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Bioscientia Medicina : Journal of Biomedicine and Translational Research

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Published by Universitas Sriwijaya

ISSN : - EISSN : 25980580 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

BioScientia Medicina is an open access international scholarly journal in the field of biomedicine and translational research aimed to publish a high-quality scientific paper including original research papers, reviews, short communication, and technical notes. This journal welcomes the submission of articles that offering a sensible transfer of basic research to applied clinical medicine. BioScientia Medicina covers the latest developments in various fields of biomedicine with special attention to medical sciences, Traditional Herb, genetics, immunology, environmental health, toxicology, bioinformatics and biotechnology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health.

Arjuna Subject : Kedokteran - Anatomi

Articles 1,209 Documents

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Methods of Selection and Maintenance of Experimental Animals for Biomedical Research Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 1 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i2.227

A B S T R A C TResearch is an activity carried out based on scientific principles and methodssystematically to obtain information, data, and information from relatedsubjects, with understanding the theory and proving assumptions and / orhypotheses. The results obtained are conclusions that can be applied orbecome additional knowledge for the progress of science. However, researchactivities must still respect the rights and dignity of research subjects.Health research includes biomedical, epidemiological, social, and behavioralresearch. Some health research can be done in vitro, using mathematicalmodels, or computer simulations. If the research results are to be used forhumans, further research is needed using living materials (in vivo) such ascell lines and tissue cultures. However, to observe, study, and conclude alloccurrences in living things as a whole, experimental animals are neededbecause experimental animals have a value for each part of the body andthere are interactions between these body parts.

Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 5 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i5.228

ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.

Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 4 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v5i4.229

Western blotting is an important technique used in cell and molecular biology. Using the western blot, researchers can identify specific proteins from the complex mixture of proteins extracted from cells. This technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target proteins using appropriate primary and secondary antibodies to visualize. This paper will attempt to explain the techniques and theory behind western blot, and offer several ways to solve the problem

The Association between Aromatase Gene Polymorphism Cyp19 Val 80 and Endometriosis Risk Syifa Alkaf; Ziske Maritska; Venny Patricia
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 5 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i5.230

Background: Endometriosis implant has been known to express aromatase enzyme, Cytochrome p450 that catalyzes androgen into estrogen. It causes local estrogen production, leading to increased estrogen level, and subsequently triggers endometriosis lesion. CYP19 gene resided at chromosome 15q21.1 is the biggest component of aromatase coding cytochrome p450 enzyme. Objective: To identify relationship between aromatase gene polymorphism CYP19 Val 80 and the risk of endometriosis. Methods: This is an observational case-control study using frozen DNA sample from women with endometriosis and/ or adenomyosis who had undergone laparotomy/ laparoscopy at Obstetrics and Gynecology Department Dr. Mohammad Hoesin General Hospital Palembang January-November 2013. Samples were amplified and cut by PCR-FRLP using Rsa1 restriction enzyme. Results were divided into A/A genotype (homozygote mutant), G/A (heterozygote mutant), and G/G (homozygote wild type). Data were analyzed by SPSS 21.0 version. Results: PCR-RFLP results for A/A genotype were 20 (21.3%) in endometriosis group and 8 (8.5%) in control group. G/A genotype were 18 (19.1%) in endometriosis group and 22 (23.4%) in control group. G/G genotype were 9 (9.6%) and 17 (18.1%) in endometriosis group and control group, respectively. There was significant increase risk of endometriosis in women carrying genotype A/A to those with genotype G/G with OR 4.722 (p<0.05). Conclusion: Polymorphism on aromatase gene CYP19 Val 80 A/A increases risk of endometriosis.

Klippel Trenaunay Syndrome: A Brief Overview Pande Ayu Naya Kasih Permatananda; I Gusti Agung Made Adnyana Putra
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 5 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i5.231

Klippel-Trenaunay syndrome is a rare congenital vascular disease. The pathogenesis of this syndrome is unclear, but it is thought that most cases are the result of somatic mutations that affect genes that play a role in vasculogenesis and angiogenesis. Some patients come with a triad of capillary malformation (hemangioma or port-wine stain), venous varicosities and bony or soft tissue hypertrophy. Clinical presentation of this syndrome can lead to significant morbidities and mortalities due to severe bleeding and emboli. Although the number of cases is low, a doctor must be able to distinguish Klippel-Trenaunay Syndrome from other rare vascular disorders. Parkes Weber syndrome is usually similar to Klippel-Trenaunay syndrome, except in the arterial malformations associated with capillary malformations and soft tissue to skeletal or bone hypertrophy. The diagnosis of Klippel-Trenaunay Syndrome is carried out clinically and is quite difficult to do even with experienced doctors because there is no precise pathognomonic test. There are several options in relation to the management of Klippel-Trenaunay Syndrome and non-invasive procedure is considered to be the most important of therapy modalities. Early diagnoses, progression monitoring, and proper intervention should be carried out for better prognosis and preventing complication.

Preclinical Test Methods with Animal Mouse and Rats Biomedical Research: Technical Guideline Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 2 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i2.232

A B S T R A C TTrials in pre-clinical trials are very complex multidisciplinary studies. Extrapolatingdata from animals to humans requires information from many fields of science. Frompre-clinical trials, more detailed information and data on efficacy and safety wereobtained, especially at doses equivalent to doses in humans, and the presence orabsence of cumulative effects and whether these effects could return to normal(reversible) after the administration of the test material was stopped. This test canbe used to predict the negative impact on humans if he is exposed to the materialfor a long time.

Anemia in Chronic Kidney Disease : Role of Hypoxia Inducible Factor Stabilizer Emilia; Zulkhair Ali
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 5 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i5.234

Anemia contributes to increased morbidity and mortality in chronic kidney disease patients. The pathogenesis of anemia in these patients is multifactorial, but the contribution of erythropoietin deficiency becomes greater as glomerular filtration rate declines which related to decreased nephron mass. The current standard of care includes supplemental iron, erythropoiesis-stimulating agents (ESA), and red blood cell transfusions, although each has drawbacks. Lately, concern has arisen following randomized clinical trials showing that higher hemoglobin targets and/or high ESA doses may cause significant harm including increasing cardiovascular and thrombotic events, and even death. Recent experimental and clinical studies show the promising efficacy of hypoxia inducible factor (HIF) stabilizer which stimulates endogenous erythropoietin production and enhance iron availability.

The Ethics of Gene Modification: Considerations of Gene Editing and Genetic Selection Murwani Emasrissa Latifah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 5 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i5.235

The field of hereditary qualities has offered ascend to innovation that will reform the natural sciences: the amazing quality altering device known as CRISPR-Cas9. Between clinical advancement, extreme new age solution for sicknesses and the danger of modifying human DNA in an irreversible manner, the lawful difficulties of applying CRISPR in the wellbeing setting, alongside the administrative and moral issues that may emerge should be drawn nearer with expanded duty. A primary objective or an assortment of purposes? Upgrade or quality altering to improve ordinary human characteristics, changes from altered genomes that may be acquired - repercussions of the new quality altering innovation could bring about adjusting human DNA. Current worldwide enactment and understanding, worldwide agreement worth seeking after regarding this matter and future guidelines required.

Recurrent Vulvovaginal Candidiasis Cyntya Sari Sovianti; Mutia Devi
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 5 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i5.280

Vulvovaginal candidiasis is a common fungal infection caused by Candida Sp, especially Candida albicans. Recurrent vulvovaginal candidiasis was defined as the occurrence of four or more episodes of vulvovaginal candidiasis in 12 months period. As many as 9% of women from various populations have recurrent vulvovaginal candidiasis. Vulvovaginal candidiasis affects the quality of life, mental health, and sexual activity. There are many predisposing factors that caused recurrent vulvovaginal candidiasis, such as genetics, host, habit, idiopathic and non-albican candida microbes. Management of recurrent vulvovaginal candidiasis includes elimination of predisposing factors; mycological culture diagnosis and identification of specific Candida species; followed by microbiological examination to confirm the sensitivity of the azole group to Candida sp. Further, oral, or topical therapy should be continued until the patient is asymptomatic and culture-negative. Patients should receive induction therapy followed by maintenance suppressive therapy for six months.

Epidemiology of Leprosy in Childhood: A Retrospective Study Rusmawardiana; Fifa Argentina; Indri Widya Sari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 5 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i5.309

Background: Children are the group most susceptible to Mycobacterium leprae infection. The proportion of new leprosy cases in children is indicator of successful leprosy elimination program from World Health Organization (WHO), so this study would like to know the epidemiology of pediatric leprosy at Dermatology and Venereology Polyclinic, Dr. Mohammad Hoesin Palembang. Methods: Retrospective study of all new cases of leprosy in children (0-17 years) who came to Dermatology and Venereology Polyclinic, Dr. Mohammad Hoesin Palembang from January 2017 to December 2020. Results: There were 10 pediatric patients (7.58%) from 132 new leprosy cases. Most cases (40%) were in the 15-17 age group. There are more male than female. Most types of leprosy are BL (60%) followed by TT (20%). Manifestations of hyperpigmented skin patches and nervous disorders, grade 1 disability, and type 2 leprosy reactions are mostly found in type BL leprosy. The duration of illness 6-12 months has a grade 1 disability (30%). The history of positive contact came from same household in 3 cases (30%) with 7 people (70%) domiciled in Palembang. All cases received Multidrug Therapy (MDT) treatment according to WHO guidelines. Conclusion: Found 10 new cases of leprosy in children in Dermatology and Venereology Polyclinic Dr. Moh. Hoesin Palembang for four years, especially in the 15-17 age group, with the most cases being type BL leprosy. Household contact still plays an important role in leprosy transmission.

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Contact Name Rachmat Hidayat

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Location Kab. ogan ilir, Sumatera selatan INDONESIA

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Contact Name
Rachmat Hidayat

Contact Email
dr.rachmat.hidayat@gmail.com

Phone
-

Journal Mail Official
dr.rachmat.hidayat@gmail.com

Editorial Address
-

Location
Kab. ogan ilir,

Sumatera selatan

INDONESIA