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INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Published by Universitas Nahdlatul Ulama Surabaya
ISSN : 26846748     EISSN : 26569825     DOI : https://doi.org/10.33086/ijmlst
Core Subject : Health, Social,
Health Professions Social Sciences
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 105 Documents
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Betatrophin: A promising biomarker for metabolic syndrome and diabetes mellitus risk screening in teenagers Susanto, Hendra; Aulanni’am, Aulanni’am; Wuragil, Dyah Kinasih; Taufiq, Ahmad; Sunaryono, Sunaryono; Trijoyo Purnomo, Jerry Dwi; Krisnawati, Dyah Ika; Sholeh, Moch
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6028

Abstract

Metabolic syndrome (MetS) and diabetes mellitus (DM) have become primary concerns worldwide, especially among the younger population. The Indonesian Boarding School model (IBS/Boarding School) is a large education system with a significant number of pupils (teenagers) and has the potential to become a center for metabolic disease, particularly among teenagers, due to their daily intake. This study aimed to provide a baseline screening for MetS and the risk of DM development in Boarding School teenagers. During this observational study, 90 healthy serological samples were obtained from senior and junior high school students. The circulating level of betatrophin was measured using a human betatrophin ELISA kit. Additionally, metabolic syndrome and DM screening data were analyzed using a rapid reverse-flow immunochromatography kit for 65 kDa glutamic acid decarboxylase (GAD65). Among the 90 healthy students, a high prevalence of GAD65 was observed, indicating a potential risk factor for metabolic diseases. Furthermore, higher serum betatrophin levels were observed in the samples. The circulating level of betatrophin was found to have a significant correlation with age, gender, body mass index (BMI), systolic blood pressure (SBP), fasting blood glucose (FBG), sleeping duration, and duration of stay at Boarding School (p < 0.05). Betatrophin emerged as a potential predictor of BMI, SBP, and FBG in students (p < 0.05). Both betatrophin and GAD65 have shown promise as future biomarkers, opening up a new avenue for assessing metabolic syndrome and the risk of DM. This underscores the importance of future programs in Boarding Schools focusing on MetS and DM prevention management, making the audience feel the significance of their work in addressing these pressing health issues.
The clinical value of systemic immune-inflammation index (SII) and prognostic nutritional index (PNI) for predicting the occurrence of metastasis in patients with lung cancer Biutifasari, Verna; Hardjono, Ellen Angelique; Adiwinoto, Ronald Pratama; Hadi, Janto Poernomo
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.5628

Abstract

Systemic immune-inflammation index (SII) and prognostic nutritional index (PNI) serve as simple and practical tests that help indicate inflammatory and nutritional status to some extent. Lung cancer stands out as the most common contributor to cancer-related mortality globally. It is associated with an unfavorable prognosis. Most patients diagnosed with lung cancer have metastasis at the time of diagnosis. Prognostic heterogeneity of cancer patients causes the need for more prognostic biomarkers. This study aimed to evaluate the clinical value of SII and PNI in predicting metastasis in lung cancer patients. SII and PNI provides a prognostic value in lung cancer. Retrospective cross-sectional research was conducted in this study involving 138 data from medical records at the Inpatient and Outpatient Department of Pulmonology, RSPAL dr. Ramelan Surabaya in April 2019 to July 2023. Kolmogorov-Smirnov test, contingency coefficient test, and ROC analysis were done to analyze the data obtained. Patients with metastatic lung cancer had higher SII than those without metastasis. The group of patients with metastasis had an average SII of 5391.34 and a PNI of 40.11. The group of patients without metastasis had an average SII of 2849.52 and PNI of 43.05. Lung cancer metastasis was correlated significantly with SII but not with PNI. The cut-off value was determined using the ROC curve. The cut-off value for SII was 2198.54 (68.5% sensitivity and 58.7% specificity) and for PNI was 42.2 (62% sensitivity and 54.3% specificity). SII was correlated with lung cancer metastasis and may be a promising indicator predicting of metastasis. PNI showed no significant correlation with lung cancer metastasis.
In vitro anticoagulant activity of crude fibrinolytic protease HSFT-2 as an alternative anticoagulant agent Afriansyah, Muhammad Ardi; Rahajoe, Toeti; Pranindira, Raksi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.5758

Abstract

Thrombotic disorders are one of the leading causes of death in cardiovascular patients and contribute to the incidence of non-infectious diseases. The search for new anticoagulant agents to overcome the limitations associated with existing anticoagulant therapies is increasing, especially those derived from natural and microbial sources. Staphylococcus hominis HSFT-2 is a Gram-positive coccus isolated from rusip Holothutia scabra commonly known as sand sea cucumber. Fibrinolytic protease HSFT-2 has been reported to be able to degrading fibrin, which has shown several characteristics of fibrinolytic and clot-lysis activities. This study aims to evaluate the anticoagulant activity of crude fibrinolytic protease HSFT-2 in vitro as potential alternative anticoagulant agents. The research design was completely randomized, with a sample size of 21 participants calculated using the Federer formula. The material used was crude protease derived from S. hominis cultured in Skim Milk Broth. Various tests, including blood routine tests, platelet aggregation tests, and blood smear evaluations, were conducted to assess its effectiveness as an anticoagulant. Whole blood samples were divided into three groups: a positive control, a negative control, and a group treated directly with 100 µL of crude fibrinolytic protease. The normality of the data was evaluated using the Kruskal-Wallis test, while differences between groups were analyzed with one-way ANOVA. The one-way ANOVA results from the blood routine tests indicated a significant difference between the control and the fibrinolytic protease HSFT-2 (p<0.05). The platelet aggregation percentage decreased from 75.85% in the control group to 21.75% with the addition of crude protease HSFT-2. Furthermore, the blood smear evaluations revealed no abnormalities in cell formation when HSFT-2 protease was used, compared to samples without the addition of EDTA anticoagulant. In conclusion, the fibrinolytic protease HSFT-2 exhibits anticoagulant activity; however, it does not yet meet the standards required for use as an anticoagulant in routine blood tests. Future work should focus on the purification and characterization of the protease to enhance its anticoagulant potential for laboratory diagnostic applications.
Significance of serum protein electrophoresis in diagnosing multiple myeloma: A retrospective study at a tertiary care centre Sundar, Shrinidhi; Prasad , Swetha Raghavendra; Kumar, Dhivya Senthil; Selvarajan, Sathya; Krishnamurthy, Sowmya; Sathyamurthy, Sridharan Kopula
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6712

Abstract

Multiple myeloma (MM) is a hematologic malignancy characterized by uncontrolled plasma cell proliferation in the bone marrow, initially asymptomatic, often causing symptoms like bone pain, anemia, renal dysfunction, and increased infection susceptibility. The study investigates the clinical significance of inconsistencies in albumin measurement methods in multiple myeloma patients, focusing on serum protein electrophoresis (SPEP) and the bromocresol green (BCG) assay. A comparative analysis was conducted to evaluate the differences between albumin levels measured by SPEP and BCG, exploring how these variations relate to disease severity and treatment decisions in MM patients. According to the results, SPEP albumin levels were significantly higher than BCG albumin levels in MM patients (p <0.001). This difference is clinically significant, as lower BCG albumin readings could overestimate disease severity, potentially leading to misclassification and affecting treatment decisions. Accurate albumin measurement is crucial for proper staging and prognosis in multiple myeloma. In conclusion, this highlights the need to integrate SPEP and biochemical markers for more precise diagnosis and timely intervention. While SPEP is essential for diagnosing MM and monitoring M-protein (also known as monoclonal immunoglobulin or paraprotein), the BCG method remains useful for staging and prognosis. Standardizing laboratory protocols and exploring novel biomarkers could improve MM diagnosis, ensuring more accurate disease classification and better patient management. Further research is needed to assess the long-term impact of these discrepancies on treatment outcomes and survival rates.
Exploration and molecular identification of proteolytic bacteria from rusip pacific oyster (Crassostrea gigas) as anticoagulant agent candidates Afriansyah, Muhammad Ardi; Sudarwin; Dewi, Sri Sinto; Akbar, Gusti Dimas Refian
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6083

Abstract

The marine symbiont Staphylococcus epidermidis strain CGF-6, a protease-producing bacterium, has been successfully isolated from Rusip Pacific Oyster (Crassostrea gigas). S. epidermidis is a non-spore-forming, Gram-positive coccus commonly found in marine environments due to their ability to tolerate high salinity. The aim of this study was to identify proteolytic bacteria from Rusip fermented C. gigas as potential candidates for the development of anticoagulant agents. Bacterial isolation was performed through the fermentation process of Rusip. After seven days, bacterial colonies were purified three times using Nutrient Agar. The selection of proteolytic bacterial was conducted qualitatively using a skim milk agar medium. The bacterial isolates exhibiting the highest protease activity were identified through 16S rRNA gene sequencing using universal primers Bact 27F and UniB 1492R. Phylogenetic tree analysis, conducted with the MEGA X program, helped determine the relationships between species. Out of the 18 bacterial isolates obtained from the Rusip fermentation of C. gigas, three isolates (CGF-1, CGF-6, and CGF-11) exhibited hydrolysis zones around their colonies on skim milk agar, indicating protease activity.  Among these, isolate CGF-6 showed the highest proteolytic index of 0.5 and was identified as Staphylococcus epidermidis strain CGF-6. S. epidermidis strain CGF-6 has the potential to serve as a valuable source of protease production for the development of anticoagulant agents. However, further studies, including enzyme characterisation, optimisation, and both in vitro and in vivo anticoagulant activity tests, are necessary to assess the efficacy and safety of this enzyme as a candidate for anticoagulant agents.
Quality assessment and biochemical characterization of probiotic Lactobacillus species isolated from yogurts fermented either by Tamarindus indica or Capsicum annuum Thiagarajan, Sowmiya; Bhaskar, Emmanuel; Gayathri, Veeraraghavan; Mohanalakshmi, Parthasarathy; Silambanan, Santhi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6280

Abstract

Yogurt is used to treat various digestive ailments. However, there are limited studies of the biochemical and microbiological properties of yogurt. This study aimed to assess the quality and biochemical characteristics of probiotic Lactobacillus species isolated from yogurts fermented with Tamarindus indica or Capsicum annuum. Three yogurt samples were used for analysis; two were homemade, using either Tamarindus indica L. or Capsicum annuum L. to initiate fermentation. Third was a commercial yogurt obtained from the grocery market. A proximate analysis was carried out. To identify microbiological strains, yogurt samples were cultured on nutrient- and De Man-Rogosa-Sharpe agars. Biochemical characteristics were examined, and probiotic species were identified through 16S rRNA analysis. The results showed that traditional yogurts had lower energy content due to reduced carbohydrate and fat levels while exhibiting higher protein content. Escherichia coli and coliforms were detected in the commercial yogurt, whereas traditional yogurts were free from these harmful bacteria, highlighting their potential safety. The biochemical characteristics of all yogurt samples were largely similar. C. annuum L. demonstrated a positive oxidase test, indicating the presence of an electron transport chain in the Lactobacillus species found in the yogurt fermented with C. annuum L. Microbial analysis revealed that L. acidophilus was predominant in traditional yogurt, while commercial yogurt contained L. plantarum. These findings suggest that homemade yogurts, with their higher protein content, lower fat and carbohydrate levels, and absence of harmful bacteria, offer a safer and potentially more beneficial probiotic alternative to commercial yogurt. The specific Lactobacillus strains present in traditional yogurts may contribute to their antimicrobial properties, supporting their probiotic potential.
Vitamin D enhances migration but decreases gene expression of vascular endothelial growth factor and tumor necrosis factor-α in Wharton’s jelly mesenchymal stem cells Yuhendri, Vitriyanna Mutiara; Pratiwi, Nurfi; Paulina, Ariza Julia; Ismawati, Ismawati; Saputra, Nicko Pisceski Kusika; Arfianti, Arfianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6698

Abstract

Mesenchymal stem cells (MSCs) have gained significant interest for their potential application in repairing damaged tissues through immunomodulatory functions. Vitamin D has been shown to regulate both the innate and adaptive immune systems. Active forms of Vitamin D, such as 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) are known to influence the action of mesenchymal stem cells (MSCs) in several mechanisms, such as stimulating their proliferation and osteogenic differentiation, as well as tissue regeneration. This study aimed to investigate the effects of vitamin D supplementation on the biological properties and expression of cytokines and growth factor genes in MSCs isolated from the human umbilical cord (UC). MSCs were isolated from Wharton’s jelly (WJ-MSC) of UC, cultured, and supplemented with various concentrations of vitamin D3. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability, and a scratch wound healing assay was conducted to evaluate the migration capacity of MSCs. The mRNA expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were quantified using reverse transcription-polymerase chain reaction (RT-PCR). This study showed that supplementation with 50 nM of vitamin D3 for 48 h significantly increased the viability and migratory capacity of MSCs. Furthermore, vitamin D supplementation significantly decreased the mRNA levels of TNF-α and VEGF but did not affect IL-6 gene expression compared to the control group. These findings suggest that vitamin D supplementation can enhance the biological characteristics and modulate the expression of key immunomodulatory factors in MSCs, potentially improving the effectiveness of MSC-based therapies.
Assessement of Candida albicans of the oral mucosa among type-2 diabetes mellitus patients attending Bahteramas Hospital, Southeast Sulawesi Province Firdayanti, Firdayanti; Fusvita, Angriani; Umar, Ani
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes Mellitus (DM) is a disease characterized by hyperglycemia resulting from either insulin deficiency or insulin resistance. Candida albicans is a fungus generally found on the surface of the mucous membrane, which usually dominates and overgrows among DM patients. This study aims to identify the presence of the C. albicans fungus on the oral mucosa of individuals with Type-2 Diabetes Mellitus at Bahteramas Hospital, Southeast Sulawesi Province, Indonesia. A descriptive study was employed, and a purposive sampling technique was used to select a sample of 62 patients. Swab samples were cultured on Potato Dextrose Agar medium for Gram staining and carbohydrate assimilation tests. The resulting culture was tested using CHROMagarTM Candida (CAC) media. Among the 62 samples, 56 samples were identified to have a convex colony, a cream-color surface, a smooth texture, and a Gram-positive nature. The carbohydrate assimilation test showed that the indicated samples could ferment glucose and maltose, but not lactose and sucrose. Green colonies are formed through CHROMagarTM Candida differential media testing. There are 56 identification results for C. albicans from oral mucosal swab samples from 62 patients with Type-2 Diabetes Mellitus at the Bahteramas Hospital, Southeast Sulawesi Province, yielding six other patients with negative results.
In vivo effect of bromelain from Ananas comosus on Staphylococcus aureus skin infection in an animal model Zul, Siti Nur Atikah; Wahab, Mohd Shazwan Shazdee; Ismail, Elysha Nur; Ishak, Reezal
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.5739

Abstract

The skin is the largest organ in the body and aids in protection against foreign microorganisms. Any physical changes to the skin structure would eventually promote microbial colonization that leads to soft tissue and skin infections. This study was conducted to establish the effectiveness of bromelain extraction from Ananas comosus in treating Staphylococcus aureus skin infections in BALB/c mice. A total of 18 male mice aged 9 - 12 weeks were divided into three groups: bromelain-treated, iodine-treated, and negative control groups. The skin infection was inflicted on the dorsal area of the mouse by first removing the hair and applying the tape-stripping method before inoculating it with S. aureus. The microbial samples from the infected skins of the mice were collected on days 1, 4, and 7 after infection using a Q-Swab apparatus and streaked on blood agar. The samples were incubated at 37°C for 24 h and the S. aureus colony formation unit (CFU) from each group was calculated. The results indicated that mice treated with bromelain had a lower average number of S. aureus CFU (2.55x10-2 CFU/mL) compared with iodine-treated mice (1.56x10-2 CFU/mL) and had a significantly different count compared with the negative control group (8.24x10-2 CFU/ml) on day 7 post-infection. Data were analyzed using one-way ANOVA and Student’s t-test for significant differences with p<0.05. In conclusion, the findings of this study showed that bromelain can be developed and applied as an antimicrobial agent against S. aureus skin infection.
In silico approach of antiviral compound from Cymbopongon citratus as the main protease SARS-CoV-2 inhibitor Arianto, Steven
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.5754

Abstract

A pandemic known as COVID-19 is brought on by the RNA virus SARS-CoV-2. SARS-CoV-2 has some protease enzymes, such as main proteases (Mpro), that regulate the expression of viral non-structural proteins. The Mpro protein is a possible target for SARS-CoV-2 antiviral therapy because the inhibition of Mpro will disrupt the virus replication process and stop the virus life cycle. Lemongrass is an Indonesian spice with bioactive compounds whose benefits have not been widely explored, especially as an antiviral for SARS-CoV-2. This study aimed to identify potential bioactive chemicals in lemongrass that have antiviral properties for treating COVID-19. This methodology research began by preparing the bioactive compounds of lemongrass and Mpro protein. Using Lipinski’s rule of five, the nine bioactive chemicals found in lemongrass were examined to see how similar they were as therapeutic compounds. Next, PyRx 0.8 (Virtual Screening Tool) and PyMOL were used to perform molecular docking and its interaction with the Mpro protein. Diethyl phthalate has antiviral and anti-inflammatory activity with low binding energy (-5.8 kcal/mol) for Mpro and is stably bound (RMSF < 3Å) via hydrophobic bonds (His41, Met165) and hydrogen bonds (Phe140, His163, His164, Glu166, Gln189). It is concluded that diethyl phthalate on lemongrass demonstrated high promise as an inhibitor of SARS-CoV-2 viral replication based on the findings of the comprehensive investigation of these bioactive chemicals.

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