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Tiene Rostini, Tiene
Departemen Patologi Klinik, Fakultas Kedokteran Universitas Padjadjaran/ Rumah Sakit Umum Pusat Dokter Hasan Sadikin Bandung
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Efek Ekstrak Etanol Daun Gynura procumbens dalam Menghambat Peningkatan Kadar Trigliserida Tikus Model Hipertrigliseridemia Astuti, Yunita Puji; Utami, Novi Vicahyani; Rostini, Tiene
Acta Pharmaceutica Indonesia Vol 39, No 1 & 2 (2014)
Publisher : School of Pharmacy Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.206 KB)

Abstract

Hipertrigliseridemia penting diperhatikan karena merupakan faktor resiko independen penyakit diabetes melitus. Salah satu tanaman obat yang dipercaya masyarakat dapat menurunkan kadar lipid adalah tanaman sambung nyawa (Gynura procumbens). Penelitian eksperimental dilakukan untuk mengetahui efek ekstrak etanol daun sambung nyawa dalam menghambat peningkatan kadar trigliserida darah tikus model hipertrigliseridemia. Tikus jantan galur Wistar sebanyak 24 ekor, umur 2-3 bulan, berat 200-300 gram, dibagi 6 kelompok: kelompok I (makanan standar + air), kelompok II (diet tinggi lemak [DTL] + propiltiourasil [PTU] 0,01%+ larutan carboxymetyl cellulosa 1%), kelompok III (DTL+ PTU 0,01%+ ekstrak 50 mg/kgBB), kelompok IV (DTL+ PTU 0,01%+ ekstrak 150 mg/kgBB), kelompok V (DTL + PTU 0,01% + ekstrak 300 mg/kgBB) dan kelompok VI (DTL + PTU 0,01%+ gemfibrozil 54 mg/kgBB). Kadar trigliserida diukur setelah satu minggu adaptasi (sebelum perlakuan) dan setelah dua minggu perlakuan. Data hasil penelitian dianalisis dengan uji Kruskal-Wallis dan Mann-Whitney. Hasil penelitian menunjukkan bahwa ekstrak etanol daun sambung nyawa dosis 50 mg/kgBB, 150 mg/kgBB dan 300 mg/kgBB memiliki efek menghambat peningkatan kadar trigliserida darah tikus model hipertrigliseridemia (p<0,05). Ketiga dosis memiliki efek yang sebanding. Ekstrak etanol daun sambung nyawa menghasilkan efek yang sebanding dengan gemfibrozil dosis 54 mg/kgBB (p>0,05). Kesimpulan penelitian ini adalah ekstrak etanol daun sambung nyawa memiliki efek menghambat peningkatan kadar trigliserida darah tikus model hipertrigliseridemia. Efek tersebut sebanding dengan efek gemfibrozil 54 mg/kgBB.Kata kunci: Gynura procumbens, sambung nyawa, ekstrak, trigliserida, hipertrigliseridemiaAbstractHypertriglyceridemia is an important condition as risk factor for diabetes mellitus, which shows an increasing prevalence annualy. People believed that herbs such Gynura procumbens act as herbal medicine for hypertriglyceridemia. The experimental study was conducted to know the effect of Gynura procumbens ethanolic extract in inhibiting the increase of blood triglyceride level of hypertriglyceridemic rats model. Twenty four male Wistar rats aged 2-3 months (200-300 grams), were divided into 6 groups: group I (standard food + water), group II (high lipid diet + propylthiouracil 0,01% + carboxymetil celullosa solvent 1%), group III (high lipid diet + propylthiouracil 0.01% + extract 50 mg/kg), group IV (high lipid diet + propylthiouracil 0.01% + extract 150 mg/kg), group V (high lipid diet + propylthiouracil 0.01% + extract 300 mg/kg), and group VI (high lipid diet + propylthiouracil 0.01% + gemfibrozil 54 mg/kg). The triglyceride level was measured: one week after adaptation (before treatment) and two weeks after treatment. The data was analyzed using Kruskal-Wallis test and Mann-Whitney test. The results of the study indicated that Gynura procumbens ethanolic extract of 50 mg/kg, 150 mg/kg, and 300 mg/kg had effect in inhibiting the increase of the blood triglyceride level of hypertriglyceridemic rats model (p<0,05). The variation of the three doses indicated a similar effect. Gynura procumbens ethanolic extract and gemfibrozil 54 mg/kg gave a similar effect (p>0,05). In conclusion, Gynura procumbens ethanolic extract has an effect in inhibiting the increase of blood triglyceride level of hypertriglyceridemic rats model. It has similar effect to gemfibrozil 54 mg/kg.Keywords: Gynura procumbens, extract, triglyceride, hypertriglyceridemia
Incidence of Neonatal Hyperbilirubinemia based on Their Characteristics at Dr. Hasan Sadikin General Hospital Bandung Indonesia Nurani, Namira Bening; Kadi, Fiva Aprillia; Rostini, Tiene
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.874 KB)

Abstract

Background: Hyperbilirubinemia is the most frequent clinical condition that occurs in neonates identified by yellow discoloration of the skin and other tissues. It is caused by elevations of bilirubin level as the effect of increased breakdown of red blood cells, impaired conjugation of indirect bilirubin, and incomplete bilirubin excretion. This study was conducted to discribe the characteristics of neonates with hyperbilirubinemia.Methods: This study used descriptive quantitative method which took retrospective data from medical record of neonates with hiperbilirubinemia in 2014. The population of this study was neonates which were born in the perinatology division at Dr. Hasan Sadikin General Hospital, Bandung. The variables collected were gender, gestational age, weight birth, general condition ad treatment options. The collected data were tabulated and presented by percentage and in the form of a table.Results: One hundred and seventy four of 230 medical records of neonates with hyperbilirubinemia were collected based on the inclusion criteria. The results showed that 8.04% of 2,531 neonates born at Dr. Hasan Sadikin General Hospital in 2014 suffered from hyperbilirubinemia. Males (56.9%) were higher than females to have hyperbilirubinemia. Neonates born at term gestational age (55.2%) were more likely to have hyperbilirubinemia with low birth weight category (51.7%), healthy general condition (60.4%). The most treatment option was  phototherapy (54.6%).Conclusions: Most neonates with hyperbilirubinemia are males, who were born at aterm gestational age, with low birth weight. Most of the neonates are in healthy general condition and the most common treatment given to neonates is phototherapy.
Hyperuricemia and Proteinuria in Type 2 Diabetic Patients Haryandini, Fatya Annisa; Rostini, Tiene
Althea Medical Journal Vol 2, No 1 (2015)
Publisher : Althea Medical Journal

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Abstract

   Background: Type 2 diabetes mellitus is a metabolic disorder that has diabetic nephropathy as a complication characterized by proteinuria. When type 2 diabetic patient’s kidney is damaged, hyperuricemia occurs. This study evaluated the correlation of serum uric acid and proteinuria levels in type 2 diabetic patients from outpatient Endocrine clinic at Dr. Hasan Sadikin General Hospital from January to December 2011.Methods: In a cross-sectional study of 125 patients (55 male and 70 female) with type 2 diabetes mellitus, the data of serum uric acid, fasting plasma glucose, 2-h post oral glucose load plasma glucose, serum high-density lipoprotein cholesterol, serum trygliceride, and proteinuria levels were accessed from the medical record. Spearman test was used to calculate the correlation of serum uric acid and proteinuria levels. The research used secondary data taken from medical records  of patients diagnosed with T2DM  from outpatient Endocrine clinic of Internal Medicine Department at Dr. Hasan Sadikin General Hospital Bandung who had alaboratory examination at Department of Clinical Pathology of Dr. Hasan Sadikin General Hospital Bandung from January to December 2011.Results: The mean age of the type 2 diabetic patients was 61.5±9.2 years old, with mean serum uric acid level of 6.2±1,8 mg/dl, of whom 52.8% had microalbuminuric and 4.8% had proteinuria. In type 2 diabetic patients, serum uric acid level correlated positively (r=0.273) with proteinuria (p=0.002).Conclusions: Higher serum uric acid levels associated significantly positive with a greater probability of proteinuria in type 2 diabetic patients, indicating a potential marker for disease severity.Keywords: Proteinuria, type 2 diabetes mellitus, uric acidDOI: 10.15850/amj.v2n1.430 
CORRELATION BETWEEN SERUM MID-REGIONAL PRO-ADRENOMEDULLIN AND SEQUENTIAL ORGAN FAILURE ASSESSMENT (SOFA) SCORE IN PATIENT WITH SEPSIS Pujiyanti, Hapsari; Lismayanti, Leni; Rostini, Tiene; Parwati, Ida
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.557 KB) | DOI: 10.15395/mkb.v51n2.1456

Abstract

Most sepsis will develop into multi organ failure (MOF). To assess manifestation of MOF, SOFA score that includes several laboratory parameters for each organ is used. However, this requires time and is also costly. Recently, Mid-Regional Pro-Adrenomedullin (MR proADM) biomarkers are stated to be an alternative marker of MOF in sepsis because MR proADM is secreted by endothelials that may increase in sepsis or bacterial infection. The aim of this study was to analyze the correlation between serum MR proADM levels and SOFA score. This was a cross-sectional  observational analytical study conducted in Dr. Hasan Sadikin General Hospital (RSHS) Bandung from August 2017 to July 2018. This study was a part of the bigger sepsis biomarker study. Samples used in this study consisted of 50 stored serum from the Sepsis Biomarker study in which the MR proADM was measured. Analysis using Spearman?s correlation test showed a moderate positive correlation between serum MR proADM level and SOFA score (r = 0,582, p=0.000), showing that MR proADM serum was directly proportional to SOFA score. It is concluded that MR proADM can be considered as one of the biomarkers for multi organ failure.Korelasi Kadar Mid Regional ProAdrenomedullin Serum dengan Skor Sequential Organ Failure Assessment (SOFA) pada Penderita SepsisSebagian besar sepsis akan berlanjut menjadi multi organ failure (MOF). Untuk mengetahui MOF digunakan skor SOFA yang memerlukan beberapa parameter laboratorium untuk tiap-tiap organ dan membutuhkan waktu dengan biaya yang mahal. Saat ini diketahui biomarker Mid Regional proAdrenomedullin (MR proADM) dapat digunakan sebagai biomarker MOF pada sepsis karena MR proADM disekresi oleh endotel yang akan meningkat pada keadaan sepsis atau infeksi bakteri. Tujuan penelitian ini mengetahui korelasi kadar MR proADM serum dengan skor SOFA. Penelitian ini bersifat observasional analitik dengan rancangan potong lintang dilaksanakan di RSUP Dr. Hasan Sadikin (RSHS) Bandung pada bulan Agustus 2017 sampai Juli 2018. Penelitian ini bagian dari payung penelitian ?Biomarker pada sepsis?. Bahan pemeriksaan berupa bahan biologis tersimpan sebanyak 50 sampel dan dilakukan pemeriksaan MR proADM. Hasil analisis menggunakan Spearman?s correlation test menunjukkan korelasi positif sedang antara kadar MR proADM serum dan skor SOFA (r=0,582; p=0,000). Hal ini menunjukkan semakin tinggi kadar MR proADM serum maka semakin tinggi skor SOFA. Simpulan, terdapat korelasi positif sedang dan bermakna antara MR proADM dengan skor SOFA, sehingga MR proADM dapat dipertimbangkan sebagai salah satu biomarker pada multi organ failure.
Kadar Asam Urat Serum sebagai Biomarker Preeklamsi Sumanti, Neli; Noormartany, -; Alamsyah, Muhammad; Rostini, Tiene
Majalah Kedokteran Bandung Vol 45, No 2 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Preeklamsi masih merupakan masalah kesehatan yang menjadi salah satu penyebab kematian ibu selain akibat perdarahan dan infeksi. Etiologi dan patogenesis preeklamsi belum diketahui pasti. Peningkatan kadar asam urat serum timbul bersamaan dengan peningkatan tekanan darah dan terjadi sebelum timbul proteinuria sehingga dapat dipakai sebagai salah satu biomarker. Tujuan penelitian ini menganalisis perbandingan kadar asam urat serum pada kehamilan in partu normal dengan in partu preeklamsi. Penelitian potong lintang dilakukan di Rumah Sakit Dr. Hasan Sadikin Bandung pada bulan Maret–Mei 2011. Subjek penelitian 45 wanita hamil in partu normal sebagai kelompok kontrol dan 44 wanita in partu dengan preeklamsi sesuai dengan kriteria inklusi dan eksklusi. Kadar asam urat serum pada wanita hamil normal adalah 3,43±0,14 mg/dL. Pada penelitian ini nilai titik potong kadar asam urat serum 4,8 mg/dL dengan nilai sensitivitas 93% dan spesifisitas 80%. Simpulan,kadar asam urat pada in partu preeklamsi lebih tinggi dibandingkan dengan kehamilan normal. Peningkatan kadar asam urat dapat dipertimbangkan sebagai salah satu biomarker preeklamsi, sebaiknya kadar asam urat serum digunakan sebagai pemeriksaan serial pada ibu hamil saat pelayanan antenatal. [MKB. 2013;45(2):98–104]Kata kunci: Biomarker, kadar asam urat, preeklamsiSerum Levels of Uric Acid as a Biomarker in PreeclampsiaPreeclampsia remains a health problem that becomes one of the causes of maternal deaths besides bleeding and infection. The etiology and pathogenesis of preeclampsia are unclear. Increased serum uric acid levels is seen simultaneously with the increase of blood pressure and occurred before the onset of proteinuria. Therefore, the uric acid can be used as a biomarker. The aim of this study was to analyze the serum uric acid levels between normal and preeclampsia pregnancies. The study was conducted in Dr.Hasan Sadikin Hospital Bandung between March and May 2011, using cross sectional study design. Subjects were 45 inpartu normal pregnant women as control and 44 in partu pregnant women with preeclampsia accordance with inclusion and exclusion criteria. Levels of uric acid in normal pregnancy are 3,43 ±0.14 mg/dL. In this study uric acid levels resulting in cut-off levels of 4,8 mg/dL with a sensitivity value of 93%, and specificity 80%. Conclusions: uric acid levels in at term preeclampsia are higher compared with normal pregnancies. Increased levels of uric acid can be considered as one of biomarkers of preeclampsia, hence the serum uric acid levels used as serial examinations in pregnant women during antenatal care. [MKB. 2013;45(2):98–104]Key words: Biomarker, preeclampsia, uric acid level DOI: http://dx.doi.org/10.15395/mkb.v45n2.89
KORELASI JUMLAH FOLIKEL ANTRAL DENGAN KADAR 25(OH)D SERUM PADA PENDERITA SINDROM OVARIUM POLIKISTIK Mustari, Asri Dini; Rostini, Tiene; Indrati, Agnes Rengga; Bayuaji, Hartanto; Rachmayati, Sylvia
Majalah Kedokteran Bandung Vol 50, No 4 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (624.066 KB) | DOI: 10.15395/mkb.v50n4.1469

Abstract

Sindrom ovarium polikistik (SOPK) ditandai oleh hiperandrogenisme, oligo atau anovulasi, dan morfologi ovarium polikistik. Penanda ovarium polikistik, yaitu terdapat ?12 folikel antral dengan diameter 2?9 mm pada pemeriksaan ultrasonografi. Vitamin D (25-hidroksivitamin D; 25(OH)D) berperan pada proses steroidogenesis di sel teka ovarium dan pengaturan ekspresi reseptor follicle stimulating hormone (FSH) di sel granulosa ovarium. Defisiensi 25(OH)D menyebabkan penurunan aktivitas FSH, sehingga folikel antral di ovarium berukuran kecil dan berjumlah banyak. Tujuan penelitian ini mengetahui korelasi jumlah folikel antral dengan kadar 25(OH)D serum pada penderita SOPK. Uji observasional analitik  dengan rancangan potong lintang di Rumah Sakit Umum Pusat Dr. Hasan Sadikin (RSHS) Bandung dilakukan selama bulan September 2017?Juni 2018. Subjek penelitian adalah 52 penderita SOPK yang telah didiagnosis oleh klinisi obstetri dan ginekologi RSHS Bandung. Pengambilan darah vena dilakukan untuk pemeriksaan kadar 25(OH)D serum dengan metode ELISA. Hasil penelitian ini mendapatkan 56% subjek memiliki kadar 25(OH)D serum ?20 ng/mL. Hasil uji korelasi Spearman didapatkan korelasi negatif kuat yang bermakna (r = -0,867, p<0,001) jumlah folikel antral dengan kadar 25(OH)D serum. Kadar 25(OH)D serum yang rendah pada penderita SOPK berkorelasi dengan jumlah folikel antral yang banyak. Terdapat korelasi negatif antara jumlah folikel antral dan kadar 25(OH)D serum pada penderita SOPK.  Kata kunci: Folikel antral, 25(OH)D serum, sindrom ovarium polikistik  Correlation between Antral Follicles Count and Serum 25(OH)D Levels in Polycystic Ovary Syndrome PatientsPolycystic ovary syndrome (PCOS) is characterized by hyperandrogenism, oligo- or anovulation, and polycystic ovarian morphology. Vitamin D (25-hydroxyvitamin D; 25(OH)D) plays a role in the steroidogenesis process in the ovarian theca cells and regulates follicle stimulating hormone (FSH) receptor expression in granulosa cells. In 25(OH)D deficiency there is a decrease in FSH activity that the follicles become small and the antral follicles count in the ovary increases. The aim of this study was to determine the relationship between antral follicles count and serum 25(OH)D levels in PCOS patients. A cross-sectional analytical observational study was conducted in Dr. Hasan Sadikin General Hospital Bandung in September 2017?June 2018. Subjects of this study were 52 PCOS patients diagnosed by obstetricians and gynecologists of the hospital. Blood vein samples were collected to examine serum 25(OH)D levels using ELISA method. Serum 25(OH)D level of ?20 ng/mL was found in 55.8% of the subjects in this study. Analysis using Spearman?s correlation test showed a significant negative correlation (r = -0.867, p <0.001) between antral follicles count and serum 25(OH)D level in patients with PCOS. Lower levels of 25(OH)D serum in PCOS patients correlates with higher number of antral follicles count. There is a negative correlation between antral follicles count and serum 25(OH) D level in PCOS patients.Key words: Antral follicle count, 25(OH)D serum levels, polycystic ovary syndrome 
Incidence of Neonatal Hyperbilirubinemia based on Their Characteristics at Dr. Hasan Sadikin General Hospital Bandung Indonesia Namira Bening Nurani; Fiva Aprillia Kadi; Tiene Rostini
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.874 KB)

Abstract

Background: Hyperbilirubinemia is the most frequent clinical condition that occurs in neonates identified by yellow discoloration of the skin and other tissues. It is caused by elevations of bilirubin level as the effect of increased breakdown of red blood cells, impaired conjugation of indirect bilirubin, and incomplete bilirubin excretion. This study was conducted to discribe the characteristics of neonates with hyperbilirubinemia.Methods: This study used descriptive quantitative method which took retrospective data from medical record of neonates with hiperbilirubinemia in 2014. The population of this study was neonates which were born in the perinatology division at Dr. Hasan Sadikin General Hospital, Bandung. The variables collected were gender, gestational age, weight birth, general condition ad treatment options. The collected data were tabulated and presented by percentage and in the form of a table.Results: One hundred and seventy four of 230 medical records of neonates with hyperbilirubinemia were collected based on the inclusion criteria. The results showed that 8.04% of 2,531 neonates born at Dr. Hasan Sadikin General Hospital in 2014 suffered from hyperbilirubinemia. Males (56.9%) were higher than females to have hyperbilirubinemia. Neonates born at term gestational age (55.2%) were more likely to have hyperbilirubinemia with low birth weight category (51.7%), healthy general condition (60.4%). The most treatment option was  phototherapy (54.6%).Conclusions: Most neonates with hyperbilirubinemia are males, who were born at aterm gestational age, with low birth weight. Most of the neonates are in healthy general condition and the most common treatment given to neonates is phototherapy.
Hyperuricemia and Proteinuria in Type 2 Diabetic Patients Fatya Annisa Haryandini; Tiene Rostini
Althea Medical Journal Vol 2, No 1 (2015)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.522 KB)

Abstract

   Background: Type 2 diabetes mellitus is a metabolic disorder that has diabetic nephropathy as a complication characterized by proteinuria. When type 2 diabetic patient’s kidney is damaged, hyperuricemia occurs. This study evaluated the correlation of serum uric acid and proteinuria levels in type 2 diabetic patients from outpatient Endocrine clinic at Dr. Hasan Sadikin General Hospital from January to December 2011.Methods: In a cross-sectional study of 125 patients (55 male and 70 female) with type 2 diabetes mellitus, the data of serum uric acid, fasting plasma glucose, 2-h post oral glucose load plasma glucose, serum high-density lipoprotein cholesterol, serum trygliceride, and proteinuria levels were accessed from the medical record. Spearman test was used to calculate the correlation of serum uric acid and proteinuria levels. The research used secondary data taken from medical records  of patients diagnosed with T2DM  from outpatient Endocrine clinic of Internal Medicine Department at Dr. Hasan Sadikin General Hospital Bandung who had alaboratory examination at Department of Clinical Pathology of Dr. Hasan Sadikin General Hospital Bandung from January to December 2011.Results: The mean age of the type 2 diabetic patients was 61.5±9.2 years old, with mean serum uric acid level of 6.2±1,8 mg/dl, of whom 52.8% had microalbuminuric and 4.8% had proteinuria. In type 2 diabetic patients, serum uric acid level correlated positively (r=0.273) with proteinuria (p=0.002).Conclusions: Higher serum uric acid levels associated significantly positive with a greater probability of proteinuria in type 2 diabetic patients, indicating a potential marker for disease severity.Keywords: Proteinuria, type 2 diabetes mellitus, uric acidDOI: 10.15850/amj.v2n1.430 
Solanum muricatum Aiton Juice as A Hepatoprotective Agent in Wistar Rats Induced With Carbon Tetrachloride Justine Sim Wei Yang; Istriati Istriati; Tiene Rostini
Althea Medical Journal Vol 2, No 4 (2015)
Publisher : Faculty of Medicine Universitas Padjadjaran

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Abstract

Background: Liver participates in various metabolic processes in human body. Exposures to toxins such as carbon tetrachloride (CCl4) results in hepatocyte destruction and release the cell contents. Enzymes such as serum glutamic pyruvic transaminase (SGPT) were used as a parameter to diagnose liver damage. Pepino (Solanum muricatum Aiton) contains antioxidants that protect liver from hepatoxicity. The aim of this experiment is to determine the effect of pepino in protecting the hepatocyte from hepatotoxic effect of CCl4.Methods: A total of 16 Wistar rats used as the subject were divided into Control and Therapeutic Group. The Control group was induced with CCl4 but was not given pepino juice. Meanwhile, the Therapeutic Group was given pepino juice for 10 days. For induction of hepatotoxicity, CCl4 10% was given at a dosage of 2.0 ml/kg intra-peritoneal. Each Wistar rat in Therapeutic Group was given 1.0 ml of 300 g/ml pepino juice via oral feeding. Spectrophotometer with adjusted wavelength of 340 nm was used to measure SGPT level of the Wistar rats and statistical T-test was used to analyze the data.Results: Wistar rats in Therapeutic group showed a larger decrease (6898.9 IU/L–79.1 IU/L) in SGPT level compared to the Control group (6469.8 IU/L–418.5 IU/L). The SGPT level of the Wistar rat in Therapeutic group reached normal baseline (50 IU/L–150 IU/L). The significance of the experiment was supported by the T-test, P-value <0.05Discussion: Pepino juice had antioxidants that protect the hepatocyte of Wistar rats from free radicals. The synergistic action of antioxidants and hepatocyte regeneration of Wistar rats in Therapeutic group caused a decrease in SGPT level. Therefore, the experiment concluded that pepino juice at the dosage of 300 g/ml has hepatoprotective effect.Key words: Carbon tetrachloride, hepatotoxicity, Pepino, Solanum muricatum Aiton, SGPT DOI: 10.15850/amj.v2n4.484
Efek Ekstrak Etanol Daun Gynura procumbens dalam Menghambat Peningkatan Kadar Trigliserida Tikus Model Hipertrigliseridemia Yunita Puji Astuti; Novi Vicahyani Utami; Tiene Rostini
Acta Pharmaceutica Indonesia Vol. 39 No. 1 & 2 (2014)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Abstract

Hipertrigliseridemia penting diperhatikan karena merupakan faktor resiko independen penyakit diabetes melitus. Salah satu tanaman obat yang dipercaya masyarakat dapat menurunkan kadar lipid adalah tanaman sambung nyawa (Gynura procumbens). Penelitian eksperimental dilakukan untuk mengetahui efek ekstrak etanol daun sambung nyawa dalam menghambat peningkatan kadar trigliserida darah tikus model hipertrigliseridemia. Tikus jantan galur Wistar sebanyak 24 ekor, umur 2-3 bulan, berat 200-300 gram, dibagi 6 kelompok: kelompok I (makanan standar + air), kelompok II (diet tinggi lemak [DTL] + propiltiourasil [PTU] 0,01%+ larutan carboxymetyl cellulosa 1%), kelompok III (DTL+ PTU 0,01%+ ekstrak 50 mg/kgBB), kelompok IV (DTL+ PTU 0,01%+ ekstrak 150 mg/kgBB), kelompok V (DTL + PTU 0,01% + ekstrak 300 mg/kgBB) dan kelompok VI (DTL + PTU 0,01%+ gemfibrozil 54 mg/kgBB). Kadar trigliserida diukur setelah satu minggu adaptasi (sebelum perlakuan) dan setelah dua minggu perlakuan. Data hasil penelitian dianalisis dengan uji Kruskal-Wallis dan Mann-Whitney. Hasil penelitian menunjukkan bahwa ekstrak etanol daun sambung nyawa dosis 50 mg/kgBB, 150 mg/kgBB dan 300 mg/kgBB memiliki efek menghambat peningkatan kadar trigliserida darah tikus model hipertrigliseridemia (p<0,05). Ketiga dosis memiliki efek yang sebanding. Ekstrak etanol daun sambung nyawa menghasilkan efek yang sebanding dengan gemfibrozil dosis 54 mg/kgBB (p>0,05). Kesimpulan penelitian ini adalah ekstrak etanol daun sambung nyawa memiliki efek menghambat peningkatan kadar trigliserida darah tikus model hipertrigliseridemia. Efek tersebut sebanding dengan efek gemfibrozil 54 mg/kgBB.Kata kunci: Gynura procumbens, sambung nyawa, ekstrak, trigliserida, hipertrigliseridemiaAbstractHypertriglyceridemia is an important condition as risk factor for diabetes mellitus, which shows an increasing prevalence annualy. People believed that herbs such Gynura procumbens act as herbal medicine for hypertriglyceridemia. The experimental study was conducted to know the effect of Gynura procumbens ethanolic extract in inhibiting the increase of blood triglyceride level of hypertriglyceridemic rats model. Twenty four male Wistar rats aged 2-3 months (200-300 grams), were divided into 6 groups: group I (standard food + water), group II (high lipid diet + propylthiouracil 0,01% + carboxymetil celullosa solvent 1%), group III (high lipid diet + propylthiouracil 0.01% + extract 50 mg/kg), group IV (high lipid diet + propylthiouracil 0.01% + extract 150 mg/kg), group V (high lipid diet + propylthiouracil 0.01% + extract 300 mg/kg), and group VI (high lipid diet + propylthiouracil 0.01% + gemfibrozil 54 mg/kg). The triglyceride level was measured: one week after adaptation (before treatment) and two weeks after treatment. The data was analyzed using Kruskal-Wallis test and Mann-Whitney test. The results of the study indicated that Gynura procumbens ethanolic extract of 50 mg/kg, 150 mg/kg, and 300 mg/kg had effect in inhibiting the increase of the blood triglyceride level of hypertriglyceridemic rats model (p<0,05). The variation of the three doses indicated a similar effect. Gynura procumbens ethanolic extract and gemfibrozil 54 mg/kg gave a similar effect (p>0,05). In conclusion, Gynura procumbens ethanolic extract has an effect in inhibiting the increase of blood triglyceride level of hypertriglyceridemic rats model. It has similar effect to gemfibrozil 54 mg/kg.Keywords: Gynura procumbens, extract, triglyceride, hypertriglyceridemia
Co-Authors Agnes Rengga Indrati Agnes Rengga Indrati Anna Tjandrawati Arto Yuwono Astuti, Yunita Puji Coriejati Rita David Rustandi Delita Prihatni Dewi Kartika Turbawaty Dewi Nurhayati Fatya Annisa Haryandini Fatya Annisa Haryandini, Fatya Annisa Fiva Aprillia Kadi Hartanto Bayuaji Ida Parwati Istriati Istriati Jane Haryanto Justine Sim Wei Yang Kadi, Fiva Aprillia Khairunnissa, Khairunnissa Kurnia Pramono, Laila Leni Lismayanti Leni Lismayanti Lisda Amalia Muhammad Alamsyah Mulya Harahap, Raja Iqbal Mustari, Asri Dini Namira Bening Nurani neli sumanti Nida Suraya Nida Suraya Nina Tristina Noormartany Novi Vicahyani Utami Nurani, Namira Bening Pujiyanti, Hapsari Rahmawati Nurmin Raja Iqbal Mulya Harahap Rita Rachmayanti Rizki Andriyani Ryan Bayusantika Sylvia Rachmayati Towifah Fauziah Choerunisa Yunita Puji Astuti