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Impact of Ethinyl Estradiol to Human Telomerase Reverse Transcriptase Activity on Complete Hydatidiform Mole Culture
Nurseta, Tatit;
Wijasa, Arsana;
Sitompul, Barlian
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology
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DOI: 10.32771/inajog.v4i2.82
Objective: To prove the effect of ethinyl estradiol as an activator on
human Telomerase Reverse Transcriptase (hTERT).
Method: The experimental study was conducted in vitro by using
culture of complete hydatidiform mole trophoblast cell. We exposed
the culture to ethinyl estradiol in varied doses and measured the
concentration of hTERT through RT-PCR quantitative. There were
40 specimens as control group and 20 specimens exposed to ethinyl
estradiol in different doses (10, 20, 40 and 80 mcg) as experimental
group. The activity of hTERT was measured by RT-PCR and the
concentration of it was assessed by ELISA. We analyzed the variables
using ANOVA, Turkey post hoc and Pearson correlation test.
Result: In control group, the concentration of hTERT was not detected.
Meanwhile, the concentration among different doses of
ethynil estradiol (10, 20, 40, 80 mcg) was 113,117.5; 114,507.6;
102,193.9; 127.546.1 amoles/ml, respectively. Among experimental
group, they were significantly different both using F test (ANOVA)
(p=0.001) and Turkey post hoc test (p=0.005). The correlation
among group was 0.84 which meant higher level of ethinyl estradiol
was correlated with higher activity of hTERT.
Conclusion: Ethinyl estradiol impacts to the increase of hTERT activity
on complete hydatidiform mole cell culture.
[Indones J Obstet Gynecol 2016; 4-2: 93-96]
Keywords: complete hydatidiform mole, ethinyl estradiol, human
Telomerase Reverse Transcriptase (hTERT)
Female Sexual Function after Vaginal Delivery with Episiotomy and Cesarean Section
Jembawan, I Made W
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology
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DOI: 10.32771/inajog.v4i2.407
Objective: To determine the difference of sexual function after vaginal
delivery with episiotomy and cesarean section in Sanglah Hospital,
Denpasar.
Method: This research was conducted using cross sectional method.
Sample was collected using consecutive sampling, starting from October 2011-September 2012. Our sample consists of 86 women, 43 post-episiotomy and 43 post-cesarean section. Sexual function was assessed using FSFI (Female Sexual Function Index). Total score was analyzed using independent t-test and difference of sexual function was tested using Chi-square, with significance level p0.05). There was no significant difference between the two groups in term of sexual arousal and lubrication, with p-value 0.160 and 0.67, respectively. However, we found significant difference in other domains, namely desire (p=0.014), orgasm (p=0.045), satisfaction (p=0.018), pain (p=0.02), and total FSFI score (p=0.006). Sexual dysfunction was found in 18.60% of the episiotomy group and 2.33% of the cesarean section group, with p=0.030.
Conclusion: Female sexual dysfunction was found to be significantly
different between women post vaginal delivery with episiotomy and
women who had cesarean section.
[Indones J Obstet Gynecol 2014; 4: 199-203]
Keywords: cesarean section, episiotomy, female sexual function
A1298C Polymorphism of Fetal Methylenetetrahydrofolate Reductase (MTHFR) Gene as a Risk Factor for Spontaneous Abortion
Sulfiana, Isah;
T Chalid, St Maisuri;
Farid, Retno B;
Rauf, Syahrul;
Hartono, Eddy
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology
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DOI: 10.32771/inajog.v4i2.77
Objective: To investigate the role of A1298C polymorphism of fetal
methylenetetrahydrofolate reductase (MTHFR) gene in spontaneous
abortion.
Method: The case control study design recruited 96 subjects in Siti
Fatimah and Pertiwi mother and child hospital, Dr. Wahidin Sudirohusodo,
Pelamonia, Bhayangkara, Syekh Yusuf, Haji and Labuang
Baji hospital from March to September 2014. All subjects fulfilling
the inclusion criteria were taken tissue samples from mothers experiencing
spontaneous abortion and blood samples from normally
born baby. The data were analyzed using Pearson chi-square with
significant rate of 5% (p
Transforming Growth Factor β1 and Tropoelastin Expression in Uterine Prolapse
Alvilusia, Alvilusia;
Fauzi, Amir;
Azhari, Azhari;
Wresnindyatsih, Wresnindyatsih;
Saleh, Irsan
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology
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DOI: 10.32771/inajog.v4i2.78
Objective: To know the correlation of the expression of transforming
growth factor beta (TGF-β1) and tropoelastin in uterine
prolapse.
Method: A cross-sectional study of 30 subjects suffered from uterine
prolapse in the Department of Obstetrics and Gynecology Dr. Mohammad
Hoesin hospital Palembang. The study was conducted
since December 1st, 2014 until July 31st, 2015. The sample was from
the sacrouterine ligament and immunohistochemical examination
was conducted to see the expression of TGF-β1 and tropoelastin.
Result: Of the 30 subjects obtained, the expression of TGF-β1 was on
30 subjects consisting of 18 (60%) for weak expression and 12
(40%) for strong expression. Meanwhile, the strong tropoelastin expression
was on 18 subjects (60%) and weak tropoelastin expression
on 12 subjects (40%). There was a positive correlation between
TGF-β1 and tropoelastin expression with moderate correlation
(p=0.014; r=0.44).
Conclusion: There is a positive correlation between the TGF-β1 and
tropoelastin expression of sacrouterine ligament in uterine prolapse
with moderate correlation.
[Indones J Obstet Gynecol 2016; 4-2: 70-74]
Keywords: transforming Growth Factor Beta 1, tropoelastin, uterine
prolapse
Prevalence of Postpartum Anxiety and Depression after Intrapartum Oxytocin
Valentine, Grace;
Kayika, I Putu G
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology
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DOI: 10.32771/inajog.v4i2.75
Objective: To know the prevalence of postpartum anxiety and depression
in patients who received intrapartum oxytocin.
Method: Across-sectional observational study was conducted in Dr.
Cipto Mangunkusumo hospital. Observation used Edinburgh Postnatal
Depression scale and Beck Anxiety Inventory before delivery
day, on the first and fourteenth day of postpartum.
Result: Of 112 patients, we found the prevalence of mild and moderate
anxiety were 94.6% and 5.4%. There were no anxiety women
before delivery and on the first day of postpartum. The prevalence
of mild and moderate anxiety on the fourteenth day of postpartum
were 83% and 16.9%. There was no severe anxiety found at those
time. On the other hand, the prevalence of postpartum depression
on the first and fourteenth day of postpartum were 31.3% and
32.1%. There was no association between exogenous oxytocin and
postpartum anxiety also depression. In multivariate analysis, we
found that women with low self image were more prone to postpartum
anxiety (OR 0.16, 95% CI 0.06-0.46). Meanwhile, postpartum
depression was associated significantly to self image (OR 0.17, 95%
CI 0.07-0.83), low income (OR 10.35, 95% CI 1.72-62.45) and pregnancy
plan (OR 0.17, 95% CI 0.06-0.53)
Conclusion: The patients who received intrapartum oxytocin are
more prevalent to have mild anxiety. The prevalence of depression
before delivery day, on the first and fourteenth day of postpartum
are similar. In statistic, there is no relationship between intrapartum
oxytocin administration and postpartum anxiety or depression.
[Indones J Obstet Gynecol 2016; 4-2: 59-63]
Keywords: anxiety, depression, oxytocin intrapartum, postpartum
Profile of Maternal Referral Cases
Indarti, Junita;
Ocviyanti, Dwiyana;
Aditya, Reyhan
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology
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DOI: 10.32771/inajog.v4i2.76
Objective: To explore the demography of maternal referral cases in
Dr. Cipto Mangunkusumo Hospital (RSCM) along with the accuracy
of referral. We also aim to evaluate the types of referral, origin of referral,
referring healthcare facility and quality of referring healthcare
facility.
Method: The design of this study was a cross sectional design which
described the accuracy of obstetrics referred cases in Emergency
Unit Dr. Cipto Mangunkusumo Hospital from 2013 to 2014.
Result: The total referred obstetric cases in 2013 was 1,645 patients.
It was consisted of 1,307 appropriate (79.5%) and 338 inappropriate
(20.5%) referred cases. Primary healthcare and general
hospital were the most often referring cases to RSCM during two
consecutive years. The top three cases referred to RSCM in both
2013 and 2014 were preterm premature rupture of membrane
(PPROM), continued by severe preeclampsia and preterm labor.
Conclusion: The number of referral cases in Indonesia is considered
high, particularly in RSCM as the tertiary healthcare facility. There
are still a high number of inappropriate referrals originating from
primary healthcare facilities, pointing to the fact that the referral system
is not running according to design or plan. To improve the quality
of referral system, proper monitoring and evaluation of referral
should be performed by local health department.
[Indones J Obstet Gynecol 2016; 4-2: 64-66]
Keywords: maternal case, referral system
A1298C Polymorphism of Fetal Methylenetetrahydrofolate Reductase (MTHFR) Gene as a Risk Factor for Spontaneous Abortion
Sulfiana, Isah;
T Chalid, St Maisuri;
Farid, Retno B;
Rauf, Syahrul;
Hartono, Eddy
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
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Full PDF (86.87 KB)
|
DOI: 10.32771/inajog.v4i2.77
Objective: To investigate the role of A1298C polymorphism of fetal
methylenetetrahydrofolate reductase (MTHFR) gene in spontaneous
abortion.
Method: The case control study design recruited 96 subjects in Siti
Fatimah and Pertiwi mother and child hospital, Dr. Wahidin Sudirohusodo,
Pelamonia, Bhayangkara, Syekh Yusuf, Haji and Labuang
Baji hospital from March to September 2014. All subjects fulfilling
the inclusion criteria were taken tissue samples from mothers experiencing
spontaneous abortion and blood samples from normally
born baby. The data were analyzed using Pearson chi-square with
significant rate of 5% (p
Transforming Growth Factor β1 and Tropoelastin Expression in Uterine Prolapse
Alvilusia, Alvilusia;
Fauzi, Amir;
Azhari, Azhari;
Wresnindyatsih, Wresnindyatsih;
Saleh, Irsan
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology
Show Abstract
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Download Original
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Original Source
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Full PDF (577.898 KB)
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DOI: 10.32771/inajog.v4i2.78
Objective: To know the correlation of the expression of transforming
growth factor beta (TGF-β1) and tropoelastin in uterine
prolapse.
Method: A cross-sectional study of 30 subjects suffered from uterine
prolapse in the Department of Obstetrics and Gynecology Dr. Mohammad
Hoesin hospital Palembang. The study was conducted
since December 1st, 2014 until July 31st, 2015. The sample was from
the sacrouterine ligament and immunohistochemical examination
was conducted to see the expression of TGF-β1 and tropoelastin.
Result: Of the 30 subjects obtained, the expression of TGF-β1 was on
30 subjects consisting of 18 (60%) for weak expression and 12
(40%) for strong expression. Meanwhile, the strong tropoelastin expression
was on 18 subjects (60%) and weak tropoelastin expression
on 12 subjects (40%). There was a positive correlation between
TGF-β1 and tropoelastin expression with moderate correlation
(p=0.014; r=0.44).
Conclusion: There is a positive correlation between the TGF-β1 and
tropoelastin expression of sacrouterine ligament in uterine prolapse
with moderate correlation.
[Indones J Obstet Gynecol 2016; 4-2: 70-74]
Keywords: transforming Growth Factor Beta 1, tropoelastin, uterine
prolapse
Factors Related to the Number of Antral Follicles on InVitro Fertilization (IVF)
Raharja, Fransiskus C;
Suwiyoga, Ketut;
Wardhiana, IPG
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology
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DOI: 10.32771/inajog.v4i2.79
Objective: To determine factors which are related to the number of
antral follicles on infertile patients.
Method: This cross sectional study was conducted in In-Vitro Fertilization
(IVF) clinic of Graha Tunjung, Sanglah hospital, Bali. All fertile
patients following the IVF program were calculated the number
of antral follicles in both ovarian using transgene USG. This sample
was recruited by random sampling from April 1st, 2001 to April 30th,
2011. We analyzed the data using Chi square test through SPSS for
Windows 17.0 version.
Result: Of 102 samples, the mean of patients’ age was 32.9% (SD
4.6) years old. From 72 patients (70.6%) experienced above 3 years
of infertile period, the primary infertile was on 69 patients (67.7%).
There was a relationship between patients’ age and the number of
antral follicles significantly (prevalence ratio (PR) 1.41; 95% CI 1.11-
1.79). Meanwhile, the number of antral follicles and type of infertile
(PR 1.02; 95% CI 0.76-1.37) also infertile period (PR 0.95; 95% CI
0.72-1.27) were not associated significantly.
Conclusion: Patients’ age has an association with the number of antral
follicles on IVF.
[Indones J Obstet Gynecol 2016; 4-2: 75-77]
Keywords: age, infertile, infertile period, number of antral follicles
and type of infertile