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FaST : Jurnal Sains dan Teknologi
Published by Universitas Pelita Harapan
ISSN : -     EISSN : 25989596     DOI : -
Core Subject : Science, Social,
Computer Science & IT Environmental Science
Started in 2017, FaST publishes articles in the areas of science and technology, particularly in food technology, biology, electrical engineering, industrial engineering, mathematics and civil engineering. Articles are either reviews and/or research results.
Arjuna Subject : -
Articles 242 Documents
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DETEKSI AGLUTINASI SECARA OTOMATIS UNTUK UJI GOLONGAN DARAH TIPE ABO BERBASIS KERTAS Handono, Juli; Wijaya, Sastra Kusuma; Ibrahim, Anwar Soefi
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 1 No. 1 (2017): NOVEMBER
Publisher : Universitas Pelita Harapan

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ABSTRACT.  The research is the early study of development of automatic detection for  the paper-based agglutination devices as a determinant of ABO blood typing, namely the integration of the blood type test equipment based on paper chromatography and forward grouping method with agglutination detection through image processing. Paper test was made by Whatman No. 4 filter paper with three hydrophilic columns, the paper test pattern were printed by using laser printers and cutting process to make hydrophobic field, paper test printed were placed in waterproof cases that was printed with a 3D printer. Reagents anti-A, anti-B and Anti-D (rhesus) were immobilized on each hydrophilic columns. By measurement of optimization paper strips obtained the value of optimization volume of reagents immobilized and optimization of blood volume test, each 6 µl Anti-A, Anti-B and Anti-D were immobilized on hydrophilic columns and 40 µl amount of blood sample needed for testing. The preliminary study was produced image processing software for blood agglutination detection with Absolute Subtract Difference (ASD) method, it was designed to support single-board computer Raspberry Pi and mini camera for decision result of blood type on the display. The test results get a high accuracy by comparing the results with slide test method.
KARAKTERISASI NUGGET PINDANG IKAN-AMPAS TAHU YANG DITAMBAH TEPUNG TULANG IKAN SEBAGAI SUMBER KALSIUM Hardoko, Hardoko; Suprayitno, Eddy; Sulistiyati, Titik Dwi; Arifin, Alfin Adam
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 1 No. 1 (2017): NOVEMBER
Publisher : Universitas Pelita Harapan

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ABSTRACT.  The bones of the boiled fish contain high calcium that can be used for osteoporosis therapy, but less utilized in food products. This study was aimed to study the characteristics of boiled fish nugget mixed with okara as filler and added fish bone flour as a source of calcium. The method used was experimental method which was divided into 2 stages, namely the addition of okara 20, 30, 40% and the addition of fish  bone flour of 60, 80, 100 mesh with concentration of 10, 20 ,30%. The results showed that the addition of okara could increase the water content of the nuggets, but has not yet affected the texture of the boiled fish nugget. In general, boiled fish nugget which had the highest panelists’ acceptance was the one that was added with 30% okara. The addition of fish bone flour tends to decrease water content and increases the texture of hardness boiled fish nugget -okara. The addition of fish bone flour to boiled fish nugget that had the highest panelists’ acceptance was fish bone flour of 10% with size of 100 mesh. The boiled fish nugget -okara contains 5.8 mg/g of calcium and 5.11% dietary fiber.Keyword : boiled fish nuggets, fish bone flour, okara
Formulasi dan Uji Iritasi Gel Antibakteri Dari Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi Linn) Terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa [Formulation and Irritation Test of Antibacterial Gel from Star Furit Leaves (Averrhoa bilimbi Linn.) Ethanol Extract Againts Bacteria Staphylococcus aureus and Pseudomonas aeruginosa] Sutriningsih, Sutriningsih; Sagala, Zuraida; Marhamah, Marhamah
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 1 No. 2 (2018): MAY
Publisher : Universitas Pelita Harapan

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Starfruit (Averrhoa bilimbi Linn.) is containing several chemical contents including tannins, alkaloids, flavonoids, polyphenols and saponins, which can be efficacious as an antibacterial. The purpose of this study is determine stability of  gel and the effect of gel formulation ethanol extracted from leaves starfruit (Averrhoa bilimbi Linn.) against the antibacterial activity of Staphylococcus aureus and Pseudomonas aeruginosa. This is an experimental laboratory research where the formulation of ethanol extracted from the leaves of starfruit (Averrhoa bilimbi Linn.) which  obtained from the process of maceration with 96% of ethanol solvent formulated in a gel dosage form. Gel formulations of the ethanol extracted from leaves starfruit was made with various concentration of the extract including 35%, 50% and 65% with hidroksi propil metil cellulose ( HPMC) as the base. We used without-extract as the negative control and  0.1% of gentamicin ointment as the positive control. The result gel from antibacterial activity was then processed using the agar diffusion method .The use of a gel formulation  ethanol extracted from leaves starfruit affected the antibacterial activity of Staphylococcus aureus and Pseudomonas aeruginosa . The results showed that the ethanol gel extracted from leaves starfruit (Averrhoa bilimbi Linn.) had the best antibacterial activity, indicated by 65% gel concentration containing the ethanol extracted from leaves starfruit being able to inhibit the growth of Staphylococcus aureus that for 28.62 mm with greater barrier diameter and Pseudomonas aeruginosa for 19 mm. Irritation test to 20 female with age 20 - 30 years old man and woman with put it on the back of hands for 2 hours in 3 days and the result is no irritation indication happend . Gel stability was had test for 4 weeks and nothing change happend 
Kajian Prediksi 3-Dimensi Biomarker Kanker Payudara Dari Jalur Ekspresi LincRNA-ROR/MIR-145/ARF6 [3D Prediction of Breast Cancer Biomarker from The Expression Pathway of LincRNA-ROR/MIR-145/ARF6] Parikesit, Arli Aditya; Anurogo, Dito
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 1 No. 2 (2018): MAY
Publisher : Universitas Pelita Harapan

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According to WHO, breast cancer is one of the main causes of mortality in women. To overcome this malady, molecular biomedical research is carried out intensively. Anomalies in the lincRNA-RoR/miR-145/ARF6 expression pathway were found to play a very important role in breast cancer, especially in the type of Triple-Negative Breast Cancer (TNBC), which is the most dangerous variant of the deadly disease. Bioinformatics research has found the existence of non-coding RNA (ncRNA) in these expression pathways, whose interactions are worth studying with 3-dimensional prediction methods. The 3-D prediction method for biomolecules has been widely developed and has been successfully applied to DNA and protein. However, for the structure of RNA, it has just been developed, due to its low stability and very high dynamics on the biomolecule. Our aim is to apply the latest computational method for predicting the 3-dimensional structure of ncRNA, which can be applied as key information in biomedical application research. Extrapolation of kinetics and thermodynamic indicators of ncRNA ultimately yields the siRNA Linc-ROR 3-Dimensional structure and siRNA mRNA-ARF6, each having 13 and 8 hydrogen bonds. The existence of these hydrogen bonds is very important in maintaining the stability of the compounds and shows its efficacy as drug candidates. It is expected that preliminary information from the predicted 3-dimensional structure of ncRNA is useful for optimization of laboratory experiments in the field of crystallographic biomolecules.
Karakterisasi Protein Sorgum dan Upaya Peningkatan Jejaring Protein Sorgum Dengan Penambahan Glukosa-Oksidase [Characterization of Sorghum Protein and Improvement of Sorghum Protein Net-Working with Addition of Glucose-oxidase] Wulandari, Endah
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 1 No. 2 (2018): MAY
Publisher : Universitas Pelita Harapan

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Sorghum (Sorghum bicolor (L.) Moench) contains about 10% protein so it can act as a source of protein. Kafirin is a protein in sorghum distinguished on α, β, and γ-kafirin based on molecular weight differences, ease of extracting, and molecular structure. Characteristics of the sorghum protein and the ability of glucose-oxidase will form a protein network naturally between proteins or peptides resulting in polymerization. The objectives of the research were to fractionate protein of the flour of Zh-30 sorghum strain using five types of solvents. The protein fractions were analyzed for protein content and molecular weight by SDS-PAGE and evaluated for protein-network formation by glucose-oxidase, the result showed that flour protein of the sorghum Zh-30 strain consisted of five classes of proteins with a ratio of respectively albumin, globuline, kafirin 1, gluteline and kafirin 2 or cross-linked kafirin 11:11:42:7:29. Kafirin 1 content was 36.01%, kafirin 2 24.79%, gluteline 22.15%, globuline 12.81% and albumin 4.24% of total protein. Molecular weight of each protein fraction of the sorghum flour Zh-30 strain was respectively albumin 69.9 kDa, kafirin 1 which consisted α1-kafirin 22.4 kDa and α2-kafirin 23.5 kDa, globuline 121 kDa, gluteline 15.5 kDa and kafirin 2 which consisted of α1-kafirin 16.2 kDa, α2-kafirin 23.5 kDa, β-kafirin 24.6 kDa and γ-kafirin 53.6 kDa. Protein net-working was established when the glutelin fraction was treated with glucose-oxidase (MW 69 kDa from 15.5 kDa).
Kajian Pustaka : Pemanfaatan Mikroba Yang Berpotensi Sebagai Agen Bioremediasi Limbah Pewarna Tekstil [A Review : Utilization of Potential Microbes as Bioremediation Agent for Textile Dyes Waste water] Valerie, Valerie; Pinontoan, Reinhard
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 1 No. 2 (2018): MAY
Publisher : Universitas Pelita Harapan

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The rapid expansion of textile industry in Indonesia raises substantial concerns with regard to the disposal of excess dyes into the environment. The deleterious consequences of such issue can be alleviated through wastewater management by combining physical, chemical and biological approach. Biological treatment through bioremediation needs to be thoroughly considered and developed as it offers favorable attributes by being sustainable, inexpensive and environmentally friendly. In Indonesia, the employment of bioremediation technique, to treat textile dye effluent, has been extensively applied through the use of activated sludge. Apparently, studies describing the potential of various indigenous microorganisms to degrade textile dyes have continuously been reported. This paper proposed practical methods to isolate and select for potential indigenous microbes as well as their employment as bioremediation agent on dye contamination. Selections can be enhanced through the incorporation of bioinformatics analysis on the approach of microbiology. Appropriate systems are required to be carefully designed to optimized application on-site.
Analisis Resiko Gempa Bendungan Leuwikeris, Provinsi Jawa Barat [Seismic Hazard Analysis Leuwikeris Dam, West Java] Ariyandi, Fioliza; Harahap, Muhammad Riza
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 1 No. 2 (2018): MAY
Publisher : Universitas Pelita Harapan

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One of the dams in West Java is Leuwikeris Dam is being planned. It required an assessment in the form of seismic hazard analysis on Leuwikeris Dam. In this paper seismic hazard analysis was performed using probabilistic and deterministic methods. Where seismic events in the repeat periods of 145, 475 and 10000 years are considered in seismic hazard analysis by probabilistic methods. While the spectra target selection is based on CMS (Conditional Mean Spectrum). The results of this study show the design criteria for seismic risk Leuwikeris Dam on condition Operating Basis Earthquake (OBE) and Safety Evaluation Earthquake (SEE) in the form of acceleration time history. In short spectra t = 0.2 seconds value peak ground accelerations (PGA), respectively for 0,23g, 0,30g and 0,54g, while in the long spectra period t = 1.0 seconds value of peak ground accelerations (PGA) respectively of 0.16g, 0.19g and 0.26g, which will be used as input in conducting a dynamic analysis of Leuwikeris Dam.
Pemanfaatan Buah Leunca (Solanum nigrum L.) Dalam Pembuatan Minuman Sari Buah (Utilization of Black Nightshade (Solanum nigrum L.) in the Making of Fruit Juice) Halim, Yuniwaty; Effendi, Diana; Nurwitri, Caecilia C.
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 1 No. 2 (2018): MAY
Publisher : Universitas Pelita Harapan

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Black nightshade (Solanum nigrum L.) fruit contains many antioxidant compounds, however, utilization of black nightshade as food or beverage product has not been much studied. The research was aimed to determine sugar concentration and acid source used in black nightshade juice making to obtain a product that is acceptable for the panelists and to observe the black nightshade juice quality changes during storage. Sugar concentration used were 10%, 12.5%, and 15%. Acids used were from citric acid, belimbing wuluh (Averrhoa bilimbi) and lime (Citrus aurantifolia). It was found that the selected formulation was black nightshade juice added with 15% of sugar concentration and acid added from belimbing wuluh (Averrhoa bilimbi). This formula has acceptance value of 4.41± 1.357  and the IC50 value of 2118.0582 ± 96.3983 mg/L. Storage was done for 6 weeks in refrigerated temperature. During storage, quality of black nightshade juice decreased after 2 weeks, observed from decrease in its antioxidant activity, total phenolic and flavonoid compound.  
KARAKTERISASI FISIKO KIMIA DAN INHIBISI α-GLUKOSIDASE BERAS ANALOG DARI BUAH Rhizophora mucronata [CHARACTERIZATION OF PHYSICO-CHEMICAL AND α-GLUKOSIDASE INHIBITION OF ANALOG RICE FROM Rhizophora mucronata FRUIT] Hardoko, Hardoko; Alfiana, Devy; Puspitasari, Yunita Eka
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 2 No. 1 (2018): NOVEMBER
Publisher : Universitas Pelita Harapan

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ABSTRAKPenelitian ini untuk mengetahui karakteristik fisiko kimia dan inhibisi α-glukosidase  beras analog dari tepung buah R. mucronata, tepung singkong, dan tepung E. cottonii. Penelitian ini dilaksanakan pada bulan April - Oktober 2016. Metode eksperimen melalui perlakuan rasio tepung buah R. mucronata dengan Tepung singkong (60:40, 70:30, 80:20) dan penambahan tepung E. cottonii (0, 3, 5, 7%).  Pembuatan beras analog menggunakan extruder. Hasilnya menunjukkan bahwa perlakuan rasio tepung buah R. mucronata dengan tepung singkong dan penambahan tepung E. cottonii berpengaruh posistif nyata terhadap inhibisi Î±-glukosidase dan sifat fisiko kimia beras.  Semakin tinggi jumlah tepung  buah R. mucronata yang digunakan makin tinggi aktivitas inhibisi Î±-glukosidase dan menurunkan nilai IC50. Beras analog terbaik adalah yang terbuat dari tepung buah R. mucronata 60%, tepung singkong 40%, dan tepung rumput laut E. cottonii 5%. Beras ini mempunyai nilai IC50 33,42 ppm, kadar air 8,46%, kadar serat pangan 38,96%, berwarna merah (nilai oHue 44,86), cooking time 11,35 menit, volume pengembangan 135,09%, dan kadar pati 51,44%.
PEMBUATAN TELUR PINDANG DENGAN PENAMBAHAN DAUN JATI (Tectona grandis L. f.) DAN DAUN JAMBU BIJI (Psidium guajava L.) Handayani, Ratna
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 2 No. 1 (2018): NOVEMBER
Publisher : Universitas Pelita Harapan

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Pemindangan telur dapat menjadi upaya untuk meningkatkan daya simpan telur rebus dan keragaman makanan di Indonesia. Telur pindang merupakan produk pangan olahan tradisional  dengan kombinasi penggaraman dan perebusan dengan menggunakan bahan penyamakan protein. Telur pindang dibuat dengan lima rasio penambahan daun jati dan daun jambu biji (100: 0, 75:25, 50:50, 25:75, dan 0: 100). Sampel kemudian dianalisis fisikokimia yang meliputi kandungan tanin, warna, kadar protein, dan kadar lemak, jumlah lempeng total selama penyimpanan, dan uji organoleptik. Hasil penelitian menunjukkan bahwa perbedaan rasio daun jati dan daun jambu biji mempengaruhi total mikroorganisme dalam telur selama 24 jam penyimpanan, dan tingkat kecerahan telur pindang. Formulasi terbaik berdasarkan jumlah total lempeng dan uji organoleptik adalah telur pindang yang telah direbus dengan 2% daun yang terdiri dari 50: 50 daun jati: rasio daun jambu biji dan direndam pada air rebusan selama 12 jam. 

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