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Achmad Arifiyanto
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Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 231 Documents
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The Potency of Organic Fertilizer in Reducing Leaf Curl Disease on Chili (Capsicum annuum L.): Potensi Pupuk Organik Dalam Mengurangi Penyakit Keriting Daun Pada Cabai (Capsicum annuum L.) Subiastuti, Aprilia Sufi; Windianingsih, Diah Fitri; Noviyani, Pipit; Kusnanda, Prima Sekti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 1 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v12i1.418

Abstract

Chili is an essential commodity in Indonesia, with demand continuing to rise; however, its production is often disrupted by leaf curl disease caused by viruses. One potential solution is using organic fertilizer enriched with beneficial microbes such as Plant Growth-Promoting Microorganisms (PGPM). Despite its potential, the role of PGPM in controlling viral diseases in chili plants has been rarely studied. This study aims to explore the effectiveness of organic fertilizer in reducing leaf curl infections by improving plant health. Two chili cultivars were used: 'Gama LBF' (a breeding product of PIAT UGM) and 'Ponirun' (a commercial variety known for its disease resistance). The chili plants were treated with various concentrations of organic fertilizer, and their effectiveness was evaluated based on disease incidence (the number of symptomatic plants) and disease severity (the level of visual symptoms), which were then converted into an index. Results showed that the 75% concentration yielded the lowest disease incidence in both cultivars—26.5% for 'Ponirun' and 42.85% for 'Gama LBF'. The lowest disease severity was also observed at the 75% concentration for 'Ponirun' and 50% for 'Gama LBF'. These findings highlight the importance of selecting the appropriate organic fertilizer concentration to suppress leaf curl disease in chili effectively.
Macrobenthic Community Structure as a Bioindicator of Environmental Quality at Pier 1, Kota Agung: Struktur Komunitas Makrobenthos sebagai Bioindikator Kualitas Lingkungan di Dermaga 1, Kota Agung Pawhestri, Suci Wulan; Vantika, Marsela Ayu; Andandaningrum, Della; Ardianti, Indah Marlina
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 1 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v12i1.421

Abstract

This study aims to assess the water quality of Pier 1 Kota Agung using macrobenthic community structures as biological indicators. Coastal areas such as Kota Agung Pier are increasingly exposed to anthropogenic pressures due to port operations, shipping activities, and surrounding land use. These pressures can subtly alter water quality and benthic community structure over time. A descriptive quantitative approach was applied across three observation stations representing the aquatic conditions surrounding the pier. Macrobenthos samples were collected using a Macrobenthic sample, which was carried out using a 10-inch core sampler and a 10mm mesh, for physical and chemical parameters. The identified macrobenthic organisms belonged to the classes Gastropoda, Oligochaeta, and Malacostraca, showing moderate diversity (H’ = 1.337–1.567), high evenness (E = 0.84–0.98), and low dominance (D = 0.24–0.37). The Family Biotic Index (FBI) values ranged from 3.81 to 3.86, indicating good water quality with low levels of organic pollution. Physical and chemical parameters such as temperature, water clarity, pH, and dissolved oxygen were within the standard quality thresholds. The study reveals that the environmental conditions at Pier 1 Kota Agung are relatively good, as indicated by balanced macrobenthic community structures and supportive physicochemical parameters.
Botanical, Pharmacognosy, and Phytochemical Studies of Sintrong Leaves (Crassocephalum crepidioides) Growing in Tanjung Setia Village, West Coast, Lampung: Kajian Botani, Farmakognosi, dan Fitokimia Daun Sintrong (Crassocephalum crepidioides) yang Tumbuh di Desa Tanjung Setia Pesisir Barat Lampung Anindita, Reza; Pratama, Gita Cahya; Putri, Intan Kurnia; Beandrade, Maya Uzia; Futihat, Chilyati Eky
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 1 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v12i1.423

Abstract

Crassochepalum crepidioides plant has antibacterial and anti-wound properties. This study aims to determine the botanical profile, microscopic powder, phytochemical screening, and Thin Layer Chromatography (TLC). This study aims to determine the morphology of leaves, stems, roots, microscopic powder, phytochemical screening, and Thin Layer Chromatography (TLC) of C. crepidioides leaves from Tanjung Setia Village West Coast Lampung, Indonesia. Methods include morphological examination, microscopic powder, phytochemical screening, and TLC. The results of the botanical show oval-shaped leaves, pointed tips, pinnate veins, serrated edges, smooth leaf surface, soft wood stems, and tap roots. Microscopic observation found fragments of stomata. Positive phytochemical screening of alkaloids, flavonoids, saponins, and tannins. TLC test spot with five spots (red) and an average Rf value of 0.21-0.97. Concludes that the thin layer chromatography test is thought to produce flavonoid spots at an Rf value of 0.72-0.77.
Aquatic Insect Diversity as Bioindicators of Water Quality in Organic Inorganic Rice Fields, Pringsewu, Lampung: Keanekaragaman Serangga Air sebagai Indikator Kualitas Air di Sawah Organik Anorganik di Pringsewu Lampung Pramudita, Rima; Dania Pratami, Gina; Meidaliyantisyah; Handayani, Kusuma
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v12i2.424

Abstract

The diversity of aquatic insects serves as an important indicator for assessing the quality of agricultural ecosystems, particularly in organic and conventional (non-organic) rice fields. Organic farming, which relies on natural inputs, tends to support greater biodiversity, whereas conventional farming may reduce it due to the use of chemical substances.   This   study   aimed   to   examine   the diversity, dominance, evenness, and environmental factors influencing the presence of aquatic insects in two types of agricultural systems in Tulung Agung Village, Lampung. The results showed that the organic   rice   fields   had   higher   aquatic   insect diversity, with a diversity index (H') of 2.3386, a dominance index (D) of 0.058, and an evenness index (E) of 0.9411. In contrast, the conventional rice fields showed a diversity index (H') of 0.9743, a dominance index (D) of 0.5188, and an evenness index (E) of 0.3312. Environmental factors had no significant effect on aquatic insects in the organic fields, but showed a strong correlation in the conventional fields, with a correlation value (r) of 0.905 and a significance value (p) of 0.035. These findings highlight that organic farming systems are better able to support a more stable aquatic ecosystem balance.
Effect of Juice Berry Fruit (Antidesma bunius (L.) Spreng.) on Total Cholesterol Levels in Mice (Mus musculus Linnaeus, 1758) with Hypercholesterolemia: Pemberian Jus Buah Buni (Antidesma bunius (L.) Spreng) terhadap Kadar Kolesterol Total pada Mencit Jantan (Mus musculus Linnaeus, 1758) Hiperkolesterolemia Isnaini, Nurul; Nurcahyani, Nuning; Fithria Mumtazah, Dzul; Sutyarso
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v12i2.427

Abstract

Hypercholesterolemia is influenced by excessive fat consumption. To lower blood cholesterol levels, both drug and non-drug therapy can be carried out. One plant that has the potential to lower cholesterol levels is the buni plant (Antidesma bunius (L.) Spreng.). This study aims to determine the effect of buni fruit juice and the most effective juice concentration in lowering total cholesterol levels in hypercholesterolemic male mice. This study used a completely randomized design consisting of 5 groups, each repeated 5 times. The experimental animals were given a High Fat Diet (MDTL) containing propylthiouracil (PTU) at a dose of 1.8 mg/200g BW suspended in 2% Pure Grain Alcohol (PGA) and 10 mL/KgBW of quail egg yolk. The treatments consisted of a negative control (standard feed); a positive control (MDTL and 0.52 mg simvastatin); P1 (MDTL and 1 ml of 20% buni fruit juice); P2 (MDTL and 1 ml of 25% berry juice); and P3 (MDTL and 1 ml of 30% berry juice). Treatments P1, P2, and P3 were administered MDTL for 14 days, followed by berry juice for another 14 days. The results were total blood cholesterol levels. Data were analyzed using a one-way ANOVA followed by Duncan's test at a confidence level of α = 5%. The results showed that administration of 30% berry juice reduced total cholesterol levels in male mice with hypercholesterolemia.
Daily Behavior of Javan Gibbon (Hylobates moloch) Ragunan Wildlife Park: Implications for Reintroduction and Welfare Assesment: Perilaku Harian Owa Jawa (Hylobates moloch) di Taman Margasatwa Ragunan: Implikasi terhadap Reintroduksi dan Kesejahteraan Satwa di Konservasi Ex-Situ Gustimaya, Nizarani; Pusparini, Fitria
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v12i2.433

Abstract

The Javan gibbon (Hylobates moloch) is a primate endemic to Java that has been classified as endangered by the IUCN since 2015. Conservation efforts are carried out through breeding and ex-situ conservation. This study aims to analyze the daily behavior patterns of Javan gibbons at Ragunan Wildlife Park and assess their relationship with animal management, including cage conditions, feed, and enrichment as a basis for recommendations to improve animal welfare. The study was conducted on two male and female individuals using the scan sampling method, focusing on feeding, resting, moving, and social activities. Data analysis was conducted using qualitative-descriptive methods. The results showed that the dominant activity was movement (≥20%), followed by resting (15%), feeding (≥6%), and socializing (≥3%). Activity increased in the afternoon between 2:50 p.m. and 3:00 p.m. This pattern differs from the behavior of gibbons in their natural habitat, which has the potential to reduce their natural abilities and hinder the success of their release. This study provides important empirical data on the behavioral adaptation of Javan gibbons in captivity, which has rarely been studied in the context of ex-situ conservation in Indonesia.
Diversity of Shrimp Species Based on Fishermen's Catches in the Coastal Area of Pematang Cengal Village, Langkat Regency, North Sumatra: Keanekaragaman Jenis Udang Berdasarkan Hasil Tangkapan Nelayan Kawasan Pesisir Desa Pematang Cengal, Langkat, Sumatera Utara Jayanthi, Sri; Astuti, Sri Pujianti; Nur, Siti Fadhilah; Nabila, Shifa; Pepayosa, Enca
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The This study aims to determine the diversity, morphology, and morphometric studies of shrimp caught by fishermen in the coastal area of Pematang Cengal Village, Tanjung Pura, North Sumatra. The study was conducted on June 9-12, 2025, using a quantitative descriptive method. Sampling was carried out through interviews with fishermen and direct observation of the catch. The results of the study found 10 species of shrimp consisting of 3 families (Penaeidae, Palaemonidae, and Squillidae) from 2 orders (Decapoda and Stomatopoda). The shrimp species found were Penaeus merguiensis, Penaeus monodon, Palaemonid shrimps, Harpiosquilla raphidea, Parapenaeopsis sculptilis, Penaeus indicus, Metapenaeus affinis, Litopanaeus vannamei, Macrobrachium rosenbergii, and Metapeneus brevicornis. Morphometric measurements showed different variations between shrimp species. Based on total length, the size of shrimp caught in the Pematang Cengal Village Coastal Area is relatively stable and falls into the medium-sized category. This research provides important information regarding shrimp species diversity in the Pematang Cengal Village coastal area and can serve as a starting point for shrimp resource conservation in the area.
Antibacterial Activity Test of Ethanol Extract of Fiddlehead (Diplazium esculentum) Agains the Growth of Staphylococcus aureus and Escherichia coli Bacteria in Vitro: Uji Aktivitas Antibakteri Ekstrak Etanol Pakis Sayur (Diplazium esculentum) terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli secara in Vitro Saragih, Hana Dipa Christine; Agustrina, Rochmah; Farisi, Salman; Sumardi
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Infectious diseases are a significant public health issue in both developed and developing countries. Infectious diseases are caused by the bacteria Staphylococcus aureus and Escherichia coli, which cause high rates of morbidity and mortality. Treatment of infectious diseases caused by pathogenic bacteria generally uses antibiotics, but the continuous use of antibiotics can have side effects such as the occurrence of antibiotic resistance.  Diplazium esculentum is one of the medicinal plants that is often used to treat infectious diseases.  The content of secondary metabolite compounds contained in D. esculentum includes saponins, steroids, alkaloids, tannins, glycosides, triterpenoids, phenols, flavones, and flavonoids.  This study aims to determine the effect of bacteriostatic activity of ethanol extract of D. esculentum and its effective concentration in inhibiting the growth of pathogens S. aureus and E. coli.  The study was conducted using a Randomised Complete Block Design (RAKL) with four concentrations of ethanol extract of vegetable fern, namely 20%, 33%, 43%, and 50%.  As a positive control, the antibiotic chloramphenicol was used and the negative control used sterile distilled water.  Data in the form of inhibition zone diameter formed from each treatment were analysed using Kruskal Wallis non-parametric test and continued with Mann Whitney Post Hoc test.  The results of the study showed that the concentration of ethanol extract of D. esculentum showed bacteriostatic activity against S. aureus bacteria at concentrations of 43% and 50% with inhibition zones of 6.52 mm and 5.55 mm, as well as E. coli bacteria at concentrations of 43% and 50% with inhibition zones of 5.11 mm and 4.37 mm, but was not effective in inhibiting the growth of S. aureus and E. coli bacteria.
Tissue Culture of Purple Sweet Potato Using Murashige and Skoog (MS) Medium Based on Benzyl Amino Purine (BAP) : Kultur Jaringan Ubi Jalar Ungu Menggunakan Media Murashige dan Skoog (MS) Berbasis Benzyl Amino Purine BAP Nurul Fajriyah, Walak; Syarifah Ainy, Noer; Hadi, Nestiyanto
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Purple sweet potato (Ipomoea batatas L.) is one of the local food sources rich in anthocyanins and has great potential to be developed through tissue culture techniques. This study aimed to determine the effect of Benzyl Amino Purine (BAP) on the growth of purple sweet potato explants cultured in vitro using Murashige and Skoog (MS) medium. The experiment was conducted using a Completely Randomized Design (CRD) with four BAP concentration treatments (0, 2.5, 3.5, and 5.0 mg/L), each replicated five times, resulting in 20 experimental units. The explants used were stem segments of purple sweet potato (Ipomoea batatas L.). The explants were then cultured on solid Murashige and Skoog (MS) medium containing BAP for 8 weeks. Observed parameters included plant height, callus height, and the percentage of explants forming callus. The results showed that the 3.5 mg/L BAP treatment (N2) produced the best growth, with an average plant height of 2.7 mm, callus height of 1.6 mm, and 100% callus formation. Statistical analysis indicated that BAP application had a significant effect on all observed parameters. Therefore, a concentration of 3.5 mg/L BAP is recommended as the optimal treatment for in vitro propagation of purple sweet potato.
Mortality and Morphological Changes in Aedes aegypti Larvae by Bacillus thuringiensis and Serratia marcescens: Mortalitas dan Perubahan Morfologi Larva Aedes aegypti oleh Bacillus thuringiensis dan Serratia marcescens Yulia Sari, Umi; Rosa, Emantis; Handayani, Kusuma; Busman, Hendri
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

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Abstract

The use of biolarvicides is an alternative biological agent to replace synthetic insecticides, namely entomopathogenic bacteria. The purpose of this study was to determine the potential of Bacillus thuringiensis and Serratia marcescens extracts on mortality and morphological changes in Aedes aegypti larvae. This study was a 2-factor experimental study using a Randomized Block Design (RBD). The first factor, the type of entomopathogenic bacteria, namely B. thuringiensis and S. marcescens. The second factor, the concentration of bacterial extracts (60 mL, 70 mL, 80 mL), negative control (sterile distilled water). Mortality data were processed using ANOVA at the 5% level. The results showed that the administration of B. thuringiensis and S. marcescens extracts caused mortality and morphological changes in Ae. aegypti larvae. B. thuringiensis at concentrations of 60 mL, 70 mL, and 80 mL caused 100% larval mortality, while S. marcescens at concentrations of 60 mL resulted in 70%, 53%, and 50% at concentrations of 70 mL and 80 mL, respectively. Morphological changes in larvae were indicated by changes in color, texture, and damage to the digestive system. It was concluded that B. thuringiensis has the highest potential as an entomopathogenic agent compared to S. marcescens in causing mortality and morphological changes in Ae. aegypti larvae.

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