Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
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<![CDATA[OXIDIZED-LOW DENSITY LIPOPROTEIN DAN DERAJAT STENOSIS PENYAKIT JANTUNG KORONER (Oxidized-Low Density Lipoprotein and Stenosis Level in Coronary Artery Disease) ]]>
<![CDATA[Sutamti Sutamti]]>;
<![CDATA[Purwanto AP]]>;
<![CDATA[MI. Tjahjati]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1279
<![CDATA[Coronary artery disease is caused by atherosclerosis of the coronary arteries. Ox-LDL plays a role in atherosclerosis causing coronaryartery stenosis. There are differences in the research results on the relationship of ox-LDL levels and stenosis level of coronary arteryin patients with CAD. The aim of this study was to know the correlation between ox-LDL and stenosis level of coronary artery inCoronary Artery Disease (CAD). An observational study with cross sectional analytic approach was conducted on 37 patients with CADwho underwent coronary angiography in the Dr. Kariadi General Hospital and Telogorejo Hospital of Semarang, taken consecutivelyduring Febuary up to April 2014. The Ox-LDL levels were determined by sandwich ELISA and the stenosis levels were determined bycoronary angiography. The data were analyzed by non-parametric Spearman correlation test. Median of ox-LDL level in CAD patientswas 1666.8(846.15; 3324) pg/mL. The median of stenosis level was 80 (30;90)%. There was a significant correlation of ox-LDL leveland stenosis level (r=0.358; p=0.03) in CAD patients. Based on this study there was a weak positive correlation between ox-LDL leveland stenosis level in CAD patients.]]>
<![CDATA[PACKED RED CELL DENGAN DELTA Hb DAN JUMLAH ERITROSIT ANEMIA PENYAKIT KRONIS (Packed Red Cells with Delta Hb and Erythrocytes in Anemia of Chronic Disease) ]]>
<![CDATA[Novita Indayanie]]>;
<![CDATA[Banundari Rachmawati]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1270
<![CDATA[Anemia chronic disease is the second common cause after iron deficiency anemia with hemoglobin levels below the referencevalue. The pathogenesis of anemia should be determined for treatment. Hematinics and or erythropoietin are other treatments besidestransfusion. The transfusion is started when Hb≤7g/dL. The PRC transfusion of 4ml/kg could increase Hb level by 1 g/dL, or 1 unit andcould increase 3–5% of hematocrit. The objective of this study was to know the correlation of PRC unit with delta Hb and erythrocytesin anemia of chronic disease. The 60 samples examined were from patients of the Kariadi Hospital Semarang suffering from anemia ofchronic disease and who were transfused with PRC from January up to March 2014. The study subjects comprised 28 men (46.7%) and32 women (53.3%), with a mean age of 47 years. The number of PRC given was between one (1) to four (4) units. The mean delta Hbwas 3.48 and the mean delta erythrocytes was about 1.03 (0.1 to 2.3). There was a significant correlation between PRC units and deltaHb (r:0.856, p:0.000), as well as delta erythrocytes (r:0.716, p:0.000). Based on this study, it can be concluded that PRC units have avery strong correlation with delta Hb and as well as with delta erythrocytes in patients suffering from anemia of chronic disease]]>
<![CDATA[PROTEIN REKOMBINAN 38 KDA MYCOBACTERIUM TUBERCULOSIS DALAM INTERLEUKIN-2 DAN INTERLEUKIN-4 SERTA LIMFOSIT T CD3+ (The Mycobacterium Tuberculosis 38 kDa Recombinant Protein in Interleukin-2 and Interleukin-4 as well as CD3+ T Lymphocytes) ]]>
<![CDATA[Maimun Z Arthamin]]>;
<![CDATA[Nunuk S Muktiati]]>;
<![CDATA[Triwahju Astuti]]>;
<![CDATA[Tri Yudani M Raras]]>;
<![CDATA[Didit T Setyo Budi]]>;
<![CDATA[Francisca S. Tanoerahardjo]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1275
<![CDATA[Tuberculosis remains a serious global health problem despite the widespread use of the vaccine against tuberculosis (TB). Up tonow, the only available TB vaccine, Mycobacterium bovis BCG has a very wide efficacy range from 0 until 80 percent protection sothe development of a new vaccine is needed. The new protein as a candidate vaccine should be assessed for their immunogenicity. Thepurpose of this study was to examine whether Mycobacterium tuberculosis 38 kDa recombinant protein could stimulate a cellularimmune response especially CD3+T lymphocytes to express IL-2 and IL-4 in PBMC cultures. An experimental laboratory research oncultured PBMC of 3 groups consisting of TB patients, contacts of TB positive and healthy subjects, each group consisted of 8 subjects. AllPBMC cultures were induced by Mycobacterium tuberculosis 38 kDa recombinant protein, Purified Protein Derivative (PPD) and withoutantigen as a control. Expression of IL-2 and IL-4 CD3+ T lymphocytes was measured with flowcytometry. In healthy volunteers and TBcontacts there was a significant difference in the expression of IL-2 and IL-4 CD3+ T lymphocytes compared with no any treatment. Thehighest IL-2 expression was in healthy subjects [8.13 (0.622)] while the highest expression of IL-4 was in TB patients [6.436 (4.586)].Mycobacterium tuberculosis 38 kDa recombinant protein could induce the expression of IL-2 and IL-4 of CD3+ T lymphocytes in healthysubjects, TB contacts and TB patients and there were a significance differences in the expression of all groups.]]>
<![CDATA[HYBRIDIZATION-BASED NUCLEIC ACID AMPLIFICATION TEST TERHADAP CARTRIDGE-BASED NUCLEIC ACID AMPLIFICATION TEST TERKAIT MULTIDRUG-RESISTANT TUBERCULOSIS (Hybridization-Based Nucleic Acid Amplification Test towards Catridge-Based Nucleic Acid Amplification Test in Multidrug-Resistant Tuberculosis) ]]>
<![CDATA[Ivana Agnes Sulianto]]>;
<![CDATA[Ida Parwati]]>;
<![CDATA[Nina Tristina]]>;
<![CDATA[Agnes Rengga I]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1274
<![CDATA[Indonesia has high burden of multidrug-resistant tuberculosis (MDR-TB). Cartridge-based nucleic acid amplification test (CB-NAAT),which is recommended as a diagnostic method of MDR-TB by World Health Organization, is faster in achieving the result. This methoddetermines MDR-TB only from the rifampisin resistance, by detecting mutations that occur on the 81 bp hot-spot region of the rpoBgene. The isoniazid resistance is not included in the determination of MDR-TB by this method. Hybridization-based NAAT (HB-NAAT)detects MDR-TB not only from the rifampisin resistance (codon 526 and 531 rpoB gene), but also from the isoniazid resistance (codon315 katG gene). The aim of this study was to know the validity of the HB-NAAT in detecting MDR-TB using sputum with CB-NAATas the gold standard in a diagnostic study. All of 51 sputums were collected during June 2013 from patients suspected pulmonaryMDR-TB at Dr. Hasan Sadikin General Hospital. The result of CB-NAAT were 16 MDR-TB, 12 TB non MDR, and 23 non TB. HB-NAATexamination results were 3 MDR-TB, 25 TB non MDR (3 RMR, 6 IMR, 16 susceptible) and 23 non TB. The sensitivity of HB-NAAT was18.75% and specificity 100%. Low sensitivity values may due to the high mutation variations in the samples. So it could not be detectedonly by codons 526 and 531 for rifampisin resistance. For the detection of isoniazid resistance, HB-NAAT have optimal primer at lowconcentrations and it also need more than katG genes to detect isoniazid resistance. Based on this study, it can be conclued, that HBNAAThas low sensitivity but high specificity in the detecting MDR-TB.]]>
<![CDATA[MUTU LAYANAN MENURUT PELANGGAN LABORATORIUM KLINIK (Service Quality Regarding to The Clinical Laboratory Customer) ]]>
<![CDATA[Mohammad Rizki]]>;
<![CDATA[Osman Sianipar]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1287
<![CDATA[Customer satisfaction survey for outpatient laboratory customer is routinely carried out in Clinical Laboratory InstallationRSUP Dr. Sardjito using modified SERVQUAL questionnaire. One advantage of using SERVQUAL is service provider will be able to monitorits service quality dynamics using standardized tool. Changes in one’s service quality can be measured using perceived quality changesbased on gap scorebetween different periods. All these years, RSUP Dr. Sardjito has not evaluated gap score changes in all survey period.This study aims to know service quality measured by perceived service quality on Customer Satisfaction Survey Period of Semester II2013 and Semester I 2014 by comparison. This is an observational non-experimental study using data from Customer SatisfactionSurvey in Clinical Laboratory Installation RSUP Dr. Sardjito Semester II 2013 and Semester I 2014. Data was analysed using SERVQUALmethod and presented descriptively as text and table. There were 231 and 229 responders respectively in Customer Satisfaction SurveySemester II 2013 and Semester I 2014. There were decreases in all but empathy service dimension. Decrease of expectation was foundin all SERVQUAL dimensions. The decrease of expectation exceeded perception decrease resulting in a rise of gap score from Semester II2013 to Semester I 2014 hence indicating an improvement of perceived service quality. There was an improvement of perceived servicequality according to external customer of Clinical Laboratory Installation RSUP Dr. Sardjito in Semester I 2014 compared to SemesterII 2013.]]>
<![CDATA[PROTEIN 24 HIV DAN LIMFOSIT T-CD4+ DI INFEKSI HIV TAHAP I (HIV p24 Protein and CD4+ T-lymphocyte in Stage I HIV infection) ]]>
<![CDATA[I Made Sila Darmana]]>;
<![CDATA[Endang Retnowati]]>;
<![CDATA[Erwin Astha Triyono]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1280
<![CDATA[Measuring HIV p24 protein is a test which is more practical than determination of CD4+ T-lymphocyte counts and viral load, asit does not require a very sophisticated instrument and requires a lower cost. Independent predictive value of p24 to the decline ofCD4+ T-lymphocytes, clinical progression and survival in HIV-infected patients have been reported. In this study, HIV-infected patientswere found to have HIV p24 protein levels inversely proportional to CD4+ T-lymphocyte counts by using Spearman test (R2=0.225;p=0.0331). Studies on the correlation between HIV p24 protein levels and CD4+ T-lymphocyte counts in stage I HIV infection have notyet been reported. The aim of this study was to prove the correlation between HIV p24 protein levels and CD4+ T-lymphocytes in stageI HIV infection. Research issue was whether a correlation between HIV p24 protein levels and CD4+ T-lymphocyte counts in stage I HIVinfection existed ? The hypothesis was that a correlation between HIV p24 protein levels and CD4+ T-lymphocyte counts in stage I HIVinfection existed. The study design was cross sectional observational. Subjects consisted of 30 stage I HIV-infected patients treated at theInfectious Disease Intermediate Care Unit, Dr. Soetomo Hospital and VCT Clinic of the Dr. Ramelan Naval Hospital, Surabaya from Mayto July 2014. Stage I HIV infection is an asymptomatic HIV infection or with persistent generalized lymphadenopathy and the patientis able to perform normal activities. Levels of p24 were measured by ELISA method and CD4+ T-lymphocyte counts using flowcytometry(BD FACSCaliburTM). The results were statistically analyzed using Pearson’s correlation test. HIV p24 protein levels in stage I of HIVinfection ranged from 1.8 to 10.8 pg/mL, mean of 5.14 pg/mL and a standard deviation of 2.08 pg/mL. CD4+ T-lymphocyte countsdecreased with a range of 49-559 cells /uL for absolute values and 4.42–26.02% for percentage values Correlations between blood p24levels and CD4+ T-lymphocyte counts either absolute (r=–0.392, p=0.032) or percentage (r=–0.363, p=0.049) were found. In stageI HIV-infected patients, a negative correlation was found between p24 levels and CD4+ T-lymphocyte counts, in both CD4+T-lymphocytecounts as absolute and as well as percentage values. This negative correlation showed that the p24 HIV levels were inversely proportionalto the CD4+ T-lymphocyte counts. HIV p24 protein levels have a possibility to be used predicting CD4+ T-lymphocyte counts.]]>
<![CDATA[RERATA VOLUME TROMBOSIT DAN AGGREGASI TROMBOSIT DI DIABETES MELITUS TIPE 2 (Mean Platelet Volume and Platelet Aggregation in Diabetes Mellitus Type 2) ]]>
<![CDATA[Malayana Rahmita Nasution]]>;
<![CDATA[Adi Koesoema Aman]]>;
<![CDATA[Dharma Lindarto]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1283
<![CDATA[Diabetes mellitus patients often have hypercoagulable blood, as evidenced by the increased coagulation, impaired fibrinolysis,endothelial dysfunction and platelet hyperactivity. Hyperactive platelet is the major determinant of pro thrombotic state in DM. Byassessing the MPV and platelet aggregation, which is a marker of platelet activity, in patients with type 2 DM, it is expected to help theprediction of acute events. This research is aimed to know the differences of MPV and the aggregation of platelet between poor glycemiccontrol as well as good the control group in type 2 DM patients. This study was conducted in cross sectional method using 22 people withgood glycemic control and 28 people with poor one (glycemic control) from June to August 2013. Fasting blood samples were analyzedfor CBC, HbA1c, TG and platelet aggregation. MPV and platelet aggregation value were compared between groups using independentt-test. Based on this study, there is no significant difference in MPV and platelet aggregation between groups (p=0.598, p=0.464 (1 μM),p=0.868 (2 μM), p=0.984 (5 μM), p=0.401 (10 μM)). Mean Platelet Volume (MPV) correlate significantly with platelet aggregationat 1 μM and 5μM ADP concentration in good glycemic control group (r=0.591; p=0.004 at 1 μM ADP and r=0.521; p=0.013 at 5 μMADP). Mean platelet volume correlate significantly with the platelet aggregation at 2 μM ADP and the concentration in poor glycemiccontrol group (r=0.405; p=0.033). There are no significant differences in MPV and platelet aggregation between groups, but there is asignificant correlation between them (MPV and platelet aggregation) in the good glycemic control of the type 2 DM group.]]>
<![CDATA[INDEKS ATEROGENIK PLASMA DI INFARK MIOKARD AKUT DAN PENYAKIT DIABETES MELITUS (Atherogenic Index of Plasma in Acute Myocardial Infarction and Diabetes Mellitus) ]]>
<![CDATA[Zulfikar Indra]]>;
<![CDATA[Suci Aprianti]]>;
<![CDATA[Darmawaty E.R.]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1271
<![CDATA[Diabetes Mellitus (DM) is associated with an increased cardiovascular risk. Atherogenic Index of Plasma (AIP) is a strong predictorof myocardial infarction. The aim of this study was to know the difference of AIP in acute myocardial infarction (AMI) with and withoutDM. This study was conducted by cross sectional method using data from the medical records of AMI patients at the Dr. WahidinSudirohusodo Hospital, Makassar during January 2010 up to May 2013. The AIP values were calculated by the [log (TG: HDL-C)]formula. All data were classified into two groups. The data were then analyzed by unpaired T test. In this study, AMI was mostly foundin the 50–59 years group, 33.63%. The AIP in AMI with DM was higher than without DM (0.69±0.26 vs 0.57±0.26, p=0.001). TheAIP in AMI with and without DM was higher in the female than the male group (0.63±0.24 vs 0.62±0.28. P=0.58). The AtherogenicIndex of Plasma in AMI with DM was higher than without DM. Based on this study, it can be concluded, that AIP can be used as thepredictor of cardiovascular risk in diabetes patients.]]>
<![CDATA[ANGKA BANDING ALBUMIN KREATININ AIR KEMIH DAN HbA1C SERTA ESTIMASI LAJU FILTRASI GLOMERULUS PADA PASIEN DIABETES MELITUS TIPE 2 (Urinary Albumin to Creatinine Ratio With HbA1c and Estimated Glomerulo Filtration Rate in Type 2 Diabetes Mellitus Patients) ]]>
<![CDATA[Amiroh Kurniati]]>;
<![CDATA[Tahono Tahono]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1276
<![CDATA[Diabetes Mellitus (DM) type 2 is a metabolic disease that prevalence increasing. A chronic hyperglycemia with poor glycemiccontrol can stimulate oxidative stress, which will continue to occurrence of complications in the kidneys characterized by the presenceof microalbuminuria can be measured by the ratio of urinary albumin creatinine ratio (UACR) and the change in estimated glomerularfiltration rate (eGFR). The aims of this study was to know the correlation between the UACR with HbA1c value and eGFR in patients withtype 2 DM by finding them out. This study used cross sectional research design. Subjects were patients with type 2 DM who attend controlin Endocrinology Subdivision of Internal Medicine Departement and perform blood and urine tests in Clinical Pathology Laboratory inDr. Moewardi Hospital Surakarta in August 2013. To determine the pattern of the data distribution, the researchers used KolmogorovSmirnov test, and to analyse the result used Spearman (r) correlation with p<0.05 and confidence interval 95%. Statistical analysisusing Spearman correlation test (r), significant when p<0.05 with 95% confidence intervals. From 68 samples examined the meanage is 60.9 year old, with equal participants for male and female (34 subjects each). Most subjects were in poor glycemic control group(72.1%) and in the range of microalbuminuria (44.1%). There was a significant correlation between UACR with HbA1c and eGFR intype 2 DM patient (r=0.412, p=0.000; and r= -0.270, p=0.02, respectively). Based on this study it can be concluded that increasedUACR were associated with worsened glycemic control and were characterized by higher levels of HbA1c and its eGFR value would belower. Further analysis requires further research with a larger sample size and more attention to the factors that may affect the relatedexamination.]]>
<![CDATA[NILAI RUJUKAN SOLUBLE TRANSFERRIN RECEPTOR (sTfR) {(Soluble Transferrin Receptor Refence Value (sTfR)} ]]>
<![CDATA[Anggraini Iriani]]>;
<![CDATA[Endah Purnamasari]]>;
<![CDATA[Riadi Wirawan]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i3.1268
<![CDATA[Iron in plasma is carried by transferrin delivered to cells through the interaction with a specific membrane receptor, namelytransferrin receptor. The soluble transferrin receptor (sTfR) is a transferrin receptor monomer which lost its first 100 amino acids, andcirculates in the form of transferrin and its receptor complex. Erythroblasts and reticulocytes are the main source of serum TfR Theconcentration of sTfR in serum is useful to diagnose iron deficiency, especially for patient with chronic disease. A new parameter sTfRis reported to be a surrogate marker of bone marrow iron store. The sTfR concentration can describe the functional iron status whileferritin reflects the iron storage status. The aim of this study was to know a reference interval of sTfR in normal adults by provision.Subjects were 157 healthy adults from clinical medical check up who had met the inclusion criteria and were willing to participate asresearch subjects. Soluble Transferrin Receptor (sTfR) examination was performed using reagents from Roche. The statistical calculationswere performed by SPSS 22. The results showed that there was no significant difference between sTfR levels in men and women as wellas in the age group ≤40 years and >40 years. The STfR reference value in this study was calculated based on 95% CI (X±2SD), is0.197–0.598 mg/dL. It can be concluded that the sTfR reference value is 0.197–0.598 mg/dL.]]>
