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INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Published by Universitas Nahdlatul Ulama Surabaya
ISSN : 26846748     EISSN : 26569825     DOI : https://doi.org/10.33086/ijmlst
Core Subject : Health, Social,
Health Professions Social Sciences
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 97 Documents
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The comparison of the effectiveness of shoe flower (Hibiscus rosa-sinensis L.) and roselle flower (Hibiscus sabdariffa L.) infusions as alternative reagents for the examination of Soil Transmitted Helminths (STH) eggs Charisma, Acivrida Mega; Rahayu, Eka Octaviana; Anwari, Farida
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 1 (2024): Advancements in Biomedical Research: Insights from Medical Laboratory and Technol
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i1.5409

Abstract

Worm infections remain a significant health challenge globally, including in Indonesia, where they continue to contribute to a majority of health issues. Soil-transmitted helminths (STH), particularly intestinal nematode parasites, are predominant in causing these infections. Microscopic examination with 2% eosin staining is a conventional method for diagnosing worm infections. However, exploring alternative staining agents, such as anthocyanin-rich extracts from Hibiscus rosa-sinensis L. (hibiscus flower) and Hibiscus sabdariffa L. (roselle flower), presents a promising avenue. These plants possess anthocyanin compounds, resulting in a captivating red pigment and making them suitable for utilization as natural coloring agents. This study aimed to evaluate the efficacy of hibiscus and roselle infusions as alternative staining reagents for examining STH egg, compared to the standard 2% eosin staining method. Adopting a true experimental design, samples of STH worm egg suspensions were subjected to different concentrations of hibiscus and roselle infusions. The results revealed varying staining outcomes with different concentrations. However, a 1:1 concentration ratio of hibiscus and roselle infusions demonstrated effective staining against STH worm eggs. In conclusion, this research underscores the comparative effectiveness of hibiscus and roselle infusions as alternative staining reagents for STH egg examination, with 2% eosin serving as the control. These findings hold potential implications for improving diagnostic techniques in managing worm infections.
Thyroid dysfunction and semen quality among males investigated for infertility in Southern Nigeria Emokpae, Mathias Abiodun; Asiriuwa, Irabonosa; Osaikhuwuomwan, James Agbonmwanre
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 1 (2024): Advancements in Biomedical Research: Insights from Medical Laboratory and Technol
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i1.5664

Abstract

The relationship between thyroid and testis is well understood, and the association between changes in thyroid function and male infertility has been reported. However, the contribution of thyroid dysfunction to male infertility is not sufficiently addressed in our setting. This study aims to assess the thyroid hormones level among males undergoing investigation for infertility and to establish correlations between thyroid hormones and sperm indices. Thyroid hormones were determined in 150 infertile males and 50 fertile male controls. Semen analysis was done according to the World Health Organization criteria while thyroid hormones were determined using Enzyme linked Immunosorbent assay technique. The measured anthropometric data, sperm indices and thyroid hormone levels were compared using appropriate statistical tools. Serum triiodothyronine, and thyroxine levels were significantly lower (p < 0.001), while thyroid stimulating hormone was higher among infertile males than control subjects. The body mass index of the infertile subjects was significantly higher (p < 0.011) than control subjects. Of the 150 subjects, 41.33% (62/150) were euthyroid, 7.33% (11/150) had subclinical hypothyroidism while 51.34% (77/150) had overt hypothyroidism. Among the 88 altered thyroid function, 6.82% (6/88) had normozoospermia, 44.32% (39/88) had oligozoospermia while 48.86% (43/88) were azoospermia. The area Under the Curve of T3 (0.858), T4 (0.765) and sperm count (0.875) were able to differentiate fertile men from infertile subjects. Thyroid disorders are prevalent among infertile men and should be considered in the laboratory assessment of male infertility cases. Including thyroid function tests in the investigative panel can help identify and manage potential thyroid-related factors contributing to infertility. This comprehensive approach ensures thorough evaluation and targeted treatment for better reproductive outcomes in affected individuals.
Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 Soleha, Siti; Syarifah, Syarifah; Nurseha, Tito; Fatiqin, Awalul; Retnaningrum, Endah; Serang, Yitro
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5596

Abstract

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization of the Moraxella sp. SBE01 lipase coding gene is necessary for large-scale lipase production through genetic engineering. This study aimed to observe the molecular weight, amino acid sequence, length, and conserved amino acids in the DNA encoding the lipase gene, with the goal of identifying and characterizing the lipase-coding gene from Moraxella sp. SBE01. The primer design process was conducted to amplify the lipase gene from Moraxella sp. SBE01 using specialized software for sequence alignment and phylogenetic analysis. Amplification was carried out using PCR with the designed primer, forward primer (GTC ATG ATG TAC TTC CAY GGN GGN GG), reverse primer (GGT TGC CGC CGG CDS WRT CNC C). PCR was carried out under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles of denaturation at 95°C, annealing at 66°C (30 seconds), 70°C elongations (1 minute) and final elongation of 70°C (10 minutes). The PCR results were electrophoresed using 1% agarose gel with a 1 kb DNA marker. The PCR results were sequenced and analyzed for gene and amino acid sequences and the type of lipase expressed. Sequencing resulted in 387 bp of the nucleotide sequence. The gene and amino acid sequences from Moraxella sp. SBE01 had high homology with the gene and amino acid sequences from Moraxella sp. strain TA144. The lipase gene encodes a protein consisting of 129 amino acids and contains a conserved HGG (His-Gly-Gly) motif, which is characteristic of lipases in family IV, also known as the hormone-sensitive lipase (HSL) family. This conserved sequence suggests that the lipase shares structural and functional similarities with other enzymes in the HSL family, playing a key role in lipid metabolism.
Blumea balsamifera and Sargassum aquifolium extracts reduce fatty liver damage through lipid metabolism signalling pathways Widhiantara, I Gede; Wiradana, Putu Angga; Permatasari, Anak Agung Ayu Putri; Sari, Ni Kadek Yunita; Rosiana, I Wayan; Sandhika, I Made Gde Sudyadnyana; Panjaitan, Novaria Sari Dewi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5697

Abstract

Non-alcoholic fatty liver disease (NAFLD) is a condition marked by excessive fat accumulation in the liver and poses a significant health challenge. The leaves of Blumea balsamifera and Sargassum aquifolium have been reported to have anti-atherogenic effects. This study aims to determine the effectiveness of B. balsamifera extract (BBLE) and S. aquifolium extract (SAE) in preventing and treating liver fat accumulation in Wistar rats induced by a high-cholesterol diet through the expression of the AMP-activated protein kinase (AMPK)/ Sirtuin 1 (SIRT1)/peroxisome proliferator-activated receptor γ (PPARγ) pathway, and the leptin receptor. The experimental design of this study is laboratory-based, involving, 20 Wistar rats were fed a high-cholesterol diet over a period of 21 days. The rats were divided into four groups for the evaluation of BBLE and SAE effect: negative control (P0): induced with a high-cholesterol diet + distilled water, positive control (P1): induced with a high-cholesterol diet + simvastatin, P2: induced with a high-cholesterol diet + 4 mg/kg/bw BBLE, and P3: induced with a high-cholesterol diet + 4 mg/kg/bw BBLE and 4 mg/kg/bw SAE. The treatment duration extended over three months. Immunohistochemical analyses were performed on liver tissues to measure AMPK, SIRT1, PPARγ, and leptin receptor expression. The results indicated that leptin expression was lower in the BBLE+SAE group compared to the simvastatin group, and differences were significant between the BBLE and BBLE+SAE groups. No significant differences were noted in AMPK, SIRT1, and PPARγ expression between the simvastatin and BBLE+SAE groups (p≥0.05). In conclusion, BBLE and SAE effectively reduce liver lipid accumulation and enhance fat metabolism in hypercholesterolemic rats.
Immunomodulatory activity of kersen leaf extract (Muntingia calabura) on diabetic rats: analysis of immune response Hasan, Fonnie Esther; Yunus, Reni; Magdalena, Magdalena
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5746

Abstract

The immune response to high blood glucose levels leads to an inflammatory response and also produces inflammation mediators. Immunomodulatory functions of Kersen (Muntingia calabura) need further enhancement to ensure that its benefits are more widely recognized by the public. This study aims to determine the immunomodulatory activity of Kersen leaf in inducing and modulating the immune response in diabetic rats. This study was an experimental laboratory with a pre-and post-test with a control group design. The subjects were 30 white rats (Rattus Novergicus Wistar Strain), were treated with extract M. calabura dose 1 (100 mg/kg bw/day), dose 2 (200 mg/kg bw/day), dose 3 (300 mg/kg bw/day). For clinical evaluation, three control groups were formed, including a Normal Control Group, a Diabetes Mellitus (DM) Positive Group, and a DM Positive Group treated with Anti-Diabetic Drugs. The highest amount of IFN-γ concentrations were found in the DM positive control group + antidiabetic drugs (710.3 ± 27.2 ng/mL).  The highest number of Nitrit Oxide (NO) concentration was found in the DM positive control group (103.7 ± 10.2 µmol/L). The highest average amount of pancreatic β cell regeneration was found in the normal control group.  The DM positive control group and the treatment group had a significant difference (p < 0.05) It means that there is a significant difference in the data of all treatment groups, or these three groups have anti-diabetic activity by repairing or preventing damage to the pancreas organ in DM rats. This study revealed that M. calabura possesses immunomodulatory activity, capable of inducing and modulating immune responses in diabetic rats.
Ethanol crude leaf extract of weeping fig (Ficus benjamina) causes mild hepatotoxicity in Sprague dawley rats Udonkang, Mfoniso Iniobong; Douglas, Lynette Umasoye; Eworo, Raymond Ekong
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5798

Abstract

Weeping fig (Ficus benjaminaeaf extract has diverse medicinal properties but little is reported about its hepatotoxicity. This study determined the mean lethal dose (LD50) and investigated the effects of F. benjamina ethanol crude leaf extract on biochemical parameters and liver histology of Sprague Dawley rats. Twenty-nineemale rats weighing 133-204 g were used. The LD50 was determined with nine rats based on Lorke’s method. The experimental groups consisted of twenty rats, divided into four groups of five. Each group received treatment as follows: Control (feed and water only) and low, medium, and high doses (500, 1000, 1500 mg/kg respectively) of the extract orally for 21 days. All animals were weighed and sacrificed using Ketamine intra-peritoneal injection. Blood samples were collected for biochemical parameters of total bilirubin, conjugated bilirubin, aspartate aminotransferase (AST) alanine aminotransferase (ALT), and alkaline phosphatase (ALP). Liver tissues were removed and processed by the formalin-fixed-paraffin wax-embedding method. The tissue blocks were sectioned and subjected to Hematoxylin/Eosin and Masson trichrome staining. The extract’s LD50 was >5000 mg/kg. The rats’ body weights did not change statistically (p=0.985). Total bilirubin (p=0.003), conjugated bilirubin, AST, ALT, and ALP values (p=0.001) increased significantly. The AST high-doseroup had the highest fold increase (4.8). The liver histology showed mild sinusoidal dilation at 500 mg/kg. There was marked hemorrhage and fibrosis at medium and high doses. Although the extract had relatively low acute toxicity, 1000 and 1500 mg/kg doses were associated with mild hepatotoxicity characterized by veno-occlusion disease. The 500 mg/kg dose is safer for medicinal purposes.
Prevalence and comorbid for late-stage chronic kidney disease (CKD) in patients undergoing continuous ambulatory peritoneal dialysis (CAPD) due to urinary obstruction Hanafi, Hafidz; Daryanto, Besut; Gunawan, Atma
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5821

Abstract

Chronic Kidney Disease (CKD) is a condition of gradual or chronic decline in kidney function, which is quite severe and caused by various kidney diseases, including urinary obstruction. This disease is progressive and generally irreversible. CKD requires kidney replacement therapy, one of which is continuous ambulatory peritoneal dialysis (CAPD). To determine the prevalence and risk factors for End Stage Renal Disease (ESRD) in patients undergoing CAPD due to urinary obstruction. We performed a retrospective cohort with a cross-sectional study was conducted using secondary data from medical record data of ESRD patients with CAPD accompanied by urinary obstruction at Dr. Saiful Anwar General Hospital, Malang, Indonesia. The prevalence of CKD in patients with CAPD accompanied by urinary obstruction was 6,50% and dominated by males (57,8%) with an age range of 41-50 years (26%). The majority of comorbidities are severely high the Body Mass Index (BMI) (89,0%) and hypertension (80,8%). The location of obstruction is mostly unilateral (5,64%) with mild levels (4,06%). Urinary obstruction is a frequent clinical finding in CKD patients with CAPD. The most common risk factor in this study was hypertension. The prevalence and comorbidities among CAPD patients with Urinary obstruction (UO) are better understood because to this study. It is necessary to recognise its limitations, particularly the small sample size and single-centre design. Future studies should involve more centres and larger patient groups in order to provide a more thorough knowledge of the mechanisms behind the high survival rates among CAPD patients.
Analysis of bone mineral profile and TSH in early non-dialysis stages of chronic kidney disease - a retrospective cross-sectional study Rajan, Immaculate Rithika; Elango, Karthick; Selvarajan, Sathya
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5864

Abstract

The objective of this study is to analyse the levels of Thyroid stimulating hormone (TSH) and the Bone Mineral Profile (Vitamin D, Parathyroid hormone (PTH), calcium, phosphorus, magnesium, and alkaline phosphatase [ALP]) levels in the early stages of chronic kidney disease (CKD) based on Glomerular filtration rate (GFR). A retrospective analysis was conducted involving 247 CKD patients admitted to the nephrology department at Sri Ramachandra Medical College Hospital from January to June 2022. The estimated GFR (eGFR) was calculated utilizing the CKD-EPI formula provided by the National Kidney Foundation. All biomarkers were analysed using automated platforms. The baseline ages for the three groups were 52.5, 68, and 66.5 years respectively (p<0.001). The comparative analysis revealed statistically significant differences solely among Vitamin D, creatinine, PTH, and phosphorus across the three groups. Further correlation analysis demonstrated changes in bore significant correlations with only creatinine, vitamin D, and PTH. This study concludes that in the early stages of CKD, vitamin D followed by PTH appears to be the earliest biomarker for assessing CKD-Mineral and Bone Disorder (CKD-MBD) occurring prior to any alterations in calcium and phosphate levels. As such, early consideration of supplementation may prove beneficial in mitigating disease progression and preventing cardiovascular complications.
Expression of gonadotropin-releasing hormone receptor type-II correlates with proliferation activity in tissue microarray of rare ovarian tumor Ariesaka, Kiky Martha; Kusumanto, Ardhanu; Zucha, Muhammad Ary; Anggorowati, Nungki; Wicaksono, Agil Wahyu; Yunus, Moch; Fanani, Erianto; Nuryady, Moh Mirza
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.6034

Abstract

Activation of Gonadotropin-Releasing Hormone type II Receptors (GnRHR-II) exhibits antiproliferative activity. GnRHR-II is not only expressed exclusively in the pituitary, but also in a variety of tumors. To date, the clinical relevance of GnRHR-II in ovarian tumors is unclear. In addition, there is a lack of literature addressing GnRHR-II in ovarian tumors, especially rare types. This study was conducted to investigare the correlation between GnRHR-II expression with clinicopathology and proliferative activity of rare ovarian tumors. The purpose of this study was an analytic observational study with a cross-sectional design that utilized 18 ovarian tumor samples on tissue microarray (TMA). The expression of GnRHR-II and Ki67 was assessed using immunohistochemical staining (IHC) and observed using the IHC profiler plugin ImageJ software to obtain their respective H-scores. The data was analyzed using independent t-test, ANOVA, Pearson's test, and Fisher's exact test based on data types. The value of p<0.05 was considered statistically significant. GnRHR-II is expressed in various forms in ovarian tumors, including extrapituitary expression. GnRHR-II expression was highest in the sex cord stromal tumor (SCST) group, 110.30 ± 23.89 (p<0.0001). In addition, there was also a significant difference between GnRHR-II expression with age (p<0.001) and the primary tumor (p<0.05), but not with tumor type (p=0.101). There is a correlation between GnRHR-II expression and proliferative activity (r=-0.043, p=0.866). Elevated GnRHR-II expression is significantly correlation with SCST, individuals over 40 years of age, and tumors confined to the ovary and it is correlates with lower proliferative activity, although this correlation is very weak.
Spectroscopic characterization of rosella flower extract (Hibiscus sabdariffa L.) and its antibacterial activity against Enterobacter aerogenes in suspected typhoid cases Rohani, Siti; Martha, Fadhil Rizki; Mundijo, Trisnawati; Yesi Astri; Zilda, Malika; Melianti, Agnes
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.5577

Abstract

Typhoid, a serious bacterial disease, has spurred research into natural products like rosella (Hibiscus sabdariffa L.) for potential treatments. This study investigates the chemical components found in Rosella extract using spectroscopy approximation, assisted by Fourier transform infrared spectroscopy (FTIR) and Gas chromatography–mass spectrometry (GC–MS). The antibacterial activity of rosella extract on bacteria from stool cultures of suspected typhoid cases continues to be evaluated. The antibacterial experimental employed a post-test-only control group design, using 30 μg Chloramphenicol as a positive control, sterile distilled water as the negative control, rosella extract at concentrations of 25%, 50%, 75%, and 100% as the observed variable. Stool samples from typhoid patients were identified, and Enterobacter aerogenes were detected using VITEK®2 testing. Cultivated bacteria from the samples were tested to determine the antibacterial activity of the rosella extract. Phytochemical studies confirmed the presence of tannins, alkaloids, flavonoids, and saponins in the rosella extract. Additionally, the spectroscopic evaluation from FTIR and GC-MS showed the presence of chemical groups, including esters, aldehydes, and aromatics. Further clinical tests demonstrated antibacterial activity at the minimum inhibitory concentration. The results showed an increasing inhibition zone of bacterial growth, correlating with the increase in rosella extract concentration. Although the antibacterial activity of rosella extract was lower compared to commercial Chloramphenicol, this natural product has demonstrated antibacterial activity and shows potential as a candidate for future herbal medicine development.

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