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KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Published by Universitas Indraprasta PGRI
ISSN : -     EISSN : 25986015     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology
KLOROFIL: Jurnal Ilmu Biologi dan Terapan is a journal that published by Major of biology and applied sciences, Departmen Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sumatera Utara Medan, Indonesia.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 132 Documents
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Karakteristik Kualitas Cuka Aren (Arenga pinnata Merr) Tradisional Aceh Berdasarkan SNI 01-4371-1996 Masyitah Masyitah
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 2 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i2.28327

Abstract

Palm sap (Arenga pinnata Merr) as the main ingredient in traditional vinegar production. The result of the fermentation process of palm in the form of vinegar.This study aims to determine the quality of traditional palm vinegar (Arenga pinnata) typical of Aceh based on SNI 01-4371-1996. The type of research includes descriptive analysis. Testing the quality of palm vinegar include acetic acid content, Nacl, alcohol content, test total sugar, the amount of dissolved solids, contaminant of metal (Pb, Cu, Zn), arsenic contamination, microbial contamination and pH. The results showed that the standart that meet on vinegar are the liquid form and acid odor, residue of alcohol 3,54%, contaminant of metal Pb and Cu 0,20 mg/kg and 0,31 mg/kg, microbial contamination 1×10-1, and not detection of arsenic contaminant. pH test 3,27. Standart that not meet are the total acetic acid content 0,18%, Nacl 0,06, the amount of dissolved solids 5,2 °Brix and contaminant of metals Zinc (Zn) 2,76 mg/kg.
EFEK PEMBERIAN EKSTRAK ETANOL BUAH GANDARIA (Bouea macrophylla Griffith.) TERHADAP GAMBARAN MORFOHISTOLOGI LIMPA TIKUS PUTIH (Rattus norvegicus L.) YANG DIINDUKSI KARBON TETRAKLORIDA (CCL4) Ahmad Baihaqi Ananda El Rawy; Husnarika Febriani; Nurlian Augustin Ningrum
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 2 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i2.28555

Abstract

Carbon tetrachloride (CCl4) is a volatile liquid that can cause hepatotoxicity in metabolism, because it produces Reactive Oxygen Species (ROS) which can trigger inflammation and cell dysfunction. Gandaria fruit (Bouea macrophylla Griffith) is known to contain a number of antioxidant compounds that function as free radical scavengers. This study aims to determine the effect of administering ethanol extract of gandaria fruit (Bouea macrophylla Griffith.) on the morphology and histology of the spleen of white rats (Rattus norvegicus L.) induced by carbon tetrachloride (CCl4). This study used RAL using 25 rats divided into 5 groups, Negative controls was only given food and drink, Positive controls was given CCl4 1mL/kgBW Treatment P1, P2, P3, was given CCl4 1 mL/kgBW + gandaria fruit extract with doses of P1 (100 mg/kgBW), P2 (150 mg/kgBW), P3 (200 mg/kgBW). CCl4 administration was carried out every 3 days for 30 days and gandaria fruit extract was administered daily starting from day 8 to day 30.The stages in this study include qualitative phytochemical screening tests, antioxidant activity tests using the DPPH method, macroscopic analysis of the morphological condition of the spleen organ and histological examination of the spleen organ using the paraffination and HE staining methods. Data analysis used the one-way ANOVA test and continued with Duncan's advanced test. The results showed that administration of gandaria fruit ethanol extract did not affect the morphology of the spleen organ of white rats induced by carbon tetrachloride (CCl4) including the edge shape, color, surface, texture, weight, length and width. However, induction of carbon tetrachloride at a dose of 1 mL/kgBW can damage the histological condition of the spleen tissue including bleeding and necrosis and widening the diameter of the white pulp, the diameter of the germinal center and the marginal zone. Administration of gandaria fruit ethanol extract in the P3 treatment at a dose of 200 mg/kgBW was the most effective dose in repairing spleen organ tissue.
Diversitas Protista Pada Perairan Air Payau Riska Agnesia
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 1 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i1.27863

Abstract

This review aims to determine the diversity of protists in brackish waters, the methodologies of several researchers, and compare the results obtained from various researchers. Some examples of locations and ecosystem types where protists were discovered include mangrove forests, coastal areas, swamps, aquaculture ponds, and river estuaries. Most journals conducted field research for identification and diversity analysis. However, some used DNA metabarcoding techniques for taxonomic and functional analysis, as well as comprehensive literature reviews. The results showed that the majority of protists discovered were animal-like protists (protozoa) and plant-like protists (algae), as well as plankton communities such as phytoplankton and zooplankton. The research results can be used a learning resource, demonstrating and educational aspect.
Pengaruh Penambahan Sari Lemon Terhadap Fermentasi Acar (Pickled Cucumber) Timun Berbasis Probiotik Desi Nurlita
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 1 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i1.27903

Abstract

The purpose of this study is to illustrate how adding lemon juice affects the fermentation process of cucumber pickles made with probiotics. A mixture of lemon juice, water, and salt was used to carry out the fermentation process. At room temperature, fermentation was carried out for seven days. Changes in pH, solution color, aroma, and the physical state of the cucumbers during fermentation were among the criteria that were noted. According to the findings, the pH progressively dropped from 5.6 on the first day to 4.2 on the seventh, suggesting that lactic acid bacteria activity was creating an acidic environment. The proper fermentation process is indicated by the solution's hue changing to a lighter shade and the emergence of a distinctive sour fermentation fragrance.
PENGARUH EKSTRAK DAUN KETAPANG (Terminalia catappa) TERHADAP HISTOLOGI HATI IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI BAKTERI Aeromonas hydrophila Khusnul Adinda Arisanti; Efrida Pima Sari Tambunan; Nurlian Augustin Ningrum
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 2 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i2.29505

Abstract

Aeromonas hydrophila is a rod-shaped bacteria (bacillus), root, motile, with a diameter of 0.3-1 µm and a length of 1-3.5 µm, without a spore phase, generally does not have a capsule and grows optimally at a temperature of 28°C but can grow at extreme temperatures (4°C-37°C). Aeromonas hydrophila causes a disease in fish known as Motile Aeromonas Septicemia (MAS), Aeromonas hydrophila infection can cause liver tissue damage such as fatty degeneration, hemorrhage, necrosis, dilation of the central vein, dilation of hepatocytes, dilation of sinusoids, and changes in the size of the cell nucleus. Ketapang leaves (Terminalia catappa) are known to be rich in active compounds of tannins and flavonoids which have antibacterial properties. This study aims to determine the potential content and efficacy of ketapang leaf extract (Terminalia catappa) in improving the morphology and histology of the liver of tilapia (Oreochromis niloticus) caused by Aeromonas hydrophila bacterial infection. This study used RAL using 30 tilapia (Oreochromis niloticus) which were divided into 6 treatment groups, Normal Control (KN): feed without Aeromonas hydrophila bacterial infection. Negative Control (K-): Aeromonas hydrophila bacterial infection. Positive Control (K+): (fish infected with bacteria + Red Bluedox). Treatment 1: (fish infected with bacteria + 30 ppm ketapang leaf extract). Treatment 2: (fish infected with bacteria + 50 ppm ketapang leaf extract). Treatment 3: (fish infected with bacteria + 70 ppm ketapang leaf extract). The stages in this study included phytochemical screening tests, antioxidant activity tests and morphological and histological examinations of the liver of tilapia (Oreochromis niloticus). Data analysis used one-way ANOVA test and continued with Duncan's advanced test. The results of the study showed that soaking in ketapang leaf extract (Terminalia catappa) had no significant effect on the liver morphology of tilapia fish (Oreochromis niloticus) infected with Aeromonas hydrophila bacteria, while soaking in ketapang leaf extract at a dose of 70 ppm could repair histological damage to the liver of tilapia fish infected with Aeromonas hydrophila, namely with a hemorrhagic damage score (7.75), a fatty degeneration damage score (9.50), and a necrosis damage score (11.00). Meanwhile, histomorphometric observations with a dose of 70 ppm obtained good results for improving the diameter and area of each cell observed, namely the diameter of the central vein (19.54µm), the area of the central vein (306.94µm2), the diameter of hepatocytes (9.03µm), the area of hepatocytes (64.57µm2), the diameter of the hepatocyte nucleus (3.06µm), the area of the hepatocyte nucleus (64.57µm2), the diameter of the sinusoid (7.54µm), and the area of the sinusoid (45.35µm2).
Mekanisme Pengangkutan Air pada Tanaman Allamanda chatartica Nazilla Khoridatul Hidayah; Gatra Ervi Jayanti
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 2 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i2.25471

Abstract

Water transport in the plant body depends on the efficiency of xylem and phloem networks. This study aims to observe the role of transport tissues, especially xylem, in the process of water transportation in Allamanda cathartica plants. The research method was conducted with three different treatments on the plant stem: xylem and phloem open (control), xylem closed phloem open, and phloem closed xylem open. Each treatment was observed for 10 days to record the decrease in water level. Results showed that the treatment with open xylem absorbed the most water compared to the other treatments, while phloem closure had no significant impact on water absorption. This finding confirms that xylem is the main pathway in the transportation of water in plants, while phloem is more involved in the distribution of photosynthetic products.Keywords: Allamanda cathartica; phloem; transport tissue; xylem.
KOMPARASI STRUKTUR DAN PERKEMBANGAN UBUR-UBUR BINTIK (Mitiagas papua) DAN UBUR-UBUR BULAN (Aurelia aurita) SEBAGAI RESPONS ADAPTIF TERHADAP PERBEDAAN LINGKUNGAN Yeni Ismawati
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 1 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i1.30245

Abstract

Mastiagas papua and Aurelia aurita are two scyphozoan jellyfish species that differ in body structure, development, and environmental adaptation. This study aims to compare the characteristics of both species based on a literature review. The review was conducted through the collection of relevant scientific articles and analyzed descriptively-comparatively. The results showed that Mastiagas papua has a thicker and denser body, a golden coloration, shorter tentacles that are less active in capturing prey than those of Aurelia aurita, and a symbiotic relationship with zooxanthellae that makes it highly dependent on light and stable environmental conditions. In contrast, Aurelia aurita has a thinner and more transparent body, longer tentacles, and a more complex and flexible life cycle, allowing it to adapt to a wider range of aquatic environments. These differences indicate that the body structure and development of the two species are closely related to their respective habitat adaptation strategies. 
Ekstraksi dan Identifikasi Senyawa Fitokimia Bunga Telang (Clitoria ternatea) Indriyani Indriyani
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 1 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i1.29954

Abstract

Butterfly pea flower (Clitoria ternatea) is a herbal plant known for its health benefits due to the presence of various bioactive compounds. This study aimed to identify the phytochemical compounds contained in butterfly pea flowers through an extraction method using ethanol as the solvent. The flower samples were dried and homogenized using a homogenizer until a fine powder was obtained. The powder was then macerated with 96% ethanol for 2 × 24 hours to obtain a concentrated extract. The resulting extract was analyzed through qualitative phytochemical screening to identify the presence of qualitative phytochemical screening to identify the presence of secondary metabolites, including alkaloids, flavonoids, saponins, tannins, phenols, steroids, and terpenoids. The results showed that butterfly pea flower extract positively contained flavonoids, saponins, tannins, phenols, steroids, and terpenoids. The presence of potential as a natural material with biological activity, particularly as a source of antioxidants. This study is expected to provide supporting data for the development and utilization of butterfly pea flower in the health sector and natural-based products. 
Kandungan Klorofil dan Kerapatan Stomata Spesies Invasif Chromolaena odorata pada Kondisi Naungan yang Berbeda Devi Eka Lestari; Nida Lidya Susanti; Rohimatul Anwar
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 1 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i1.30307

Abstract

Kirinyuh plant (Chromolaena odorata) is one of the most aggressive invasive plant species, with a strong ability to colonize diverse habitats under varying environmental conditions. Light availability is a key environmental factor influencing the establishment and spread of invasive species, as it affects leaf physiological and anatomical traits related to photosynthesis and resource use. This study aimed to evaluate the ecophysiological responses of C. odorata under different shading conditions based on chlorophyll content and stomatal characteristics. Leaf samples were collected from plants growing in open and shaded habitats. The parameters observed included chlorophyll a, chlorophyll b, total chlorophyll content, and stomatal density. Chlorophyll was analyzed using a 95% ethanol extraction method followed by UV–Vis spectrophotometry. Stomatal density was determined through leaf sectioning and safranin staining. The results showed that shaded plants had higher chlorophyll a, chlorophyll b, and total chlorophyll contents compared to those in open habitats, while stomatal density was lower under shaded conditions. These findings indicate that C. odorata can adjust its physiological and anatomical traits in response to light variation, supporting its ecological adaptability.
KEANEKARAGAMAN MARGA JAMUR PATOGEN PADA BIJI KACANG TANAH (Arachis hypogaea L.) DI PASAR TRADISIONAL KECAMATAN KEDATON, BANDAR LAMPUNG Annida Amalia Fatin; Yulianty Yulianty; Lili Chrisnawati; Sri Wahyuningsih
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 10, No 1 (2026): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v10i1.28786

Abstract

This study aims to identify the diversity and dominant genera of pathogenic fungi contaminating peanut (Arachis hypogaea L.) seeds sold in traditional markets in Kedaton District, Bandar Lampung. Peanut samples were collected from seven traditional markets using purposive sampling and analyzed through macroscopic and microscopic observations. Fungal isolation was performed using Potato Dextrose Agar (PDA), followed by identification using slide culture techniques and standard fungal identification keys. The findings revealed three fungal genera, namely Aspergillus sp., Rhizopus sp., and Penicillium sp. Aspergillus sp. was found to be the most dominant, appearing in 16 out of 21 samples. The dominance of Aspergillus sp. is associated with its strong ability to grow in warm, humid, and open storage environments typical of traditional markets. The presence of these fungi is of concern, as some species are known to produce harmful mycotoxins that may threaten food safety. The study concludes that pathogenic fungal contamination in peanuts is common in traditional markets, highlighting the need for improved postharvest handling and storage practices.

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