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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 103   104   105   106   107 
AGRONOMIC PERFORMANCE OF SESAME (Sesamum indicum) UNDER DIFFERENT FERTILIZER MANAGEMENT Dollison, Mohammad D.; Dollison, Beverly Branzuela
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.47277

Abstract

The sesame crop is one of the earliest crops that is being grown for edible oil purposes. The production performance of this crop is often affected by different factors such as soil fertility, water, and climatic conditions. This study evaluated the performance of sesame under different fertilizer management like animal manure, guano, vermicast, inorganic fertilizer, and no fertilizer application as check plots. The study showed that applying guano fertilizer significantly improved plant height performance 30 to 60 days after planting compared to inorganic fertilizer, other organic fertilizers, and treatments without fertilizer application. The number of capsules per plant produced by sesame was significantly higher with guano fertilizer application compared to treatments without fertilizer application and other organic and inorganic fertilizer sources as treatments. Similarly, the yield performance of sesame under different fertilizer applications resulted in a significant performance over guano fertilizer, with the highest yield obtained compared to other fertilizer management. The different organic and inorganic fertilizers are good sources of essential nutrients for sesame production. Likewise, further study can be conducted using a fertilizer combination, both organic and inorganic, for a better fertilizer management recommendation.
Hatching Failure and Success of Hawksbill Sea Turtles (Eretmochelys imbricata) in Semi-Natural Nests Karimunjawa. Rahayu, Sari Sistyawati; Suryanti, Suryanti; Ayuningrum, Diah; Lundin, Kennet
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.48658

Abstract

Hatching success and failure is influences by the interaction between biotic and abiotic factors during the incubation period. Non optimal nest is susceptible to bacterial contaminated eggs during incubation period and hatchling morphology. The purpose of the study is to understand the relationship between environmental conditions of semi natural nest with bacteria contaminant, hatchlings allometry and abnormality. The method used was quantitative descriptive which are nest temperature and humidity measurement, hatchlings allometry measurement, bacteria observation of gram negative with EMB (Eosin Methylene Blue) agar, TPC (Total Plate Count), bacteria characteristic morphology and hatchlings abnormality observation. The result showed that semi natural nest have high temperature (30˚C) and high humidity (70%) caused smaller hatchlings and negative allometry growth, hatchlings abnormality and bacterial contaminant. TPC showed that the number of bacteria unhatched eggs (1,42 x ) more than hatched eggs (1,03 x ). The bacteria colonies were suspected to be from Enterobacteriaceae family according to microscopic and macroscopic morphological characteristic. Macroscopically, the bacteria morphology is dominated by a round shape, concentric surface, flat edges, flat elevation and pink color, meanwhile microscopic form is bacilli. There is significant correlation between environmental conditions of semi natural nest with hatchlings growth, hatchlings abnormality and eggshells bacteria
Effect of Ascorbic Acid, Activated Charcoal and Dark Incubation on Browning Intensity of Saurauia bracteosa In Vitro Culture Putri Octaviana Ginting, Naomi Bastanta; Restiani, Ratih; Prasetyaningsih, Aniek; Meyga Semarayani, Cokorda Istri
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.48439

Abstract

Saurauia bracteosa, also known as Pirdot, is an endemic woody plant of the Actinidiaceae family with medicinal properties. Its population decline in nature has led to a vulnerable status, prompting conservation efforts, such as in vitro culture. Browning poses a significant challenge in the woody plant in vitro culture. Browning inhibitors such as ascorbic acid, activated charcoal and dark incubation can be used to address this issue. This study aims to determine the effect of ascorbic acid, activated charcoal and incubation conditions on browning inhibition of S.bracteosa. The study employed a Completely Randomized Design with 12 treatments on leaf explants, repeated five times. Observations, including the time of browning formation, percentage of browning, intensity of browning and percentage of live explant were carried out for 30 days and analyzed using ANOVA followed by DMRT. The results showed that the addition of activated charcoal in MS media with dark incubation delayed the browning formation time (12.40 DAI) while the adding ascorbic acid in MS media and dark incubation resulted in the lowest browning intensity (0.22). These research findings can serve as a foundational protocol for browning prevention, supporting the successful in vitro conservation of S. bracteosa.
Isolation and Molecular Screening of Fungus as Agents in Cellulolytic Transformation Materials from Symbiotic Lichen Rahardiyan, Dino; Moko, Emma Mauren
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.44969

Abstract

 The abundance of lignocellulosic waste makes it a potential source for advanced biomaterials through various transformation processes. Lignocellulosic biomass transformation to advanced biomaterials involves enzymes from extracellular metabolites of microorganisms capable of hydrolyzing lignocellulose. This research was to molecularly screen fungi found in symbiotes of lichen endemic to trees growing in North Minahasa (North Sulawesi) with lignocellulolytic transformation enzymes. Molecular screening was conducted from identified fungi isolates based on partial genetical analysis on the locus of Internal Transcribed Spacer (ITS) of the fungi’s ribosomal DNA. Fungi isolates screening identified Trichoderma koningiopsis (isolate HZA8 and isolate HZA6), Penicillium sumatraense (strain CBS 127365 and strain CBS 130380), Trichoderma hamatum (isolate PAN12-45 and isolate PAN12-05), Aspergillus aculeatus (strain A1.9 18S),  Aspergillus aculeatus (isolate XSD-74), Trichoderma reesei (strain S2606 and isolate 5A14).  Molecular identification and BLAST homology of potentially lignocellulolytic fungi isolates rDNA indicated that isolate KB2 had close relationship with Trichoderma reesei at 100% degree of closeness and an index of cellulolytic activity of 1.19. While isolate KB3 appeared closely related to Aspergillus aculeatus at 99.83% degree of closeness and an index of cellulolytic activity of 1.57. Therefore, the potential of developing bioprocess industries in general and in particular is most probable.
The application of Rigidoporus sp J12 and Stenotrophomonas maltophilia BM in the degradation of batik waste Subowo, Yohanes; Suliasih, Suliasih; Widawati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.41987

Abstract

The batik industry in Indonesia produces batik waste which pollutes the environment. This waste can be degraded using laccase-producing microorganisms. The microorganisms used in the research were the fungus Rigidoporus sp J12 and the bacteria Stenotrophomonas maltophilia BM. This research aims to determine the ability of Rigidoporus sp J12 and Stenotrophomonas maltophilia BM and their consortium in producing laccase, observing their ability to degrade Poly R-478 which is an indicator of phenoloxidase activity and batik waste. Microorganisms are grown in growth media and then placed in media containing Poly R-478 or batik waste. Inducers are added to increase laccase activity. The inducers used were 15 g/L sucrose, 200 µM CuSO4 and 40 mM veratryl alcohol. The results showed that Rigidoporus sp J12 and Stenotrophomonas maltophilia BM produced laccase in PDB and NA media. The highest laccase activity was found in the enzyme produced by Rigidoporus sp J12 in PDB media at a temperature of 40°C, media pH 6.0 and the addition of sucrose. Rigidoporus sp J12 degraded batik waste by 39.38% and increased by 2.12 times after adding sucrose and incubation for 15 days. These bacteria and fungi can be used to degrade batik waste in order to prevent environmental pollution. Using the fungus Rigidoporus sp J12 purely is more profitable than using it with S. maltophilia BM bacteria.
Antibacteria Activity Peel and Seed Extracts of Rambutan (Nephelium lappaceum L.) Against MDR Bacteria Causing Urinary Tract Infections Mukaromah, Ana Hidayati; Cahyaningrum, Dea Galuh; Putri, Desty Ratna; Jannah, Erni M; Rinaldi, Muhamad Rizqi; Wardoyo, Fandhi Adi; Ariyadi, Tulus; Hikmah, Annisa Nurul; Darmawati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.39568

Abstract

Multidrug-resistant (MDR) - Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) are the main causes and have become serious problems in urinary tract infections, so antibacterial agents derived from biological materials are needed. ESBL-E. coli and ESBL-K. pneumoniae bacteria are resistant to extracts from rambutan peels and seeds, but there is no knowledge of the use of different solvents, such as n-hexane, chloroform, or ethanol. The objective of this research was to assess the antibacterial activity of rambutan peels and seed extracts (n-hexane, chloroform, and ethanol) against MDR bacteria that cause urinary tract infections (UTI). The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values for antibacterial activity were calculated using agar well diffusion and dilution procedures. The results demonstrated that the ethanol extract of rambutan peels had inhibitory zones against MDR K. pneumoniae and E. coli that varied from 9.00 to 14.13 mm. 15.625 mg/mL For MDR E. coli and 3.90 mg/mL for MDR K. pneumoniae, respectively, the MIC value was determined. The MBC value was 62.50 mg/mL for MDR K. pneumoniae and 31.25 mg/mL for MDR E. coli. Conclusion: Of the six rambutan peel and seed extracts, the ethanol extract has greater potential as an antibacterial agent. It is advised that more in-vivo studies be done to understand how the antibacterial activity operates.The benefits of research for the science are providing alternative solutions to antibiotic resistance, to further advancing the field of antimicrobial research, and reducing the risk of bacterial infections.
Application of Rabbit Urine and Manure Based Fertilizer on the Growth of Arabica and Robusta Coffee Seedlings Prajanti, Sucihatiningsih Dian Wisika; Litaay, Christina; Widiatningrum, Talitha; Amelia, Dhea Rizky; Daud, Dalila
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.48027

Abstract

Coffee farming demands substantial amounts of fertilizer. Furthermore, the expensive cost of chemical fertilizers prompt coffee growers to seek alternative remedies. One emerging solution is the utilization of organic fertilizer. This study aims to assess the impact of organic fertilizer, specifically made from rabbit manure and urine, on the growth of Arabica and Robusta coffee seedlings. The experiment focused on the context of coffee cultivation in Temanggung Regency, comparing the effects with goat manure fertilizer. The method was initiated by producing organic fertilizer, encompassing rabbit manure, goat manure, and rabbit urine. Then, the fertilizer was applied to Arabica and Robusta seedlings. The research employs quantitative and descriptive analyses. Quantitative data encompasses the nutrient content of each fertilizer and soil, plant height, leaves number, and stem diameter. Descriptive analysis involves the overall performance evaluation. The application of rabbit manure and urine as fertilizers to Arabica and Robusta coffee seedlings yields positive outcomes, i.e. enhancing plant height, leaves number, and seedling diameter. Among the tested organic fertilizers, rabbit urine notably influenced the plant height, leaves number, and stem diameter of both seedlings. The performance of the rabbit urine treatment exhibited healthier attributes compared to those without any fertilizer, such as sturdy leaves and robust stems. Additionally, the root systems of the untreated plants appear less vigorous with sparser root hairs.
Isolation and Molecular Identification of Proteolytic Bacteria from Rusip an Indonesian Fermented Food Amatullah, Lutfiana Hary; Afifah, Diana Nur; Jannah, Siti Nur
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.42237

Abstract

Processing fish with the fermentation method can increase the nutritional content, bring out a distinctive taste, and make the fish last longer. Rusip is a type of complementary food from Bangka Belitung in the form of fish sauce that has been rotten or fermented. Rusip is made from fermented anchovy (Clupeoides borneensis). The concept of fermentation in Rusip is the breakdown of complex protein molecules by proteolytic bacteria into simple amino acid components.The purpose of this study was to determine the presence of proteolytic bacteria that play a role in rusip fermentation.The media used in screening proteolytic bacteria is skim milk agar (SMA) media. Then it was incubated at 37oCfor 24 hours. Bacterial colonies that form a clear zone on the media indicate that these bacteria can degrade protein. After the isolation process and isolates were obtained, the bacteria were characterized by Gram staining method and molecular identification.The results showed that there were proteolytic bacteria in Rusip food, and the 3 best isolates that produced protease enzymes were identified as Bacillus cereus.

Page 107 of 107 | Total Record : 1068

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