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Contact Name
Dr. dr. Puspa Wardhani, SpPK
Contact Email
admin@indonesianjournalofclinicalpathology.org
Phone
+6285733220600
Journal Mail Official
majalah.jicp@yahoo.com
Editorial Address
Laboratorium Patologi Klinik RSUD Dr. Soetomo Jl. Mayjend. Prof. Dr. Moestopo 6-8 Surabaya
Location
Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Articles 10 Documents
Search results for , issue "Vol 17, No 1 (2010)" : 10 Documents clear
RESISTENSI TERHADAP METHICILLIN (METHICILLIN RESISTANT) STAPHYLOCOCCUS AUREUS DI INSTALASI RAWAT INAP Wildana .; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1047

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. MRSA infection typically aggravatesthe patient condition. MRSA infection increases morbidity and mortality. The study was aimed to find out the MRSA occurrence inDr. Wahidin Sudirohusodo Hospital Makassar patients during July 2008–June 2009. A retrospective study was performed using datafrom the medical records including the results of culture and antimicrobial susceptibility test in Dr. Wahidin Sudirohusodo HospitalMakassar. Among 1082 results of the culture test, 5.2% were identified as Staphylococcus aureus, consist of 51.8% MSSA (MethicillinSensitive Staphylococcus aureus) and 48.2% MRSA. Most of the MRSA patients were treated in orthopaedic surgery (30%), internal(22%), and paediatric (19%) wards. Based on the clinical conditions, most of the patients were in post surgery care (44.4%), pneumonia(18.5%), and diabetic foot (7.5%). All of the MRSA isolates were multiresistant (resistant to three or more antimicrobials) but 96%remain sensitive to vancomycin. It was concluded that most of MRSA patients were staying in the orthopaedic surgery ward. Based onthis clinical condition, most of the patients were in the post surgery care. All of the MRSA isolates were multiresistant, but most of themremain sensitive to vancomycin.
NILAI BATAS ANTIGEN NS1 DENGUE KUANTITATIF SEBAGAI PREDIKTOR KEPARAHAN JANGKITAN/TULARAN (INFEKSI) VIRUS DENGUE ANAK Betty A Tambunan; Aryati .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1044

Abstract

The diagnosis of viral dengue infection is very important for the management of dengue patients. In acute phase infection circulatingNS1 antigen can be detected in the sera of patients with dengue viral infection. This study is evaluating the NS1 antigen level in denguepatients using antigen captured ELISA Platelia TM Dengue NS1 Ag (Bio-Rad Laboratories). In this 30 examined dengue patients consistingof 3(10%) undifferentiated fever, 10(33.33%) dengue fever, 12(40%) DHF grade I, 2(6.66%) DHF grade II, 2(6.66%) DHF gradeIII, 1(3.33%) DHF grade IV. The result revealed that NS1 antigen was positive in 12 among 30 patients (40%) which were diagnosedas Dengue Viral infection based on 1997 WHO criteria. The sensitivity of NS1 antigen in these patients as confirmed with IgM andIgG antidengue serology test was 52.2%. The highest positivism of NS1 antigen was on the third day of fever. The results analyzed bySpearman correlation test revealed that there was no significant correlation between NS1 antigen level and the severity of dengue viralinfection. The cut-off value of quantitative NS1 antigen could not be determined because they were no significant correlation shown forNS1 antigen as the predictor for the severity of dengue viral infection. The conclusion of the study so far shown that the quantitativeNS1 antigen level could not be used as the predictor for the severity of dengue viral infection. The cut-off value of quantitative NS1antigen could not be determined because there were no significant correlation shown for NS1 antigen as the predictor for the severityof dengue viral infection.
UJI KESAHIHAN (VALIDITAS) PEMERIKSAAN D-DIMER CARA MENYARING KEKEBALAN (METODE IMUNOFILTRASI) DAN CARA MENGUKUR IMUNOTURBIDIMETRI David Rustandi; Delita Prihatni; Tiene Rostini; Nina Tristina
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1049

Abstract

D-Dimer parameter is the most usefull laboratory assay to detect the present of activated coagulation. The D-Dimer fragment directlyindicate the fibrinolitic proccess whereas the increasing mark of D-Dimer is the most hemostatic parameter that was used to detectthe early stage of Disseminated Intravascular Coagulation (DIC) and has a correlation with the patient prognosis. D-Dimer assay byimmunoturbidimetric method was used to detect antigen-antibody reaction automatically and it can detect D-Dimer concentration lessthan 0.5 μg/mL. The immunoturbidimetric method has a good correlation with the ELISA method, but the proccess is complicated, ishigh costed, and need the competence of practical human resource. D-Dimer assay with immunofiltration method has the same principleas immunoturbidimetric method, but it's work more simpler, low cost and does not need the competence of practical human resource.The aim of this study is to compare the D-Dimer assay concentration between immunofltration and immunoturbidimetric method. Therewere 30 plasma samples assayed with these two methods (immunoturbidimetric and immunofiltration), and then compared betweenthem. The samples were collected between November until December 2008 at Rumah Sakit dr. Hasan Sadikin Bandung. The validity ofD-Dimer using immunofiltration method showed sensitivity 74%, specificity 95%, predictive value of negative test (PV-) 22%, predictivevalue of positive test (PV+) 95% with likelihood ratio 5.2. The conclusion of this study so far that the immunofiltration method has agood validity for diagnosing. D-Dimer work rapidly with low cost laboratory assay than the immunoturbidimetric method.
AKTIVITAS FOSFOLIPASE-A2 SEKRETORIS PLASMA TROMBOSITOPENIA DEMAM BERDARAH DENGUE Endang Retnowati K; Wiyanda Hidayati S; Liana .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1042

Abstract

Infected macrophages by dengue virus will produce phospholipase-A2 (PLA2) enzyme, that can promote arachidonic acidmetabolism that produce inflammatory mediators, causing endhothelial damage and severe plasma leakage. Capillary endothelialdamage can cause platelet adhesion and aggregation, so that many platelets will be consumed. The role of sPLA2 (secretoryphospholipase-A2), which is a part of PLA2 in dengue and thrombocytopenia up to now has not been widely studied. The objective ofthe study is to analyze the association between the activity of plasma secretory phospholipase-A2 and the degree of thrombocytopeniain DHF adult patients. the study is carried out by a cross sectional, observational analytical study on 45 hospitalized adult patientssuffering dengue hemorrhagic fever in the Tropical Infection Ward, Department of Internal Medicine, Dr. Soetomo Hospital Surabaya,which has been conducted from February–December 2009. The diagnosis of Dengue Haemorrhagic Fever (DHF) was based on the1997 World Health Organization (WHO) criteria, that minimally had one positive serology marker of dengue. Venous blood wastaken from the patient for examining the activity of secretory phospholipase-A2 by correlated enzyme assay method, and plateletcount using automated hematology analyzer. The results of the secretory phospholipase-A2 activity and degree of thrombocytopeniawere analyzed by Pearson correlation test to determine the correlation between the two variables. In this study so far was foundthat the secretory phospholipase-A2 activity in DHF patients was 36.9–195.6 unit/mL (mean 97.49 unit/mL, SD 30.06 unit/mL).The mean of secretory phospholipase-A2 activity was increased according to the degree of thrombocytopenia severity. The mean ofsecretory phospholipase-A2 activities were 91.65 unit/mL, 98.94 unit/mL, and 110.47 unit/mL. The degree of thrombocytopeniawas divided into mild, moderate, and severe. Most of the patients showed mild thrombocytopenia. The sPLA2 activity in this studywas increased in DHF patients with second day of fever, and then decreased at the third and forth day of fever, and increased inDBD patients suffering fifth day of fever. The statistical analyzes show a non significant correlation between secretory phospholipaseA2 activity and degree of thrombocytopenia (p = 0.579). This result may be caused by several factors which influencing thethrombocytopenia in DHF, such as bone marrow suppression, dengue viral serotype, influence of cytosolic phospholipase-A2 (cPLA2)activity, and other proinflammatory cytokines which in this study could not be controlled. Statistical analyzes show a significantcorrelation between sPLA2 activity and the day of fever (p = 0.04). Further studies should have to be carried out in order to knowthe pattern of sPLA2 activity in DHF grade I, II, III, and IV, and to know the influence of other proinflammatory cytokines and viralserotypes in sPLA2 activities.
PROFIL VIRUS DENGUE DI SURABAYA TAHUN 2008–2009 aryati .; Puspa Wardhani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1046

Abstract

Four serotypes of dengue viruses (DENV) 1–4 are mosquito-borne human pathogens that cause widespread epidemics withconsiderable morbidity and mortality. The aim of this study was to evaluate the dengue serotypes profile, which were circulating inSurabaya. This research has been carried out consisting of 360 samples from patients with dengue virus infections, according the WorldHealth Organization (WHO) criteria. These sera were collected from patients Dr. Soetomo Hospital and private laboratory in Surabayafrom 2008–2009. From 360 samples, 68 samples (18.9%) were undifferentiated fever, 53 samples (14.7%) were dengue fever, 239samples (66.4%) were dengue hemorrhagic fever. From 58 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) samples, 25samples (43%) were positive, consisting of 52% DEN-2, 20% DEN-1, 16% DEN-3 and 12% DEN-4. These results showed that fourserotypes are circulating in Indonesia, dominated by DEN-2, followed by DEN-1, DEN-3 and DEN-4.
AIR KEMIH (URIN) BEREOSINOFIL DENGAN DUGAAN RADANG SELA GINJAL MENDADAK/NEFRITIS INTERSTISIAL AKUT (NIA) Felly G Sahureka; Fitriani Mangarengi; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1041

Abstract

The diagnosis of AIN is performed by the evaluation of clinical signs and symptoms, laboratory tests, radio imaging and biopsyas a gold standard. In most cases, biopsy wasn't performed because it is invasive for the patients, while the diagnosis is just based onthe clinical sign and symptom, and the immunosuppressive therapy is carried out only after the biopsy. Eosinophyluria found in theAIN patients is the parameter that can be measured non invasively, so that urine eosinophyl test was suggested for the diagnosis/earlydetection of AIN. That background cause us to analyze the urine eosinophyl count in suspected AIN patients. A cross sectional studywas conducted from June to August 2008 on 50 suspect AIN patients and 50 of non AIN at the Laboratory of Clinical Pathology,dr.Wahidin Sudirohusodo Hospital Makassar. Urine eosinophyl test performed by Hansel method, samples were analyzed with SPSSfor Windows version 12.0 using T test and Chi-square test. From 50 suspect AIN patients, they consist of 50% men and 50% womenwith the age distribution between 4 and 72 years old. T test analysis showed that the urine eosinophyl count was higher in suspect AIN(2.820 ± 1.955) compare with the non AIN (0.620 ± 0.923), p < 0.001. The Chi-square test showed that there was a significantrelation between eosinophyluria of the suspect AIN patients. That means there is a significantly relation between eosinphyiluria withthe suspect AIN group, where was found the higher urine eosinophyl compare to those non AIN patients. From this study so far, it canbe suggested that urine eosinophyl test can be used for the diagnosis/early detection of AIN.
KORELASI ANTARA NEURON-SPECIFIC ENOLASE SERUM DAN GLASGOW COMA SCALE DI PASIEN CEDERA KEPALA Usi Sukorini; Isti Setijorini Wulandari; Budi Mulyono; Handoyo Pramusinto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1043

Abstract

The outcome after head injury is mostly determined by Glasgow Coma Scale (GCS) and the degree of brain damage which reveals.CT scan is also important to assess its severity. However relatively it is not in a less costly manner and sometimes patients mobilisationare needed. Brain damage due to traumatic head injury refers to homeostasis unbalance, and it is the important causes of releasingbiochemical analyte from neuron via injured blood brain barrier to circulation. Neuron-specific enolase as a glycolytic enzyme in neuroncytoplasm might increase. Hopefully, measurement of NSE levels can provide information about the extent of the disease. The objectiveof the study is to test the correlation between the Neuron Specific Enolase (NSE) serum as a one of biochemical marker of brain injuryand the GCS. For this purpose, a cross sectional, analytical observasional study was carried out at the Emergency Departement andDepartement of Clinical Laboratory, Sardjito General Hospital, Yogyakarta, Indonesia. Fifty-one patients selected by an eligible criteriawere included in the study, which consist of severe, moderate and mild head injury. Blood samples were collected and serum NSE wasmeasured by immunoanalyzer using Electro Cheluminescence ImmunoAssay (ECLIA). Chi square test was used to test the differenceproportion of the group: NSE ≥ 21.7 ng/mL and NSE < 21.7 ng/mL according to measured variables, and Spearman correlation testwas used to correlate serum NSE and GCS, and other variables. In the study fifty-one patients with head injury were included, 74.5%of patients were males and 68.6% is in the age of 15–45 years old. The patients were further divided into two groups on the basis ofserum NSE ≥ 21.7 ng/mL and < 21.7 ng/mL; the former group was dominated by severe head injury patients (54.1%). In addition, aproportion of non survivors (66.6%) in group NSE ≥ 21.7 ng/mL was higher compared to those in NSE < 21.7 ng/mL group. Moreover,a large number of mild head injury (95.45%) and survivors (83.33%) had lower serum NSE (< 21.7 ng/mL). In the study, was found anegative correlation between serum NSE and GCS (r = -0.552; p = 0.00). Also, serum NSE were inversely correlated with blood kaliumand hemoglobin (r = -0.162; p = 0.027 dan r = -0.376; p = 0.009), in contrast with leucocytes count (r = 0.485; p = 0.001). Theconclusion so far there was a negative correlation between serum NSE and GCS. It is suggested that neuron-specific enolase can be veryuseful as a biochemical marker in assesssing the severity of head injury. Therefore, it is nessessary to carry out the prognostic study toknow to what extent it can predicting the outcomes.
PENGANGKAAN (KUANTIFIKASI) PERIKSAAN PULASAN GRAM DI BERBAGAI JENIS BAHAN PEMERIKSAAN Adhi Kristianto Sugianli; Ida Parwati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1048

Abstract

In a clinical microbiology laboratory the Gram staining is used to classify bacteria on the basis of their forms, sizes, cellularmorphologies, and Gram reactions. Additionally it is a critical test for rapid presumptive diagnosis of infectious agents and serves toassess the quality of clinical specimens. Several methods of Gram staining quantification are already applied: Canadian Coalition forQuality in Laboratory Medicine (CCQLM), Clinical Microbiology Proficiency Testing (CMPT), and World Health Organization (WHO).Each method consists of several criteria for quantification and its interpretation, such as neutrophil cell (polymorphonuclear cells),squamous epithelial cell, and number of microorganisms. Those methods aren't limited in sputum specimen, but also could be used forother specimen such as urine, vaginal discharge, and other body fluids. These methods are also could be used as screening for specimenbefore it is continued into further testing. Even though there is several limitation for each method, quantification method of Gramstaining could be provide better diagnostic value in microbiology laboratory as an early detection in the examination to get betterdiagnosis as well as treatment.
FLAMING CELLS DI MULTIPLE MYELOMA Nursin Abd. Kadir; Hj. Darmawaty E.R,; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1091

Abstract

Multiple myeloma is a type of cancer on plasma cells which are system of immune cells in bone marrow that produce antibodies. A47 years old man precented with an excruciatingly painfull bone lytic lesion acompanied with compressive fracture in his Thorakal XIIand first Lumbar vertebral body since a week ago. A complete blood count on admission showed anemia normocytic normocrom withhemoglobin content of 5.3 mg/dL. The blood smear revealed clumping of red blood cells to bound "Rouleaux formations". Serum proteinelectrophoresis showed specific evidence of a M-spike. Bence-Jones proteinuria was positive and serum kreatinin arised 2.44 mg/dL.The bone marrow aspiration contained 45% plasma cells, many of which exhibited the morphology of flaming cells with an eccentricnucleus and violaceous cytoplasm. Plasma cells varied in size and shape and included flaming cells and myeloma cells. The patient wasdiagnosed as having flaming cells in multiple myeloma stage IIIB.
PERAN POLIMORFISME GEN INTERFERON-g (IFNG) PADA FENOTIP HISTOLOGI NEFRITIS LUPUS Kusworini Handono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1045

Abstract

Lupus Nephritis (LN) is a serious complication of Systemic Lupus Erythematosus (SLE) with the development of end stage renaldisease in 10–70% patients within 5 years. The condition is classified into 6 different classes according WHO criteria. Several studiesshowed that there were significant clinical manifestation differences between class III, IV and class V LN. It has been suggested that theclass differences of LN was related to the cytokines balance and genetic factor. The objective of this study was to determine the role ofg-Interferron gene (IFNG) polymorphism in the class differences of LN. The study was conducted in 40 female SLE patients at the Dr.Saiful Anwar Hospital, Malang. Histologic phenotypes classification was based on World Health Organization (WHO) criteria (1995).Microsatelite polymorphism within the first intron of the IFNg gene on chromosome 12q24.1 was performed by DNA sequencing. Theallele difference between LN classes and healthy controle were analysed by Chi-square, the risk of LN in patients with certain IFNG allelewas calculated using Odds Ratio. The result showed that the frequency of IFNG 112 allele were higher in SLE patients compared withhealthy controls (succeptible allele) and the risk to have class V LN in patients with IFNG 112 was 6 times higher compared with patientswithout these allele. There is an association between IFNG polymorphism with the LN classes.

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