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KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Published by Universitas Indraprasta PGRI
ISSN : -     EISSN : 25986015     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology
KLOROFIL: Jurnal Ilmu Biologi dan Terapan is a journal that published by Major of biology and applied sciences, Departmen Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sumatera Utara Medan, Indonesia.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 109 Documents
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Isolasi dan Identifikasi Bakteri Heterotrofik di Perairan Pantai Paris Tigaras Kabupaten Simalungun Lestari, Sri Winda; Mayasari, Ulfayani; Rasyidah, Rasyidah
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 2 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i2.12894

Abstract

Heterotrophic bacteria are bacteria that have a role as decomposers of waste in the form of carbon compounds such as leaves, wood from plants and animal carcasses which will produce minerals as nutrients, so that waste does not accumulate on the Paris coast. The purpose of this research was to determine the types of bacterial isolates and characteristic of heterotrophic from water Paris beach. Bacterial isolation was carried out using the spread method on nutrient agar medium. The result of isolation was 10 bacteria isolates which were then purified and observation of macroscopic morphology. The result showed that bacterial isolates SP2I1, SP4I4, SP1I7 were heterotrophic. Bacterial isolates SP2I1, SP4I4 was gram–positive with rod-shapes cells, while bacteria isolates SP1I7 was gram-negative (rod-shaped cells). Based the result of biochemical tests, morpjology observations, the bacterial isolates SP2I1, SP4I4 belongs to the genus Bacillus and isolates SP1I7 belongs to the genus Enterobacter.
(PENGEMBANGAN BOOKLET HASIL EKSPLORASI TUMBUHAN BERPOTENSI ANTIHIPERTENSI DI SUKU ANAK DALAM DESA SUNGAI KIJANG) Andriani, Silva
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 2 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i2.17087

Abstract

AbstrackThis study aims to determine the types of antihypertensive plants, plant parts used as medicine, and how to process medicinal plants by the Orang Rimba community in Rawas Ulu District, Sungai Kijang Village, as well as find out the results of the development of the Antihypertensive Plant Exploration Booklet in the Suklu Anak Dalam tribe in Sungai Kijang Village, Rawas Ulu District. The results of the study found 12 species of plants from 10 families. The most widely used plant parts as medicine by the community are leaves (58.85%), while other plant parts used are fruit (30.77%), stems (7.69), rhizomes (7.69%). The most widely used method of processing medicinal plants by the suku anak dalam community in Sungai Kijang Village, Rawas Ulu District, is boiled (57.14%), grated (28.57%) and eaten lasungsung (14.29%). The results of the development of the booklet for the exploration of potentially antihypertensive plants in the suku anak dalam of Sungai Kijang Village, Rawas Ulu District, were carried out by three validators, namely material expert validators by 85%, media expert validators by 83%, linguist validators by 85%, and community readability questionnaires involving small groups of 10 people, showing that the booklet developed was feasible and did not need revision. 
Keanekaragaman Spesies Burung di Gumuk Kerang, Kecamatan Sumbersari, Kabupaten Jember, Jawa Timur Jasmine, Nur Ainna Fairuz
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 2 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i2.16020

Abstract

Burung adalah kelompok satwa liar yang banyak dan mudah dijumpai di berbagai jenis kondisi suatu tipe habitat. Kelompok satwa ini bisa ditemukan dihabitat alami, buatan, ataupun juga bisa hidup disekitar manusia (urban). Dari total keseluruhan jumlah spesies burung, Indonesia memiliki 17% jenis spesies burung. Keragaman jenis burung dapat menjadi indikator lingkungan. Keanekaragaman jenis spesies dapat ditunjukkan dengan adanya jumlah jenis dan jumlah individu yang beragam dalam satu wilayah tertentu. Keanekaragaman jenis dapat digunakan sebagai penilaian awal dalam menilai biodiversitas pada suatu kawasan. Penelitian ini bertujuan untuk menentukan keanekaragaman spesies burung pada wilayah Gumuk Kerang, Kabupaten Jember, Jawa Timur. Penelitian dilakukan pada bulan Mei 2023. Metode yang digunakan yaitu point count (titik hitung) dengan total tiga titik pengamatan. Pengamatan pada setiap titik dengan radius 25 meter dilakukan selama 15 menit dengan mencatat data ekologi yang meliputi nama spesies dan jumlah individu burung yang ditemukan. Analisis data menggunakan Indeks Keanekaragaman Shannon-Wiener (H’).  Berdasarkan hasil penelitian, ditemukan 27 spesies dari 17 famili burung di Gumuk Kerang. Spesies yang memiliki kelimpahan tertinggi yaitu Walet Linchi (Collocalia linchi) dengan rata-rata individu sebanyak 95 ekor. Indeks Keanekaragaman Shannon-Wiener (H’) memiliki nilai 1,970 yang dikategorikan tingkat keanekaragaman sedang. Status konservasi seluruh burung yang ditemukan berdasarkan IUCN adalah Least Concern (LC).
Deteksi Cemaran Bakteri Escherichia coli Pada Ikan Dan Air Sungai Di Perairan Jembatan Cincim Kabupaten Lamongan Purwitasari, Iga Maydah
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 2 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i2.16036

Abstract

Jembatan Cincim merupakan Daerah aliran sungai Bengawan solo yang banyak digunakan untuk berbagai kebutuhan, namun adanya aktivitas di sekitar sungai dapat menyebabkan pencemaran limbah yang sulit terurai dan mudah terakumulasi dalam perairan dan biota air. Penelitian ini bertujuan untuk mengetahui cemaran bakteri Escherichia coli pada ikan dan air sungai di Jembatan Cincim. Penelitian ini merupakan penelitian deskriptif eksploratif dengan pengambilan sampel ikan secara random sampling. Pengambilan sampel dilakukan di perairan Jembatan Cincim. Metode pengujian cemaran bakteri Escherichia coli dilakukan dengan metode Most Probable Number (MPN) dengan seri 3 tabung. Penelitian ini menunjukkan semua sampel ikan dan air sungai positif tercemar bakteri Escherichia coli dengan koloni berwarna hijau metalik. Semua sampel ikan melebihi baku mutu berdasarkan SNI 2729-2013. Nilai bakteri coliform dan coliform fecal pada sampel ikan dan air sungai menunjukkan bahwa sampel telah mengalami kontaminasi. Nilai bakteri tertinggi pada ikan nila  yaitu total coliform >2400 koloni/100 ml dan coliform fecal 1100 koloni/100 ml. Hal ini dapat bersumber dari kondisi perairan sungai yang tercemar limbah sehingga mempengaruhi keberadaan bakteri pathogen dalam ikan sehingga ikan tidak layak untuk dikonsumsi.Kata kunci: Escherichia coli, ikan dan air sungai
Karakterisasi Agronomi 51 Vareitas Unggul Baru Padi (Oryza sativa L.) di Kabupaten Subang Fitrunnisa, Tiara
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 2 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i2.13852

Abstract

Rice (Oryza sativa L.) is the main commodity as a food ingredient in Indonesia considering that rice is a very valuable commodity. Therefore, it is necessary to create new superior varieties that have high yield characteristics, have high nutritional value, smell good, and are resistant to disease. The varieties that are widely used by farmers include the Ciherang and IR 64 varieties. Characterization is carried out to determine the properties or characters possessed by a plant. Based on the characterization carried out, it can be seen the level of dimilarity between varieties by grouping or clustering which have similarities or differences which can then be used to determine the long distance kinship between rice varieties. This study aims to determine the closest and furthest kinship relationships between 51 new superior varieties of rice (Oryza sativa L.). The research was conducted at the Experimental Garden of the Center for Rice Research (BB Padi), in Sukamandijaya Village, Ciasem District, Subang Regency, West Java. The time for conducting the research is September 2021-March 2022. The method used is descriptive, with 51 treatments, namely new superior varieties, repeated 3 times so there are 153 experimental units. In each observational data the agronomic characters were analyzed by clustering using the UPGMA method. Analysis using NTSYS 2.02i and 2.11 software. The results showed that the grouping results of 51 new high yielding varieties of rice (Oryza sativa L.) formed 14 main groups at a similarity value of 0.75 which showed different traits from each other based on agronomic characteristics of plant height, chilbearing ability, panicle discharge, yellowing of leaves, loss and grain fertility. Based on these results, there are 11 closest kinship relationships and have the farthest kinship in inpari 1 and inpago 8 varieties.
Pengaruh Metode Pengolahan Terhadap Total Antosianin dan Uji Aktivitas Antioksidan Pada Jagung Ungu (Zea mays L. var. ceratina Kuleshov) Putriautami, Fernanda Meyra; Nur, Achamd Vandian; Rahmasari, Khusna Santika; Pambudi, Dwi Bagus
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 2 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i2.17237

Abstract

Purple corn is a plant belonging to the Poaceae family which has the Latin name Zea mays L. var. ceratina Kuleshov. Purple corn contains anthocyanins which are beneficial for human health as antioxidant agents. Anthocyanins have low stability at high heat and dissolve easily in water. The purpose of this study was to determine the total anthocyanin levels and antioxidant activity of fresh, steamed, and boiled purple corn. This research was conducted qualitatively using thin-layer chromatography with the mobile phase n-Butanol: ethyl acetate: distilled water (4:1:5) and quantitatively using the differential pH method and the antioxidant activity test using the DPPH method. The results obtained from the TLC test showed anthocyanin standard 0.72; fresh purple corn 0.71; steamed purple corn 0.71, and boiled purple corn 0.71. In the qualitative test of total anthocyanin levels using the differential pH method, the total anthocyanins in fresh purple corn were 235,45 mg/100 g; steamed purple corn of 38,908 mg/100 g, and boiled purple corn of 20,42 mg /100 g. From the antioxidant test with the DPPH method, it was obtained that the best IC50 value in fresh purple corn samples of 33.92 (very strong); steamed purple corn was 124.33 (medium) and boiled purple corn was 126.26 (medium).
Efektivitas Pupuk Organik Cair dengan Bioaktivator B-8 dalam Meningkatkan Pertumbuhan Sorgum (Sorghum bicolor) Nur'aini, Nur'aini
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 1 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i1.24239

Abstract

Sorghum (Sorghum bicolor) is a vital cereal crop known for its resilience in arid and semi-arid regions, making it an essential crop for food security. However, its growth and productivity are significantly affected by soil fertility and nutrient management. This study aimed to evaluate the effectiveness of liquid organic fertilizer (LOF) enriched with the B-8 bioactivator in improving sorghum growth. The experiment utilized a randomized complete block design (RCBD) with five treatments: LOF dilutions of 1:10 (P1), 1:30 (P2), 1:50 (P3), 1:80 (P4), and 1:100 (P5), alongside a control group (P0) without any fertilizer. Each treatment was replicated five times, and plants were monitored for growth parameters such as plant height, stem diameter, and number of leaves, with measurements taken every three days over a 6-week period. Results indicated that the P1 treatment (1:10 dilution) consistently exhibited the highest growth performance across all parameters, reaching a maximum height of 24 cm. The P5 treatment (1:100 dilution) also showed substantial growth, though not as pronounced as P1. In contrast, the control group (P0) showed the lowest growth. These findings suggest that LOF with B-8 bioactivator can significantly enhance sorghum growth, offering a sustainable and eco-friendly alternative to chemical fertilizers for improving crop productivity.
Optimasi Sifat Mekanik dan Morfologi Permukaan Campuran Resiprena dengan Matriks Polimer Polipropilena dan Polistirena melalui Penambahan Stearin Tarigan, Ayu Syufiatun Br; Hartati, Nina; Wirjosentono, Basuki; Arbaiyah, Arbaiyah; Mashitoh, Anjar Siti
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 1 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i1.25687

Abstract

This study aims to investigate the effect of adding resiprene and stearic acid on the mechanical properties and surface morphology of polyolefin blends, specifically polypropylene (PP) and polystyrene (PS). Resiprene was incorporated as an additive to enhance flexibility, while stearic acid was introduced to improve polymer compatibility and blend dispersion. The test results showed that the addition of 20 phr resiprene to polypropylene yielded the highest tensile strength of 9.63 MPa and an elongation of 5.85%, indicating high flexibility and compatibility of the blend. In contrast, the tensile strength of the PS-based blends was generally lower than that of the PP-based counterparts. The addition of 1 phr stearic acid proved effective in reducing the number of pores on the surface of both PP and PS blends. Interestingly, the PS–resiprene–stearic acid blend exhibited a more refined surface morphology compared to the PP–resiprene–stearic acid blend, suggesting higher compatibility of PS within the composite system.
Potensi Bioremediasi Bakteri Indigenous dari Tempat Pembuangan Akhir dalam Mereduksi Metilmerkuri (HgCl₂) Khalidatunnisa, Besse
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 1 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i1.25910

Abstract

Heavy metal contamination, particularly mercury and its derivatives, represents a serious environmental issue with profound impacts on ecosystems and human health. Methylmercury (HgCl₂) is known to be highly toxic, persistent, and capable of bioaccumulating within the food chain. Bioremediation using indigenous bacteria offers an environmentally friendly and sustainable alternative approach. This study aimed to evaluate the diversity and adaptability of soil bacteria in response to mercury (HgCl₂) exposure at different concentrations. Soil samples were incubated with and without HgCl₂, and the number of bacterial strains as well as their growth tolerance were analyzed. The results showed that at day 0, 14 strains were identified in soil without HgCl₂ and 15 strains in soil with HgCl₂. After seven days of incubation, the number of strains slightly increased to 14 and 16 in soil without and with HgCl₂, respectively. Several new strains were detected on day 7, while some were no longer observed in either the control or treated samples. Tolerance assays revealed that certain isolates, such as K7, P1, and P10, were able to grow at mercury concentrations up to 30 ppm, whereas others could only survive at lower concentrations. These variations in growth patterns suggest different adaptive mechanisms, including the potential role of mercury reductase enzymes in detoxification. The findings highlight the ability of soil bacterial communities to survive and adapt to mercury contamination, underscoring their potential role in the bioremediation of heavy metal-polluted environments.

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