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INDONESIA
Indonesian Aquaculture Journal
ISSN : 02150883     EISSN : 25026577     DOI : -
Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.
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Articles 334 Documents
STUDIES ON Aeromonas hydrophila BACTERIA DISEASES IN WILD AND CULTURED ELVER EEL (Anguilla bicolor) Noviana Dewi; Isti Koesharyani
Indonesian Aquaculture Journal Vol 12, No 2 (2017): (December, 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.11 KB) | DOI: 10.15578/iaj.12.2.2017.77-82

Abstract

This study was performed to detect Aeromonas hydrophila infection in wild and cultured elver eel (Anguilla bicolor). In total, 20 live elvers (10 wild and 10 cultured) were collected and subjected to Aeromonas hydrophila tests. Polymerase chain reaction (PCR) was done for an accurate identification of Aeromonas hydrophila using a universal primer and detection of the aerolysin gene in A. hydrophila using a specific primer. The virulence of A. hydrophila was determined using a pathogenicity test injection. The results showed that A. hydrophila could be detected using PCR and amplification of 685bp DNA. A. hydrophila could also be confirmed to contain the aerolysin gene of 290 bp DNA which could be a virulence indicator. Pathogenicity test revealed that LC50 was estimated to be 10.9 x 10 6.33. Histopathological changes were found in the abdominal and wounded muscles. Those changes were mainly in tissue ephitel cell hyperplasia. Based on the present study, A. hydrophila is a virulent bacteria in elver eel. A. hydrophila disease preventive measures need to be formulated. Elver eels should be tested for the A. hydrophila before restocking into farms.
MANIPULATION OF INCEPTISOLS POND BOTTOM SOIL THROUGH ADDITION OF ULTISOLS AND VERTISOLS FOR REARING OF RED TILAPIA (Oreochromis sp.) LARVAE Saberina Hasibuan; Bambang Djadmo Kertonegoro; Kamiso Handoyo Nitimulyo; Eko Hanudin
Indonesian Aquaculture Journal Vol 6, No 1 (2011): (June 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.508 KB) | DOI: 10.15578/iaj.6.1.2011.59-70

Abstract

The pond bottom soil of Inceptisols mixed with Ultisols (I-U) and Vertisols (I-G) with the proportion of (70:30); (50:50); (30:70) showed the improvement of clay fractions >30% compared with the control (100% Inceptisols). These mixtures were given basal fertilizer (quail droppings manure with dosage of 2 tons/ha/month), Urea, and SP36. Cultivation system was divided into two stocked: fish ponds with 50 fish/m2 and ponds without fish. The best mix with the basal fertilizer was further treated with fertilization treatment (1 ton/ha/month of quail droppings manure) 3 times within 42 days of larval rearing. Physical and chemical soil analyses showed an increase of total soil porosity (> 50%), decrease of SG, BD, and permeability (medium), pH > 5, the CEC > 20 cmol (+) kg-1, and the C/N ratio < 6.5. The proportion of the best mix of 30% I : 70% U and 50% I : 50% G with 3 times of the fertilization frequency gave significant improvement of water quality, chlorophyll-a, diversity of plankton, benthic algae, fish growth, and total biomass. The presence of mineral montmorillonite on a mixture of 50% I : 50% G was a very good sign of the bottom soil fertility.
STUDY PROPAGATION OF GSDIV (GROUPER SLEEPY DISEASE IRIDOVIRUS) IN CULTURED GF (GRUNT FIN) CELL Ketut Mahardika; Indah Mastuti
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (861.531 KB) | DOI: 10.15578/iaj.8.2.2013.121-126

Abstract

Grouper Sleepy Disease Iridovirus (GSDIV, genus Megalocityvirus) remain unsolved in Indonesian aquaculture. A GSDIV propagating study had been conducted to elaborate the capability of GSDIV replication in GF cell line. GSDIV isolate derived from spleen of diseased grouper fish were infected to GF cells line. GSDIV-infected GF cells were characterized by enlargement cells as CPE (cytophatic effect) which floated in cultured-medium. CPE-GSDIV was positively confirmed by PCR test. GSDIV-viral concentrations were ranging between 102.80-105.80 TCID50/mL. The newly passage GF cells were more sensitive to GSDIV which was shown by the abundance of CPE. GSDIV derived from GF cells were caused 10%-100% mortality of humpback grouper, Cromileptes altivelis.
‘YELLOW SYNDROME’ IN SCLERACTINIAN CORALS THROUGHOUT BINTAN DISTRICT, KEPULAUAN RIAU PROVINCE, INDONESIA Ofri Johan; Agus Budianto
Indonesian Aquaculture Journal Vol 12, No 1 (2017): (June 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.156 KB) | DOI: 10.15578/iaj.12.1.2017.37-42

Abstract

Coral disease surveys were conducted in Bintan, Kepulauan Riau Province. The purpose was to identify the abundance of corals showing signs of Yellow Syndrome (YS) disease and to describe similar pathological signs to that of AYBD throughout Bintan District. Three belt transects (2 m x 50 m in size) were set up to determine the abundance of coral reef attacked by YS disease. Line intercept transects were used to determine the percentage of live corals in the surveyed areas. The survey showed that the YS disease syndrome attacked 8 different genera i.e. Acropora, Montipora, Porites, Pavona, Turbinaria, Favia, Platygyra, and Favites. The highest attack happened at Mapur Island (0.06 kol/m2) on Porites lutea, Turbinaria peltata, T. mesenterina, Acropora bruggemanni, and Pavona frondifera. The survey also indicated that there may have been at least two types of YS i.e. the first type caused by a boring and/or over-growing sponge species and the second type caused by a kind of pathogenic microbe. Regardless the causal agent of YS, the severity of YS attack on coral urged immediate action to be undertaken and should include initial microscopic and histology examinations. Based on this initial microscopic and histology examinations it was found out that YS bears a close resemblance to the Arabian Yellow Band Disease. This study, however, argued that the word “disease” may have been incorrectly used without identifying a specific causal agent.
THE IMPACTS OF FASTING PERIODS ON FOOD INTAKE, GROWTH RATE, COMPENSATORY GROWTH, AND EFFICIENCY OF FEED UTILIZATION IN BLUE TILAPIA (Oreochromis aureus) REARED IN BRACKISH WATER PONDS Priadi Setyawan; Adam Robisalmi; Nunuk Listiyowati; Raden Roro Sri Pudji Sinarni Dewi; Imron Imron
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1364.039 KB) | DOI: 10.15578/iaj.7.2.2012.149-156

Abstract

Blue tilapia (Oreochromis aureus) has known as euryhaline species refers to wide range of salinity tolerance. The pure population or hybrid with other strain of tilapia can be a good candidate in marginal coastal land use. Tilapia is the most important aquaculture species in Indonesia. Refers to FAO 2010, Indonesia is the third biggest of tilapia production after China and Egypt. One of the main problems in aquaculture is their feed as the major cost in fish farming. Various techniques had carried out to reduce of feed cost such as improving fish quality, sex reversal and feed management. This research aimed to determine of compensatory growth in tilapia as one technique in feed management. Fry obtained from natural spawning in freshwater pond. Acclimatization in 20 ppt made in aquarium for 4 days after one month reared in hapas. Fish reared in 2 m x 1 m hapas with five treatments and three replications. This research had conducted at brackish water pond in Yogyakarta. The treatments is A: one day fasting and six days feeding (1/6), B: 2/5, C: 3/4, D: 4/3 and E is control. Results showed that the biggest of average weight gain is treatment of E (68.36 g) followed by A (66.38 g), B (62.44 g), C (43.56 g), and D (27.30 g) respectively. One-way ANOVAs analysis with 95% of interval confidences continued with Tukey’s Pairwaise comparison showed that nothing significant different between E, A, and B. The biggest of daily growth rate and specific growth rate is E (0.75 g/day and 4.68 %bw/day) followed by A (0.73 g/day and 4.65 %bw/day); B (0.69 g/day and 4.58 %bw/day); C (0.48 g/day and 4.18% bw/day) and D (0.29 g/day and 3.65% bw/day) respectively. Survival rate of A is 73.00% followed by E. 72.00%, C. 71.00%, D. 69.69%, and B. 67.00% respectively. Feed conversion ratio of D is 0.83 followed by C (0.87), B (0.98), A (1.16), and E (1.41). Food efficiency ratio of D is 127.06% followed by C (118.75%), B (106.09%), A (88.87%), and E (73.38%). These results indicate that fasting of one day and two days has no significant effect on fish growth. Treatment A and B is the better treatment for fish culture refers to the better value of FCR, FER, and total weight gain.
THE EFFECT OF PROBIOTIC ON IMMUNITY IMPROVEMENT IN THE FRY AND SPAWNER PRODUCTION OF PACIFIC WHITE SHRIMP Litopenaeus vannamei Haryanti Haryanti; Ahmad Muzaki; Sari Budi Moria Sembiring; Fahrudin Fahrudin; I Gusti Ngurah Permana; Ida Komang Wardana
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.536 KB) | DOI: 10.15578/iaj.9.2.2014.133-146

Abstract

In order to improve immune system of Pacific white shrimp L. vannamei from any kinds of diseases, various probiotic agents were tested such as Bacillus sp. BC, Alteromonas sp. BY-9 and mixed of both. The probiotics were cultured and added directly to the larval rearing tank (106 cfu/mL equal to 0.5 liter/m3) up to PL-12. In cultured shrimp spawners, probiotic was mixed with feed coated with chitosan, and without adding probiotic as control. In the present experiment, shrimp fry and spawners were reared in tank capacity of 5 m3 and 8 m3. Immunity response was analyzed by quantitative value of the immunity related gene expression with RTqPCR. The results showed that survival rate of shrimp fry cultured with mixed probiotic was 63.16% (PL-8) and 53.46% (PL-12), while control 13.35% (PL-8) and 12.48% (PL-12) respectively. The number of shrimp hemocytes starting from 420 to 530.5 x 104 cells/mL and after challenged with WSSV (for 72 hrs exposure) between two treatments of Alteromonas sp. BY-9 and Bacillus cereus BC was not significantly different P>0.05 (402.5 and 432.5 x 104 cells/mL), while in mixed probiotic and control treatments were 391.75 and 229.0 x 104 cells/mL, respectively. The analysis of immunity gene expression revealed that the use of probiotic from larval stages up to spawner has been proved in increasing immunity responses quantified from ProPO activating system (prophenoloxidase/proPO) as much as 1.6-2.0 fold, clotting system (transglutaminase, clotting protein) of 1.5-2 fold, Antimicrobial Peptide System (anti-LPS factor, crustin, lysozyme, penaiedin2) of 1.5-2.8 fold, and Antioxidant defense mechanism (superoxidase dismutase/SOD, glutathione peroxidase/GPx) of 1.8-2.0 fold compared to control.
OBSERVATION ON SKELETAL DEFORMITY IN HATCHERY-REARED RED SPOTTED GROUPER, Epinephelus akaara (Temmick et Schlegel) FROM LARVAL TO JUVENILE STAGE Eri Setiadi; Seiichi Tsumura
Indonesian Aquaculture Journal Vol 2, No 1 (2007): (June 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.89 KB) | DOI: 10.15578/iaj.2.1.2007.35-45

Abstract

Skeletal deformity is a significant problem in fish culture. The skeletal deformities in red spotted grouper from yolk-sac to juvenile stages were examined through clearing and staining of the cartilage and bone using Alcian Blue and Alizarin Red S. The overall results showed that the pattern of incidence of deformities showed an increase from preflexion to juvenile stages. The rate of deformities based on ten elements of bone from preflexion to juvenile stages were as follows: vertebral (42.6%—9.0%), dorsal proximal radials (4.8%—25.2%), neural spine (0%—8.4%), haemal spine (0%—6.8%), hypural (1.3%—5.4%), anal proximal radials (0%—5.4%), epural (1.3%—4.9%), arypural (2.0%—4.5%), lower jaw (1.3%—2.5%), and upper jaw (0%). Vertebral and dorsal proximal radials were recognized as the most susceptible parts to deformation. The main types of bone deformity were lordosis, scoliosis, fusion, shortening, branching, supernumerary elements, and saddleback syndrome. Development of saddleback syndrome was detected initially in preflexion stage, which was accompanied by deformity of the neural spines, dorsal proximal radials, and disposition of the distal radials and dorsal spines in later life stages. The skeletal deformity encountered during the larval rearing period could be caused by water surface tension.
CLONING OF ProAV PROMOTER ISOLATED FROM TIGER PRAWN Penaeus monodon Andi Parenrengi; Alimuddin Alimuddin; Sukenda Sukenda; Komar Sumantadinata; Muhammad Yamin; Andi Tenriulo
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.086 KB) | DOI: 10.15578/iaj.4.1.2009.1-7

Abstract

Promoter is a specific DNA sequence involved in the transcription of a particular gene. It is usually located in the upstream of the gene they regulate. Isolation and characterization of promoter is essentially needed in order to establish the sequence analysis and transcription factor that are used in the regulation of gene expression. The research was conducted to analyze the characteristics of Penaeus monodon anti viral gene promoter (ProAV) towards generation of auto-transgenic tiger prawn, P. monodon. ProAV promoter was isolated by PCR (Polymerase Chain Reaction) method and the purified DNA fragment was cloned into pGEM-T Easy cloning vector. The promoter sequence was characterized by using BLAST-N and Genetyx version 7 softwares. The results showed the success in isolating a promoter from tiger prawn of 368 bp in length. BLAST-N analysis showed that the sequence of isolated promoter has high similarity (95%-98%) compared to the other promoters in the GeneBank. The study revealed the existence of important transcription factors (TATA box, MRE, TCF-1, and other potential regulatory elements) are identified in the promoter sequence.
COUNTERGRADIENT VARIATION IN GROWTH OF BARB (Barbonymus balleroides Val. 1842) DOMESTICATED AT DIFFERENT ALTITUDES Jojo Subagja; Vitas Atmadi Prakoso; Otong Zenal Arifin; Endang Haris Suhud
Indonesian Aquaculture Journal Vol 13, No 1 (2018): (June, 2018)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.102 KB) | DOI: 10.15578/iaj.13.1.2018.7-12

Abstract

Barb (Barbonymus balleroides Val. 1842) is one of the native species found in many rivers of Asian countries, including Indonesia. This species had higher commercial value compared with other fish species popular among consumers. In terms of supporting its domestication, information regarding the optimal aquaculture system of the fish is needed, one of which is its rearing location. Currently, there is limited information on rearing the fish at different locations with different altitude. This research aimed to obtain the growth of barb fingerlings reared in three locations with different altitudes. The study was conducted in the ponds located at Cijengkol area (ASL<200 m), Maleber (200 m<ASL<400 m), and Ciherang (ASL>400 m), West Java. Fingerlings of domesticated barb (total length 4.48 ± 0.10 cm; weight 0.95 ± 0.06 g) were stocked in three fixed net cages (size 2 m × 2 m × 1 m) in a pond (40 m × 20 m) at each location. Fish were fed with commercial feed (30% protein) of 5% biomass three times per day during 120 days of rearing. Water quality parameters observed were temperature, dissolved oxygen, and pH. Measured parameters were length gain, weight gain, specific growth rate, average daily growth, biomass gain, feed conversion ratio, and survival rate. The results showed that the fish reared in Maleber showed the best growth and feed conversion ratio compared to other locations (P<0.05). Meanwhile, no significant differences were found on the survival rate within all treatments. The growth of barb fingerlings is more optimal if reared in midland areas which have suitable temperature ranges for their growth.
THE DYNAMIC OF LANDSCAPE CHANGE AT COAST AREA, IN LABAKKANG SUBDISTRICT PANGKEP REGENCY, SOUTH SULAWESI Andi Gusti Tantu; Soemarno Soemarno; Nuddin Harahab; Akhmad Mustafa
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.294 KB) | DOI: 10.15578/iaj.7.1.2012.69-77

Abstract

Development is a changing process to improve human standard of life that strongly related to the activities of natural resource utilization. It is frequently found that these activities change the ecosystems and their resources. Eventually, these changes wil l give ser ious impacts to the envi ronments. The most distinct environmental problems are caused by resident migration to the coastal areas, coastal developments, and land limitation. Coastal reclamation is an example of human effort to respond to the land limitation in the coastal areas as observed in coastal areas of Labakkang Subdistrict. Reclamation activity is doing along the coast of Pundata Baji Village, whereas, in other villages, cutting off of mangrove areas as an alternative land expansion is increasingly practiced by the local communities. This Research was aimed to observe the landscape change from 1980 to 2010 at coast area in Labakkang Subdistrict. Each review considers Landsat Image Map (acquired in 1980); Landsat Image Map (acquired in 1990); Landsat Image Map-7 Enhanced Thematic Mapper Plus (ETM+) acquired in 2000); Spot Image 4 (acquired in 2005); and Spot 4 LAPAN (acquired in 2010). Result of research indicates that at coast area of Labakkang Subdistrict in 1980, there are 248.3 ha of mangrove vegetation, 2,756.63 ha inundation, and 4,157.0 ha open land. In 1990, there are 234.2 ha mangrove vegetation, 2,251.63 ha embankment, 933.2 ha rice field, and 582.0 ha open land. In 2000, there are 218.3 ha mangrove vegetation, 2,848.1 ha embankment, and 3,579.2 ha rice field. In 2005, it has 121.4 ha mangrove vegetation, 3,762.6 ha embankment, and 2,306.2 ha rice field. In 2010, it is found 48.9 ha mangrove vegetation, 5,029.35 ha embankment, and 749.98 ha rice field.