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KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Published by Universitas Indraprasta PGRI
ISSN : -     EISSN : 25986015     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology
KLOROFIL: Jurnal Ilmu Biologi dan Terapan is a journal that published by Major of biology and applied sciences, Departmen Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sumatera Utara Medan, Indonesia.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 139 Documents
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PENGARUH EKSTRAK DAUN KELOR (Moringa oleifera Lam) TERHADAP KADAR KREATININ, UREUM DAN HISTOLOGI GINJAL TIKUS PUTIH (Rattus norvegicus) YANG DIINDUKSI ETILEN GLIKOL Abdul Karim
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 1 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i1.29576

Abstract

Cases of kidney failure in children have become a public topic in Indonesia in recent years. Acute kidney failure in children is suspected to be related to syrup medication as a causative factor. The syrup suspected of causing kidney failure in children is suspected to be contaminated with ethylene glycol. Ethylene glycol is a compound that can generate free radicals, thus damaging the kidneys. This study aims to examine the effect of Moringa oleifera leaf extract (Moringa oleifera Lam.) on the morphology, histology, and physiology of the kidneys of white rats induced by ethylene glycol. This study used RAL with 20 rats divided into 5 groups, namely the normal control group (feed and drink), the sick control group (0.75% ethylene glycol ad libitum), the Mo 150 treatment group (0.75% ethylene glycol ad libitum and 150 mg/kg BW moringa leaf extract), the Mo 300 treatment group (0.75% ethylene glycol ad libitum and 300 mg/kg BW moringa leaf extract), the Mo 450 treatment group (0.75% ethylene glycol ad libitum and 450 mg/kg BW moringa leaf extract). The stages in this study include phytochemical screening, antioxidant activity testing, total flavonoids, morphological observations, relative organ weight index, scoring of kidney histology damage, examination of kidney urea creatinine levels of white rats. Data analysis used One Way ANOVA and Duncan test. The research results obtained from Moringa leaves did not affect kidney morphology, but had an effect on reducing the relative weight of the organ, scoring of kidney histology damage, creatinine levels, and urea, namely at a dose of 450 mg/kg BW which was effective in reducing the impact of damage due to ethylene glycol administration
Produksi Tanaman Kedelai (Glycine max L. Merrill) Terhadap Pemberian Bradyrhizobium sp dan Kapur di Tanah Mineral Masam Irda Nila Selvia
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 8, No 1 (2024): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN (PROSES)
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v8i1.28062

Abstract

Most Indonesians rely on soybean as their primary source of vegetable protein. Soybean is an agricultural commodity that serves as a basic ingredient in foods such as tofu, tempeh, soy sauce, tauco, oncom, etc. Soybean, as a vegetable protein, is cheaper and more accessible to Indonesians than animal protein. Currently, soybean yield is unable to meet market demand. Therefore, efforts are needed to increase soybean yield by applying Bradyrhizobium sp. and lime to acidic mineral soils to optimize soybean yield on the land. The research used a factorial randomized block design (RBD) with two factors on a 2 × 2 m plot in Cengkeh Turi Village, Binjai. The first factor was the inoculation of Bradyrhizobium sp. inoculants, which included BGR 3, LP 3, and no inoculant. The second factor was liming, which included not supplying and supplying lime at a level of 1.06 kg/plot (pH 4.5 to 5.5). The results showed that the BGR 3 inoculant was able to increase the number of filled pods of soybean plants. The application of lime was able to increase the number of productive branches, the number of filled pods, the dry weight of seeds per plant and the dry weight of 100 soybean seeds. The application of lime was able to increase the dry weight of seeds per plant by 22.17% and increase the dry weight of 100 seeds by 6.72%.
Pengaruh Intensitas Cahaya Matahari Dan Air Terhadap Laju Transpirasi Pada Tumbuhan Zahwa Lopiza
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 1 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i1.30186

Abstract

This study aims to explore how sunlight intensity and water availability influence the transpiration rate in Rhoeo discolor plants. The research method implemented is a basic experiment with two different treatments: one group of plants is exposed to sunlight, while the other group is kept in a dark environment inside a closed cardboard box. Both groups received the same quantity of water, and their weights were measured before and after the treatment to assess weight changes due to transpiration. The factors observed included the initial weight of the plants, their final weight, the weight of water vapor on the covering plastic, and the percentage of water loss. The findings indicate that the plants exposed to sunlight experienced a higher transpiration rate compared to those in the dark. The percentage of water loss in the sunlight treatment reached 10. 8% with a water vapor weight of 3. 4 grams, whereas the dark treatment showed only a 5. 1% water loss with a vapor weight of 0. 7 grams. Based on these results, it can be concluded that sunlight intensity and water availability significantly affect the transpiration rate in Rhoeo discolor plants.
AKTIVITAS BIOLOGIS METABOLIT SEKUNDER PADA TANAMAN UMBI DAN POTENSINYA DALAM APLIKASI INDUSTRI, FARMASI, DAN PANGAN Ahmad Afif Zakariya; Arifa Rizqi Nafisa
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 1 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i1.29267

Abstract

Tuber crops such as cassava (Manihot esculenta), potato (Solanum tuberosum), taro (Colocasia esculenta), cocoyam (Xanthosoma sagittifolium), and sweet potato (Ipomoea batatas) represent important genetic resources with significant potential for utilization across multiple sectors. This study aims to identify metabolites, biological activities, and the utilization of tuber crops through a literature review approach. The results indicate that each plant exhibits diverse metabolite profiles with various biological activities, including phytotoxic, antioxidant, and other functional properties. This metabolic diversity reflects the potential of genetic resources for applications in industrial, pharmaceutical, and food sectors, particularly due to their starch content and phytochemical compounds. Therefore, tuber crops have strong potential to be developed as economically valuable, functional, and sustainable genetic resources.
MENJELAJAH POTENSI MIKROORGANISME DALAM PEMBANGUNAN BERKELANJUTAN (aplikasi dalam pertanian, pangan dan bioteknologi) yulia agustin
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 8, No 1 (2024): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN (PROSES)
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v8i1.15801

Abstract

Mikrobiologi adalah ilmu yang mempelajari mikroorganisme seperti bakteri, virus, fungi, dan protozoa. Seiring perkembangan teknologi dan penelitian dalam bidang mikrobiologi, penggunaan mikroorganisme telah meluas di berbagai bidang, seperti industri makanan, kesehatan, pertanian, dan lingkungan. Tujuan dari penulisan ini adalah untuk menyajikan sebuah analisis tentang potensi mikroorganisme untuk pembangunan berkelanjutan. Melalui studi literatur yang dilakukan, dan bertujuan untuk mengidentifikasi peran mikroba dalam memecahkan masalah dalam kehidupan manusia. Dengan tujuan ini diharapkan dapat memberikan wawasan yang bermanfaat bagi para pembaca yang tertarik dengan peran mikroba dalam kehidupan manusia, serta membantu para pembaca untuk menggetahui macam-macam mikroba dan fungsinya dalam kehidupan sehari-hari.
Biodegradasi Hidrokarbon Tanah Tercemar Limbah Oli Bekas Dengan Biostimulasi Menggunakan Ekoenzim meliana simangunsong
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 2 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i2.30084

Abstract

Soil contamination caused by used motor oil waste is a serious environmental problem because it contains hydrocarbon compounds classified as toxic and difficult to degrade naturally. This study aimed to determine the effect of ecoenzyme addition as a biostimulant on the reduction of Total Petroleum Hydrocarbon (TPH) levels and the growth of indigenous bacterial populations in soil contaminated with used motor oil waste. The study was conducted from August to November 2025 at the Microbiology Laboratory of UIN Sumatera Utara. Contaminated soil samples were taken from an automotive workshop area in the Setia Budi District, Medan Selayang. This experimental study used a Completely Randomized Design (CRD) with treatments consisting of control (K), P1 (0.5 kg soil + 0.5 L ecoenzyme), and P2 (0.6 kg soil + 0.4 L ecoenzyme), with three replications each. Observations were conducted over 35 days at intervals of days 0, 7, 14, 21, 28, and 35. Parameters measured included TPH levels, temperature, pH, moisture content, and bacterial colony counts. Results showed that ecoenzyme addition significantly reduced TPH levels: P1 decreased from 18% to 0.7%, P2 to 2.6%, and the control to 13.4%. Bacterial populations in P1 increased to 3.25 × 10⁶ CFU/mL, P2 reached 2.74 × 10⁶ CFU/mL, while the control only reached 9.81 × 10⁵ CFU/mL. Statistical analysis using One-Way ANOVA showed significant differences among treatments. Treatment P1 was the best condition for reducing TPH levels to below the quality standard (<1%). Ecoenzymes proved effective as biostimulation agents in enhancing microbial activity and accelerating hydrocarbon biodegradation. 
NANOKAPSUL EKSTRAK SIRIH (Piper betle) KOMBINASI BIOSORBEN KITOSAN-ALGINAT TERHADAP DAYA HAMBAT Xanthomonas oryzae pv. oryzae Nur Khurotul A&#039;in; Rofiatun Solekha
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 2 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i2.31754

Abstract

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae is a major constraint in rice cultivation because it can substantially reduce crop yields. Continuous use of synthetic bactericides risks inducing pathogen resistance and environmental pollution, so safer control alternatives are needed. This study aimed to develop and characterize chitosan-alginate-based nanocapsules containing betel leaf (Piper betle) polyphenols and to evaluate their antibacterial activity against Xanthomonas oryzae pv. oryzae. The nanocapsules were fabricated via the ionic gelation method using betel leaf polyphenol extract, chitosan, and alginate. Particle characteristics were analyzed using Scanning Electron Microscopy (SEM) and a Particle Size Analyzer (PSA), while antibacterial activity was assessed using the agar well-diffusion method. SEM results revealed particles with generally good morphology, although some agglomeration was observed. PSA analysis of the best formulation (K3S3) yielded an average particle size of 330.1 nm with a Polydispersity Index (PDI) of 0.169, indicating a relatively uniform size distribution. However, none of the nine formulations produced a measurable inhibition zone against the test bacteria. This outcome is presumed to result from the controlled release of polyphenols from the chitosan-alginate matrix, so that the concentration of active compounds reaching the medium during incubation was insufficient to inhibit bacterial growth. Thus, nanocapsules were successfully formed at the nanoscale with acceptable physical characteristics, but antibacterial activity was not observed under the tested well-diffusion conditions.
Keragaman Serangga Permukaan Tanah Pada Lahan Pertanian Jagung (Zea mays L.):Inventarisasi dengan Perangkap Jebak Purwatiningsih MIPA
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 2 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i2.32257

Abstract

Soil surface insects are an important component of agricultural ecosystems; however, their taxonomic composition in maize agroecosystems can vary depending on habitat conditions and sampling methods. This study aimed to invent the taxonomic composition and evaluate the diversity of soil surface insects in a maize field in Tegal Besar, Jember Regency, East Java, Indonesia. Sampling was conducted in May 2023 using five pitfall traps placed within an area of approximately 300 m². The traps were checked every two days over five sampling periods. Collected specimens were preserved in 70% ethanol and identified based on morphological characteristics. A total of 131 individuals were recorded, representing four orders, six families, and ten subfamilies. Hymenoptera was the order with the highest number of specimens (62 individuals; 47.3%), followed by Orthoptera (44; 33.6%), Coleoptera (24; 18.3%), and Hemiptera (1; 0.8%). The Shannon diversity index, calculated based on the combined abundance at the subfamily level, was H’=1.9876. These results provide baseline information on the taxonomic composition and diversity of soil surface insects captured in the maize agroecosystem at the study site. However, as this study was limited to a single field and utilized only pitfall traps, the findings do not represent the entire insect community within the agroecosystem. Surveys covering more locations and seasons, incorporating complementary sampling methods and measurements of environmental factors, are necessary to gain a more representative understanding of the diversity patterns of soil surface insects in maize agroecosystems.  
PENGARUH EKSTRAK DAUN SEMBUNG RAMBAT (Mikania micrantha Kunth.) TERHADAP HATI PADA TIKUS PUTIH JANTAN (Rattus norvegicus L.) YANG DIBERI PAPARAN ASAP ROKOK Fauzan Al-Amin Harahap; Husnarika Febriani; Nurlian Augustin Ningrum
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 2 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i2.32242

Abstract

Cigarette smoke exposure is known to contain various toxic substances and free radicals that can trigger oxidative stress and cause liver cell damage, characterized by morphological and histological changes as well as elevated SGPT and SGOT enzyme levels. Mikania micrantha Kunth leaves are known to be rich in flavonoids and other secondary metabolites that act as free radical scavengers. This study aimed to determine the effect of Mikania micrantha Kunth leaf ethanol extract on the liver condition of white rats (Rattus norvegicus L.) exposed to cigarette smoke. A Completely Randomized Design (CRD) was employed, involving 20 rats divided into five groups: a normal control group (food and water only); a positive control group (food, water, and cigarette smoke exposure); and the treatment groups P1, P2, and P3 (food, water, cigarette smoke exposure, and Mikania micrantha leaf extract at doses of 100, 200, and 300 mg/kg body weight, respectively). Cigarette smoke exposure was administered daily in the morning for 30 days, while the leaf extract was administered daily in the afternoon from day 8 to day 30. The study procedures included qualitative phytochemical screening, antioxidant activity testing of the extract using the DPPH method, macroscopic examination of liver morphology, histological examination of the liver using the paraffin embedding method and H&E staining, and measurement of SGPT and SGOT levels using the enzymatic kinetic method. Data analysis was performed using One-Way ANOVA followed by Duncan's post-hoc test. The study results indicate that exposure to cigarette smoke can significantly induce morphological and histological changes in the liver—such as alterations in color and organ surface, hydropic degeneration, and hepatocyte necrosis—driven by oxidative stress. This exposure also leads to elevated SGPT and SGOT levels resulting from oxidative stress and damage to hepatocytes. Administration of Mikania micrantha Kunth leaf extract was proven capable of repairing liver damage in male rats (Rattus norvegicus L.); a dosage of 100 mg/kg body weight demonstrated the most optimal effect, bringing the liver close to condition normal.

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