than 500 types of plants have been shown to have hypoglycemic activity because these plants contain compounds that are efficacious as anti-diabetic. This study was aimed to determine as many types of plants as possible that are used as medicinal plants for diabetes mellitus in Beutong sub-district, Nagan Raya district. This research was conducted by conducting interviews with 100 respondents aged 18-60 years who knew information about plants used for Diabetes mellitus.This study used the method of interview, observation, and identification (PlantNet). The results found as many as 29 species of plants from 26 families. Myrtaceae family as the most numerous plants with the number of species as much as 10% and the family Fabaceae with the number of species as much as 7%. Leaves are the part that is most widely used as medicine and the majority of people process it in a different way. Consume by drinking more than by chewing and the recommended dose for consumption is 2 times a day after meals. Benefits for researchers can add information about medicinal plants for diabetes mellitus utilized by the people of Beutong District, Nagan Raya Regency and become one of the references for public knowledge traditional, and become a reference material for students in conducting research next.
Properties of Bio-Silver Nanoparticles Mediated by Tuber and Leaf Extracts of Manihot esculenta Synodalia C. Wattimena; Desy A. Ayuningrum; Leonita Y. Latuasan; Efraim Samson; Philipus J. Patty
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.37667

Abstract

Bio-silver nanoparticle using plant extract has been the subject of many studies nowadays. Researchers use various plant extracts, especially the popular plant from their places. This study aims to synthesize AgNPs using leaf and tuber extracts of M. esculenta Crantz and to characterize their properties to be compared one to another. The characterization includes surface plasmon resonance wavelength using UV-VIS spectroscopy, the chemical bonds related to the extract on the surface of the particles using FTIR spectroscopy, shape and size of the particles using TEM, and antibacterial properties using the disc diffusion method. Each tuber and leaf extract AgNPs were formed a few minutes after mixing silver nitrate with each extract indicated by the change of the color from transparent to yellowish-brown. The color of the sample was quantified by the wavelength of surface plasmon resonance which was found to be 425 nm for tuber extract AgNPs and 430 nm for leaf extract AgNPs. The results of FTIR spectroscopy indicate the presence of the extract at the surface of nanoparticles for both samples. The particles are mostly spherical, but the diameters of the leaf extract AgNPs are relatively smaller than those of the tuber extract AgNPs. The results of antibacterial assays of both samples show that both AgNPs inhibit the growth of S. aureus as effectively as they inhibit the growth of E. coli.
DNA Barcode of Red Junglefowl Gallus gallus L, 1958 (Aves: Phasianidae) of Sumatra Based on Mitochondrial COI DNA Gene Jarulis Jarulis; Nurmeiliasari Nurmeiliasari; Hery Haryanto; Iqwati Vilanda
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.36530

Abstract

Genetic data of red junglefowl (Gallus gallus) from southern Sumatra is valuable for conservation efforts in Indonesia. A mitochondrial COI DNA gene sequencing was performed to elucidate its genetic character, single nucleotide polymorphism, genetic distance, and phylogeny. Blood samples (±0.5 ml) from 20 individuals of Gallus gallus were taken from the living collections of the people of Bengkulu Province (Central Bengkulu Regency and Seluma Regency) and South Sumatra Province (North Musi Rawas Regency) from May to November 2021. Total DNA isolation followed the procedure of The Spin-Column Protocol Kit uses the Dneasy® Blood and Tissue Kit, Qiagen. DNA replication using the Polymerase Chain Reaction technique with primers (COIGG_F and COIGG_R) using MEGA 10.0 software and Bioedit for data analysis. The results revealed716 conserved sites, 16 variable sites, 9 parsimony sites, and 6 singleton sites from the 732 bp nucleotide sequence. Six specific sites (SNPs) as barcodes for Sumatran Junglefowl were found at sequences 51, 273, 327, 721, 729, and 732. The mean genetic distance between individuals was 0.1%, between populations was 0.8%, between species was 7.4%, and between genera was 15.5%. The red junglefowlof South Sumatra Province and Bengkulu Province are closely related with 98% bootstrapping and separated from other Gallus in the same group (ingroup) with 100% bootstrap. The Gallus-gallus group is quite far apart from the outgroup species in the Phasianidae family with 47-100% bootstrap. Red junglefowl from southern Sumatra has genetic differences from other chickens in the world and these differences can be used as a species barcode and as origin identification the widely traded red jungle fowl.
Multiple Tolerances of Cassava Germplasm to Drought Stress and Red Spider Mite Attacks Herdina Pratiwi; Tinuk Sri Wahyuni; Novita Nugrahaeni
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.35781

Abstract

Uncertain climate change encourages the assembly of cassava varieties with multiple tolerances to both abiotic and biotic stress. The research aimed to evaluate the multiple tolerances of cassava germplasm to drought stress and red spider mite attacks. The research was held at Installation for Research and Assessment of Agricultural Technology of Muneng from February to December 2019 using a randomized block design nested in two environments with two replicates. The treatments consisted of 50 cassava accessions from the Indonesian Legumes and Tuber Crops Research Institute collection and two irrigation environments, i.e. a normal environment and a drought environment. Drought stress caused a decrease in plant height, tuber yield; on the other hand, increased red spider mite attack. Eight accessions have Stress Tolerance Index values reaching above 1.00, and two of them also have resistance to red spider mites.  Accessions MLG 10361 and MLG 10362 had a high level of tolerance to drought stress as well as resistance to red spider mites so that both accessions may be used as a source of multiple resistance genes for biotic and abiotic stresses. 
Inulinase Producing Yeast Isolated from Kepok Banana Peels (Musa acuminata x balbisiana) Romario Dion; Wijanarka Wijanarka; Sri Pujiyanto
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.37117

Abstract

Inulinase enzyme (EC 3.2.1.80) is an enzyme capable of hydrolyzing inulin and is used as a catalyst in the production of High Fructose Syrup (HFS) and fructooligosaccharides (FOS). Inulinase enzymes can be produced by yeasts found in waste such as kepok banana peels (Musa acuminata x balbisiana). The aims of this study were to obtain the inulinase enzyme-producing yeast from kepok banana peels and to determine the effect of commercial inulin concentration and incubation time on enzyme activity. This research was conducted experimentally using a factorial completely randomized design with factors being inulin concentration of 1% (K1), 3% (K2) and 5% (K3) and incubation time of 6th (W1), 12th (W2), 18th (W3), and 24th hours (W4). The results showed that there were 4 isolates of inulinolytic yeasts obtained from kepok banana peels, namely YP1, YP2, YP4, and YP6. However, the most potential isolate to be used in inulinase production was YP2 isolate because it had the highest inulinolytic ratio value of 1.96. The results of inulinase activity showed that only inulin substrate with concentration of 5% (K3) had a significant or significant effect on inulinase production.
The Role of Bacteria in the Termites Intestine Macrotermes gilvus Hagen as a Biological Agent in the Degradation of Medical Mask Waste Niken Subekti; Retno Wulandari
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.37711

Abstract

The use of disposable medical masks during the Covid-19 pandemic can cause solid waste problems in the environment. The subterranean termite Macrotermes gilvus  Hagen has the potential to degrade medical mask waste due to the presence of microorganisms in its intestines. The purpose of this study was to analyze the effect of adding starter bacteria from the intestine of the subterranean termite M. gilvus  Hagen and the most optimal composting time in the degradation of medical mask waste according to SNI standards based on physical properties (color, smell, and texture), chemical properties (C-Organic content, N content, and C/N) compost. The results showed that the addition of bacterial starter from the subterranean termite intestine M. gilvus  Hagen with a concentration of 50% and a composting time of 5 weeks gave the best compost yield according to SNI No. 19-7030-2004 because it has a dark brown color, crumb texture, smells like soil, 20.22%  C-Organic content, 1.35% total N content, and 15.14% C/N content. This research can be recommended as an alternative solution for waste management using biological agents.
Biodegradation Test of Polluted River Caused by Domestic Wastewater Using Indigenous Bacteria in the Way Tomu Watershed, Ambon City
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.40016

Abstract

Nowadays, the rivers are often used by residents around the river as the final disposal of domestic wastewater that causes the rivers to become polluted. Biodegradation is an alternative to wastewater treatment as an effort to solve water pollution which is often carried out using bacteria that have the potential to decomposers in the biodegradation process, especially indigenous bacteria. This study aims to identify and obtain isolates, combinations, and characteristics of the indigenous bacteria isolate that have the potential as a biodegradation agent for polluted river caused by domestic wastewater in the Way Tomu watershed, Ambon City. In this study, isolates of indigenous bacteria that were isolated and purified were tested for protease and amylase enzyme activity to select isolates to be used in the biodegradation process testing consisting of parameters pH, COD, and TSS as indicators of the biodegradation. Afterward, the isolates that have the best potential as biodegradation agents will be characterized and biochemical tests will be carried out to identify the general characteristics of these isolates. The results showed that the combination of Genus Aeromonas, Bacillus, and Pseudomonas as isolates of indigenous bacteria that has the best potential as an agent for the biodegradation of polluted river caused by domestic wastewater. The benefit of this research is as initial information and consideration for alternative treatment of polluted rivers caused by domestic wastewater using indigenous bacteria isolates as an effort to resolve river pollution, especially the rivers in Ambon City.
Reproductive Biology of Greenback Mullet, Planiliza subviridis (Valenciennes, 1836) from Segara Anakan Cilacap
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.39696

Abstract

Greenback Mullets (Planiliza subviridis) are a commercial commodity and are fished daily in the Segara Anakan Cilacap, although has not been overfished. Continuous and unregulated fishing might harm the Greenback Mullets population. Therefore, sustainable fisheries of this species are still needed. Reproductive biology is among the essential aspect that supports sustainable fisheries. This research aims to obtain information on the reproductive biology of Greenback Mullet in Segara Anakan Cilacap. The research used a survey method with a purposive random sampling technique. The observed parameters were the gonad maturity stage, gonad maturity index, and fecundity. The data were analyzed descriptively. The female individuals have gonad maturity stages III and IV, gonad maturity stage was 16.81±2.59 and 16.92±2.51, and fecundity was 37,208-183,661 and 38,468 - 272,835 in August and October, respectively. Conversely, male individuals have a low gonad maturity stage and gonad maturity index. Based on the data, Greenback Mullets in the Segara Anakan estuary have maximum gonad maturity stage, gonad maturity index, and fecundity in August and October, while in February they were in early gonadal development. These data are essential for sustainable fisheries management in the Segara Anakan estuary Cilacap, Central Java, especially for Planiliza subviridis.
Bioremediation of Cadmium (Cd) in Batik Wastewater Using Different Carrier Media Containing Rhizobacteria Lestari, Sri; Oedjijono, Oedjijono; Santoso, Slamet; Kusharyati, Dyah Fitri; Fathurrohim, Ramadi Habib
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.37683

Abstract

Batik wastewater is very dangerous for the environment and health because it contains heavy metals, such as Cadmium (Cd), derived from naphthol and indigo sol dyes. Rhizobacteria have great potential to detoxify Cd on a laboratory scale. Therefore, they should be packaged in carrier media to ensure their long-term viability in the field. The carrier media used were peat, sawdust, and tofu solid waste. This research aimed to determine the most potential of three rhizobacteria as tolerant to Cd from 11 isolates, the best carrier media that can maintain rhizobacteria viability after freeze-dried, and the effectiveness of carrier media containing potential rhizobacteria in reducing Cd in batik wastewater. Furthermore, an experimental method with completely randomized and split-plot designs was used. Rb1, Rb3, and Rb6 were the most Cd-tolerant of the three rhizobacteria isolates tested. For the viability of each isolate, tofu solid waste and peat were the best carrier media at Rb, while sawdust and tofu solid waste were the best at Rb3 and peat at Rb6. Isolate Rb3C3 had the highest percentage value of degrading Cd at 85.1%, while others had less than 80%. Isolate rhizobacteria with a carrier media makes it easy to apply on a field scale because it already contains a source of nutrients for bacterial growth and the packaging has a longer shelf life.
Taxonomic Confirmation of Rare Fruits in West Kalimantan Using rbcL and matK Sequences
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.40284

Abstract

Phylogenetic analysis for plants can be very helpful in determining species identification or taxonomic status morphologically. rbcL and matK are widely used as genetic markers in constructing seed plant phylogenies. Different identification and new collection during the re-inventory from the previous study were found in two and four types of rare fruits in Sibohe Forest, West Kalimantan. The six types include pisang karok (Musa sp.) and Kandis (Garcinia sp.), as well as nubik (Artocarpus sp.), tehengan (Artocarpus sp.), smallest arok (Ficus sp.), and amok (Alpinia sp.). This study aimed to analyze the phenetic relationship of six rare fruits from Kalimantan based on rbcL and matK genes. DNA samples were obtained from dried body parts of the previous study and amplified by PCR using both forward and reverse primers for rbcL and matK genes. Amplification was observed on electrophoresis gel for rbcL gene of nubik, tehengan, smallest arok, amok, and pisang karok, while only pisang karok in matK gene. However, matK gene sequence was also obtained for amok even though the band was not seen on the gel. Phylogenetic analysis using the two genes confirmed the morphological identification reported in the previous study; however, using matK as a single gene for taxonomic confirmation must be reconsidered. The information of rbcL and matK sequences of six rare fruits from West Kalimantan could be the first information for building the DNA barcodes of the rare fruits in West Kalimantan.

Page 102 of 107 | Total Record : 1068

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