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CYP2D6 (G1934A) Gene Polymorphism in Rice Farmers with Long-term Pesticide Exposure, Suphan Buri, Thailand
Tanunchanok Pitaksilp1 , Kampol Ruchiwit2 , Kowit Suwannahong3 , Wanida Pongstaporn4 , Yuttana Sudja
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12173
CYP2D6 G1934A (rs 3892097) gene polymorphism in coding region is leads to catalytic rate of cytochromeP450 enzyme activity toward xenobiotic, including insecticide. Study was aimed to investigate CYP2D6(G1934A) gene polymorphism in rice farmers along with occupational health risks and behaviors; and toevaluate the relationship of gene polymorphisms and serum cholinesterase (SChE) levels. Cross-sectionalstudy was carried out from June 2019 to February 2020. Data had collected from health service programby U-Thong district health promoting hospital. Gathered information concerning of pesticide exposuresfrom 50 rice farmers and 50 control respondents was conducted by questionnaire interviewing. Eachserum sample was determined SChE activity test by paper test and automatic analyzer; and EDTA bloodwas analyzed genotyping by PCR-RFLP. Chi-square was used to analyze on the different of personalinformation; and of polymorphism. Person correlation was evaluated relationship between SChE level andgenotypes. 60% of rice farmers were used pesticide over 10 years. Means of SChE level of both groupswere within reference value and significantly different (p = 0.033). Polymorphisms were included wild type(GG), heterozygote (GA) and homozygote (AA); and were significantly different between rice farmers andcontrol (p = 0.0001). GA genotype in rice farmers was more frequent and AA genotype was not detected.Relationship of polymorphism and SChE level were negative correlated (r = -0.258; p = 0.009). CYP2D6gene polymorphism may useful biomarker for duties assignments in agricultural workers especially riskgroup (GA genotype).
National Health Insurance Participants Satisfaction in General Polyclinic Services at First Level Healthcare Facilities
Thinni Nurul Rochmah1 , Djazuly Chalidyanto1 , Ernawaty1 , Rachmad Suhanda2,3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12174
The need of health services, especially at first level healthcare facilities (FKTP) for the community to accessbasic health services has increased in Indonesia. This condition related to the regulation for the entire citizenin Indonesia to become a National Health Insurance (JKN) participant. Some problems related to the servicesstill occur, such as queues length and non-optimal examination process. This research aimed to analyzepatients’ satisfaction in general polyclinic services differences in FKTP. This was an observational researchwith a cross-sectional study using primary data from both types of FKTP (Puskesmas and Non-Puskesmas)as the healthcare provider in two cities of East Java. The number of research respondents was 219 in 20FKTP. The sampling method used was proportional random sampling technique. Patients’ satisfactionscores obtained from the two types of FKTP were analyzed using independent sample T Test. The resultsobtained was the satisfaction score in Puskesmas and Non-Puskesmas exceeded 95%. Patients’ satisfaction inPuskesmas was significantly higher than Non-Puskesmas (p = 0.01). Out of the six dimensions, dimensionsof service ability and aesthetics in exception, the other four dimensions had significant differences. Theresults provide recommendation that FKTP should improve their services from various dimensions.
Identification of DNA Corpse in Ordo Diptera Familia Calliphoridae Larvae in CSF1PO, TH01, and TPOX Locus Using Polymerase Chain Reaction (PCR) Methods
Tutik Purwanti1 , Ahmad Yudianto1 , Mochammad Soekry Erfan Kusuma1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12175
Introduction: Biomolecular techniques using DNA are known to have high accuracy in the identification ofdecaying bodies. In the process of identification, larvae found in corpses that have undergone decay can beused as alternative biological evidence to estimate the time of death.Aim: To identify the transfer of body DNA to the larvae of the fly ordo Diptera family Calliphoridae and theprocess of DNA transfer of decomposed bodies.Method: blood from the postmortem corpse was taken as a control, then soft tissue was taken as adecomposition product for the breeding of Dipdo family Calliphoridae larvae. The protocol used is DNAprofiling using the Short Tandem Repeat (STR) locus such as CSF1PO, TH01, and TPOX. The results ofDNA extraction from the postmortem body and DNA extraction results obtained from the skin of the larvaeand the digestive system of the larvae are then matched.Result: The results of this study found that there was DNA transfer in larvae proved by the matching ofDNA at the STR loci on the skin and the digestive system of larvae with the STR locus in blood taken fromthe postmortem bodyConclusion: the presence of decomposed corpse DNA decomposition to the larvae of members of the ordercalled Calliphorideae family which can be detected through the results of nuclear DNA visualization at theCSF1PO, TPOX, and TH01 loci using the PCR method
Children Death with Child Abuse: A Case Report at Bhayangkara Hospital, Kediri Indonesia
Mochammad Soekry Erfan Kusuma1 , Tutik Purwanti2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12176
Background: Child abuse has negative impact for children including emotional performance, mentaldysfunction, and decrease intelligence.Case report: A boy went to the emergency room at Bhayangkara Kediri Hospital. There was swelling on theleft head, expanding on the forehead and, bruising on the left ear. Also found signs of sodomy marks in theform of abrasions around the anus. There are also bruises on the back and neck of the end.Discussion: For a definitive diagnosis, the results of the CT scan were that the patient obtained extensivecerebral contusion and caused the deviation of the midline structure to the right as far as ± 0.9 cm withsubgaleal hematoma and the dilation of cerebral blood vessels.Conclusion: The body’s exact death is expected to blunt force in the head, resulting in a skull fracture andbleeding of the brain.
Effecttiveness of WhatsApp Group on Breastfeeding Practices
Wahidah Sukriani 1,Greiny Arisani 1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12178
Background: Breastmilk is the ideal food for newborn, but in the first days of birth are vulnerable for babiesto be given prelactal food by mother and families. The Successful of exclusive breastfeeding related tovarious factors including media exposure. Mothers who are exposed to the media have more opportunitiesto give exclusive breastfeeding. WhatsApp in a new trend in communication method for giving informationto the public.Objective: This study aims to determine the Effectiveness of WhatsApp Group Towards Pregnant womanin The Practice of First Week Breastfeeding in PalangkarayaMethods: This study is a quasi-experimental study with posttest only with control group design. Purposivesampling technique was used in this study, consisted 36 respondent who were divided into two groups.Analysis were performed by chi-square test.Result: The results showed the average age of respondents was 26.94 years. Most of the respondents werehousewives or unemployed (63.9%), had high and middle education (88.9%) and had high family income(77.7%). Based on statistical test results, WhatsApp Group is effectively used to improve breastfeedingbehavior of mothers in the first week after birth (p <0.05).Conclusion: Health workers are expected to be able to use WhatsApp Group as a health education media onbreastfeeding to increase the scope of exclusive breast milk.
The Effect of the Blood Cupping Therapy on High Density Lipoprotein (HDL) and Low Density Lipoprotein (LDL) in Hypercholesterol Patients
Wahyudi Widada1 , Teddy Ontoseno2 , Bambang Purwanto3 , Suhartati4 , Imam Subadi5 , Hari Basuki Noto
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12179
Introduction: Hypercholesterol is a high cholesterol level in the blood. Blood cupping therapy is a techniqueof excreting metabolic waste in the blood through the skin. The study aimed to measure any effect of bloodcupping therapy to HDL and LDL cholesterol. Method: It was a Quasi-Experimental study using humansas research subjects. Non random consecutive sampling. Patiens with hypercholesterol after 12 hours offasting were treated with blood cupping, 9 points. Research subjects were 51 men divided into three groups,the cupping group only consisted of 17 men, the drug group without cupping consisted of 17 men, andthe cupping and drug consisted 17 men. Collecting data from third of the groups as pre and post data.The data were analyzed by Mann Whitney, Wilcoxon and Kruskal Wallis. The research took place at theLaboratory of Biochemistry, Universitas Jember. Results: Measurement of HDL cholesterol on the cuppinggroup was pretest=33.03±3.73; posttest=37.58±6.54; p=0.000. The drug group was pretest=33.40±3.18;posttest=33.56±3.50; p=0.788. The cupping and drug group was pretest=34.32±3.38; posttest=37.61±2.01;p=0.002. Kruskal Wallis test on pretest group was 0.534 and on posttest group was 0.002. Measurement ofLDL cholesterol on the cupping group was pretest=154.70±39.68; posttest=123.89±41.86; p=0.000. Thedrug group was pretest=151.24±44.17; posttest=151.24±44.17; p=0.019. The cupping and drug group waspretest=147.48±62.66; posttest=105.57±57.94; p=0.001. Kruskal Wallis test on pretest group was 0.439and on posttest group was 0.082. This means that there was no difference in the average pretest and posttestLDL levels in the cupping, drug, cupping and drug groups. Conclusion: The intervention of blood cuppingtherapy can increase HDL cholesterol level, and reduce LDL cholesterol level. Further research needs to bedone to measure the potential prevention of atherosclerosis.
Impact of High Fidelity Simulation Training on the Quality of Cardiopulmonary Resuscitation Performance among Emergency Medical Services (EMS) Professionals in Pune, India
Anand Hinduja1 , Parag Rishipathak2 , Shrimathy Vijayaraghavan3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12180
Introduction: The provision of high quality cardio pulmonary resuscitation (CPR) is a key modifiablefactor associated with survival in cardiac arrest. Management of a patient with cardiac arrest is a dynamicand high-pressure challenge requiring critical decision making in a short period of time. Numerous studiesemphasize that quality of chest compressions directly influences survival rate from cardiac arrest. Hence,improving CPR quality is of paramount importance. This can be achieved by accurate measurement ofvarious CPR metrics and provision of real time feedback to the rescuer.Objective: The study aims to assess impact of high fidelity simulation training on quality of CPR performanceof EMS Professionals.Methods: A high fidelity manikin was utilized to create four unique healthcare simulation scenarios based oncardiac arrest. The manikin software was capable of capturing real-time CPR data and measured parameterslike chest compression depth (CCD), chest compression rate (CCR), chest recoil (CR) and chest compressionfraction (CCF). The participants were initially taught the assessment and management of cardiac arrest. Theparticipants underwent a pre-intervention test conducted on high fidelity manikin. The CPR metrics wererecorded during the session. The participants were then imparted training with real time CPR feedback for aperiod of two months. This was followed by a post-intervention test and the CPR metrics of both tests werecompared.Discussion: The participants showed improvement in chest compression depth and chest compression ratebut the chest recoil frequency and chest compression fraction decreased after the two-month interventionperiod. The overemphasis on achieving target depth and rate led to ignorance of the other two parametersaffecting overall quality of CPR.Conclusion: Maintaining adequate attention on all aspects of CPR metrics is a complex challenge even fortrained professionals. Utilizing technology for real-time feedback is a reliable method to gauge improvementin quality of CPR during training sessions of EMS professionals.
Relationship of Smartphone Usage and Academic Performance among Undergraduate students – A Systematic Review
Ancy Albert1 ; Nancy Netto1 ; Neelkant Pawar1 ; Fenilkumar Patel1 , Sharadha Ramesh2 ; Manisha Mistr
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12181
Background: Smartphones have become the new normal of 21st century. With growing need to becomedigitally competent each individual is trying to own one smartphone or a similar gadget.Methodology: PRISMA guidelines were used to conduct a systematic review of Literature. 98 researcharticles were reviewed by the team using specific key words. They were scrutinized for their eligibility toget selected for further in-depth review. Articles were selected based on the appropriateness of the titles,abstracts, and results. After a thorough check only 06 reviews were analyzed and paraphrased.Results: smartphone addiction is the major reason for poor academic performance of the students.Conclusion: The smartphones must be assessed for their usability and suitability to be used by the students.The phones maybe of a great help to a student if he sees learning opportunities in it.
A Study to Assess the Effectiveness of Mindful Meditation on Health and Psychological Adjustment Faced by Nursing Students in Selected Colleges of Pune City
Anu Alexander1 , Ms. Feba Josemon1 , Shreeti Gahare1 , Neha Jadhav1 , Sonopant G Joshi2 , Ranjana Ch
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12182
Nursing students experiences more stress in their early academic period. Psychological adjustment becomesdifficult for them due to many challenges. It is assumed that mindful meditation will helpful for them tocope up with adjustment problems. A study to assess the effectiveness of mindful meditation on healthand psychological adjustment faced by first year B.Sc nursing students in selected nursing college of Punecity. Purpose of the study to assess the level of health and psychological adjustment and evaluation ofmindful meditation on their adjustment. Pre-experimental study was conducted to find out the adjustmentproblems. The research design was one group pre-test post-test experimental design. The sample size was40. Purposive sampling technique was used to select the students. The result showed that first year B ScNursing students faces multiple adjustment problems related to their health and psychological adjustments.Mindful Meditation in early academic life was proved very useful in adjusting the new world of academicenvironment.
Health and Lifestyle Practices of Under Graduate Students Studying in Selected Nursing College
Anagha.M1 , Ritu Gaikwad1 , Ronald Masumba1 , Kraus1 , Asha Varghese1 , Deepak Sethi,2 Merlin Suji3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i4.12183
Life styles of the people is decided as early in early childhood period. School and family practices areessential in deciding healthy and lifestyle practices in adulthood. Sometimes, peer pressure changes theindividuals’ lifestyles. The study aimed to assess the healthy and lifestyles practices of undergraduate studentsstudying in selected nursing college. Methodology: A Non-Experimental Descriptive Research approachused in various nursing colleges in Pune city. The selection of the sample was based on easy geographicalaccessibility, cooperation and availability of samples .The sample size for the study was 96,selected byusing non probability convenience sampling technique who met the inclusion criteria. A self-structuredquestionnaire was developed based on the research problem, to get the information from the samples.Result : As for the nutrition the mean score is 48. 64% of adolescents replied that they will avoid half cookedfood, 56% replied they avoid fast foods and 59% replied they prefer healthy eating.91% replied that theydon’t have any problem in paying attention. 75% doesn’t have any problem for sleep. 62% replied that theyhave troubles in relaxing and 44 % replied they usually get into the arguments. 55% involved in activitieslike sports, games and dance. 70% feels healthy and 68% have complaints of stomachaches, headaches, orother aches and pains. 96% have no problems in handling life. Around 93 % satisfied with their family .Around 93% have the support with the family and friends. 57.29 % replied that they don’t have computer intheir home. 61.46 % students live with their parents. 96.88% has personal mobile. 73% regularly visit socialnetworking sites. 96% of adolescents wash their hands before eating foods and 83% wash their hands withsoap or hand wash. 16.67% have habits of cigarettes/chewing Pan/Guthka but 945 aware about the harmfuleffects of smoking. 13% replied they tried alcohol but they believe that 41% of their friends has alcoholdrinking habits. 86% students feel getting good score in college is important to family. 94% feel safe incollege and only 4% says that their behavior influenced by peer pressure. Others 96% doesn’t agree with it.Conclusion: Diet practices, concentration in work , family connectedness scored less compared to othercomponents. Very few students have the habits of alcohol, smoking etc. Personal hygiene was practiced wellin adolescents. Decision is taken by themselves more than the peer pressure. Majority of the adolescentsknows the important of their education and they feel safe and good in college.