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INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Published by Universitas Nahdlatul Ulama Surabaya
ISSN : 26846748     EISSN : 26569825     DOI : https://doi.org/10.33086/ijmlst
Core Subject : Health, Social,
Health Professions Social Sciences
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 113 Documents
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The baseline levels of receptors and cytokines in phorbol 12-myristate 13-acetate-induced THP-1 monocyte to macrophage cells Wulandari, Sri; Agusti Sholikah, Tri; Jusup, Sinu Andhi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.7917

Abstract

Monocyte-derived macrophages regulate various aspects of inflammation. Phorbol 12-myristate 13-acetate (PMA)-induced THP-1 monocytes are widely used as monocyte-derived macrophage models suitable for studying the pathophysiology of inflammation and developing therapies. However, there is insufficient data concerning the baseline expression of pro-inflammatory receptors and the associated cytokine levels in THP-1-derived macrophage-like cells compared with their monocyte precursors. In this study, PMA-treated THP-1 cells were employed as models for monocyte-derived macrophage-like cells (M0). The mRNA levels of Toll-like receptors (TLR)4, receptor for advanced glycation end-product (RAGE), and TLR7 in THP-1 cells, both with and without PMA treatment, were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). ELISA was performed to quantify IL-6, TNF-α, and IL-18 levels in the THP-1 culture supernatant. The mRNA expression of TLR4, RAGE, and TLR7 was increased twofold in PMA-induced THP-1-derived macrophage-like cells compared to monocytes. IL-6 (p = 0.0277) and TNF-α (p = 0.0105) levels were significantly elevated in the culture supernatants of THP-1-derived macrophages. However, IL-18 levels did not show a significant increase. The upregulation of TLR4, RAGE, and TLR7 transcription was accompanied by elevated secretion of IL-6 and TNF-α during PMA-induced differentiation of THP-1-derived macrophage-like cells (M0), highlighting the polarization state and the specific role of macrophages in producing pro-inflammatory cytokines. This study provides baseline data on pro-inflammatory receptor expression and cytokine production in the THP-1-derived macrophage-like cells model for inflammatory research.
Role of chemerin and omentin in predicting ovulatory dysfunction in obese women with polycystic ovary syndrome (PCOS) Abdullah, Noor Najm; Alameri, Manar Abbas; Muhammed, Thikra Majid; Mohsein, Osama A.
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.7930

Abstract

Polycystic ovary syndrome (PCOS) is an endocrine condition that is characterized by ovulatory dysfunction, hyperandrogenism, and metabolic imbalances. The aim of the study was to assess the predictive ability of chemerin and omentin-1 in ovulatory dysfunction of obese PCOS women. The study is a case-control study conducted at Al-Nasiriyah General Hospital (Jan 2024 – Jun 2025) with a sample size of 150 obese and 70 normal weight women with PCOS and 50 healthy controls. The diagnosis of PCOS was made according to Rotterdam criteria (2003). They included participants aged 18 years to 35 years old, who were not on hormonal therapy in the recent past; pregnancy, endocrine and chronic diseases were excluded. Blood samples were fasted and serum was separated and stored in the refrigerator at 20°C. Hormonal (Follicle-Stimulating Hormone, Luteinizing Hormone, LH/FSH, testosterone, Sex hormone binding globulin), metabolic (glucose, insulin, HOMA-IR, triglycerides, HDL-C), and adipokine (chemerin, omentin-1) levels were measured using ELISA, spectrophotometry, and Cobas e411 kits (Roche, Germany).  There were no major differences in terms of age (p=0.63). BMI, waist circumference, menstrual irregularity and family history of PCOS were more prevalent among the obese PCOS women. There was an increase in the LH, LH/FSH ratio and the total testosterone and a decrease in SHBG. The most pronounced metabolic abnormalities were observed in PCOS obese, with lowest HDL-C, and progressively higher levels of metabolic abnormalities with increasing deficiency of ovulatory dysfunction and insulin resistance. There are significant differences in hormonal, metabolic, and ovarian changes in obese and non-obese PCOS women. High levels of chemerin and low levels of omentin-1 are closely related to insulin resistance and ovulatory dysfunction and suggest their use as predictive biomarkers and mediators of metabolic and reproductive pathophysiology in PCOS.
Study the association between myeloperoxidase gene single-nucleotide polymorphism G463A (rs2333227) and coronary artery disease in Iraqi patients Salem, Wisal Abdulrhman; Ali, Dawood Salman; Abbas, Hamid Jaddoa
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.8589

Abstract

Acute coronary syndrome (ACS) is a major cause of morbidity and death globally, which is caused by the multifaceted interaction between oxidative stress and inflammatory processes. The enzyme myeloperoxidase (MPO) is secreted by activated leukocytes and is a determinant of vascular dysfunction and unstable plaque. The genetic variation within the MPO gene, especially G463A (rs2333227), may increase enzyme expression and contribute to disease occurrence. The aim of the study was to examine the relationship between the MPO G463A polymorphism, circulating MPO levels, and the risk of ACS. The case-control study included 129 patients with ACS and 66 apparently healthy controls. Immunoassay techniques were used to measure serum MPO and high-sensitivity troponin I levels, and PCR-based methods were used to assess the MPO G463A polymorphism. The MPO level in serum was significantly higher in ACS patients than in controls (p < 0.001). The ROC analysis revealed an average diagnostic ability for MPO (AUC = 0.686, sensitivity for70.7%). There was a significant association between the MPO G463A polymorphism and ACS susceptibility (p > 0.05). Nonetheless, some genotypes were correlated with changes in MPO levels, and they may contribute to the severity of the diseases. An elevated serum MPO level is strongly correlated with ACS and can serve as a useful biomarker for its detection. Conversely, the MPO G463A polymorphism does not appear to have a significant impact on disease susceptibility but may affect individual differences in the inflammatory response.

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