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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 92   93   94   95   96 
Seedling Production of Pak Choy (Brassica rapa L.) using Organic and Inorganic Nutrients Dody Priadi; Fiqolbi Nuro
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8537

Abstract

Pak Choy or Bok Choy (Brassica rapa L. var. chinensis) is one of favorite Chinese leafy vegetable for various dishes in Indonesia. In this study, it was used as a plant model to identify the appropriate organic hydroponic nutrient solution for leafy vegetable seedling production. The seed was sown on rock wool slabs submerged with 200 ml of a nutrient solution containing biofertilizer of Beyonic StarTmik@Lob (0, 25, 50, 75, and 100%). commercial hydroponic solution (0, 25, 50, 75, and 100%) and its combination (25, 50, and 75%). The experiments were arranged in a CRD. Meanwhile, the obtained data was analyzed using ANOVA followed by DMRT. The relationship among growth parameters was observed using Pearson correlation analysis.  The result of the study showed that the combination of organic and inorganic nutrient (25% Beyonic StarTmik@Lob and 75% commercial hydroponic solution) resulted in the highest seedling growth parameters and leaf indices as well as the perfectly positive correlations among growth parameters. This result indicated that the use of organic nutrient alone was not appropriate for hydroponic seedling production of Pak Choy. Therefore, further study needs to be done to identify the hydroponic solution without inorganic nutrients towards the organic vegetable production.
Potensi Sumberdaya Alam Hayati Kawasan Mangrove Pasar Banggi Kabupaten Rembang Sebagai Objek Ekowisata Kusaeri -; Sapto P. Putro; Jafron Wasiq
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3955

Abstract

Kawasan Mangrove Pasar Banggi merupakan salah satu tegakan mangrove yang masih baik dan sudah direboisasi sejak tahun 1960-an. Kawasan tersebut memiliki sentra persemaian mangrove yang cukup dikenal oleh masyarakat luas di Pulau Jawa. Penelitian ini bertujuan untuk mengetahui kondisi vegetasi dan kualitas lingkungan mangrove serta menyusun rekomendasi strategi pengembangan ekowisata mangrove di kawasan tersebut. Metode penelitian menggunakan deskriptif analitik melalui pendekatan kuantitatif. Hasil penelitian menunjukkan bahwa hutan mangrove Pasar Banggi seluas 14,88 ha dalam kondisi cukup baik dengan identifikasi jenis mangrove yang ditemukan Avicennia marina, Rhizophora apiculata, Rhizophora mucranata, Rhizophora stylosa, Sonneratia alba, dan Sonneratia caseolaris. Indeks Nilai Penting (INP) untuk vegetasi mangrove tertinggi terdapat pada jenis Rhizophora mucronata sebesar 281,82%, dan Indeks Kesesuaian  Wisata Mangrove pada 9 lokasi (plot) diperoleh data 61,54% yang masuk kedalam kategori “sesuai bersyarat”. Strategi pengembangan potensi mangrove berdasarkan Matriks Grand Strategy SWOT berada pada kuadran I yang berarti menggunakan “strategi agresif”. Dalam strategi ini yang diprioritaskan adalah penentuan master plan zonasi pemanfaatan secara terpadu, manajemen pengelolaan objek ekowisata yang profesional serta membangun komitmen semua pihak dalam menjaga kelestarian lingkungan.Mangrove areas of Pasar Banggi is one of the mangrove stands has been reforested since the 1960s. The region has a mangrove nursery centers well known by the public on Java Island. This study aimed to determine the condition of mangrove vegetation and environmental quality. It also aimed to make a recommendation for mangrove eco-tourism development strategy. The research method used was descriptive qualitative analytical approach. The results showed that mangrove forests of Pasar Banggi was 14.88 ha in good condition with the identification of mangrove species found were Avicennia marina, Rhizophora apiculata, Rhizophora mucranata, Rhizophora stylosa, Sonneratia alba, and Sonneratia caseolaris. The importance value index for the highest mangrove vegetation found in Rhizophora mucronata by 281.82%, and travel suitability index of mangrove at 9 locations (plots) acquired 61.54% of data that goes into the category of “conditionally appropriate”. Mangrove potency development strategy was based on SWOT Matrix Grand Strategy was in quadrant I, which means using “aggressive strategy”. The strategy priority was the determination of the zoning master plan for integrated data collection, the professional management of ecotourism object and to raise the commitment of all parties to keeping environment sustainability.
Effects of BAP and Lighting Duration on Banana (Musa paradisiaca cv. Raja Bulu) Micropropagation Benyamin Yosafat Manurung; Prita Sari Dewi; Murni Dwiati
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 3 (2021): December 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i3.25173

Abstract

This study aims to determine the best concentration of 6-Benzilaminopurine (BAP) and lighting duration for Raja Bulu banana cultivar micropropagation. The explant was taken from Pagubugan Village, Cilacap Regency, Central Java. The experiment was arranged as a Randomized Completely Block Design (RCBD) with two factors. The first factor was BAP, with concentration used were 0; 2.5; 5; 7.5 mg/L and the addition of Indole-3-acetic acid (IAA) 0.5 mg/L for all treatment. The second factor was lighting treatment. All explants were put in dark incubation for two weeks followed by light duration treatment: 12 hours of light/day and 16 hours of light/day for the next two weeks. The addition of BAP increased the number of shoots, but did not affect time for shoot initiation, shoot height, number of leaves and number of roots. The best BAP concentration was 2.5 mg/L with an average number of 3.2 shoots/explant. Scalps were formed in the treatment of BAP 5 mg/L and 7 mg/L after four weeks of incubation and has the potential to become new shoots. Nevertheless, the lighting duration did not affect all observed variables.
Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria Muhammad Evy Prastiyanto; Fandhi Adi Wardoyo; Wildiani Wilson; Sri Darmawati
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23600

Abstract

The multi-drug resistance (MDR) bacteria is a global health problem that causes high mortality every year. Therefore, novel antibacterial agents are needed from natural biological sources. This research aimed to investigate the antibacterial activities of various crude extracts of Averrhoa bilimbi against MDR bacteria. The antibacterial activity was calculated based on the use agar well diffusion assay and the minimum bactericidal concentration (MBC) using Mueller–Hinton broth in a microdilution method. Bacteria from wells were subcultured using inoculating loop onto a 5% sheep BAP. The best antibacterial activity, calculated as the most widely inhibitory zone and the smallest MBC values. The ethanolic extract showed antibacterial activity against the all MDR bacterial test in the agar well diffusion assay (10-14.5 mm inhibition diameter). The MBC of water extract against ESβL + CR Pseudomonas aeruginosa showed the best antibacterial activity (12.5 mg/mL). The fruit of bilimbi was shown to be potentially developed as antibacterial agents, especially for MDR strains. Further in vivo research and discovery of action mode are needed to shed light on their antibacterial effects. This study can provide new information about the benefits of bilimbi as a source of natural antibacterial againts MDR-bacteria
Enhancing Students’ Logical-Thinking Ability in Natural Science Learning with Generative Learning Model Henni Riyanti; Suciati Suciati; Puguh Karyanto
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.16612

Abstract

The logical-thinking ability is one of the important competencies of 21st century that should be empowered. It is one of the aspects in a cognitive science domain that has not been implemented effectively in teaching and learning in the class. Then, this study aimed to analyzed the influence of generative learning model to enhance students' logical-thinking ability. This study was also conducted by using a quasi-experimental study with pretest-posttest non-equivalent control group design. The sample was chosen by using simple random sampling technique. This study involved two classes which had 67 participants consisting of 34 students in Class VIII.9 as an experimental group and 33 students in Class VIII.7 as a control group. The researcher used the instrument of logical-thinking ability formulated in the form of multiple choice tests with five alternative answers that had been tested for its validity and reliability. The analysis of data employed t-test using SPSS 21. The result indicated that the t-count t-table (2.44 1.99) and p-value = 0.017 (p 0.05). It could be concluded that the application of generative learning model resulted in a significant influence on students' logical-thinking ability. The result of the analysis could be used by the schools for evaluating the quality of natural science learning in order to encourage students’ logical-thinking ability.
Description of Skin Anatomical Structures of Wistar Rats Exposed to X-Rays Radiation Lisdiana -; Agus Ernawati
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3536

Abstract

The research was aimed to find out a profile of an anatomical structure of the Rattus norvegicus skin after exposed to X-ray radiation. Research was performed by treating the 20 Rattus norvegicus at the age of 1.5 months. The weight rats were weighed approximately 100 ± 13 g grouped into four treatments with different dose of X-ray radiation. The four treatments were 0 mgy (control), 50 mgy, 100 mgy, and 150 mgy X-ray radiation. The variable in this research was a dose of X-ray radiation and the anatomical structure of the rattus norvegicus skin. The data obtained were analyzed with qualitative description. The research results after exposure of X-ray radiation for 5-days showed that there was no damage on the skin macroanatomy. Whereas, the observation in the skin microanatomy showed that there was a damage, e.g. thinning of the epidermis, cell picnosys, cell injury, and hemoragic. The result indicated that the different dose of X-ray radiation affected the skin anatomy structure. The X-ray radiation exposure at 100 mGy on skin microanatomy were caused a thinning of the epidermis in stratum corneum layer, picnosys on the nucleus, cell injury and hemoragic.Penelitian ini bertujuan untuk mengetahui gambaran struktur anatomi kulit tikus (Rattus norvegicu) strain Wistar setelah terpapar radiasi sinar-X. Sebanyak 20 ekor tikus umur 1,5 bulan dengan berat badan sekitar 100 ± 13 gram dikelompokkan ke dalam 4 perlakuan yaitu perlakuan dosis radiasi sinar-X sebesar 50 mGy, 100 mGy dan 150 mGy serta 1 kelompok kontrol. Paparan radiasi dilakukan selama 5 hari. Variabel penelitian ini adalah dosis paparan radiasi sinar-X dan struktur anatomi kulit. Data yang diperoleh dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan bahwa secara makroanatomi kulit tikus tidak terlihat kerusakan, tetapi secara mikroanatomi terdapat kerusakan berupa penipisan epidermis, piknosis sel, jejas sel, dan hemoragik. Hal tersebut dikarenakan besarnya dosis radiasi mempengaruhi terhadap perubahan struktur anatomi kulit. Paparan radiasi sinar-X dosis 100 mGy, menimbulkan kerusakan kulit tikus secara mikroanatomi berupa penipisan epidermis dilapisan stratum korneum, piknosis inti, jejas sel dan hemoragik.
Sea Urchin (Echinoidea) Distribution and Abundance in the Intertidal Zone of Bengkayang Regency Andi Ristanto; Ari Hepi Yanti; Tri Rima Setyawati
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.9763

Abstract

Sea urchins is the member of Echinoidea. This species can be found in tropical marine water to the poles. The study aimed to know the types, the abundance and spread of sea urchins in Bengkayang Regency. The research, used the transect method. Each station consisted of 3 transects with a length of 50 meters from the shore into the sea with the distance of 10 meters among the transects. This research obtained five species consisting of three species of the Diadema (genus) and two species of the Echinotrix (genus) i.e. Diadema antillarium, D. savignyi, D. setosum, Echinotrix calamaris and E. deadema. The density of sea urchins in Lemukutan Island ranges from 273 – 453 ind/Ha. The Density of sea urchins on Penata Kecil island ranges from 167 - 347 ind / Ha and on Penata Besar Island has a range from 307 - 387 ind/Ha. The highest diversity of sea urchins was found in Penata Kecil Island of (1.2355). The distribution of sea urchins in Lemukutan and Penata Besar Island was categorized into clumped and evenly distributed, while the distribution of sea urchin on Penata Kecil Island is included in the clustered and random categories. Distribution of sea urchins on the island is influenced by several factors such as sea water waves and food contained in the environment.
Antimalarial Herbal Plants in Kupang, Indonesia Ihwan Ihwan; Sari Hauliah Ahmad Koda
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.5811

Abstract

Malaria is an infectious disease caused by Plasmodium parasite transmitted through female Anopheles mosquito bite. This disease is harmful because Plasmodium is known to be resistant to antimalarial drugs. The emergence of malaria resistance has raised concerns among researchers to look for any alternative antimalarial drugs. There are some plants potentially used as antimalarial drugs that should bewell-documented through scientific research. This study aimed to get to know plant species that has antimalarial activity in Kupang region. Method: This research applied qualitative descriptive method while using exploration method specifically in the sampling process. Every sample is then taken for preservation as herbarium and identified based on its morphological features using determination key handbook. The result showed that there are 17 species of antimalarial plant in 10 observational sites. Those species of antimalarial plants i.e. Tithonia diversifolia, Momordica charantia, Cyperus rotundus, Strychnos lingustrina, Andrographis paniculata, Callicarpa longifolia, Tinospora crispa, Piper betle, Plectranthus scutellarioides, Alstonia scholaris, Carica papaya, Amaranthus spinosus, Artocarpus champeden, Cassia siamea, Azadirachta indica, Helianthus annuus and Blumea balsamifera. Therefore, the availability of the plant species with antimalarial activity in Kupang needs to be explored and developed as an alternative approach for the society in treating Malarial diseases.
Productivity of Arrowroots and Taro Grown Under Superior Teak Clones with Several Levels of Stand Density Daryono Prehaten; Suryo Hardiwinoto; Mohammad Na’iem; Haryono Supriyo; Widiyatno Widiyatno; Dian Rodiana
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.26428

Abstract

Perum Perhutani has an important role in providing food and wood for people. Diversity and diversification of food will reduce the need for one type of food, namely rice as a staple food. Some tuber and rhizome are source of alternative foodstuffs, such as taro and arrowroot. This study aimed to determine the suitability and productivity of arrowroot and taro planted under old superior teak clones with several levels of stand density. Arrowroot and taro were planted under 14-year-old teak stands with 4 levels of density. Both plants were planted in the form of an array, measuring of 3m x 15m, with a spacing of 75cm x 75cm between plants. They were arranged in Randomized Completely with Block Design (RCBD) placed in 4 blocks of observation as replications.  The results showed that under superior teak clone had the potential to be planted with arrowroot and taro. Teak stand density influenced significantly some characteristics of arrowroot (leaves number, leaves area, stem height, stem diameter, root length) and taro (stem diameter, tuber diameter). Arrowroot productivity per hectare increased with low density of teak stands, accounted for 55, 59, 80, and 88 kg respectively. Meanwhile, taro productivity from very high to low teak density were 365, 301, 523, and 426 kg/ha. The novelty of this study is that there is no record of intercropping studies on old superior teak clones, so this is among the first studies. The benefit of from this research result, it could be employed by Perhutani to support the Indonesian government in the national food security program
The Interaction between Marasmius pulcheripes J8 and Soil Fungi on Laccase Activity for POME Degradation Yohanes Bernard Subowo; Arwan Sugiharto
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20827

Abstract

A study on the effect of Marasmius  pulcheripes J8 and soil fungi interaction on the activity and ability of laccase to degrade palm oil mill effluent (POME) was previously conducted, and some Basidiomycetes fungi were identified capable. Therefore, the aim of this study was to determine the ability of Marasmius pulcheripes J8 to degrade POME in the presence of inducers, and interactions with soil fungi. Furthermore, 3 types of inducers were applied to elevate its laccase activity, which include CuSO4, sucrose and Ammonium tartrate. In addition, M. pulcheripes J8 was grown together with soil fungi, encompassing Aspergilus niger NK and Penicillium sp R 75, in order to boost the action. The results showed the highest laccase activity was in M. pulcheripes J8 pure culture on a PDB medium of 3566.04 U / mL.  Moreover, the POME decolorization was up to 74.25% after 20 days of incubation, and reduced COD level was 81%. Meanwhile, the addition of an inducer has never been performed before, and the outcome of this investigation showed the ability of M. pulcheripes J8 to degrade POME, and decrease environmental pollution. POME waste treatment using fungi is more affordable than other methods.

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