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INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Published by Universitas Nahdlatul Ulama Surabaya
ISSN : 26846748     EISSN : 26569825     DOI : https://doi.org/10.33086/ijmlst
Core Subject : Health, Social,
Health Professions Social Sciences
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 105 Documents
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Potential of ethanolic extracts from seed kernel Indonesian cultivars mangoes as an anti-multidrug-resistant (MDR) Escherichia coli Ariyadi, Tulus; Qayimah, Sitti; Assa’diyah, Lulu’ Nur; Asyiah, Asyiah; Pratiwi, Hanum Adisty; Khairunnisa, Afifah; Hikmah, Annisa Nurul; Kusmita, Lia; Mutmainah, Mutmainah; Mukaromah, Ana Hidayati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.5837

Abstract

Escherichia coli is a type of bacteria that often found in wound infections. The use of antibiotics to treat these uncontrolled infections has resulted in the emergence of multi-drug resistant (MDR) strains of pathogenic bacteria.  This rapid increase in MDR E. coli strains has led to significant morbidity and mortality in human populations. Consequently, the exploration of natural antibacterial agents is urgently needed. One promising resource is mango extract, which is recognised for its antibacterial properties and is obtained through solvent extraction using 96% ethanol. This study aimed to determine the antibacterial activity of seed extracts from seven different mango cultivars (Mangifera indica L.), against MDR E. coli. The study focused on determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using dilution method. The concentrations examined for both MIC and MBC were 5, 10, 50, and 100 mg/mL. The results indicate that the mango seed extract exhibit significant antibacterial activity against MDR-E. coli., with the kweni cultivar showing the largest inhibition zone measuring 23.25 mm. The ethanol extract from the kweni seeds yielded the most favorable results, with an MIC value of ≥0.078 mg/mL and an MBC value that ranged from 1.25-10 mg/mL. In conclusion, mango seed extract, particularly from the kweni cultivar, has substantial potential to be developed as an effective antibacterial agent against MDR-E. coli.
Effect of subacute toxicity of mosquito coil smoke inhalation on lung histology and blood hematology parameters in Wistar rats Sri Rahayu, Mulyati; Wahyuni, Sri
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.6046

Abstract

Mosquito coils are made from incense products and are designed to repel insects using pyrethrins and pyrethroids as their main active ingredients. However, the use of these coils poses significant public health and ecological risks due to the emitted toxic fumes. These fumes can have long-term damaging effects on humans and the environment. This study aimed to investigate the effects of various durations of subacute exposure to mosquito coil smoke (MCS) on lung histology and blood hematology in white male Wistar rats (Rattus norvegicus). Rats were divided into four groups (n = 6): control; P1, P2, and P3, exposed to MCS for 5, 7, and 9 h per day, respectively, for 21 days. Hematological blood analyses (red cell count (RBC), hemoglobin (Hb), and hematocrit (Hct)) were performed, and lung histopathology was evaluated using Manja Roenigk's criteria. The group exposed to MCS for 9 h/day showed a significant decrease in mean body weight (185.00 ± 9.59) (p = 0.008) compared with the control group. All treated groups had normal Hb and Hct levels and RBC indices, with no significant differences compared with the control group. The 9-hour exposure group had significantly lower lung weights than the control group (p<0.05), and all treatment groups showed significant differences in lung weight (p<0.05). Histopathological analysis revealed significant loss of alveolar septal integrity, leading to alveolar lumen rupture and development of emphysema. This study provides clear evidence of emphysema due to subacute allethrin-based MCS inhalation, although it did not affect hematological parameters in rats.
Molecular identification and antibacterial activity of endophytic bacteria from Bambusa vulgaris leaves as antibacterial potential against phatogenic microoganism Malau, Jekmal; Mierza, Vriezka; Mulki, Munir Alinu; Urbaningrum , Lestari Mahardika; Hasna, Vina Luthfiana; Debora, Priscinya Christiana
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.6538

Abstract

The increasing prevalence of antibiotic-resistant bacteria poses a critical global health concern, necessitating the exploration of novel antibacterial solutions. Endophytic bacteria, which colonize plant tissues without causing harm, have gained attention as potential sources of bioactive metabolites. This study aimed to isolate and characterize endophytic bacteria from Bambusa vulgaris leaves and evaluate their antibacterial potential against pathogenic microorganisms. Leaf samples were collected from Bekasi, West Java, and subjected to a surface sterilization process prior to bacterial isolation. A total of 12 bacterial strains were successfully obtained and screened for antibacterial activity against Bacillus subtilis, Propionibacterium acnes, Pseudomonas aeruginosa, and Staphylococcus epidermidis using the agar well diffusion assay. Three isolates exhibited notable inhibitory activity, with P8 demonstrating the strongest antibacterial effects against B. subtilis, P. acnes, and S. epidermidis. The two most potent isolates, P8 and K3, were characterized via 16S rRNA gene sequencing. Genomic DNA extraction was performed, followed by Polymerase Chain Reaction (PCR) amplification using the universal primers 27F (5′-AGAGTTTGATYMTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′). Sequencing and The Basic Local Alignment Search Tool (BLAST) analysis confirmed that isolate P8 exhibited 100% similarity to B. subtilis strain LZH-H1, whereas isolate K3 shared 99.85% similarity with Pantoea stewartii subsp. indologenes strain SR2-12. These findings suggest that endophytic bacteria from B. vulgaris endohytic bacteria hold promise as potential sources of antibacterial compounds. Further research is necessary to purify and characterize these bioactive metabolites for potential pharmaceutical applications.
STAT3, but not Ki-67, expression is independently associated with tumor progression in colorectal carcinoma: A comparative immunohistochemical biomarker analysis Wedharga, I Gede Putu Adhi; Willianto, Vincentius Michael; Theodora, Imelda; Parengkuan, Irene; Novita, Bernadette Dian
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.7711

Abstract

The insidious progression of colorectal carcinoma (CRC) at early stages often hampers timely screening and diagnosis, resulting in delayed intervention and poor clinical outcomes. Ki-67 and Signal Transducer and Activator of Transcription 3 (STAT3) have been widely studied for their roles in tumor proliferation and survival. However, comparative studies assessing their clinicopathological relevance in CRC progression remain limited, and their relative contributions to tumor aggressiveness are unclear. This study aimed to evaluate the clinicopathological significance and association of Ki-67 and STAT3 expression with tumor aggressiveness, focusing on their roles in tumor infiltration and size as indicators of CRC progression. Thirty-eight formalin-fixed, paraffin-embedded (FFPE) CRC tissue specimens were collected from patients undergoing surgical resection for this cross-sectional study. Ki-67 and STAT3 expression were assessed using immunohistochemical (IHC) staining. Clinicopathological data were retrieved from medical records. Protein expression was quantified using inverted mean gray values via digital image analysis. Ki-67 and STAT3 expression levels significantly correlated with tumor infiltration (Ki-67: r = 0.322, p = 0.049; STAT3: r = 0.429, p = 0.007) and size (Ki-67: r = 0.425, p = 0.008; STAT3: r = 0.452, p = 0.004). No significant correlation was found between Ki-67 and STAT3 (r = 0.225, p = 0.175). Only STAT3 was independently associated with infiltration (OR per 1-standard deviation: 4.11, p = 0.043) and size (OR per 1-SD: 3.41, p = 0.047). While both markers are associated with CRC progression, only STAT3 shows an independent association with tumor aggressiveness, underscoring its role beyond cell proliferation and suggesting broader involvement in tumor invasion and progression pathways.
Acute oral toxicity and dose-dependent histopathological effects of Indonesian Ciplukan (Physalis angulata L.) extract in Sprague-Dawley rats Sylviningrum, Thianti; Inayati, Nor Sri; Setiawati, Setiawati; Arjadi, Fitranto; Novrial, Dody
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.7921

Abstract

The growing global interest in herbal medicines underscores the importance of safety assessments for traditional plants. Physalis angulata L. (Ciplukan) demonstrates therapeutic potential, yet data on Indonesian extracts remain limited. This study evaluated the acute oral toxicity and determined the median lethal dose (LD50) of Indonesian Ciplukan extract. Twenty-five female Sprague-Dawley rats were divided into five groups (n=5): control (aqueous), and four groups that received extract at 100, 400, 800, or 1200 mg/kg body weight. Over 14 days, researchers monitored clinical signs, body weight, food intake, and organ weights in accordance with Acute Oral Toxicity (OECD Test Guideline 425). Major organs were examined histopathological using the modified International Harmonization of Nomenclature and Diagnostic Criteria. No mortality or significant behavioral changes were observed at any dose. Rats maintained normal body weight gain and food intake. The LD50 was determined to be exceeded 1200 mg/kg, indicating favourable acute safety. However, histopathological analysis revealed significant dose-dependent necrotic changes (p < 0.05) in the lungs, kidneys, liver, and spleen at doses of 400 mg/kg and above. Liver and spleen damage were detected first at this threshold. These findings indicate an LD50 above 1200 mg/kg body weight for Indonesian Ciplukan extract, supporting its acute safety. Nonetheless, subclinical organ toxicity occurred at doses of 400 mg/kg or higher, emphasizing the need for dose optimization in phytomedicine. The study provides regulatory-grade toxicological data to support evidence-based standardization of Indonesian herbal medicines and highlights the necessity for further research on sub chronic and chronic toxicity to establish safe therapeutic doses.

Page 11 of 11 | Total Record : 105

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