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Achmad Arifiyanto
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Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 242 Documents
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Toxicity Test of Turmeric Extract on Wader Fish (Rasbora sp.) Embryogenesis Activity: Uji Toksisitas Ekstrak Kunyit terhadap Aktivitas Embriogenesis Ikan Wader (Rasbora sp.) Septriani, Nur Indah; Adha, Muhammad Nurul Fathoni; Sa'diyah, Maftuhatus; Septiani, Chelsy Ammara; Amaliah, Meinawa; Bagus, Tsaqifa Suhayra Emery; Natania, Soen Eliora Valerie; Prasetyaningrum, Enjang Sekaryati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Turmeric (Curcuma longa) is a medicinal plant containing active compounds, mainly curcumin, which may affect fish embryonic development, an essential stage for species survival. A previous study reported that exposure to chlorpyrifos affected the embryonic development of the wader pari fish Rasbora lateristriata, confirming its suitability as a local model for embryotoxicity testing. Based on this evidence, the present study evaluated the potential toxic effects of turmeric extract during the early stages of embryo development. Toxicity was assessed using turmeric extract concentrations of 50 ppm and 100 ppm in wader fish (Rasbora sp.). The methods included turmeric solution preparation, broodstock selection and spawning, egg collection, treatment grouping, and embryo observation at 0, 24, 48, and 72 hours. Observed parameters were pigmentation, mortality rate, and embryonic heart rate. The results showed that turmeric extract at both 50 ppm and 100 ppm exhibited toxic effects on Rasbora sp. embryos, especially on pigmentation. Higher curcumin concentrations were associated with lower pigmentation levels. Embryo mortality reached 11.1% at 50 ppm and 5.56% at 100 ppm, caused by embryotoxic effects that disrupted embryonic development and induced egg coagulation. Heart rate was slightly lower than the control group, indicating curcumin’s influence on cardiac activity and cellular signaling pathways.
Utilization of Citrus reticulata, Blanco Tangerine Peel Remains as an Ovicide to Prevent Hatching of Aedes aegypti Mosquito Eggs: Pemanfaatan Limbah Kulit Jeruk Keprok (Citrus reticulata Blanco) Sebagai Ovisida dalam Menghambat Penetasan Telur Nyamuk Aedes aegypti Emantis Rosa; Hafidzah Nabila; Priyambodo; Endah Setyaningrum
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 13 No. 1 (2026): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v13i1.457

Abstract

Dengue fever is a global health problem that requires natural-based vector control strategies as an alternative to synthetic insecticides. This study aimed to identify secondary metabolites and evaluate the efficacy of Citrus reticulata (Blanco) peel extract as an ovicide in preventing the hatching of Aedes aegypti mosquito eggs. Extraction was performed using the maceration method with 70% ethanol for five days, followed by phytochemical analysis and ovicidal activity testing at concentrations of 0.3%, 0.5%, 1%, 5%, and 10% with four replicates using 25 eggs per treatment. Data were analyzed using the Shapiro-Wilk test and Tukey's extended test. Phytochemical testing revealed secondary metabolites of flavonoids, phenols, tannins, saponins, alkaloids, and steroids. Tukey's test showed the ovicidal efficacy of Citrus reticulata (Blanco) peel extract in preventing the hatching of Aedes aegypti eggs. The highest concentration was at 10%, with 23.75±0.957 unhatched eggs (98%), and
Galangin, Galanal A, and α-Farnesene as Anticancer Candidates for Breast Cancer: A Molecular Docking Study: Galangin, Galanal A, dan α-Farnesene sebagai Kandidat Antikanker Kanker Payudara: Kajian Molecular Docking Gumilang Mutiara Hilia; Indah Rakhmawati Afrida; Aulya Nanda Prafitasari
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 13 No. 1 (2026): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v13i1.459

Abstract

Breast cancer is one of the leading causes of cancer-related deaths among women worldwide. The development of anticancer agents derived from natural compounds continues to be explored due to their high bioactive potential and relatively low toxicity. This study aims to evaluate the anticancer potential of Galangin, Galanal A, and α-Farnesene derived from Alpinia purpurata against breast cancer using an in silico approach. Molecular docking was conducted to analyze binding affinity and residue interactions between the test compounds and the target protein. The docking results showed that Galangin had the lowest binding energy at -235.43 cal/mol, followed by Galanal A (-206.01 cal/mol) and α-Farnesene (-196.48 cal/mol), indicating stable interactions. All three compounds interacted with key residues such as PHE71, LYS75, and GLU68 within the active site of the target protein. Galangin exhibited the highest binding affinity and the greatest number of interacting residues, making it the most promising candidate for inhibiting breast cancer. These findings suggest that Galangin, Galanal A, and α-Farnesene have potential as anticancer agents against breast cancer and warrant further investigation through experimental studies.
Bird Nest Inventories and Characteristics in Fragmented Area: Case Study in Universitas Bangka Belitung Campus: Inventarisasi dan Karakteristik Sarang Burung di Area Terfragmentasi: Studi Kasus di Kampus Universitas Bangka Belitung Nur Annis Hidayati; Ramadani; Novia Lesti; Rani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 13 No. 1 (2026): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v13i1.465

Abstract

Birds play a variety of ecological roles as bioindicators of ecosystem health, where their reproductive success and nesting behavior are influenced by the diversity of habitat vegetation. Although some areas implement green development concepts, fragmentation due to urbanization in these areas could alter microhabitat structure and affect the presence and reproductive success of local birds. This study aims to inventory and identify the characteristics of bird nests on the Bangka Belitung University (UBB) campus as baseline data to guide management in mitigating the impacts of development on fauna sustainability. This study was in March 2020. Data collection was conducted using the transect method, with a total transect length of 9 km. The presence of bird nests was determined by visual observation. From the observations, 20 bird nests were found, 16 of which were active nests. Based on their characteristics, the nest found can be grouped into four nest types. Five types of plants were identified as used as nesting sites, including kedebik, saga, ketapang kencana, cempedak, and seru. Kedebik (Melastoma malabathricum L.) and Ketapang kencana (Terminalia mantaly H.Perrier) are the most common nest tree in our study area. The nests found are cup-shaped with a basal attachment type, typical nests of birds belong to Order Passeriformes. The nests are composed of natural materials, such as grass, ferns, dry leaves, twigs, and feathers. It is hoped that the reported findings can be used as consideration in the future development of the UBB campus
Phytochemical Analysis of the Roots of Solanum diphyllum L. as a Potential Exotic Weed in Purwosari Village: Studi Fitokimia Akar pada (Solanum diphyllum L). sebagai Gulma Eksotis yang berpotensi di Kalurahan Purwosari Fitria Alfiyah; Muzzazinah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 13 No. 1 (2026): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v13i1.466

Abstract

This study aimed to analyze the phytochemical constituents of the roots of Solanum diphyllum L., an exotic species that has naturalized in Purwosari Village. Root samples were extracted by maceration, followed by qualitative phytochemical screening using tube tests to detect alkaloids, flavonoids, and phenolic compounds. The results showed positive reactions for alkaloids and flavonoids, while phenolic compounds were not detected in the root extract. These findings indicate that the roots of S. diphyllum contain specific secondary metabolites with potential biological relevance. This study provides preliminary information that may support further phytochemical investigations and contribute to considerations for the utilization and ecological management of the exotic species S. diphyllum in tropical environments.
Contrasting Effects of Intensive Cultivation and Paddy Field Abandonment on the Invasion of Ludwigia grandiflora (Michx.) Greuter & Burdet in the Lowlands of Manokwari, Indonesia: Pengaruh Kontras Lahan Sawah Intensif dan Lahan Bekas Sawah terhadap Invasi Ludwigia grandiflora (Michx.) Greuter & Burdet di Dataran Rendah Manokwari, Indonesia Slamet Arif Susanto; Pramesty Wulan Ramadhanty; Trigarcia Maleachi Randa
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 13 No. 1 (2026): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v13i1.471

Abstract

The invasive plant Ludwigia grandiflora (Michx.) Greuter & Burdet poses a serious threat to tropical wetland ecosystems, particularly abandoned paddy fields. The invasive potential of this plant results from the interaction between its regenerative capacity, as reflected by population size, seed production, soil seed bank, and germination rate. This study aimed to analyze individual abundance, soil seed bank potential, and germination rates of L. grandiflora across two land-use types, namely intensively cultivated paddy fields and abandoned paddy fields that have been left uncultivated for five years, in Sidey District, Manokwari Regency, West Papua, Indonesia. Abundance was assessed using the quadrat method, while seed production and germination rates were evaluated through seed counts per capsule and the seedling emergence method at two soil depths. The results showed that the abundance of L. grandiflora in intensively cultivated fields ranged from 80 to 160 individuals m⁻², whereas in abandoned paddy fields it ranged from 50 to 90 individuals m⁻². Welch’s t-test revealed that the abundance of L. grandiflora differed significantly between the two land types studied (p < 0.05). Likewise, the number of seeds per capsule was significantly higher in intensively cultivated paddy fields (p < 0.05). In contrast, abandoned paddy fields exhibited a higher soil seed bank and higher germination rates (p < 0.05) than intensively cultivated fields. Soil samples from greater depths showed lower germination rates of L. grandiflora, as well as longer germination times, compared with surface soils. The abundance, seed production, soil seed bank, and germination rate can serve as early indicators of the invasive potential of L. grandiflora. These findings indicate that agricultural activities, particularly fertilization, play an important role in enhancing the invasive potential of L. grandiflora in lowland paddy field ecosystems
Salicylic Acid-Mediated Mitigation of Root Anatomical Degradation in Rice (Oryza sativa L.) Cultivars with Varying Salt Tolerance: Mitigasi Degradasi Anatomi Akar Berbasis Asam Salisilat pada Kultivar Padi (Oryza sativa L.) dengan Tingkat Toleransi Garam yang Berbeda Hana Widiawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 13 No. 1 (2026): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v13i1.473

Abstract

Salinity is an abiotic stressor that severely limits rice productivity worldwide by inducing osmotic stress and ionic toxicity. This study aimed to evaluate the ameliorative effects of foliar-applied salicylic acid (SA) on the root anatomical characteristics of three rice cultivars with varying sensitivity: pigmented rice ‘Sembada Hitam’ (pigmented), compared to white rice ‘Ciherang’ (moderately tolerant), and ‘IR-64’ (sensitive). The role of exogenous SA in mitigating salinity stress in rice is well-documented regarding shoot biomass, ion homeostasis and antioxidant defense mechanisms, its explicit impact on internal root anatomical plasticity remains poorly understood. The plants were grown in a soil-compost medium (3:1) and subjected to 100 mM NaCl stress starting at 30 days after planting (DAP). Salicylic acid (1 mM) was applied foliarly at 25, 50, 75, and 90 DAP. Root anatomical parameters, including root diameter (RD), cross-sectional area (CSA), cortical radius (CR), and metaxylem characteristics, were observed through transverse sections at the end of the vegetative phase. This study established a definitive link between foliar-applied SA and the preservation of critical root microstructures. The results indicated that salinity stress significantly reduced RD, CSA, CR, and stele across all cultivars. The sensitive cultivar ‘IR-64’ exhibited the most pronounced anatomical degradation, particularly in sclerenchyma thickness and metaxylem development. Conversely, ‘Ciherang’ showed higher structural resilience under saline conditions. The application of 1 mM SA successfully mitigated these negative impacts, particularly in ‘IR-64’ by significantly increasing root diameter, cortical radius, and the number and diameter of metaxylem vessels. This study concluded that exogenous SA application is an effective strategy to enhance the salt tolerance of rice by preserving critical root anatomical integrity.
Analysis of In Silico Interaction Campasterol of Alphitobius diaperinus with Interleukin 1β in Burn Conditions: Analisis Interaksi In silico Campasterol Kumbang Alphitobius diaperinus dengan Interleukin 1β pada Kondisi Luka Bakar Yossi Maryanti; Erfan Aritonang; Rahmawati Januar; Akbar Wicaksono; Yuli Susana; Detty Intan Sari; Irva Riandika; Putri Jelita Manulang; Fatmawati; Sri Wahyuni
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 13 No. 1 (2026): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v13i1.485

Abstract

Burns are a pathological condition that, if not managed properly, will cause wider damage. This study aims to analyze the potential of campasterol from the cage beetle Alphitobius diaperinus inhibitor IL-1β in burn conditions analyzed through in silico. The receptor used is Interleukin-1β. The test ligand used is campasterol. The comparator ligand used is silver sulfadiazine. The study variables analyzed were affinity energy, root mean square deviation (RMSD), amino acid bonds, inhibition constants, and binding site similiarity percentage (%BSS). The results showed that the RMSD value of IL-1β was 0.4626 Å. The energy value of campasterol affinity showed the most negative value compared to natural ligands and comparators, which was -6.4 kcal/mol. Campasterol amino acid bonds have six hydrophobic bonds, while silver sulfadiazine has only one bond. Based on the results of in silico analysis, it is known that campasterol has the potential to be an IL-1β inhibitor candidate in burn conditions.  
Histochemical Analysis of Secondary Metabolite Compounds in Zinnia elegans from Tambakaji, Ngaliyan, Semarang, Central Java: Uji Kandungan Senyawa Histokimia pada Tumbuhan Zinnia elegans di Tambakaji Ngaliyan Semarang Jawa Tengah Endah Hidayatul Fuadiah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 13 No. 1 (2026): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v13i1.486

Abstract

The presence of Zinnia elegans in the Tambakaji area has so far been viewed primarily from an aesthetic perspective, despite its biological potential through secondary metabolite content that requires further investigation. This study critically determines the localization of secondary metabolite compounds in various plant organs to map the pharmacological capacity of the species. The study was conducted using a qualitative descriptive approach with fresh preparation techniques and specific reagents. The observation results showed an organ-specific distribution of secondary metabolite; alkaloids, phenols, and tannins were strongly identified in root and stem tissues, while flavonoids and lipids were not detected in any organ. These findings indicate that the distribution of secondary metabolite in Zinnia elegans is not evenly spread across all organs. This study provides fundamental information for the utilization of Zinnia elegans as a source of natural bioactive raw materials and serves as an important reference for the development of its pharmacological potential.
Analysis of The Water Quality Index (WQI) of Residential Well in Bandar Lampung City: Analisis Indeks Kualitas Air (IKA) Air Sumur Penduduk di Kota Bandar Lampung Elisa Rindiyani Vatresia; Tugiyono; Agus Subagyo
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 13 No. 1 (2026): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v13i1.491

Abstract

Well water is the primary source of clean water for a portion of the population in Bandar Lampung City that has not been served by the municipal water supply (PDAM); therefore, its quality needs to be comprehensively evaluated to ensure safe daily use. This study aimed to analyze the water quality status using the Water Quality Index (WQI) based on the Regulation of the Ministry of Environment and Forestry of Indonesia (PerMenLHK) No. 27 of 2021, Appendix I. Water samples were collected from nine well water sampling stations across Bandar Lampung City. The analyzed parameters included turbidity, color, odor, pH, total dissolved solids (TDS), nitrate, nitrite, manganese, iron, hexavalent chromium, Escherichia coli, and total coliform. The results showed that most parameters met the water quality standards stipulated in the Regulation of the Ministry of Health of the Republic of Indonesia No. 2 of 2023, except for nitrate at Station 1, E. coli at Stations 7 and 8, and total coliform at Station 9. Based on the Pollution Index (PI) analysis, Stations 2, 3, 4, 5, and 6 met the quality standards, Stations 1 and 9 were classified as lightly polluted, while Stations 7 and 8 were categorized as moderately polluted. Based on the PI values, the WQI of well water in Bandar Lampung City was classified as moderate, with a WQI value of 56.6. This study provides an overview of the well water quality status and can serve as a basis for the management and control of clean water quality in urban areas.

Page 24 of 25 | Total Record : 242

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