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Pengembangan Produk Wedang Uwuh Bunga Turi (Sesbania grandiflora) Sebagai Sarana Branding Desa Wisata Kebonagung, Imogiri, Bantul Septriani, Nur Indah; Arsanti, Faulindra Dita; Sholihati, Febina; Ahmad, Datu Maulana
Jurnal Pengabdian, Riset, Kreativitas, Inovasi, dan Teknologi Tepat Guna Vol 1 No 2 (2023): November
Publisher : Direktorat Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/parikesit.v1i2.9600

Kebonagung tourist village offers a variety of tourist destinations ranging from agricultural education, traditions, to crafts. As a complement, the manager of the tourist village provides a welcoming drink for visiting tourists. However, there has not been a welcoming drink and typical souvenirs from the Kebonagung Village. The dedication aims to create product processing innovations from local resources. The KKN-PPM UGM from Imogiri unit took the initiative to develop the Vegetable Hummingbird Wedang Uwuh product. This dedication begins with a preliminary survey, then continues with the process of developing wedang uwuh products using the drying method. At the end of the dedication activity, socialization was carried out regarding the production process of Vegetable Hummingbird Wedang Uwuh and a survey has done through interviews to determine the level of success of the dedication activity. Making Vegetable Hummingbird Wedang Uwuh begins with washing the ingredients thoroughly, sun drying the ingredients, drying the ingredients using an oven at 50°C, then packing the dry ingredients in tea bags to make the product easier to consume. The product is also repackaged in a paper pouch by paying attention to the packaging design so that a product prototype is produced. Vegetable Hummingbird Wedang Uwuh product development program is quite successful. This is proven by the results of interviews regarding the level of participants' understanding of the techniques for processing and packaging designs for Vegetable Hummingbird Wedang Uwuh. The commitment of local SME actors also supports the goals of sustainable development or Sustainable Development Goals (SDGs).

Natural Dye as an Alternative to Hematoxylin-Eosin Staining on Histological Preparations Karlina, Ina; Pusparini, Nur Ainun Oktavia; Maharesi, Chesa Ekani; Saeed, Faisal; Retnoaji, Bambang; Saragih, Hendry; Septriani, Nur Indah; Rohmah, Zuliyati; Hadi, Susilo; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.7909

Hematoxylin-eosin is widely utilized in the field of animal microtechniques. However, the need to develop alternative dyes from natural sources such as plants has gained attention. Several studies have shown that many plants contain secondary metabolites with the potential to be developed as natural dyes. Lonchocarpus cyanescens and Syzygium cumini are promising candidates as alternative dyes for hematoxylin, while Lawsonia inermis and Hibiscus sabdariffa have shown potential as substitute dyes for eosin. These plants contain various secondary metabolites, including anthocyanins, flavonoids, chlorophyll, betalains, alkaloids, saponins, tannins, carbohydrates, proteins, phenolics, terpenoids, quinones, coumarins, xanthones, and resins. L. cyanescens exhibits a strong binding affinity to cells and tissues, particularly testicular tissue. Dyes derived from Syzygium cumini have been shown to provide a good staining result for rat liver cells. In contrast, dyes from Lawsonia inermis can stain cytoplasmic components and muscle fibers. Additionally, the dye from Hibiscus sabdariffa is capable of staining various biological components, including sperm, nerve cells, and blood cells. The dye preparation process involved extraction from different plant organs, such as leaves, flowers, and fruit. These findings suggest that secondary metabolites from these four plants hold significant potential for development as natural dyes to replace hematoxylin-eosin in histological applications.

International Bibliometric Analysis of Literature on Intrauterine Insemination Septriani, Nur Indah; Paramita, Pradnya; Firdiana, Vykra Aulia; Norfita, Tia Silva; Sayidinar, Aisha
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p079-087

Intrauterine insemination (IUI) is an artificial insemination method that employs specialized devices to introduce spermatozoa into the uterus. IUI is utilized to address challenges associated with poor reproductive outcomes and to optimize the productivity of livestock. Consequently, its application and utilization has gained global attention and is being continuously developed. Therefore, this study aimed to analyze the global literature on intrauterine insemination research over the preceding decade. Documents related to intrauterine insemination research were presented in the results of a bibliometric study indexed in the Scopus database for the period 2012–2022. On a global scale, the total number of identified documents amounted to 2,721, with an average annual production of 272 documents. Ten countries worldwide were identified as leading contributors to research publications on intrauterine insemination, including the United States with the most document production (n=643), followed by Turkey (n=175), India (n=173), China (n=161), United Kingdom (n=159), Iran (n=153), Netherlands (n=152), Canada (n=140), France (n=131), and Italy (n=129). This study of novelty comprehensive bibliometric analysis to map a decade of global research trends in intrauterine insemination across both human and animal applications. Unlike previous narrative reviews that focused on clinical or technical aspects, this research highlights global collaboration networks, publication dynamics, and emerging thematic hotspots within the IUI domain. Publications related to intrauterine insemination showed an upward trajectory from 2012 until 2021, followed by a decline in 2022. The findings from this analysis provide valuable guidance for future research in the field of intrauterine insemination.

Pengaruh Fucoidan Terhadap Struktur Hepar Ikan Zebra (Danio rerio, Hamilton 1822) yang Diberi Parasetamol Dosis Tinggi Septriani, Nur Indah; Simanungkalit, Eben Ezer; Mayani, Olvita; Fauziah, Indah Nur; Saputri, Desy Amelia; Sitepu, Anggi Rehulina; Ali, Raafi Nur; Adaninggar, Ascarti; Dewayani, Raden Roro Risang Ayu; Raharjeng, Anita Restu Puji
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.27185

AbstrakN-asetil-p-aminophenol atau parasetamol merupakan analgesik dan antipiretik yang mudah didapat tanpa resep. Penyalahgunaan dan kesalahan dosis konsumsi dapat menyebabkan kerusakan hepar. Fucoidan memiliki aktivitas hepatoprotektif yang dapat menstimulasi regenerasi hepatosit. Studi ini bertujuan untuk mengamati struktur histologis hepar ikan zebra (Danio rerio, Hamilto 1822) dewasa yang telah diberi perlakuan parasetamol dan fucoidan. Desain penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan ikan zebra dibagi menjadi empat kelompok perlakuan yaitu kontrol (K) selama 10 hari, parasetamol 5 mM (P) selama 3 hari dilanjutkan air RO selama 7 hari, parasetamol 5 mM selama 3 hari dilanjutkan fucoidan 100 µg/mL (P + F 100) selama 7 hari dan parasetamol 5 mM selama 3 hari dilanjutkan fucoidan 500 µg/mL (P + F 500) dengan ulangan setiap kelompok 6 ekor ikan. Studi berlangsung selama 10 hari, parameter yang diamati adalah histopatologis hepar, berat badan ikan, keaktifan berenang, dan nafsu makan. Pada semua kelompok perlakuan, hasil menunjukkan bahwa struktur histologis hepar ikan zebra mengalami kerusakan jaringan berupa hemoragi, dan kerusakan sel berupa vakuolisasi, piknosis dan nekrosis. Terdapat penurunan aktivitas berenang dan nafsu makan setelah perlakuan parasetamol. Kelompok yang diberi fucoidan mengalami pemulihan aktivitas renang dan nafsu makan. Terdapat perbedaan nyata (P <0,05) kerusakan hepar antara perlakuan kontrol, parasetamol dan pemberian fucoidan. Kelompok P + F 100 dan P + F 500 mengalami pemulihan hepatosit setelah paparan parasetamol. Kelompok P + F 500 memiliki perbaikan yang lebih baik dibandingkan kelompok P + F 100. Hasil pada penelitian ini adalah fucoidan dapat digunakan sebagai agen protektif hepar setelah paparan parasetamol dosis tinggi.AbstractN-acetyl-p-aminophenol or paracetamol is an analgesic and antipyretics which can be obtained easily without a prescription. Consumption misuse and wrong dosage intake can lead to liver damage. Fucoidan has hepatoprotective activity that can stimulate hepatocyte regeneration. The aim of this study was to observe the histological liver structure of adult zebrafish that had been treated with paracetamol and fucoidan. The research design used was a completely randomized design (CRD) with zebrafish divided into four treatment groups; control (K) for 10 days, paracetamol 5 mM (P) for 3 days followed by RO water for 7 days, Paracetamol 5 mM for 3 days followed by fucoidan 100 g/mL (P + F 100) for 7 days and Paracetamol 5 mM for 3 days followed by fucoidan 500 g/mL (P + F 500) with replicates for each group of 6 fish. The study lasted for 10 days, data on liver histopathology was evaluated, fish body weight, swimming activity, and appetite was also evaluated. In all treatment groups, the results showed that the histological structure of the zebrafish liver experienced tissue damage in the form of hemorrhage, and cell damage in the form of vacuolization, pyknosis and necrosis. There was a decrease in swimming activity and appetite after paracetamol treatment, the group given fucoidan experienced a recovery in swimming activity and appetite. There was a significant difference (P <0.05) in liver damage between the control, paracetamol and the fucoidan treated groups. The P + F 100 and P + F 500 exhibited hepatocyte recovery after exposure to paracetamol. The P + F 500 group had better improvement than the P + F100 group. The result of this research showed that fucoidan can be used as a protective liver agent after paracetamol high dosage exposure.

Natural Dye as an Alternative to Hematoxylin-Eosin Staining on Histological Preparations Karlina, Ina; Pusparini, Nur Ainun Oktavia; Maharesi, Chesa Ekani; Saeed, Faisal; Retnoaji, Bambang; Saragih, Hendry; Septriani, Nur Indah; Rohmah, Zuliyati; Hadi, Susilo; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.7909

Hematoxylin-eosin is widely utilized in the field of animal microtechniques. However, the need to develop alternative dyes from natural sources such as plants has gained attention. Several studies have shown that many plants contain secondary metabolites with the potential to be developed as natural dyes. Lonchocarpus cyanescens and Syzygium cumini are promising candidates as alternative dyes for hematoxylin, while Lawsonia inermis and Hibiscus sabdariffa have shown potential as substitute dyes for eosin. These plants contain various secondary metabolites, including anthocyanins, flavonoids, chlorophyll, betalains, alkaloids, saponins, tannins, carbohydrates, proteins, phenolics, terpenoids, quinones, coumarins, xanthones, and resins. L. cyanescens exhibits a strong binding affinity to cells and tissues, particularly testicular tissue. Dyes derived from Syzygium cumini have been shown to provide a good staining result for rat liver cells. In contrast, dyes from Lawsonia inermis can stain cytoplasmic components and muscle fibers. Additionally, the dye from Hibiscus sabdariffa is capable of staining various biological components, including sperm, nerve cells, and blood cells. The dye preparation process involved extraction from different plant organs, such as leaves, flowers, and fruit. These findings suggest that secondary metabolites from these four plants hold significant potential for development as natural dyes to replace hematoxylin-eosin in histological applications.

Review: Aplikasi Histopatologi Untuk Praktik Forensik: Review: Applications of Histopathology for Forensic Practice Faulana, Arum Fahmi; Empra, Desi Eka Putri; Rahmadian, Desti; Shalihah, Fianicha; Nuriliani, Ardaning; Retnoaji, Bambang; Saragih, Hendry Tri Sakti SG; Rohmah, Zuliyati; Septriani, Nur Indah
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.6582

Forensics plays an important role in investigating crimes because it can reveal everything related to a murder case, including the time and cause of the murder. Histopathology is the study of damage to body tissues and organs which can be used to help investigate crimes. The aim of this literature review is to study the role of histopathology applications for forensic practice. The method used is searching journals and books using Google Scholar, ScienceDirect, NCBI, and PubMed with the keywords histopathology, forensics, diagnosis, death, microscopy, disease, staining, and autopsy. The histology approach helps the pathologist make a clear determination on the cause of death. The aim of histopathology is to perfect the macroscopic diagnosis and act as an additional examination in forensic case management. One of the histopathology methods in the forensic field is the paraffin method with hematoxylin eosin staining and special stains, immunohistochemistry, and MITS. The parameters observed with this method are measurements of pulmonary fat embolism; determining the age of myocardial infarction; assessment of liver steatosis, fibrosis, and cirrhosis; determination of the age of thrombus or thromboembolism; and diagnosis of shock with various diseases. The prospect of forensic histopathology is to complement molecular diagnostic methods, so that the combination of molecular and histology methods can play a maximum role in the field of forensic pathology. The conclusion of this literature review is that histopathology can be used to confirm the diagnosis in forensic cases and has good future prospects if combined with other methods such as molecular.

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