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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 344 Documents

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RAPD FINGERPRINTING OF THREE SPECIES OF GROUPER (Epinephelus spp.) FROM MAKASSAR STRAIT, SOUTH SULAWESI, INDONESIA Andi Parenrengi
Indonesian Aquaculture Journal Vol 1, No 2 (2006): (December 2006)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Grouper belonging to the epinepheline serranid family has promising aquaculture potential for food and ornamental fish production. Random amplified polymorphic DNA (RAPD) technique was employed in this study to determine the genetic variability and species differentiation of groupers from genus Epinephelus (E. areolatus, E. merra, and E. suillus). Groupers (N=52) were collected from Makassar Strait, South Sulawesi, Indonesia. Of 34-screened RAPD primers, ten primers (OPA-02, OPA-06, OPA-08, OPA-10, OPA-15, OPA-16, OPA-17, OPA-18, OPA-19, and CA-05) were selected to generate the RAPD fingerprinting of groupers. Results indicated that the ten primers revealed different RAPD profile among three species. Similarity index obtained by pairwise comparison was 0.62 ± 0.07 for E. areolatus, 0.58 ± 0.11 for E. merra and 0.80 ± 0.11 for E. suillus. Polymorphism and genotype number detected were 51.3% ± 8.7% and 3.8 ± 0.6 in E. areolatus; 56.8% ± 14.6% and 4.1 ± 1.1 in E. merra; and 60.2% ± 11.8% and 3.8 ± 0.9 in E. suillus, respectively. Genetic distance level of individuals ranges from 0.19—0.42, 0.14—0.50, and 0.02—0.30 for E. areolatus, E. merra, and E. suillus, respectively. The genetic distance between E. merra and E. areolatus (D=0.52) was closer compared with E. merra and E. suillus (D=0.67). A total of 33 RAPD markers differentiating the three species of groupers were detected. The study presents the RAPD technique as the useful genetic marker for assessment of genetic diversity and species differentiation in groupers.

IMPROVEMENT OF NON SPECIFIC IMMUNE SYSTEM ON COMMON CARP (Cyprinus carpio) AGAINST KOI HERPESVIRUS DISEASE (KHVD) BY ADDITION OF ASCORBIC ACID ON FISH DIET: A FIELD SCALE STUDY Taukhid Taukhid; Desy Sugiani; Angela Mariana Lusiastuti
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The research with the aim to know the optimal feeding frequency of supplemented ascorbic acid (microencapsulated vitamin C CFC-90) on the dose of 750 mg/kg feed to control Koi Herpesvirus (KHV) disease infecting common carp has been done in field condition. Fish were reared in floating cages with the size of 3.5 m x 3.5 m x 2.0 m and stocking density of 1,250 fish/cage with the size range of ± 10 g/fish. The treatments applied in the research were: (A) daily application, (B) every 3 days application, and (C) without vitamin C addition as the control. Fish test were challenged to KHV infection on the mid cultivation by cohabitation method in the laboratory scale for 2 weeks. Examination on behavior, clinical sign, and mortality of fish test conducted daily. The results showed that the highest survival rate was found on the application of vitamin C every 3 days (60.16%); and followed by every day (52.00%), and the lowest was found on the control group (47.36%).

COMPENSATORY GROWTH OF Oreochromis niloticus SELECTED STRAIN FROM BOGOR, WEST JAVA Vitas Atmadi Prakoso; Kurniawan Kurniawan
Indonesian Aquaculture Journal Vol 12, No 2 (2017): (December, 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Inefficient feed management strategy in aquaculture will increase the fish production cost. One of the most effective strategies to solve this problem is through a better understanding of the compensatory growth of cultured fish. O. niloticus BEST tilapia strain (total length: 7.23 ± 0.11 cm mean ± SD; Body weight: 7.04 ± 0.08 g mean ± SD) were reared in aquariums at 26.3 ± 1.4oC for 10 weeks. During the experiment, the control group was fed twice a day. The other two groups were deprived of food for one and two weeks and then fed twice a day during refeeding period. At the end of the experiment, the fish deprived for one week had a body weight, biomass and specific growth rate that were not significantly different from the control group. The body weight, biomass and specific growth rate of fish deprived for two weeks were significantly lower than the other groups. This study revealed that concentrations of ash and lower concentrations of protein and lipid on the deprived groups were higher compared to those without feed deprivation. Mortality of fish was lower than 9% and not significantly different among the treatments. Fish aggressive behavior was the main reason for injuries and death. Given the results, BEST tilapia strain was only able to reach complete growth compensation not longer than one week deprivation period. The results of the present study could be applied as basic information for further research on feeding management of BEST tilapia strain.

THE UTILIZATION OF THE KINDS OF LIVE FOOD ON CLOWN LOACH FISH JUVENILES (Chromobotia macracanthus Bleeker) Nina Meilisza; Rina Hirnawati; Sulasy Rohmy; Agus Priyadi; Jacques Slembrouck
Indonesian Aquaculture Journal Vol 6, No 1 (2011): (June 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Domestication and mass production technology of clown loaches fish have been developed by the Research Institute for Ornamental Fish Culture, Depok and the Institute de Recherche pour le Developpement (IRD). In the process, some constraints has been found and one of them was the cost of production and sustainable supply of Artemia as the only one of live food which used for clown loaches post-larvae until 2-3 months old juvenile. To solve this constraints, the use of other live feed as a substitute or replacement of Artemia should be applied, caused by it research of the utilization of the kinds of live food on clown loach juvenile need to be done. This research aims to determine the kinds of live food which be recommended for the substitution of Artemia on clown loaches fish juvenile (Chromobotia macracanthus Bleeker). Research carried out using 1 month old clown loach juveniles designed into the Complete Randomized Design in two factors namely are class of fish size S (35-50 mg), M (55-70 mg), L (75-90 mg) and the kinds of live food in the form of Artemia as a control, Moina, mini maggot, blood worms (Chironomus), and silk worms (Tubifex). Three levels treatment by class of fish size factor and five levels treatment by the kinds of live food factor produce 15 kinds of interactions with duplications. The research was conducted at recirculation system for 8 weeks to 3 months old juveniles. Observations were carried and measuring the growth parameters including weight, total length of fish, as well as survival rate parameters based on the number of fish that live at the end of the research. The support is done by measuring the water quality of pH, DO, temperature, ammonia, conductivity, and nitrites, proximate analysis of feed also performed. The two of research step analyzed by ANOVA (Analysis of Variance) and continued by Tukey test.

EXPRESSION OF GROWTH HORMONE (PhGH) GENE AND ANALYSIS OF INSULINE-LIKE GROWTH FACTOR I (IGF-I) PRODUCTION IN AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC F-1 Huria Marnis; Bambang Iswanto; Rommy Suprapto; Imron Imron
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

We have previously produced F-1 transgenic of African catfish from crosses between founder transgenic female and non transgenic male. The aim of this study was to evaluate distribution and expression PhGH growth hormone gene transgenic African catfish organs and to measure the concentration of IGF-I in plasma. Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, gill, and eye. Transgene expression levels were analyzed using the method of quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR). Plasma samples were analyzed for Insuline-like Growth Factor (IGF-I) using Enzyme Linked Immunosorbent Assay (ELISA) method. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish (F-1). Liver exhibited the highest level of PhGH mRNA (23 x 106 copies). The plasma IGF-I levels in transgenic individuals were not significant than non transgenic. The higher level of exogenous PhGH gene expression may not represent the production of IGF-1.

ARE COPEPODS VIABLE OPTIONS AS LIVE FOOD IN AQUACULTURE HATCHERIES? Wa Iba; Hatim Albasri
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

This present paper overviews the use of copepods in aquaculture. Some culture methods and nutritional values also described to decide whether copepods are viable and reliable to be used as live food in aquaculture hatcheries. Copepods have been known to have higher nutritional value than Artemia and rotifers. In aquaculture, they have been used to fed various species of marine finfish with better results in terms of growth, larval survival and pigmentation compared to some fish larvae fed on other live feeds. However, culturing copepods in intensive systems to harvest high number of copepods is not well established yet due to lack of funding and knowledge. Meanwhile extensive and semi intensive systems are possible to transfer parasites and diseases from wild environment. Furthermore, nutritional value can not be controlled in such systems.

DISTRIBUTION OF TROPICAL EEL GENUS Anguilla IN INDONESIA WATER BASED ON SEMI-MULTIPLEX PCR Melta Rini Fahmi; Laurent Pouyaud; Patrick Berrebi
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Tropical eels living in Indonesian waters are known to be composed of several species, but their real listing together with their distribution ranges need to be established. The main difficulties are the very high number of islands with perennial rivers where these species are living during the growth phase of their life cycle. It is difficult, sometimes impossible, to determine the species using morphological characters, moreover on glass eels. In order to establish the geographic distribution of tropical eels of the genus Anguilla in Indonesian waters, a total 1,115 specimens were collected between 2008 and 2012. Sample collection was done in the growth habitats that are rivers and estuaries by commercial nets of different categories according to the fish size. All samples were identified genetically using the recently developed semimultiplex PCR method. We recognized four species and subspecies with wide distribution: Anguilla bicolor bicolor, Anguilla bicolor pacifica, Anguilla marmorata, and Anguilla interioris; two species with limited distribution, close to endemism: Anguilla celebesensis and Anguilla borneensis and one subspecies Anguilla nebulosa nebulosa that is only spread in river flowing into Indian Ocean.

THE USE OF SEAWORM MEAL IN MATURATION DIET AS PARTIAL SUBSTITUTION OF FRESH DIET FOR POND REARED TIGER SHRIMP BROODSTOCK Asda Laining; Usman Usman; Rachman Syah
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The purpose of this experiment was to evaluate the effects of using seaworm meal in artificial diet as partial substitution of freshfeed for maturation of tiger shrimp. This experiment started by growing-out tiger shrimp with initial weight around 60 g for four months until reaching maturation phase where shrimp weight were over 90 g for female. Tiger shrimp was selected and stocked into 10 ton concrete tank with stocking density of 50 shrimps with ratio of female : male of 1:1. Dietary treatments were different levels of seaworm meal at 0% (SW0), 10% (SW10) and 20% (SW20). SW0 was positive control without seaworm meal but breeder was fed with frozen seaworm. Test diets were fed as a combination of 60% test pellet and 40% fresh feed. Artificial insemination was carried out for all females before ablation to obtain fertile eggs. Results showed that after ablation, number of female matured was highest in group fed SW10 (13 breeders) and the lowest in female fed control group (7 breeders). Number of female spawned was also highest in female fed SW10 and the lowest was in positive control. Fecundity was very low in all treatments ranged from 12,000-79,700 eggs/spawn. Even though female bearing spermatophore through insemination, number of spawning hatched was very low, only three spawned in each of SW0 and SW10 and two spawned in SW20. Based on number of breeders matured and spawning rate, breeder fed with SW10 gave better performance than other two diets. Technique of artificial insemination needs to be improved to increase the number of fertile eggs.

THE USE OF REMOTE SENSING, REGRESSION QUANTILES, AND GIS APPROACHES FOR MODELING OF SCALLOP LARVAE: A Case Study in Funka Bay, Hokkaido, Japan I Nyoman Radiarta
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

In the development of scallop cultivation in Japan, larvae collection and propagation become an important factor. Although the monitoring program has been conducted, modeling of species distribution is becoming an important tool for understanding the effects of environmental changes and resources management. This study was conducted to construct a model for providing estimation of the scallop larvae distribution in Funka Bay, Hokkaido, Japan using the integration of remote sensing, Regression Quantile (RQ) and Geographic Information System (GIS)-based model. Data on scallop larvae were collected during one year spawning season from April to July 2003. Environmental parameters were extracted from multi sensor remotely sensed data (chlorophyll-a and sea surface temperature) and a hydrographic chart (water depth). These parameters together with larvae data were then analyzed using RQ. Finally, spatial models were constructed within a GIS by combining the RQ models with digital map of environmental parameters. The results show that the model was best explained by using only sea surface temperature. The highest larvae densities were predicted in a relatively broad distribution along with the shallow water regions (Toyoura and Sawara to Yakumo) and the deeper water areas (center of the bay). The spatial model built from the RQ provided robust estimation of the scallop larvae distributions in the study area, as confirmed by model validation using independent data. These findings could contribute on the monitoring program in this region in order to distinguish the potential areas for an effective spat collection.

SUSCEPTIBILITY OF DIFFERENT CELLS TO RED SEA BREAM IRIDOVIRUS (RSIV) Ketut Mahardika; Indah Mastuti
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

RSIV is an isolate virus in the genus Megalocytivirus (family Iridoviridae) that has been reported to be pathogen in more than 31 marine fish species in East Asia. The aim of study was to know the susceptibility of several cultured cells to RSIV. RSIV inoculum was inoculated onto cultured cells and then incubated in 25oC. Routine observation of cytopatic effect (CPE) was carried out for 7 days and harvested cells were prepared for virus titration and electron microscopy (EM). The result showed that RSIV grew and propagated in GF (grunt fin), KF-1 (koi fin) and BF-2 (barfin flounder) which caused cytophatic effect as cel ls enlargement. However, RSIV did not propagated on EPC (epithelioma papulosum cyprini), FHM (feathed minnow) and EK-1 (eel kidney) cells. The virus titer were 105.3 TCID50/mL in GF cells, 103.8 and 4.3 TCID50/mL in KF-1, 103.6 and 3.8 TCID50/mL in BF-2, and 7 102.1 TCID50/mL in EPC, FHM and EK-1. The EM observation revealed formation of enlarged cells containing hexagonal virus particles with 140-160 nm in diameter. These results indicated that GF was cultured cell to be optimal for replication of isolate RSIV derived from Ise bay, Mie, Japan.

Page 9 of 35 | Total Record : 344

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Contact Name
Ofan Bosman

Contact Email
Ofan Bosman

Phone
-

Journal Mail Official
iaj.puslitbangkan@gmail.com

Editorial Address
-

Location
Kota adm. jakarta pusat,

Dki jakarta

INDONESIA