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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 12 Documents
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Search results for , issue "Vol 16, No 2 (2010)" : 12 Documents clear
SD DENGUE DUO® (NS1, IgG, IgM) RAPID TEST DALAM MENUNJANG DIAGNOSIS INFEKSI VIRUS DENGUE Diah Puspita Rini; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.980

Abstract

Dengue virus infection can cause dengue hemorrhagic fever (DHF) which is still a major health problem in Indonesia. Thediagnosis of DHF is established based on WHO criteria and IgM/IgG antidengue serologic markers. A rapid method is needed for thedetecting or screening the disease. Antigen detection (NS1) can be performed by immunochromatography (rapid test) or enzyme -linkedimmunosorbent assay (ELISA). Recently, a rapid test to detect NS1 as an antigen and IgM/IgG antidengue to differentiate primary andsecondary dengue virus infection is available in one cassette. This study evaluated the new commercial dengue rapid test, SD DengueDuo for the detection of both antigen and antibodies to dengue virus. Serum samples used in this study were collected from 33 denguevirus infection patients according to WHO criteria and admitted in the Tropical Ward, Dr. Soetomo Hospital. Samples were taken twice,during acute and convalescent phase. SD Dengue Duo (NS1, IgG, IgM) rapid test was used and confirmed by ELISA as the gold standard.To determine the diagnostic specificity 20 samples of non dengue virus infection (typhoid fever and malaria) confirmed by laboratorytests were used. In the acute phase, SD Dengue Duo (NS1, IgG, IgM) rapid test showed IgG sensitivity 94.7% (18/19), specificity 92.9%(13/14), IgM sensitivity 85% (17/20), specificity 100% (13/13), NS1 sensitivity 50% (10/20), specificity 100% (13/13). In theconvalescent phase, SD Dengue Duo (NS1, IgG, IgM) rapid test showed antidengue IgG sensitivity 96.3% (26/27), specificity 66.7%(4/6), IgM sensitivity 95% (22/23), specificity 80% (8/10), NS1 sensitivity 42.9% (3/7), specificity 100% (26/26). To establish thediagnosis of dengue virus infection, not only NS1, but also antidengue IgM/IgG is needed. SD Dengue Duo containing dengue NS1 antigencombined with IgG/IgM test in one cassette is a rapid, practical and has a high validity diagnostic result.
KADAR KOLESTEROL HDL TERUKUR MENGGUNAKAN REAGEN CHOLESTEST N HDL DAN HDL-C PLUS GENERASI KETIGA Ichwan Meinardi; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.971

Abstract

The using of the open reagent system tools gives the possibility to choose the best quality of reagents including the reagent for HDLcholesterol concentration test. Hitachi 902 (Roche) as an open reagent system tool may used Cholestest N HDL (Daichi) as the firstHDL reagent and HDL-C plus 3rd generation reagent (Roche). The aim of this study was to know the correlation of HDL cholesterolconcentrations using Cholestest N HDL and HDL-C plus 3rd generation reagents measured by Hitachi 902. A cross sectional study wasdone from April to June 2008 at Ratulangi Medical Centre Laboratory, Makassar. The HDL cholesterol concentration was measured byHitachi 902 using Cholestest N HDL and HDL-C plus 3rd generation reagents. Sample was analyzed with SPSS 14 for Windows Programusing T test and Pearson Correlation. Among 80 samples we found the mean HDL concentration using Daichi reagent was 46.19 mg/dlranging from 34.99 mg/dl to 57.39 mg/dl and the mean using Roche 3rd reagent was 48.35 mg/dl ranging from 35.18 mg/dl to 61.52mg/dl, with p = 0.098 and Pearson Correlation was r = 0.967 with p = 0.000.There was no difference between HDL concentrationdetected by Cholestest N HDL and HDL-C plus 3rd generation reagents.
KADAR ALBUMIN SERUM PENDERITA STROK ISKEMIK DAN STROK HEMORAGIK Fasni Halil; Hj. Darmawaty ER; Ruland DN Pakasi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.956

Abstract

To differentiate the ischemic and hemorrhagic stroke with the most accurate method can be carried out by Computerized Tomography(CT) scan. However, because the restrictor on access and cost, not all patients could gain the advantage of CT scan. Examination ofalbumin is a quick and easy test. The aim of this study was to evaluate the serum albumin level in patient suffering ischemic andhemorrhagic stroke. A cross sectional study of 60 ischemic and hemorrhagic stroke patients was performed at the Neurologic Departmentof Dr. Wahidin Sudirohusodo Hospital Makassar, from March up to August 2008. Albumin – levels was measured using the colorimetricBCG method, using the Cobas Integra 400 Autoanalyser. The data were than analyzed with SPSS 11.5 software and t test. Among the60 samples of the ischemic and hemorrhagic stroke were found mean values of serum albumin level is 3.38 ± 0.120 and hemorrhagicstroke is 3.51 ± 0.0938 with p value is 0.495. There were no significantly different between the serum albumin level of the ischemicand hemorrhagic stroke patients.
KONFIRMASI FLU BABI A/H1N1 MENGGUNAKAN PCR A.A. Wiradewi Lestari; I.A. Putri Wirawati; Tjok Gde Oka
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.979

Abstract

Swine Influenza (2009 H1N1) is a new influenza virus causing illness in people. This new virus was first detected in the United Statespeople, April 2009. This virus probably spread the same way worldwide from person-to-person much as the regular spreading of commonseasonal influenza viruses. A 13 years old male entered the hospital with fever, cough and sore throat. Before he was hospitalized, hehad travelled to Batam for four (4) days. A PCR test from throat and nasal swabs were taken, and found positive for influenza A andswine H1 (as confirmed case for swine influenza A/H1N1). After taking oseltamivir for 5 days and the second PCR test negative, thepatient is released from the hospital.
Immature to Total Neutrophil (I/T) Ratio sebagai penunjang Diagnosis Sepsis Neonatorum Bastiana, Bastiana; Aryati, Aryati; Iriani, Yulia
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.967

Abstract

Early diagnosis of neonatal sepsis plays an important role in the management of patients. Blood culture, currently used as thegold standard, has several limitations such as time consuming and low positive rate. For this reason, a rapid and accurate diagnosticmethod is required. Manual differential count is a practical, inexpensive method and can support the diagnosis of bacterial infections.A shift to the left in differential white count with a raised immature neutrophil count has been documented in patients with bacterialinfections. This led to the use of I/T ratio as a indicator towards bacterial infections. The aim of this study is to obtain the diagnosticvalue of I/T ratio in diagnosing neonatal sepsis. The study was a prospective and cross-sectional. The subjects were enrolled consecutively,consisting of newborn babies (from birth to 30-days old) admitted to the Neonatal Intensive Care Unit (NICU) of the Dr. SoetomoHospital, Surabaya. Forty and three samples, consisting of 13 sepsis samples and 30 nonsepsis as controls samples were examined. I/Tratio are a ratio between immature neutrophils against total neutrophils in blood smear preparation. For the determination of the whitecell differential count, a total of 100 white cells (granulocytes) were counted. I/T ratio > 0.2 showed an abnormality that suggestaninfection process occur. Blood smear evaluations were done by three (3) independent observers. The result from three (3) observerswere as follows: sensitivity and specificity of I/T ratio in the diagnosis of neonatal sepsis were 69.2%, 92.3%, 61.5% and 50%, 50%,63.3%, respectively. Positive and negative predictive values were 37.50%, 44.44%, 42.10% and 78.94%, 93.75%, 79.16%, respectively.According to Cochran test there was no difference found between the 3 observers (p = 0.086). However, using Kappa test no agreementbetween I/T ratio and sepsis (p = 0.051) differences were found. from this study so far, the value in the diagnosis of neonatal sepsis theI/T ratio showed a low diagnostic.
POLA KETAHANAN (RESISTEN) DAN KEPEKAAN (SENSITIVITAS) KUMAN TERHADAP ANTIMIKROBA Y F Tallulembang; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.958

Abstract

In the developing countries including Indonesia, there still have problems with infectious diseases. Generally the hospitalized patientsin the Surgery section through have antimicrobial therapy. The treatment is intended to prevent nosocomial infection that usually foundin overnight treatment patients at the department of surgery. This study is carried out to know the pattern of micro-organism’s resistanceand sensitivity against various antimicrobial at department of surgery, Dr. Wahidin Sudirohusodo Hospital Makassar, January–June2008. A descriptive study of retrospectively collective data was carried out on 160 specimens by sensitivity test at the department ofsurgery, Dr. Wahidin Sudirohusodo Makassar Hospital, started from January up to June 2008. The five common bacterial found in thisstudy where Staphylococcus epidermidis, Klebsiella pneumonia, Enterobacter agglomerans, Staphylococcus saprophytic and Escherichiacoli. The sensitive antimicrobial drugs found where Vancomicin, Sulbactam/Cepoperazone, Cefepim, Gentamicin, Ceftazidim, Novobiocin,followed by the resistance antimicrobial were Methicillin, Tetracycline, Cefuroxim, Erythromycin, and Doxyciclin. The most bacterial typefound was Klebsiella pneumoniae, the most sensitive antimicrobial was Vancomicin and the most resistances were Methicillin.
RAGAM BERBAGAI PERBENIHAN BAKTERI TERKAIT KERENTANANNYA TERHADAP ANEKA JENIS ANTIBIOTIKA Carolina M Viany S; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.960

Abstract

Infection is the major public health problem in Indonesia which could increase its morbidity and mortality. The antibiotics treatmentwhich were given irrationally leads to bacteria susceptibility and worsen the problem. One of the efforts to manage the bacteriasusceptibility the physician has to know the bacterial pattern and its characteristic to resist various antibiotics. The information maycontribute as a reference to give antibiotic therapy in a rational manner. To know the bacterial susceptibility pattern against variousantibiotics a study was carried out using specimens derived from several hospitals which referred them to a private laboratory inSurabaya. The specimens consisted of blood, urine and sputum were referred during September 2007 up to July 2008. The identificationof the bacteria and it‘s susceptibility pattern were carried out by a conventional method and an auto analyzer (Vitek 2 Compact). Theantibiotic susceptibility test was carried out by conventional method using Kirby Bauer modifiied diffusion technique and Vitec 2 Compactusing MIC reference. The results showed that the most common bacteria found from blood was Escherichia coli which was still sensitiveto amoxicillin-clavulanic acid, amikacin, and cefepim. And from the urine was Escherichia coli which were still sensitive to meropenemfollowed by amikacin and gentamycin. Whereas from the sputum was found Streptococcus α haemolyticus which was still sensitive toamoxicillin-clavulanic acid, piperacillin tazobactam, linezolid. The result of the antibiotic susceptibility test is mostly dominated bythe Betalactam group, such as amoxicillin-clavulanic acid and carbapenem group like meropenem. Besides of that, in this study wasalso found multiple drug resistance organisms (MDRO), such as Escherichia coli ESBL, Enterobacter liquefaciens ESBL, Enterobacteragglomerans ESBL, Klebsiella ozaenae ESBL, and Klebsiella pneumoniae ESBL. The susceptibility pattern of the bacteria derived fromblood and sputum is dominated by gram positive cocci. Whereas from urine is dominated by Gram negative rods.
DIAGNOSIS MOLEKUL DAN APLIKASI DALAM PENGOBATAN HEPATITIS B & C Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.974

Abstract

In the whole world, up to now hepatitis B and C virus are still the main causes of chronic hepatitis, cirrhosis until hepatocellularcarcinoma. Most diagnosis is based on a serological examination such as the determination of antigen and antibody, for exampleHbsAg, HbeAg and anti HCV. Recently, for hepatitis C examination, HCV core antigen is used for the detection of HCV infection duringthe window period, chronic C hepatitis and for treatment monitoring. At present, serological assays are not sufficient to confirm thediagnosis of hepatitis B and C due to mutations of false negative HbsAg or HbeAg results. Occult hepatitis B can occur with a negativeresult of HbsAg, which cause difficulties in confirming the diagnosis and treatment as well. The success of treatment can be influencedof both hepatitis B and hepatitis C that have genotypes. By using molecular examination, such as determination of HBV-DNA and HCVRNA,it is expected that the problem of serological determination can be overcome. Molecular examination is not only useful for just thediagnosis confirmation, such as for active phase and replicative determination. This sequence is also very useful as a data base prior tothe treatment of hepatitis B and hepatitis C as well as for their following success result.
KINETIKA FAKTOR VON WILLEBRAND DEMAM BERDARAH DENGUE ORANG DEWASA Riat El Khair; Usi Sukorini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.965

Abstract

Dengue haemorrhagic fever (DHF) is still a problem in Indonesia. It is a syndrome that in most severe form may threaten the patient’slife, primarily through increased vascular permeability due to endothelial dysfunction leading to shock. Von Willebrand factor (vWf) is ablood glycoprotein involved in haemostasis and present in the blood plasma. The vWf is reported as one of dysfunction endothelial marker.However, there is limited information about the kinetics and contribution of vWf in the pathogenesis of DHF in adult patients. In thisstudy, a serial level of vWf was measured as kinetic of plasma vWf. It is expected that, the evidence based medicine will give contributionin the management of DHF patients. Also, in the future a study will be conducted especially about the prediction of shock to know thekinetic of plasma von Willebrand factor in adult dengue haemorrhagic fever patients. A cross-sectional repeated–measurement study wasconducted from October 2007 up to January 2008 in the Department of Clinical Pathology at the Sardjito General Hospital Yogyakarta.Subjects who met the eligible criteria were selected i.e. adult patients hospitalized in the Department of Internal Medicine diagnosed asDHF based on WHO criteria and antibody anti-dengue detection. Serial measurement of plasma vWf was determined on days five (5),seven (7) and 15 using enzyme linked fluorescent assay (ELFA) principle. The resulting data was shown graphically and the differencein levels of vWf among the three groups of time was analyzed by Friedman’s test. The study results showed an increase of vWf on dayfive /5 (218.48 %), followed by 187.08 % on day seven (7). Interestingly, there was a sharp increase of vWf on day 15 (233.80 %). Inaddition, there were statistically significant different levels of vWf among those three groups (p = 0.00) in adult dengue haemorrhagicfever patients with the von Willebrand kinetic factor showing a fluctuation pattern. There is an increased level of vWf on the fifth (5)day but a decrease on the seventh (7) day. However, there is a sharp increase in the convalescence phase.
DETEKSI MOLEKUL MUTASI GEN rpoB MYCOBACTERIUM TUBERCULO SIS PADA DAHAK DENGAN POL YME RASE CHAIN REACTION DAN SINGLE STRAND CONFORMATION POLYMORPHISM P B Notopuro; J Nugraha; H Notopuro
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.973

Abstract

Tuberculosis is a chronic infectious disease which is found in the developing as well as the developed country. This disease is oneof the community health problems which become the priority programs in the national as well as international health. In the lasttwo decades, they can be found in the emergency tuberculosis problems that is related with the Multi Drug Resistance (MDR) Strain.The detection of rifampicin resistance in M. tuberculosis infection can help clinical laboratory to find the MDR strain. Related to thisproblem the proportional culture method is still the gold standard for rifampicin resistance detection for M. tuberculosis infection. Butthis method needs 4−6 weeks to obtain the result, while its sensitivity is not very high. The development of the molecular detection forM. tuberculosis rifampicin resistance in a direct clinical specimen such as sputum, cerebrospinalfLuid, etc. will give an improvement inthe diagnosis, because it has an accurate, fast, sensitive and a specific result. Isolates from twenty six of M. tuberculosis derived fromthe sputum of tuberculosis patients that have failed the tuberculosis treatment, were examined with the proportional culture method.In this study PCR-SSCP were used for the molecular detection of rifampicin resistancy using direct sputum samples. The proportionalculture method was used as a gold standard for the rifampicin resistance detection. A set of primers was directed to conserve the regionof rpoB gene of M. tubercuLosis. This RNA polymerase gene was encondes?, which is bound on rifampicin. A 157-bp fragment wasamplified by PCR and analyzed by SSCP technique. The sensitivity of PCR-SSCP is 80% (high), its specificity is 95.2% (very high), thepositive predictive value is 80% and the negative predictive value is 95.2%. Statistically there were no significant difference between theresult of PCR-SSCP and the proportional culture method. Based on the study result, the molecular detection technique for rifampicinresistance on M. tuberculosis infection can be used as the screening device /means for Multi Drug Resistance Tuberculosis (MDR-TB),while the clinician waits the culure result.

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