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Indian Journal of Forensic Medicine & Toxicology
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Articles 5,497 Documents
Assessment the effects of Mastic Gum Resin, Lawsonia Inermis and Quercus Brantii on Cutaneous Wound Healing in BALB/c Mice Kwestan Najm Ali1, , Hardi Fattah Marif1 , Hana Sherzad Raoof1 , Snur M. A. Hassan2 , Dereen Omer Ra
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13705

Abstract

The request for more efficient and lower-cost therapeutic methods for wound healing remains a challengefor modern medicine. The goals of this investigation were to recognize and look at the impacts of MasticGum Resin, Lawsonia Inermis, and Quercus Brantii in wound healing by using histopathological study andblood parameters in BALB/c Mice. Mice were comprised into four groups: Control negative group (n=10),mice were not treated with plant suspension only applied with normal saline; Treatment group I, mice wereapplied by Mastic Gum Resin (MGR) (n=10), Treatment group II, mice were applied with the QuercusBrantii (n=10), and the last group, Treatment III which were applied with Lawsonia Inermis (LI) (n=10).One ml for each suspension of Mastic Gum Resin (MGR), Quercus Brantii (QB), and Lawsonia Inermis(LI) was applied to the wound directly without suturing for 4 days/week for about 3 weeks. Wound healingeffects were evaluated by utilizing the hematological profile for each group with the histopathological study.The cutaneous wound in Mastic Gum Resin and Quercus Brantii treated groups were more effective inprogressing wound healing than Lawsonia Inermis treated group regarding histological changes at day 8 andday 18, respectively and blood parameters at day 21.
Clinical and Histopathological study of black and red grape seed extracts (Vitis Vinifera) effects on the Albino Mice Snur M. A. Hassan1 , Shno N. Hassan2 , 3 Nian N.N.Maarof
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13706

Abstract

Grape seed extract (GSE) is a complex mixture of several compounds, mostly represented by polyphenolsand phenolic acids. The goal of the pilot study was to illustrate the safe dose of GSE in mice model andto assess toxicity that may be initiated by the different concentrations of this plant. Forty-two mice weredivided equally into 7 groups; groups 1 attended as control, were only received water, whereas animals ofgroups 2, 3, 4 were treated orally with 200, 400, and 800 mg/kg b.w. of black grape seed extract respectively,and the remaining groups including 5, 6, and 7 were treated orally with 200, 400, and 800 mg/kg b.w. ofred grape seed extract respectively. The animals were observed daily for any sign revealing for activityalterations and toxicity along with their body weight measurement during the experiment for 21 days withhistopathological examination. The results gained from this pilot study were recording that the 200 and 400mg/kg b.w. doses of GSE were safe compared to the 800 mg/kg b.w.in both black and red grape seed extractsbecause the higher dose led to a reduction in body weight gain and produce changes in the mentioned organs.
Detection of Pork in Canned Meat Products by using DNA-based Methods Alaa Tariq Abdulwahid
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13707

Abstract

Adulteration of meat products became a matter of great concerns of religious, economical, legal and hygienicaspects. Canned meat is one of the most favorable in a lot of countries, which makes it prone to adulteration.The objective of the current study was to identify pork in canned meat for the presence of adulteration incommercial market of Basrah city/Iraq. Thirty canned meat were collected from commercial market. Thepossibility of a species mixture was tested with polymerase chain reaction (PCR), targeting pork (290bp).Analysis of canned meat revealed negativity results of all samples to pork meat. In conclusion, samplesanalysed in the current study showed that there was no adulteration by mixing pork meat in canned meatproducts due to absent production of pork flesh for religion and hygienic aspects. Beef and mutton fleshmight be replaced in chicken, horse, and donkey flesh for economic reason.
Effects of Adding Dry Mulberry Leaves (Morusalba) in the Concentrate Diet on Digestibility and Some Blood Parameters in Female Goats Jassim E. Q. Al-Musawi1 , Wathiq A.H. AL-Jbory2
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13708

Abstract

This experimental study was conducted at the animal farm of the College of Veterinary Medicine ofBaghdad University during the period 15/3/2019 until 30/4/2019. Fourteen female local breed goats, ataged between 1.5–2 years and range weight 23–27kg.The animals were divided into two equal groups asfollowing G1(control) was fed on concentrate diet 2% B.W and alfa - alfa hay .G2 group was fed the samediet and 2% grinding Mulberry leaves of concentrate diet .water was offered freely. For evaluation someanimal performance. Bodyweight, some nutrients digestibility, blood picture and biochemical parameters.Results revealed that an excellent improvement was observed in body weight and nutrients digestibilitywere significantly (P<0.05). Similar effects in blood biochemical parameters (total protein and urea),while no differences were determined in triglycerides and cholesterol. other blood parameters showed thathemoglobin (Hb) and packed cell volume (PCV) which were significantly higher (P <0.05) and furtherimproved in erythrocytes, leucocytes, lymphocytes and neutrophils in the group received Mulberry leavesthan control groups.
In Vitro Production of Ovine Embryo in Non-Breeding Season A. A. Omar1 , M. N. Ahmed2 , Audai Sabah Asker3 , A. F. Majeed1 , T.M. Faraj4 , S.J. Rejah4
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13709

Abstract

This study was undertaken to show the effect of follicular size and culture media on the results of IVFand IVEP in sheep. A hundred ovaries of slaughtered ewes were taken during non-breeding season. Thetotal number of follicles were 256, while the number of recovered oocytes were 189. The recovery rateof oocyte from large and small follicles were 85.8% and 64.7% respectively. Only grades A and B oocytehave been used with a total number of 148 oocytes. Maturation and fertilization rates in different media;MEM, RPMI1640, DMEM low glucose and DMEM high glucose were 64.4%, 51.2%, 32.4% and 26.6% formaturation rate and 66.6%, 55%, 50% and 37.5% for fertilization rate respectively. There was a statisticaldifference (p?0.05) in maturation and fertilization rate between different media; the total fertilization andcleavage rate was 56.7% and 70% respectively. The numbers of morula and blastocyst stage were 40% and35% respectively. There was a statistical difference (p?0.05) in morula and blastocyst production in differentmedia. It was concluded that abattoir was a good source of oocyte recovery, and the large follicle give thebest morula and blastocyst stage. MEM was a good diluent of semen. mMEM give the best result as culturedmedia as compared with the other media used in this study.
Isolation and Identification of Lactic Acid Bacteria from Buffalo’s Raw Milk in Basrah Province by Sequencing the 16S rRNA. Hiba A .Nasear1 , Samar S. Ghazi1 , Alaa T. Abdulwahid2
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13710

Abstract

Milk is a nutrient substance that screted from mammary glands of mammals. Lactic acid bacteria (LAB)of raw milk are natural microflora and might be starter cultures for milk fermentation. This study aimed toisolate LABs from raw buffalo’s milk and identify them by 16S rRNA sequencing for possible uses in themanufacture of food product., A total of 30 buffalo’s raw milk samples were collected from different areasof Basrah province (Shut-Alarab,Al-Zubair,Al-Qurna, Basrah conter and Abi-Elkhasib through the periodextended from October to December 2019 to isolate the LABs using conventional bacteriology test andidentify them by 16S rRNA sequencing using Polymerase Chain Reaction assay (DNA-based method).DNA extraction was carried out in duplicate for all raw milk samples. The results showed buffalo’s milkraw showed positive result for bacteriological test (Gram positive-catalase negative)and for the presenceof the 16S rRNA gene. There was no significant difference among ages, months, and area of the currentstudy. Buffalo’s raw milk at >4-8 year of age showed a higher ratio of LABs (71.43%) compared with thatof buffalo at >1-4 year of age (47.83%). In addition, the highest ratio of LABs in buffalo’s raw milk was inOctober (66.6%) and the lowest one was in November (36.3%). Furthermore, the highest ratio of LABs inbuffalo’s raw milk was in Abi- Elkhasib (100 %) and the lowest one was in AL-Zubair (20%). In conclusionLactic acid bacteria were found in buffalo’s raw milk. Biochemical and genotypic tests are effectively usedto identify the LABs. All LABs isolates could be survived well in buffalo’s raw milk in any age per year,months, and area of study. These findings suggest the possibility that LABs isolates from buffalo’s raw milkmight use to inhibit pathogenic and spoilage bacteria in the food products and might improve flavour andquality of the food products.
Evaluating the Efficacy of Some Disinfectants, Sterilizers and Detergents Against Streptococcus Pyogenes Isolates from Tonsillitis Patient in Kirkuk City Aylan Arslan Ali1 , Shymaa Hassan Ali2
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13711

Abstract

The aim of the study was to evaluate the inhibitory effectiveness of some disinfectants, sterilizers anddetergents commonly used, including: Dettol (Sabtol( ,Bleach, ethanol alcohol, liquid soap, dishwashingliquid, ActiveX and Sterile Gel 85 on bacterial growth Streptococcus pyogenes, The study includedcollecting (150) swabs from Patients referred to the hospital and from private medical laboratories (agegroups were16-40 years) ,the swabs were taken by (Transport media) containing the prepared agriculturalmedium and transported to the laboratory for the purpose of isolation and diagnosis.The results of the bacterial sensitivity test under study for these disinfectants, sterilizers and detergentsaccording to the type and concentrations of the preparation (50,25,12.5)%, and that alcohol ethanol,hypochlorite sodium and sterile gel(Gel 85)have a high effect on the growth of bacteria Streptococcuspyogenes and with all the concentrations prepared, and while detergents Liquid and liquid soap, dishwashing,had less effect on the bacteria under study, according to the prepared concentrations.
Study the Role of Selenium or Zinc as Organic form on some Antioxidant and Liver Enzymes of Rams Mohannad. K. Aremmt1 , Saad Thabit Jassim Alrawi2 , T.R. Mohammed3
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13712

Abstract

This study was conducted to detect the role of the organic form of Selenium or Zinc that supported withyeast (Saccharomyces cerevisiae) on the liver function and antioxidant enzymes. Twenty-one rams that theiraverage weight of 38±2 kg. The males divided into three groups. The first group (G1) late without treatment,group two (G2) was administrated with (Saccharomyces cerevisiae-Zinc) (0.2 g/kg/ body weight), whilegroup three (G3) was administrated with (Saccharomyces cerevisiae-Selenium) (0.03 g/kg/ body weight).Blood samples were collected from males at zero 45 and 90 days of experiment. The results of AST, ALT andALP showed non-significant differences between different groups. While the activity antioxidant enzymesrecorded a significant improvement, through a significant increased (P?0.05) of Glutathione peroxidase andcatalase concentrations of the group that treated with organic selenium or zinc compare with a control group,from another hand, the treated group with organic selenium has been shown the significant increases (P?0.05)of Glutathione peroxidase compared with the animals that treated zinc at the third period. Whereas, the treatedgroups recoded a significant increment (P?0.05) with the progress of ages. From results concluded that thefeeding selenium and zinc as an organic form that fortified by yeast Saccharomyces cerevisiae significantlyincreases antioxidant enzyme activity and have a protective role to the liver.
Cytotoxic activity of Green Zinc Selenide Nanoparticles Against Hep-G2 Cell Lines Sabah N. Mazhir1 , Kadhim A. Aadim2 , Mohammad M. F. Al-Halbosiy3 ,Nisreen Kh.Abdalameer4
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13713

Abstract

In the current study a new technique between the plasma physics, nanoparticle and Biotechnology,structured(ZnSe) prepared by cold plasma technique using atmospheric pressure plasmajet system, Znse NPs wereprepared from selenium nitrate and zinc metal sheets to reduce selenium toxicity. The structural and opticalproperties were characterized by X-ray diffraction (XRD) and absorbance measurements of ZnSe greenNPs. The liver cancer cell line (hepG2) was exposed to the ZnSe nanoparticles, and The percentage ofcytotoxicity was suppressed after 24, and48 from exposure, induced cytotoxicity is found to be 70.3% inhepG2 after exposed 48h ZnSe green nanoparticle, and the maximum cytotoxicity of the normal cell line(REF) was also examined 42.62% when exposure ZnSe nanoparticles and 16.6% when exposure ZnSe greennanoparticles.
Cytotoxicity of lipopolysaccharide extracted from Salmonella enterica serovar Typhimurium on Breast Cancer Cell Line mcf-7 Wissam Qadry Mutaab Alqaissy1 , Aziz L.M.2 , Abdul-Rahman Ibrahim A.3
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13714

Abstract

Lipopolysaccharide was selected to investigate its effect on cell line of breast cancer in vitro, thereforelipopolysaccharide was extract from Salmonella enterica serovar Typhimurium followed by evaluation of itscytotoxicity. The experiment was divided into three parts. The first part included isolation and identificationof S. enterica serovar Typhimurium from 302 samples included 201diarrheic patient samples and 101 foodsamples of poultry meat and as well as the isolates identification on molecular level through used of threegenes (invA, STM4497 and Stn) genes detected using the technique of PCR, the results shown there wereseven bacterial isolates diagnosed as S. enterica serovar Typhimurium. The second part of the experimentwas extract of LPS from 10.66 g of dry weight of bacteria by hot phenol method. The concentration of LPSin phenolic phase was 435.57?g/ml while in aqueous phase was 381.76 ?g/ml. The LPS molecular weightwas estimate which was equivalent to 93325 for phenolic and 71614 Dalton for aqueous phases. The thirdpart of experiment was to study the effect of cytotoxicity in cell line of breast cancer by used differentconcentrations of lipopolysaccharide. Lipopolysaccharide showed cytotoxicity on the breast cancer cell linemcf-7 in both phenolic and aqueous phases of LPS. The results showed the highest rate of inhibition ofbreast cancer cells at the concentration of 350 ?g/ml by 61.67% in the phenolic phase while the rate ofinhibition was 53.33% in the same concentration of the aqueous phase.