Garuda - Garba Rujukan Digital

Kandungan Isovlavon dan Vitamin E pada Formula Kombinasi Tepung Tempe dan Bekatul Untuk Memperbaiki Profil Lipid Darah Sufiati Bintanah; Siti Fatimah Muis; Purwanto AP
Jurnal Gizi Vol 7, No 2 (2018): Jurnal Gizi UNIMUS
Publisher : Universitas Muhammadiyah Semarang

The prevalence of cardiovascular disease continues to increase as a result of dietary changes that have an impact on abnormal lipid profiles and oxidative stress. Efforts to improve lipid profiles byconsuming food contain antioxidant compounds that have the potential to improve blood lipid profiles. The aim of the study was to obtain a description of the content as an antioxidant in the formula of the combination of soybean tempeh flour with rice bran.Research method: This type of experimental research is to find out the most optimum antioxidant content of the formula. Making formulas, (1) yellow soybean tempeh flour + white bran flour, (2 black soybean tempeh flour + white bran flour (3) yellow soybean flour tempeh + red bran flour,(4) black soybean flour tempeh + red bran flour, with composition of 1: 1 and weighs 112 grams each, non-calorie sweetener 3 grams and food flavorings 3 grams, dissolved in cold water as muchas 250 cc, analysis of nutrients using the AOAC method, 2005 . Data analysis: Data obtained are averaged and displayed in table form, then compared between treatments. Results: The combination of YST + RBF with a composition of 1: 1 has a higher isovlavone content while the highest content of vitamin E is found in the combination of YST + WBF. Conclusion: There is sufficient isovlavone and tokoverol content from a combination of tempeh flour and bran to improve blood lipid profile.Keywords: Isoflavones, vitamin E, formula of tempeh and bran flour, blood lipid profile

PHYLLANTHUS NIRURI L TERHADAP IMUNITAS SELULER TIKUS Ima Arum L; Purwanto AP; Henna Rya
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 1 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i1.358

Typhoid fever is a problem in the developing country. Phyllanthus niruri L (meniran) is a traditional plant that can be used as an immunomodulator on cellular or humeral immunity. By giving Phyllanthus niruri L extract to patient who suffers typhoid fever can increase cellular immunity responses in intracellular bacteria infection (S.typhimurium).This research was an experimental study using the post test-only control group design to proof of the effects of Phyllanthus niruri L on cellular immunity balb/c infected by salmonella typhimurium. The samples were taken from thirty male Balb/c mice which were divided into five groups including P1 (infected by S.typhimurium and PnL extract 125 g), P2 (infected by S.typhimurium and PnL extract 250 g), P3 (infected by S.typhimurium and PnL extract 500 g), K1 (infected by S.typhimurium) and K2 (healthy mice). In this study was applied parametric test of one way ANOVA and post hoc test Bonfferoni. It was known that phyllanthus niruri L increased the number of activated lymphocytes and phagocyte ability of macrophage (p=0.003 and p=0.001, respectively). By Bonfferoni post hoc test resulted that no significantly difference between the treated groups (increased of dose). There were no significant difference between the number of leukocyte, the difference count of lymphocyte and the nitrite oxide produced among the treatment groups and controls. Phyllanthus niruri L dose 250 μg increased the activated lymphocyte and index phagocytes. To find out the effects of Phyllanthus niruri L compare to antibiotic and Phyllanthus niruri L combination, continuing/advancing studies are needed

VOLUME PLASMA DAN FAKTOR VIII DALAM KRIOPRESIPITAT Dian Widyaningrum; Purwanto AP; Julia Setyati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 1 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i1.396

Blood product such as cryoprecipitate required a quality control. This includes development, implementation and the standard operating procedures use of each step of the process in the production of cryoprecipitated substance to ensure that the produced product contains a minimum of 80 international units (IU) of factor VIII. Cryoprecipitation is prepared from fresh frozen plasma that thawed and centrifuge by immediate spinning the excess plasma which then removed and leaving approximately 40ml which deposit 10 mL cryoprecipitate. One unit of cryoprecipitate contain 70–80 IU/unit factor VIII, ≥100 mg/unit von Willebrand factor, fibrinogen 5–10 mg/dL. The levels of factor VIII and von Willebrand factor (VWF) lowered in individuals with blood group O compared to individuals groups with non-O blood. This research is aimed to investigate whether plasma volume are correlated with the levels of factor VIII in cryoprecipitation. In this study purposive sampling is done in which 25 bags of cryoprecipitate materials (was storage for 11 months) from all types of blood group which were taken from storage, thawed, weighed and the plasma volume measured. Factor VIII was measured by coagulometric method. The researcher used Spearman correlation test to analyze the product, with significance degree p<0.05 and confidence interval 95%. In this study it is found plasma volume which was not related to the factor VIII level in cryoprecipitattion substance (p=0.585). Mean plasma volume of the cryoprecipitated matter was 56 mL, mean factor VIII was 83.3UI. Highest factor VIII level was 160.6 UI of cryoprecipitated blood group AB and lowest factor VIII level was 21.3 UI of cryoprecipitated blood group A.

KADAR ASAM URAT SERUM DAN KOMPONEN SINDROM METABOLIK MI Diah P; Banundari Rachmawati; Purwanto AP
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.469

The incidence of metabolic syndrome (MetS) is significantly increasing. The elevated serum uric acid (SUA) level is correlated with the metabolic risk of cardiovascular. The relationship between SUA levels and components of MetS has been inconsistent. The aim of this study was to know the correlation between SUA level and component of MetS by analyzng. This study was a cross-sectional design conducted on healthy people at the Medical Check Up (MCU) Clinic, Dr. Moewardi Hospital, Surakarta from June 2012 until February 2013. MetS was defined according to the 2005 International Diabetes Federation (IDF) criteria. The statistical analysis was done by Student’s T test, Pearson or Spearman correlation coefficients. The researchers considered p<0.05 as statistically significant with a 95% confidence interval. A total of 80 healthy subjects, (range 19–57 years) were enrolled into this study. MetS was diagnosed in 40 persons where men (62.5%) were more than women and the non-MetS in women (62.5%) were more than men. In this study, a positive correlation was found between SUA and systolic blood pressure, diastolic blood pressure and waist circumference (Pearson r=0.354, 0.495, 0.399, respectively, p<0.05) in women, but not in men. The SUA was positively correlated with systolic blood pressure, diastolic blood pressure and waist circumference, particularly in women. A long term study is needed to investigate the consequence of increasing the SUA level in metabolic disorders and hormonal influences as well.

OXIDIZED-LOW DENSITY LIPOPROTEIN DAN DERAJAT STENOSIS PENYAKIT JANTUNG KORONER (Oxidized-Low Density Lipoprotein and Stenosis Level in Coronary Artery Disease) Sutamti Sutamti; Purwanto AP; MI. Tjahjati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1279

Coronary artery disease is caused by atherosclerosis of the coronary arteries. Ox-LDL plays a role in atherosclerosis causing coronaryartery stenosis. There are differences in the research results on the relationship of ox-LDL levels and stenosis level of coronary arteryin patients with CAD. The aim of this study was to know the correlation between ox-LDL and stenosis level of coronary artery inCoronary Artery Disease (CAD). An observational study with cross sectional analytic approach was conducted on 37 patients with CADwho underwent coronary angiography in the Dr. Kariadi General Hospital and Telogorejo Hospital of Semarang, taken consecutivelyduring Febuary up to April 2014. The Ox-LDL levels were determined by sandwich ELISA and the stenosis levels were determined bycoronary angiography. The data were analyzed by non-parametric Spearman correlation test. Median of ox-LDL level in CAD patientswas 1666.8(846.15; 3324) pg/mL. The median of stenosis level was 80 (30;90)%. There was a significant correlation of ox-LDL leveland stenosis level (r=0.358; p=0.03) in CAD patients. Based on this study there was a weak positive correlation between ox-LDL leveland stenosis level in CAD patients.

RESIDU LEUKOSIT DALAM THROMBOCYTE CONCENTRATE Nurmalia PS; Purwanto AP; Julia Julia
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 1 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i1.394

Transfusion reactions in the thrombocyte concentrate (TC) manual is often associated with the storage time due to the release of cytokines by leukocytes during storage. The results showed the majority of transfusion reactions include fever/febrile non-haemolytic transfusion reaction (FNHTR). The quality control of blood components is necessary for the determination a component of blood in the bag already qualified for or not for transfusion. The aims of this study is to determine the residual leukocytes in the TC manual of Blood Donor Unit (UDD) at PMI Semarang with the shelf life of 3−5 days in compliance with the requirements of TC quality criteria of the European Union Council (EU).A Descriptive study is conducted at the Blood Donor Unit (UDD) PMI Semarang. The TC sample consist of 30 bags and manuals from the transfusion bag, which has been stored between 3−5 days. The examination of the residual leukocytes from the TC bag is carried out by manual microscopic method as well as the counting. The results obtained from the O blood group are 13 bags (43%) of TC, the B blood group seven (7) bags (33%), A blood group two bags (7%), and AB five (5) bags (17%). The range of the residual values of white blood cells in the TC manual is from 0 up to 0.0086×109/unit. According to the EU Council of leukocytes in the TC control value is <0.2×109/unit. The results of the residual leukocytes in the TC manual with the shelf life of 3−5 days are in accordance with the quality standards. This results indicates that the residual leukocytes in the TC manual UDD PMI Semarang in accordance with the EU quality standards.

MONOCYTE LYMPHOCYTE RATIO IN DENGUE HEMORRHAGIC FEVER Dwi Retnoningrum; Purwanto AP
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1130

Dengue Hemorrhagic Fever (DHF) adalah penyakit yang disebabkan oleh virus dengue, virus yang dapat ditularkan melalui gigitannyamuk. Dengue hemorrhagic fever merupakan salah satu penyebab kesakitan dan kematian terbanyak di dunia termasuk di Asia.Patogenesis infeksi DHF diduga melibatkan monosit dan limfosit akibat dari respons imun terhadap infeksi. Monocyte Lymphocyte Ratio(MLR) sebelumnya digunakan dalam menggambarkan respons imun di infeksi malaria, tuberkulosis dan HIV. Penelitian ini bertujuanuntuk mengetahui apakah terdapat perbedaan MLR di infeksi DHF derajat ringan dan berat. Metode penelitian ini observasional analitikdengan desain potong lintang di pasien DHF di RS Dr. Kariadi Semarang masa waktu Januari-Desember 2013. Nilai MLR didapat dariperhitungan jumlah monosit dibagi jumlah limfosit dari hitung jenis lekosit. Derajat DHF ditentukan sesuai dengan patokan WHO, yaituderajat I-II masuk dalam derajat ringan, derajat III-IV adalah derajat berat. Analisis statistik dengan Student t test. Kelompok I terdiridari 40 pasien DHF derajat ringan dan kelompok II terdiri dari 40 pasien DHF derajat berat. Subjek terdiri dari 41 laki-laki (51,2%)dan 39 perempuan (48,8%). Rentang nilai MLR di DHF derajat ringan ditemukan dari 0,03–0,33 (median 0,11) sedangkan di DHFderajat berat dari 0,03–0,59 (median 0,16). Analisis statistik menunjukkan tidak terdapat perbedaan nilai MLR antara kelompok I(derajat ringan) dan kelompok II (derajat berat) (p=0,08). Tidak didapatkan perbedaan nilai MLR di infeksi DHF derajat ringan danberat.

UJI DIAGNOSTIK PLASMODIUM MALARIA MENGGUNAKAN METODE IMUNOKROMATOGRAFI DIPERBANDINGKAN DENGAN PEMERIKSAAN MIKROSKOPIS Ima Arum L; Purwanto AP; Arfi S; Tetrawindu H; M Octora; Mulyanto Mulyanto; Surayah K; Amanukarti Amanukarti
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 3 (2006)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i3.871

Microscopic examination is still a gold standard for malaria diagnostic tests. Immunochromatographic (IC) technique can be usedas an alternative examination. The aim is to compare the identification value of immunochromatographic method diagnostic test tomicroscopic examination of malaria in laboratory examination. Cross sectional diagnostic study approach was applied to those withsymptoms of: (1) fever (temperature > 38°C) or intermittent fever lasting for two days or more (2) cephalgia/myalgia, 604 sampleswere taken consecutively in January to July 2005 from primary health centres of Sukaraja and Keruak, as well as Clinic Nugraha atLombok Timur district , province of Nusa Tenggara Barat . From these samples, there were 37 samples with P.vivax, 45 samples with P.falciparum, 5 samples with mixed infection and 517 samples without Plasmodium sp. From IC examination, results were reserved for 36samples with P. vivax, 69 samples with P. falciparum, 5 samples with mixed infection and 503 samples without Plasmodium sp. Diagnostictest data analysis showed that the immunochromatographic test revealed 100% sensitivity, 96.7% specificity, 83.2% positive predictivevalue and 100% negative predictive value. It can be concluded that malaria IC test is reliable to be used as a routine malaria test.

Title

Found 8 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 8 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 8 Documents
Search