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Scientific Dental Journal
Published by Universitas Trisakti
ISSN : 25806548     EISSN : 2541321X     DOI : -
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology
SDJ has been published by Faculty of Dentistry, Trisakti University since September 2017. SDJ, a triennially published scientific journal, is an open access, peer-reviewed journal that supports all topics in Oral and Dental Sciences, including, but not limited to Oral Biology, Oral Pathology, Pharmacology, Biochemistry, Microbiology, Histology, Oral Anatomy, Dental Material, Conservative Dentistry/Endodontics, Orthodontics, Pedodontics, Prosthodontics, Periodontology, Oral Surgery, Oral Medicine, Dental Radiology and Public Health.
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Articles 25 Documents
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Microbiomics of Oral Biofilms: Driving The Future of Dental Research Chaminda Jayampath Seneviratne; Tanujaa Suriyanarayanan
Scientific Dental Journal Vol. 1 No. 1 (2017): September
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v1i1.2089

Abstract

Oral infectious diseases such as dental caries, periodontal disease, endodontic infections, oral candidiasis and peri-implantitis cause major health problems worldwide. All of these infectious diseases are associated with the biofilm growth mode of the oral pathogens. In the past, researchers often attempted to examine the association of single pathogens with particular dental diseases such as in the case of Streptococcus mutans acting as an aetiological agent for dental caries and the so-called “red-complex” bacteria for periodontal disease. However, with the recent advent of OMICS biology techniques such as genomics, transcriptomics, proteomics, it is possible to gain new insights into the host-microbial interaction, microbial community structure and composition in the oral cavity. The new studies on oral microbiomics can unravel the facets of the aetiopathology of oral diseases as never seen before. This mini-review will provide an history and overview of some of the existing DNA sequencing platforms employed to study the microbiomics of oral biofilms and the exciting future ahead for dental research.
The Addition of Pharmacological Treatment to Physiotherapy in Pain Reduction of TMD-myalgia Patients Carolina Marpaung; Hasti Parvaneh
Scientific Dental Journal Vol. 2 No. 1 (2018): January
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i1.2227

Abstract

Background: Myalgia is the most common cause of Temporomandibular Disorders (TMDs) pain, which has a dull and mild character. In some patients with high somatization, it can present itself as severe pain, which can complicate the treatment. Additional pharmacological treatment may thus be needed. Case Report: Two female patients came with similar symptoms but different pain intensities. The first patient had a high intensity of pain, which became severe during examination. The second patient had more complaints but showed typical symptoms of myalgia. Both received physiotherapy sessions, but for the first patient, we added analgesics to help the patient endure physiotherapy. Conclusion: Additional pharmacological treatments, especially analgesics, can be used in helping myalgia patients with high somatization to undergo physiotherapy.
Strawberry Extract’s Effects on Enterococcus faecalis and Porphyromonas gingivalis Biofilms in vitro Armelia Sari Widyarman; Stephanie Brigitta Widjaja; Erik Idrus
Scientific Dental Journal Vol. 1 No. 1 (2017): September
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v1i1.1911

Abstract

Background: Enterococcus faecalis (E. faecalis) and Porphyromonas gingivalis (P. gingivalis) are oral bacteria related to root canal infection and periodontal disease pathogenesis. Strawberries (Fragaria x ananassa) fruit are rich in vitamins and minerals, have antibacterial and antioxidant effects. Objective: This study investigated the inhibition effect of strawberry extract on monospecies and multispecies E. faecalis and P. gingivalis bacteria grown as biofilms in vitro. Methods: This study used E. faecalis ATCC 29212 and P. gingivalis ATCC 33277. It analyzed the effect of strawberry extract on bacteria biofilm formation using a biofilm assay on microplate wells. Five concentrations of strawberry extracts were used (100%, 50%, 25%, 12.5%, and 6.25%), and the inhibition effect was observed after a 1h, 3h, 6h, and 24h incubation period. Biofilms without the strawberry extract were used as the negative controls, and crystal violet and safranin (0.5%w/v) were used to count the biofilm mass. The biofilms grown on microplates were counted using an ELISA reader at 450 nm after 200 mL of 90% ethanol was added to attract the absorbed stain. The strawberry extract inhibition effectiveness on the biofilm formation of each bacterium tested was analyzed using one-way Anova, where p<0.05 was defined as a significant difference. Result: The strawberry extract inhibited the tested monospecies and multispecies bacteria biofilm formation. The optimal strawberry extract concentration for the inhibition of either monospecies biofilms was 100%. However, the optimal incubation time for the strawberry extract to inhibit the multispecies biofilm formation was 24h, which was the study’s biofilm maturity phase. Conclusions: The 100% strawberry extract concentration inhibited the formation of both the monospecies and multispecies E. faecalis and P. gingivalis biofilms. Future studies are needed to evaluate the potential of strawberry extract as an alternative dental therapy.
Isolation and Identification of Indonesian Lactobacillus reuteri strain from Saliva of Young Adults Armelia Sari Widyarman; Stella Pranoto; Citra Fragrantia Theodorea; Endang Winiati Bachtiar; Boy Muchlis Bachtiar
Scientific Dental Journal Vol. 2 No. 2 (2018): May
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i2.2840

Abstract

Background: Biofilms are involved in a wide variety of microbial infections, including dental caries and periodontitis. The use of probiotics has been a promising prevention and treatment modality with which to combat biofilm-related diseases in the oral cavity. The probiotic Lactobacillus reuteri has been proven to reduce gingivitis and plaque index inside the oral cavity. These bacteria can be found in the digestive system and also in the human oral cavity. Objective: The aim of this study was to identify L. reuteri in the saliva of Indonesian young adults. Methods: Forty saliva samples were collected from 18–24 year-old Indonesian subjects. DNA extraction was performed, and then, the identification of L. reuteri was accomplished using PCR. Six subjects showed positive results. The positive samples were cultured in Rogosa Agar for 24 hours at 37°C anaerobically. Several single colonies were further cultured separately in broth medium before DNA extraction and PCR identification were performed. The four thickest bands were selected for DNA sequencing. Results: An analysis performed using BLAST showed that two of the L. reuteri strains obtained from the Indonesian saliva isolates had 96% (isolate 3.11) and 95% (isolate 5.14) values. This confirmed the presence of new strains based on average nucleotide identity (ANI). The isolate strains of 3.11 and 5.14 have been registered at DDBJ/EMBL/GenBank under the accession number LC382415 and LC382416, respectively. Conclusion: L. reuteri novel strain can be isolated from the saliva of Indonesian young adults.  Further studies involving biochemical tests and phenotypic analysis are needed to better understand these new L. reuteri strains.
Consumption of Yogurt Containing Probiotic Bifidobacterium Lactis Reduces Streptococcus mutans in Orthodontic Patients Armelia Sari Widyarman; Shirley Trisna Yunita; Tjokro Prasetyadi
Scientific Dental Journal Vol. 2 No. 1 (2018): January
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i1.1913

Abstract

Background: Probiotic bacteria is commonly used as a food supplement intended to benefit the host by improving intestinal bacterial balance. Probiotics have also been investigated from the perspective of oral health. Objectives: The purpose of this study was to investigate the effect of daily intake of yogurt containing probiotic Bifidobacterium animalis subsp. lactis BB-12 (B. lactis) on salivary Streptococcus mutans (S. mutans) counts in patients undergoing fixed orthodontic treatment. Methods: Saliva samples were collected from each subject (n = 7; mean age, 21 years) using spitting method in centrifuge tubes at baseline and two weeks after daily probiotic yogurt consumption. B. lactis BB-12 and S. mutans ATCC 25175 were cultured in BHI-broth (37ºC, anaerobic conditions). After 48 hours incubation, the number of colonies on each dilution plate was used to extrapolate a standard curve. The total number of target DNA molecules were identified using Real-Time PCR followed by SYBR Green reagents and 16S rRNA gene specific primers S. mutans and B. lactis BB-12. Data were analyzed statistically using paired-sample t-tests. Results: Statistical evaluation indicated that there was a significant reduction in the presence of S. mutans before probiotic yogurt consumption, (4.73 ± 1.43) log10 CFU/mL and after two weeks of daily consumption of probiotic yogurt, (4.03 ± 0.77) log10 CFU/mL, p = 0.001. Moreover, no B. lactis was found in the saliva of any of the subjects before probiotic consumption, but after two weeks of consumption, B. lactis was found in the saliva of four subjects. Conclusions: Consuming probiotic yogurt containing B. lactis reduced the quantity of S. mutans in the saliva of subjects during fixed orthodontic treatment. Thus, the probiotic bacteria could be beneficial in improving oral health.
The Effect of Seal Bag Storage towards Dimensional Stability of Alginate Impression Material Octarina Octarina; Jesslyn Raharja
Scientific Dental Journal Vol. 2 No. 3 (2018): September
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i3.3097

Abstract

Background: The dimensional stability of alginate impression material is very important for obtaining an accurate cast. Seal bag storage is one way to maintain dimensional stability of the impression material. Objectives: This research aims to compare the dimensional stability of alginate impressions between imprESSIX Color Change (Alginate A), Algeniux Normal Set Major (Alginate B), and Aroma Fine Plus Normal Set (Alginate C). The alginate impressions were stored in seal bags for 0 min, 10 min, 3h, 24h, 72h, 120h, and 168h. Method: Sixty-three gypsum cast samples were obtained from a master die using 3 different alginate impression materials.  The alginate impressions were stored in separate seal bags for each of the seven different time intervals, then the samples were poured with gypsum type III.  The buccal cusp length from right first molar to left first molar was measured for each gypsum cast sample using calipers and compared with the master die. The data were analyzed with two-way ANOVA followed by post hoc LSD test. Results: The distance between the mesial buccal cusps of the right and left first molar was 52.280 mm for the master die.  Gypsum cast samples of alginate A and B showed shorter lengths compared to master die, but the difference was not significant (p>0.05). A significant difference in dimensional stability was observed for alginate C (p<0.05) after 120h (52.080 mm) and 168h (52.057 mm). Conclusion: Dimensional stability of alginate impression was influenced by alginate composition and seal bag storage time. Seal bag storage could hold the dimensional stability of alginate A and B up to 168h, but for alginate C, the stability only lasted for 72h. 
Pomegranate Juice Inhibits Periodontal Pathogens Biofilm in vitro Armelia Sari Widyarman; Olivia Puspita Suhalim; Dhara Nandary; Citra Fragrantia Theodorea
Scientific Dental Journal Vol. 2 No. 3 (2018): September
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i3.2572

Abstract

Background: Pomegranate (Punica granatum) fruits are commonly regarded as medicinal plant in Indonesia, and the polyphenols found in pomegranate juice (punicalagin and ellagic acid) have been shown to have antibacterial properties. Objectives: Using monospecies and multispecies biofilms, we sought to examine the effects of pomegranate juice on the viability of three periodontal pathogens: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Treponema denticola. Methods: Biofilm assays were performed using crystal violet. Pomegranate juice was obtained from pomegranates using a juicer, and the juice was then diluted into different concentrations with phosphate saline buffer. The three pathogens were cultured in both monospecies and multispecies plates. Pomegranate juice was then added to each biofilm well. These were then incubated for 1h, 6h, or 24h, after which the optical density (OD) of the biofilm mass was measured using a microplate-reader (490 nm). Biofilm without treatment was used as a negative control and 0.2% chlorhexidine gluconate as a positive control. Data were analyzed with a one-way ANOVA; the level of significance was set at p<0.05. Results: Compared to the negative control, biofilm mass was significantly decreased after treatment with pomegranate juice across all concentrations and incubation times, for both monospecies and multispecies biofilm (p<0.05). The best results were achieved with P. gingivalis biofilm, with 100% concentration (OD 0.34 ± 0.03); A. actinomycetemcomitans, 50% concentration (OD 0.22 ± 0.01); and T. denticola, with 25% concentration (OD 0.87 ± 0.08), as well as with a multispecies biofilm with a 50% concentration in 1h incubation time (OD 0.09 ± 0.02). Conclusion: Pomegranate juice effectively inhibited the biofilm formation of P. gingivalis, A. actinomycetemcomitans, and T. denticola. Pomegranate juice may therefore be used as an alternative therapy in preventing periodontal disease. Additional research should explore this effect in an environment that mimics oral cavities.
International Prosthodontics: Two Decades of Discussion James Dudley
Scientific Dental Journal Vol. 2 No. 3 (2018): September
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i3.2830

Abstract

Background: The perception that the specialty of prosthodontics has changed over time is subjective and largely based on personal belief, with little data to uphold this view in the literature. Objectives: The aim of this study was to compare the frequency of presentation categories at each International College of Prosthodontists Conference from 1999 to 2017 and identify trends. Methods: Available ICP Conference Programs were sourced from the ICP Administration and the titles of all presentations reviewed and catalogued into one of 13 presentation categories. The number of presentations in each category was expressed as a percentage of the total number of presentations for each conference and statistically analyzed for trends throughout the study period. Results: 69.61% of all presentations from all conferences were categorized as one of implant dentistry; materials, CAD-CAM and digital technologies; removable prosthodontics and edentulism; or fixed prosthodontics. 46.38% were categorized as implant dentistry or materials, CAD-CAM and digital technologies. The frequency of presentations of fixed prosthodontics significantly decreased while the frequency of implant dentistry; maxillo-facial prosthodontics; geriatric dentistry and medically compromised patients; and clinical case presentations and treatment planning significantly increased (p<0.05) over the study period. Conclusion: The ICP Conference presentation titles reviewed from 1999 to 2017 maintained a focus on implant dentistry and materials, CAD-CAM and digital technologies alongside a diverse range of other topics. Throughout the study period, the frequency of presentations of fixed prosthodontics significantly decreased while the frequency of presentation of implant dentistry; maxillo-facial prosthodontics; geriatric dentistry; and clinical case presentations and treatment planning significantly increased.
One Visit Relining Procedure in Patient with Loss of Vertical Dimension Niko Falatehan; Rebecca Gandhanya
Scientific Dental Journal Vol. 2 No. 3 (2018): September
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i3.2984

Abstract

Background: Dentists often encounter patients with worn dentures that still feel comfortable even though the patients have lost the supporting tissue heights due to physiological and pathological resorption. Loss of denture retention, stability, and occlusal vertical dimension are most often caused by alveolar bone resorption. Dentures that are loose because of poor adaption to the supporting tissues should be relined. Chair-side procedures provide immediate resolution, avoiding the edentulous period of time accompanying laboratory relines. Case Report: A 70-year-old male edentulous patient came to the Prosthodontic Clinic, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia. The patient had been wearing the upper and lower dentures for approximately eight years. The patient had alveolar ridge resorption, which causes loss of retention, stability and vertical dimension. After the problems were thoroughly diagnosed and corrected, a chair-side denture relining with an open-mouth technique was used. Conclusion: Direct relining in a single visit helped the complete denture to regain its retentiveness, allowing for prolonged the use of the worn denture. It also adjusted occlusal vertical dimension to improve the occlusal support and esthetics.
The Knowledge Level of Temporomandibular Disorders Pathophysiology by Dental Practitioners in Jakarta, Indonesia Christopher Ryan Aliwarga; Carolina Marpaung
Scientific Dental Journal Vol. 2 No. 3 (2018): September
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i3.3205

Abstract

Background: Temporomandibular disorder (TMD) is a common term used to describe the problems related to masticatory muscles, joints, and supporting tissues. TMD commonly causes orofacial pain and has been the subject of considerable research in the field of dentistry. The treatment chosen for patients who have TMD is largely determined by the knowledge of general dental practitioners. Objectives: The purpose of this research was to assess the understanding of TMD pathophysiology by such practitioners. Methods: Questionnaires were distributed randomly to general dental practitioners in private offices and hospitals in the area of Jakarta, Indonesia. The answers on the returned questionnaires were compared to those of TMD experts. Results: Of the 117 questionnaires distributed to general dentists, 105 were returned (89.7% response rate). The results showed an overall level of knowledge of TMD pathophysiology of 25%. Conclusion: The majority of general dental practitioners in Jakarta lack knowledge of TMD pathophysiology

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