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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 13 Documents
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Search results for , issue "Vol 15, No 3 (2009)" : 13 Documents clear
PERBANDINGAN ANTARA KADAR KALIUM SERUM DENGAN ATAU TANPA TERAPI INSULIN PADA PASIEN DIABETES MELLITUS TIPE 2 Andi Syamsudduha; S. V Sembiring; R DN Pakasi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.972

Abstract

Diabetic patients can suffer from hipokalemia because of prolonged osmotic diuretic and management diabetic mellitus with insulinif does not associate with kalium intake. The aimed of this study was to evaluate kalium serum level on type 2 DM, and comparisonof kalium serum level on type 2 DM patients with or without insulin therapy. A cross-sectional study was done on 33 patiens type 2diabetic mellitus that admission on Stella Maris Hospital who examined kalium serum level. The data were colected from Medical Recordperiode January to December 2007. Data were analyzed with Fisher Exact test using SPSS versi 11.5. From 33 samples found normalkalium level 9 samples (27.3%) and decreased 6 samples (18.2%) and without insulin therapy found decresed 3 samples (9.1%) andnormal Kalium level 15 samples (45.5%). Comparison of Kalium serum level of type 2 DM patiens with or without insulin therapydid not statistically significant with P = 0.135. Kalium serum level of type 2 DM patiens with or without insulin therapy commonly inreference value.
KADAR PENERIMA TRANSFERIN TERLARUT (sTFR) DI PENDERITA HIV/AIDS DENGAN ANEMIA Indrati AR; Van Crevel R; Sumantri R; Wisaksana R
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.963

Abstract

Anemia is the most common hematologic abnormality associated with HIV which affecting 60 to 80 percent of patients in the latestage of the disease. The presence of anemia is associated with increased of morbidity and mortality in patients with HIV infection. Irondeficiency, chronic inflammation and antiretroviral treatment (ACT) may cause HIV associated anemia. The differentiation of irondeficiency anemia from chronic disease anemia is a diagnostic challenge. Maybe it is helpful in soluble transferrin receptor (sTfR), thecleaving of the extra cellular domain related to transferrin receptor. Because the elevated sTfR concentration is a marker of tissue irondeficiency and increased marrow erythropoietin activity. The aim of this study was to examine the diagnostic value of soluble transferrinreceptor level in anemia patients with HIV/AIDS. The Study was the part of the IMPACT (Integrated Management for Prevention, Controland Treatment of HIV/AIDS) baseline and cohort study. The study started since September 2007 in RSUP Hasan Sadikin Bandung.There were 179 HIV/AIDS patients with anemia included in this study. Complete blood count, reticulocytes, feritin, soluble transferringreceptor and hsCRP were tested in these patients. It was found that the mean of sTfR in HIV patients with anemia were 1238.42U/mL(304.5-30435). sTfR had a low correlation with MCV (r -0.174), feritin (r -0.65) and absolute reticulocyte counting (r 0.172). Feritinhad moderate and significant correlation with hsCRP (r:0.429; p 0.00). There was no significant difference of sTfR level between thepatients without ART, with Zidovudin and d4T (p 0.81). There was no significance difference of sTfR concentration between the low andnormal MCV level (p 0.341). sTfR can not differentiate the source of anemia in patients with HIV/AIDS. It can be concluded so far thatchronic disease and inflammation as reflected by the elevated hsCRP level and use of zidovudine are the main cause of anemia.
TAMPANG JENUH TRANSFERIN PENDONOR DARAH ANEMIA Christina Roosarjani; Titis Wahyuono; J B Suparyatmo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.977

Abstract

Iron deficiency remains one of the most frequent adverse effects of blood donation. Iron status test used on blood donor screeningis haemoglobin concentration. Other iron status parameters are transferrin saturation. The study aims to determine the profile oftransferrin saturation among certain groups of blood donors at the Blood Transfusion Unit of the Indonesian Red Cross SurakartaBranch. The samples were drawn from blood donors at the Blood Transfusion Unit from June to December 2005. A total of 148 specimenswere classified into 3 groups consist of 49 first time blood donations as group I, 50 of fifth time blood donations as group II, and 49of tenth time blood donations as group III. Transferrin saturation was measured by ratio between serum iron and Total Iron BindingCapacity (TIBC). The data analysed by Anova test to distinguish the difference of transferrin saturation among three groups. The resultsshowed the transferrin saturation decreased from group I to group II and from group II to group III. The transferrin saturation amongthree groups showed significantly difference (p=0.000). It can be concluded that there is a decrease in transferrin saturation accordingto the blood donation frequency among blood donors at the Blood Transfusion Unit of the Indonesian Red Cross Surakarta Branch.Transferrin saturation measurement is needed for another parameter of iron deficiency anaemia among blood donors.
BERBAGAI KESALAHAN TATA LANGKAH PEKERJAAN LABORATORIUM KLINIK Prihatini Prihatini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.968

Abstract

Laboratory procedure errors occurred in some cycle parts during prae-analytic, analytic and post analytic sessions, they must beexcluded and prevented in the process because the outcome would support the diagnosis of diseases also to enhance their confidence.The laboratory works were carried out in the right methods, is suppervised accurately base on haematological, microbiological, clinicalchemistry and immunological standard examinations. Any deviation although the procedure results according the standard one mustbe registered. Laboratory errors revealed must be monitored as the means to reduce errors and to threat them well. Beside it is necessaryto have a good communication between patients, doctors as well as with the laboratory services.
PENERIMA ASAM RETINOID a (a RETINOID ACID RECEPTOR) DI LEUKEMIA AKUT PROMYELOSITIK DENGAN BATANGAN (ROD) AUER Adi K Aman; Tonny .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.966

Abstract

Acute Promyelocytic Leukemia (APL) is a subtype of AML with a defined clinical course and biology that is distinct from otherforms of AML. The term M3-AML was assigned to the hyper granular promyelocytic leukaemia that is characterized by blast cells withazurophilic granules, bundles of auer rods and reniform or bilobed nucleus. Clinically, APL is related to disseminate intravascularcoagulation and abnormal fibrinolysis. Cytogenetically, APL may cause translocation on the promyelocytic leukaemia (PML) gene,and chromosome 15 and with the retinoic acid receptor α (RARA) gene, on chromosome 17. The diagnosis of APL is shown by bonemarrow morphologically The majority of cells in the bone marrow are abnormal, having some similarities with promyelocytes. Themalignant cells bear numerous large granules and several auer rods. Aspirates of bone marrow are also taken for cytogenesis evaluationand for detecting the translocation. A twelve years girl was admitted to the hospital with haemorrhage from the gums during 5 dayafter extraction of a tooth. On physical examination there is no organomegaly shown. The laboratory examination found normocyticnormochromic anaemia, leucopoenia and thrombocytopenia with 15% blast cell and 5% promyelocytes with multiple auer rods. Thebone marrow aspirates showed predominant of promyelocyte cells (70%) with multiple auer rods.
ELEKTROFORESIS PROTEIN SERUM PASIEN DENGAN KADAR PROTEIN NORMAL Tiene Rostini; Coriejati Rita
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.962

Abstract

Serum protein electrophoresis pattern can assist in diagnosis of liver disease, hematological disorders, renal disorders andgastrointestinal disease. Measurement of total protein level in the serum cannot detect any disorders in patient with normal limit ofserum total protein level. The aim of this study; was to evaluate the serum protein electrophoresis pattern in patient with normal limitsof serum protein level. This research was carried out by descriptive retrospective study using the electrophoresis data from patients’medical record at the Clinical Pathology Department, Dr. Hasan Sadikin General Hospital Bandung. The data of serum electrophoresis (bySebia gel electrophoresis) were grouped based on disease or disorders, and confirmed with the diagnosis derived from patient’s medicalrecord. Inclusion criteria of samples if ; the electrophoresis data were available, serum total protein level within normal limits (6.4–8.3mg/dL), and the data of electrophoresis taken from medical record were taken from August 2006 until August 2008. The result foundso far was, there were 240 data of electrophoresis from patients with serum protein level within normal limits (6.4–8.3 mg/dL). theinterpretation of electrophoresis consist of: 1) inflammation (149 patients; 62.2% ; sensitivity 83.7%, specificity 86,5%) 2) Cirrhosis(46 patients ; 19.2% ; sensitivity 87.5% ; specificity 88.4%) 3) Nephritic syndrome (15 patients ; 6.2%; sensitivity 53%; specificity96.9% 4) Monoclonal gammophaty (15 patients(6.2% ; sensitivity 80% ; specificity 98.7%) 5) Normal pattern in 15 patient (6.2%).This study found abnormal serum protein electrophoresis pattern in the condition of inflammation, Cirrhosis, Nephritic Syndrome, andMonoclonal gammophaty. It can be concluded that many disorders could be detected in patient with serum protein level within normallimits such as: inflammation, cirrhosis, nephritis syndrome and monoclonal gammophaty by abnormal electrophoresis pattern
ANALISIS TES IMUNOKROMATOGRAFI DAN ENZYME-LINKED IMMUNOSORBENT ASSAY UNTUK MENDETEKSI Helicobacter pylori DI PASIEN DISPEPSIA I Hutagalung; Uleng Bahrun; Mansyur Arif; Rifai Amirudin; HAM Akil
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.959

Abstract

Many methods are available to diagnose Helicobacter pylori in patients with dyspepsia, including Enzyme-Linked ImmunosorbantAssay (ELISA) and recently used is immnochromatography test. Both of the test are non invasive method, but immunochromatographytest can be used in laboratory without semi automatic analyzer. The aim of this study was to compare the immunochromatographyand ELISA tests in detecting the possibility of Helicobacter pylori infection. A cross sectional study was done among 49 sampleswith dyspepsia at Wahidin Sudirohusodo Hospital of Makassar and Prodia Laboratory from March to August 2008. Samples wereanalyzed with SPSS 14 for Windows Program using Chi Square and Spearman correlation tests. Among 49 samples we found ELISAand immunochromatography tests were positive in 5 samples, ELISA and immunochromatography tests were negative in 33 samples,ELISA tests were borderline and immunochromatography tests were positive in 5 samples, and ELISA tests were borderline andimmunochromatography tests were negative in 6 samples. There was a good correlation between ELISA and immunochromatographytest with R = 1.000 and p = 0.000. Both results of the immunochromatography and ELISA tests showed high conformity. Both methodcan be applied to diagnose Helicobacter pylori in patients with dyspepsia. Immunochromatography test can be an alternative methodin laboratories who do not apply semi automatic analyzer. The advantages of immunochromatography test can be used for diagnoseearly infection of Helicobacter pylori.
PERHITUNGAN JUMLAH SEL CD4 DENGAN SEROPOSITIF IgM HERPES SIMPLEKS TIPE-2 DI PASIEN HIV Bastiana Bermawi; Endang Retnowati K; Erwin A Triyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.970

Abstract

Herpes simplex type-2 (HSV-2) infection is usually transmitted sexually and can cause recurrent, painful genital ulcers. Seropositivityof IgM HSV-2 indicates acute infection of HSV-2. The low level of CD4 cell counts can lead to opportunistic infection in HIV patients.Therefore, the aim of this study is to investigate the association between CD4 cell counts and HSV-2 infection (based on seropositivity ofIgM HSV-2) in HIV patients with heterosexual risk factor in Dr. Soetomo Hospital, Surabaya. Specimens were collected during the firstpresentation on June and July 2008. HSV-2 IgM and was assessed by an ELISA technique using Herpelisa 2 IgM (recombinant) reagent,whereas CD4 cell counts was assessed by a Flowcytometry BD FACSCaliburTM System. The seropositivity of IgM HSV-2 in HIV persons18 years of age or older was 27.3% (9/33). Median CD4 cell counts the number were 50 cells in μl. Persons with seropositive HSV-2who had CD4 cell counts < 200 cells/μL and > 200 cells/μL were 66.6% (6/9) and 33.3% (3/9), respectively. Using chi square test,there was no association between CD4 cell counts and seropositivity of IgM HSV-2 in HIV patients. The seropositivity of IgM HSV-2 inHIV patients with heterosexual risk factor in Dr. Soetomo Hospital was 27.3%. There was no association between CD4 cell counts andthe seropositivity of IgM HSV-2 in HIV patients with heterosexual risk factor.
ANEMIA KEKURANGAN (DEFISIENSI) ZAT BESI BAYI Aida Amelda; Hanifah Maani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.961

Abstract

Anemia usually happened in childhood. Babies 6–12 years old as are those group that are sensitive to iron deficiency anemia (IDA)Hemoglobin is a mean corpuscular volume (MCV), red-cell distribution width (RDW) examination with blood film evaluation that canbe used to screen IDA, especially in high risk babies. This study was carried out by the cross sectional method in healthy babies 6-12years old in Padang city, on November 2006 until January 2007. They were examined in the Clinical Pathology Laboratory of Dr. MDjamil hospital Padang. The samples consisted of 2 ml venous blood EDTA and laboratory examinations. The results were Hb, MCV,and RDW with ABX micros 60. Also blood film evaluation if Hb were 11g/dL. The means of Hb, MCV and RDW were 11.1 ± 1.07 g/dL,71.7 ± 4.70 fl and 14. 2 ± 1.37%. In 62 (45.9%) babies were anemic as a result of IDA examination and the rest of 33 babies (532%) the blood film were normocytic normochromic 49% and normocytic hypochromic (+), mild anisocytosis 51%. In Padang city theIDA examination in 6-12 years old babies were high frequencies in grade I and II by blood film method
PERBANDINGAN KADAR HEMOGLOBIN ANTARA METODE SPECTROPHOTOMETER DENGAN METODE HEMOCUE PADA SAMPEL LEUKOSITOSIS Basti Andriyoko; Leni Lismayanti; Delita Prihatni
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.975

Abstract

Background and objective: The measurement of haemoglobin was carried out by using hematology analyzer with specthrophotometermethod which measured light absorbance at 540 nm. However, this measurement affected by increase turbidity as a result of leucocytosis.The turbidity can be eliminated by using HemoCue method that detect absorbance at 570 nm and 880 nm. The aim of this study wasto compare the measurement of haemoglobin obtained between specthrophotometer method and HemoCue method. Materials andmethod: Blood EDTA sample that have been measured with MEK-6318K Nihon Kohden hematology analyzer for haemoglobin levelswith spectrophotometer methods with leucocyte > 100.000/mm3 were included in this study. Blood sample are measured again forthe haemoglobin level with HemoCue B-Haemoglobin Analyzer. This study was conducted at Clinical Pathology Laboratory, Dr. HasanSadikin Hospital Bandung from August–October 2008. Result: Seventeen leucocytosis sample were enroled in this study. The meanhaemoglobin level from specthrophotometer method is higher than HemoCue method, however there was no statistically significantdifference between haemoglobin result from specthrophotometer method and HemoCue method (p = 0.742 > a = 0.05). Conclusion:There was no significant difference beetween specthrophotometer method and HemoCue method for haemoglobin measurement ofleucocytosis sample.

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