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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 5 Documents

Search results for , issue "Vol 12 No 4 (2016)" : 5 Documents clear

Identifikasi Pepper vein yellows virus yang Berasosiasi dengan Penyakit Yellow Vein Banding pada Tanaman Mentimun di Bali I Dewa Nyoman Nyana; M. Tinny Lestariningsih; Ni Nengah Putri Adnyani; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellowing vein banding disease has been reported infecting cucurbit plants in Bali since 2014. Similar vein banding symptom on chilli pepper was observed previously, and early diagnosis indicated infection of Polerovirus. The objective of this research was to confirm the presence of Polerovirus infection on cucumber plant showing yellow vein banding symptom in Bali. Reverse transcription polymerase chain reaction – based detection method was conducted using specific primer pairs PeVYV-CP-F-BamH1/ PeVYV-CP-R-Pst1followed by sequencing and nucleotide sequence analysis. Specific DNA fragments of ± 650 bp was successfully amplified from field samples. Nucleotide sequence analysis showed that the sequence has the highest similarity > 95% with Pepper vein yellow virus (PeVYV) infecting chili pepper from Indonesia (Bali, and Rembang), Japan, and Greece.

Pengendalian Colletotrichum spp. Terbawa Benih Cabai dengan Paparan Gelombang Mikro Lilih Naelun Najah; Mohamad Rahmad Suhartanto; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Seed treatment using microwave has been reported as an effective and efficient method to control seed borne pathogens of chili pepper. The purpose of this research was to evaluate the effectiveness of microwave irradiation treatment to suppress seedborne Colletotrichum spp. while maintaining physiological quality of chili’s seed. First experiment was aimed to select the best condition for seed germination, and was done in completely randomized design with three levels of water content (4.31%, 6.33%, and 8.25%). The second experiment was aimed to determine the best condition for disease suppression, and was done in completely randomized design with different levels of microwave irradiation duration (0, 10, 20, 30, 40, and 50 seconds) using the best seed water content level from the first experiment. Application of systemic fungicide with benomyl as active ingredient (0.5 g L-1) was done as check treatment. Four major species of Colletotrichum was found from chili’s seed, i.e. C. acutatum, C. capsici, C. gloeosporioides, and Colletotrichum sp. with C. acutatum as the predominant species. The best water content level for microwave treatment was 4.31%. The most effective treatment was microwave irradiation duration for 40 seconds with efficacy rate of 64.5% for controlling seedborne C. acutatum on chili pepper.

Komparasi Metode Isolasi DNA Patogen Antraknosa dan Bulai untuk Deteksi PCR Ade Syahputra; Kikin Hamzah Mutaqin; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Polymerase chain reaction (PCR) is an important tool for detection, identification and monitoring of quarantine pests in Indonesia. DNA isolation method from target organism is an important step to provide adequate DNA template for performing PCR. Objective of the research was to compare conventional, commercial kit, FTA-card and its modification methods of DNA isolation to be used in PCR detection for Colletotrichum acutatum and Peronosclerospora sorghi from chili and maize, respectively. DNA obtained from various isolation methods were measured using UV-vis nanodrop-spectrophotometry. DNA amplification was performed using DNA concentration of 15 ng µL-1 from each isolation method with gradual primer concentrations of 0.4; 0.6; 0.8; and 1.0 mM. The highest concentration of DNA was achieved with conventional methods for C. acutatum from pure culture and P. sorghi from maize leaf. Best DNA purity was obtained from isolation method using commercial kit for C. acutatum from infected fruit (1.94) and from conventional method for C. acutatum from pure culture (1.91). The highest total yield of isolated DNA was achieved by modified FTA-card for C. acutatum from pure culture. In general DNA amplification using various primer concentration gave positive results although DNA bands intensity was varied from faint to very bright. Furthermore PCR optimization using the best primer concentration from previous reaction showed that all DNA templates resulted in thick and bright DNA bands.

Cendawan Endofit yang Potensial Meningkatkan Ketahanan Cabai Merah terhadap Penyakit Layu Bakteri Ana Feronika Cindra Irawati; Yudi Sastro; Sulastri Sulastri; Maggy Tenawidjaja Suhartono; Kikin Hamzah Mutaqin; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Ralstonia solanacearum has been known to cause bacterial wilt disease on chili pepper. Despite many reports on the potential use of endophytic fungi to induce plant resistance, its utilization to suppress bacterial wilt disease of chili has not been widely reported. The aims of this research was to screen potential endophytic fungi that may increase chili resistance against bacterial wilt disease. Selection of endophytic fungi was done using in vivo and antibiosis test. Strains of fungi were considered the most potent in suppressing the development of bacterial wilt in chilli were identified. Ten out of 62 isolates of endophytic fungi gave the highest suppression on chilli’s bacterial wilt disease. Most strains of endophytic fungi were able to suppress the development of bacterial wilt disease, but not always positively correlated to the vegetative and generative growth of chilli. Based on the level of disease intensity and the growth of plants were obtained three strains of endophytic fungi that considered potentially suppress the incidence of bacterial wilt disease. The three isolates was identified as Fusarium solani f.sp. phaseoli (AC-2.13 and AC-4.4) and Trichoderma asperellum (AC-3.18) using morphology and molecular characters. Although all three selected isolates were able to suppress bacterial wilt disease in this study, but application of F. solani f.sp. phaseoli should be considered in practical use since it is generally known as the causal agent of root rot disease of beans

Vascular Streak Dieback: Penyakit Baru Tanaman Kakao di Sumatera Barat Jumsu Trisno; Reflin Reflin; Martinius Martinius
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Vascular streak dieback (VSD) symptoms was reported recently in several cacao plantations in West Sumatera. Disease incidence reached 58.82–100% with disease intensity of 24.29–44.71%. In some cases, dead plant was also found. Fungal isolation was performed to identify the agents associated with VSD. Plant samples showing VSD symptoms was collected from 3 locations of cacao production center in West Sumatera, i.e. Limapuluh Kota regency, Padang Pariaman regency, and Padang city. Small pieces of leaf and twig were plated on water agar and potato dextrose agar medium for fungal isolation. Morphology of hifa, basidiocarp, and basidiospora observed from fungi colonies indicated the presence of Ceratobasidium theobromae on infected plant samples. This is the first report on the association of C. theobromae on cacao in West Sumatera.

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