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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
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Study on the Role of Interleukin-2 and Human Cytomegalovirus in Cases of Recurrent Spontaneous Abortion of Women in Wasit Province Kadhum J. Gatti1, Suhad F. Hasson1, Asawer K. Alsadoon1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.155

Abstract

Recurrent spontaneous abortions (RSA) is the loss of pregnancy for two consecutive times at least before the arrival of the fetus for 24 weeks gestation. This study was conducted to investigate the role of IL-2 and human cytomegalovirus in recurrent spontaneous abortions women in Wasit province. to conduct immunological studies and viral by 90 women who suffer from abortion frequently (At least two abortions) and two sets of control (pregnant and healthy women), The average age of the groups ranged between (17-45) years, the results showed a high significant increase (P <0.001) at the level of IL-- 2 patients compared with pregnant women and healthy women, according to different age groups. And also it showed the results of a study of opposites immune IgM, IgG-virus human cytomegalovirus rise significantly (P <0.05) for patients when compared with pregnant women and healthy women In the cases of chronic and acute infection .
Single Nucleotide Polymorphism of Follicle Stimulating Hormone Receptor Gene in Iraqi Infertile Men Randa Rasool Ghmayes1, Abdul Kareem A.Alkazaz1, Ahmed Adnan A.Rasool2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.156

Abstract

The study was directed to determine Single Nucleotide Polymorphism (rs6166 A>G) of follicle stimulating hormone receptor gene and their association as a risk factor with male infertility in Iraqi sterile patient. In the present study after seminal and serum analysis 50 patient (25 Azoospermia and 25 oligozoospermia) and 50 healthy fertile control were joined. The samples were collected from the Biotechnology Department/college of science/Baghdad university and Kamal Al-Samarie IVF Hospital, Baghdad, Iraq. through the period of two months from November 2018 to January 2019. The SNP (rs 6166) determination was carried out by using real-time polymerase chain reaction (qPCR) of blood samples. The difference in the mean of genotype showed a significantly different (p<0.05) in infertile patients group likened to corresponding means infertile control group. Survey of follicle stimulating hormone receptor gene SNP genotypes and allele frequencies in Azoospermia and oligozoospermia patient groups with the control group, showed that there was a significant variation in the heterozygous (AG) and homozygous mutant (GG) genotype frequencies in (rs 6166). It concluded that this SNP may have a role in an Azoospermia and oligozoospermia Iraqi patients complaining from idiopathic infertility.
Follicle –Stimulating Hormone Receptor Polymorphisms in Iraqi Women with Primary Amenorrhea Rawan Ahmed Nijeeb1, Abdul Kareem A.Alkazaz1, Ruqayah Gheyath Yaseen2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.157

Abstract

The study was carried to determine Single Nucleotide Polymorphism (rs6165) of Follicle Stimulating Hormone Receptor (FSHR) gene in blood samples of 62 Iraqi women with primary amenorrhea and 40 healthy control females. The research included chromosomal study and serum analysis of 62 patient samples. The samples were collected from Educational laboratories, City of Medicine, Baghdad and Biotechnology Dept. College of Science- Baghdad University through the period from October 2018 to March 2019. The determinations of SNP (rs6165) were carried out by real-time PCR. Results of rs6165 genotyping showed significant variations between PA patients and controls, Inspecting FSHR gene genotypes and allele frequencies in PA patients groups with the control group, revealed that there was significant variation in the heterozygous (AG) and homozygous mutant type(AA) genotype frequencies in (rs6165). the SNP of target gene may have a role in PA patients complaining from idiopathic puberty problems.
Study Effect of Clarithromycin drug on Cryptosporidium Parvum and Efficiency of ELISA Technique in Diagnosis Comparison with Some Traditional Methods in vitro Hussein A. Kadhum1, Shaimaa A. Shlash2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.158

Abstract

This research aimed to study effect of Clarithromycin drug on Cryptosporidium Parvum and efficiency of ELISA technique in diagnosis comparison with some traditional methods in vitro. Samples collected from four different sources water were 400 liters:100 liters tap water provider by Al-Kufa District River,100 liters house tank water,100 liters from sea of Al-Najaf and 100 liters stagnant water during period from October 2018 to April 2019.Examined samples by three laboratory methods: Modified Ziehl-Neelson stain, Flotation by saturated sugar solution method and ELISA to detecting from Cryptosporidium oocysts in the water as used Clarithromycin drug effective against C.parvum in vitro. Capability test was performed by impregnation of isolate on cell monolayers and determination the parasite counted after 48 h from the incubation at 37°C. Differ concentrations from Clarithromycin were 4, 8, 16 and 32 mg/L respectively compared with control group.Results of the current study showed that oocysts were found in 182 from 400 liters 45.5% as showed that height oocysts in stagnant water then followed Sea of Al-Najaf were 55% and 45% respectively, height percentage of oocysts were in April and March were 69.09% and 66% while decrease percentage of infection in October was 25%. As showed study results presence significant differences in efficiency of ELISA technique was 83.33% at detection from oocysts compared with Flotation by saturated sugar solution and Modified Ziehl-Neelson stain methods were 66.66% and 61.11% respectively. Shown results this study that Clarithromycin drug in concentrations of 16 and 32 mg/L caused decrease in number oocysts was 17.3±3.5 and 15.4±3.9, sporozoites counts was 17.1±3.4 and 14.9±4.0 compared with control group was 20.5±2.8.As observed significance differences (P?0.05) in the mean numbers of oocysts and sporozoites in three replicate plates after 48 h. These results proved that Clarithromycin have effectiveness in inhibition C.parvum in vitro
Indirect Method for Determination of Paracetamol in Pure and Pharmaceutical Drugs using CFIA with Homemade Ayah 3SBGRX3-3D Solar Cell Microphotometer Analyzer Nagam S. Turkey Al-Awadie1, Marwah A. Kadhim Al-banaa2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.159

Abstract

A method is described for the determination of paracetamol(based on the formation of a orange-reddish color species by reduction of Fe(III) to Fe(II) by paracetamol and then reacted with 1,10-phenanthroline) in the range 0.001-0.09mMol.L-1 (r =0.9801, r2 = 0.9606 , and R2% = 96.06) with a detection limit (LOD) 21.77ng/160?L(3SB)(S/N=3) and with RSD% for 0.01 and 0.07mMol.L-1 less than 1 % (n=6). The method was applied successfully for determination of paracetamol in pharmaceuticals formulation. Analysis of drugs used the standard additions method via the individual t-test. The results showed a significant., difference between the quoted..value of each company. with calculated t-test at 95% confidence(?=0.05) from new method.
Expression and Conservation of rol-genes in Rue, Ruta graveolens L., Plants Regeneration from Hairy Roots Al-Mahdawi M. M.1, Al-Mallah M. K2 & Al-Ne’ma Q. Sh.3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.160

Abstract

In this investigation hairy roots were induced on leave petioles of Ruta graveolens L. plants. These roots were white in color and negative geotropism. Paper electrophoresis of hairy roots extract resulted in the separation of black spots of agropine . These transformed roots were cured from Agrobacterium rhizogenes, some cultures of these roots were directly produced shoots, and other was orientated to produce callus. Subsequently this callus was capable to produce numerous regenerates. Both groups of shoots were rooted easily and successfully adapted to field conditions. Evidences of molecular biology assessment, including isolation and amplification(PCR) of DNA of each transformed tissues and plants coupled with DNA electrophoresis data proved the transfer of rol-genes, except rol B , in the regenerated plants.Conclusion: The genetic modified R. graveolens plants possess unique characters due to the conservation of rol-genes in their genomes.
Assessment of Bioactive Resin-Modified Glass Ionomer Restorative as a New CAD/CAM Material. Part I: Marginal Fitness Study Huda A. Abdulla1, Manhal A. Majeed2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.161

Abstract

The objective of this in vitro study was to evaluate and compare the marginal fitness of monolithic crowns fabricated from a newly developed bioactive CAD/CAM resin block and reinforced resin CAD/CAM block pre-and post-cementation with adhesive and self-adhesive resin cements. Bioactive CAD/CAM block were fabricated from ACTIVA BioACTIVE-RESTORATIVE (Pulpdent Corporation, USA) using a clear rectangular Teflon mold. Thirty-two human maxillary first premolar teeth were prepared to receive full crowns then divided into two main groups of 16 teeth each according to the type of block used to fabricate the crowns: Group A: crowns fabricated from the bioactive resin block, Group B: crowns fabricated from reinforced composite block (BRILLIANT Crios, Coltene). Each group was then subdivided into two subgroups according to the type of resin cement used for cementation, Subgroups (A1, B1): RelyX Ultimate cement, Subgroups (A2, B2): ACTIVA BioACTIVE-cement. The prepared teeth were scanned using CEREC Omnicam digital intra-oral and the crowns were then designed using CEREC Premium software (version 4.4.4) and milled using CEREC MC XL milling unit. The marginal gap of each crown was measured before cementation at four points on each tooth surface using a digital microscope at a magnification of 230x. Each crown was then cemented on its respective tooth according to the manufacturers’ instructions of each cement, and the marginal gap was measured again at the same aforementioned points. The results of this study showed that the marginal gap of all groups are below the clinically acceptable limit. Meanwhile, the marginal gap of the crowns fabricated from both block types increased significantly after cementation with both types of cement. Pre-cementation, student’s t-test revealed that there is no statistically significant difference in the marginal gap of crowns fabricated from both block types (p> 0.05). Post-cementation, a statistically highly significant difference was seen between both block types with both types of cement (p<0.01). From the results of this study, the newly developed bioactive resin block seems a promising material for CAD/CAM applications in terms of marginal fitness.
Study Genotoxicity of Ciprofloxacin in white rats Mustafa Ahmed Shihab1, WAJDI Sabeeh Sadek1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.162

Abstract

The genotoxic and cytotoxic effects of ciprofloxacin drug on the bone marrow of white male rats were assessed by the Micronucleus test (MN) in polychromatic Erythrocytes (PCEs). The DNA damage was assessed by the Comet assay technique in bone marrow, liver and spleen. The white male rats were administered by oral gavage of single doses (93.5), (187) mg.kg-1 of body weight with twice a day for 7 consecutive days, while the negative control group was administered distilled water. The results showed a significant increase in the number of micronuclei corresponding to the duration of exposure., there was a change in the percentage of immature erythrocytes in bone marrow. This increased with an increase in treatment duration. As for the number of immature erythrocytes containing micronuclei, there was a significant increase corresponding to the treatment duration. When using the comet assay technique, the values of DNA damage increased in a dose-related manner.
Thirteen New Yeast Strains Isolated from Cancer Patients in Basrah-Iraq by ITS rDNA Sequencing Mustafa A. Aldossary1, Bashar S. Abdulraheem2, Nasir A. Almansour3, Munaff J. Abd Al-Abbas3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.163

Abstract

Yeast isolates were grown on CHR OMagar Candida medium to detect different types of the yeast species, and then the unidentified isolates were diagnosed by using molecular analysis of ITS region. From a total of 54 yeast isolates, 37 isolates appeared as different species of the genus Candida, the rest of species belong to Hanseniaspora, Kazachstania, Kluyveromyces, Magnusiomyces, Pichia and Saccharomyces. 13yeasts species in this study reported as new strains in Gen Bank, also species belong to C. pronicula, H. uvarum, K. exigua, K. marxianus, M. capitatus, Magnusiomyces sp., P. kudriavzevii, P. manshurica and S. bayanus x S. cerevisiae isolated for the first time in Iraq. We concluded that there are new emergent species of the yeasts inhabit the oral cavity of cancer patients undergoing chemotherapy, and the results showed that the molecular method provide a good approach for the identification.
Evaluation of Effectiveness of Boswelliaserrata (Kundur) Gum Extract on Some Biochemical Parameters in Male Rats Induced Chronic Renal Failure (CRF) Ahmed Neema AL-Mosawy1, Saatar Jasim Hatroosh1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.164

Abstract

Objective: The study was to test water extract ofKundurby treatingchronic renal failure that induced by Cadmium chloride CdCl2.Method: CdCl2. at a concentration of 5 mg /kg body. wt./day of rat at chronic level with drinking water for six weeks as animal model.measuring some predicate biomarkers functional common kidney was done serum creatinine and novel biomarker measured KIM-1 and NAGL, Cystatin C, demonstrate that a regular oral intake of CdCl2 solution (in drinking water) for 45 days.Results: There is significant increase of Serum Urea, creatinine, KIM-1, NAGL and Cystatin C, when compared with control group, also indicate there is significant increase in serum biomarkers for kidney injury. After the treatment with water extract of Kundurby different concentrations 10,20, and 30 mg/kg all these Urea, Scr., KIM-1, NAGL and Cystatin C were significantly decrease when compared with CdCl2 group, compared with control group and after the treatment the damages were not found or minimize. At this exposure of CdCl2.Conclusion: The biomarkers KIM-1, NAGL and Cystatin C may be good predictive indicator of chronic renal failure, plant extract may be having a highly effect for treating the renal failure and showed highly response to treatment.

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