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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 5 Documents

Search results for , issue "Vol. 13 No. 2 (2017)" : 5 Documents clear

Kepekaan Erythricium salmonicolor Penyebab Penyakit Jamur Upas pada Kemiri Sunan terhadap Suhu, Cahaya, dan Fungisida Florina, Dini; Wahyuno, Dono; Siswanto, Siswanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

A typical pink disease symptom appeared on stem of Reutealis trisperma in Bogor. The present study was aimed for confirming the causal pathogen and testing its sensitivity to temperature, light and fungicides. The fungus was identified based on its morphological characteristics. The fungal inoculum was obtained by tissue isolation of infected stem bark. For pathogenicity test, the fungus was inoculated onto sterilized pieces of woody R. trisperma branch, then fastened on the seedlings and incubated in the green house. The effect of temperatures on fungal growth was tested by planting the fungal colony onto potato dextrose agar (PDA) then incubated at 25–34 °C. The sensitivity of the fungus to light was tested by inoculating the fungus into flask with potato dextrose broth (PDB) medium on a shaker (60 rpm) exposing it to white light tube (400 luks) or in a dark condition for 15 days at 25 °C. The obtained fungal colonies were weighed. Efficacy of copper hydroxide and mancozeb against the fungal colony growth was tested by inoculating the fungus onto PDA amended with 100, 200, 300, 400, and 500 ppm, respectively, of tested fungicides. The results showed that the causal pathogen was Erythricium salmonicolor. In the pathogenicity test, cobwebs of E. salmonicolor were formed on inoculated seedlings 3 weeks after inoculation. The optimum temperature for growth of E. salmonicolor was 28 °C. There was no significant effect of light on fungal growth. Mancozeb suppressed colony of E. salmonicolor growth better than copper hydroxide.

Cara Preservasi Fitoplasma dari Jaringan Kacang Tanah Bergejala Sapu untuk Deteksi DNA dengan Teknik PCR Pulogu, Siska Irhamnawati; Mutaqin, Kikin Hamzah; Giyanto, Giyanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Witches‘ broom of peanut caused by phytoplasma is a common disease found in Indonesia. Phytoplasma is able to be detected using polymerase chain reaction (PCR) technique. One of important factor which determine the successful of phytoplasma amplification is the DNA availability from fresh tissues. The research was aimed to evaluate some preservation methods of phytoplasma from infected plant samples. The aspects to be evaluated consisted of time (1, 2, 3, and 4 weeks), temperature (-20 °C, 4 °C, and 25 °C), and preservation medium (1X PGB buffer, 3 M NaCl, CTAB buffer, 70% ethanol, non medium, and FTA-card) for storing the fresh phytoplasma infected samples. Good preservation method will optimize the phytoplasma DNA amplification using PCR standard technique followed by nested-PCR. The results showed that preservation of samples at -20 °C, 4 °C, and 25 °C in CTAB buffer was able to maintain the tissue freshness for 4 weeks and was able to provide the DNA of either quality or quantity sufficiently for PCR detection. PCR standard using a primer pair P1/P7 showed that not all of the preserved DNA of phytoplasma were amplified positively. However, standard PCR followed by nested-PCR using primer pair fU5/rU3 was able to increase the DNA detectability. Preserved samples derived from various medium and stored for 4 weeks gave positive results. This results were in contrary with previous same samples which were detected negatively by standard PCR technique.

Ketahanan Kultivar Pisang Lokal Kalimantan Selatan terhadap Penyakit Bercak Sigatoka (Mycosphaerella sp.) Mariana, Mariana; Rodinah, Rodinah; Budi, Ismed Setya
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Sigatoka of banana caused by Mycospherella sp. is an important disease, since it causes yield losses. Resistant variety is considered a promising approach for sigatoka disease management. This study was aimed to evaluate the resistance of local banana cultivars from South Kalimantan against sigatoka disease. The fungus was isolated from leaves of Kapas cultivar showing severe symptoms of sigatoka disease, onto potato dextrose agar medium. The isolate was then confirmed as Mycosphaerella sp. following Koch’s postulate. Level of resistance is determined based on symptoms development. Resistance of local banana cultivars can be differentiated into susceptible (‘Awak’, ‘Jaranang’, ‘Kapas’, ‘Mas’, and ‘Paikat’), medium resistant (‘Ambon’, ‘Kepok’, ‘Mas Bantan’, ‘Mauli’, and ‘Talas’), and resistant (‘Tarati’). In general, the symptom was visible 2 to 8 days after inoculation. The longer incubation period the more resistant response of banana varieties. Incubation period was negatively correlated to the disease intensity, but it is not correlated with stomatal density.

Galur Isogenik Xanthomonas oryzae pv. oryzae Hasil Penyinaran Ultraviolet dan Potensinya Sebagai Penginduksi Resistensi Padi terhadap Penyakit Hawar Daun Bakteri Yuliani, Fitria; Giyanto, Giyanto; Mutaqin, Kikin Hamzah
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is an important rice disease and may cause 50% plant damage. One approach to manage this disease is using isogenic non-pathogenic strain that can induce plant resistance. This study was conducted to obtain isogenic strains of X. oryzae pv. oryzae through ultraviolet irradiation and evaluate ability of the isolates in inducing plant resistance. Bacteria X. oryzae pv. oryzae were exposed to UV for 3, 5, 10 and 15 min. A total of 99 colonies obtained were selected through pathogenicity tests, followed by confirmation using XOR-F and XOR-R2 primers. As much as 16 isogenic strain isolates were obtained with the ability to produce exopolysaccharides (EPS) ranges between 1.4–9.8 mg mL-1 compared to wild-type 6.0 mg mL-1. Five isogenic strains isolates (M313, M57, M101, M1513 and M1515) caused virulence reduction of 92.8–98.6%. Application of those five isogenic isolates prolonged incubation period of X. oryzae pv. Oryzae, i.e. 6.6–5.4 days compared to the wild-type i.e. 4.8 days after inoculation. Suppression of disease progression was also observed with AUDPC value 615.0-827.5, which was lower than those caused by wild-type and control, i.e. 863.3 and 682.5, respectively. Application of isogenic strain isolates did not affect plant growth but it increased plant productivity. Isogenic strain M101 was the best isolate with an incubation period of 5.7 days after inoculation, having the lowest AUDPC value (615.0), and yielded dry weight of 100 grains higher (2.8 g) than those of the wild-type (2.0 g).

Penyakit Busuk Akar dan Pangkal Batang Tebu di Sumatera Selatan Maryono, Tri; Widiastuti, Ani; Priyatmojo, Achmadi
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root and basal stem rot disease caused by Xylaria sp. is a new diseases in Indonesian sugarcane plantation. The disease has been reported only occurred in sugarcane plantations in Sumatera. Limited information about the disease has slowed down disease diagnosis in the field. The research was aimed to describe symptoms, signs, and spatial distribution of the disease caused by Xylaria. A survey was conducted at a sugarcane plantation in South Sumatera (3° 26’ 16.6” S dan 104°40’ 09.8” E). Typical symptoms of the disease involved drying of all leaves and plant death, dry rot of root and basal stem, and retarded growth of ratoon cane were recorded. Stroma was found on surface of diseased stems and on soil surface of diseased plants, and spatial aggregation of diseased plants was observed in all locations. The distribution of the diseased plant aggregately and the presence of stroma on diseased plants are important characteristics of the disease diagnosis in sugarcane plantations.

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