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Current Research on Biosciences and Biotechnology
Published by Institut Teknologi Bandung
ISSN : -     EISSN : 26861623     DOI : https://doi.org/10.5614/crbb
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology
The Current Research on Biosciences and Biotechnology (CRBB) encourages researchers to publish works related to the use of the biosciences and biotechnology in understanding the world around us. From Health and Medicine to Advanced Materials, these state-of-the-art research offer novel insights in a multidisciplinary environment. We encourage scientists to submit papers focusing on the following broad topics: Health and Medicine Pharmaceutical Sciences Pharmacology and Toxicology Food Microbiology and Biotechnology Biotechnology Agriculture Bio-Energy Marine Sciences Advanced Materials Environmental Sciences
Arjuna Subject : Umum - Umum
Articles 5 Documents
 1 
Search results for , issue "Vol. 5 No. 1 (2023)" : 5 Documents clear
Comparison of the effect of Sumbawa honey and sucrose as extracellular cryoprotectants on viability, morphology, and phenotype stability of CD34+ hematopoietic stem cells from umbilical cord blood Samuel Febrian Wijaya; Retno Lestari; Inna Rahmawati; Imelda Rosalyn Sianipar; Robby Nuraditya; Iqbal Fasha; Gita Pratama; Radiana Dhewayani Antarianto
Current Research on Bioscences and Biotechnology Vol. 5 No. 1 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.5.1/CBWGKPBX

Abstract

Cryopreservation is a common practice for the long-term storage and maintenance of the quality of Hematopoietic Stem Cells (HSC) from Umbilical Cord Blood (UCB). Cryomedium containing 10% DMSO is the standard intracellular cryoprotectant agent (CPA) in HSC cryopreservation. However, DMSO is toxic to both cells and patients during transplantation. Therefore, the concentration of DMSO needs to be reduced by adding extracellular CPAs, such as sucrose or Sumbawa honey. The objective of this study was to compare the ability of Sumbawa honey and sucrose as extracellular CPAs to protect HSC CD34+ during cryopreservation. This in vitro study was designed using cryomedium consisting of 10% DMSO as a control, 5% DMSO + 5% Sumbawa honey, and 5% DMSO + 5% sucrose. The results showed that the cryoprotectant containing 5% DMSO + 5% Sumbawa honey had a positive effect and a significant difference (p<0.05) compared with 5% DMSO + 5% sucrose on the viability and morphology of HSC. However, the mean reduction in phenotype stability as indicated by the decrease in percentage CD34+ in the 10% DMSO (6.90 ± 8.60), 5% DMSO + 5% sucrose (10.60 ± 9.20), and 5% DMSO + 5% Sumbawa honey (8.60 ± 11.50) showed no significant difference (p>0.05). In conclusion, the combination of DMSO and Sumbawa honey was able to maintain the viability, morphology, and phenotype stability of HSC. Therefore, honey can be used as an alternative cryoprotectant for the cryopreservation of hematopoietic stem cells.
Biohydrometallurgy: paving the way for a greener future of mineral processing in Indonesia - A mini review Siti Khodijah Chaerun; Ronny Winarko; Frideni Yushandiana
Current Research on Bioscences and Biotechnology Vol. 5 No. 1 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.5.1/8KIZ3AOE

Abstract

Biohydrometallurgy, a technology that employs microorganisms for metal extraction, has existed since the 1960s. As environmental regulations tighten and the quality and complexity of available ores for processing decline, this technology offers an alternative for mineral processing. Several countries, including South Africa, Russia, Chile, Australia, the United States, China, Burma, New Zealand, Peru, Uzbekistan, and Ghana, have used this method commercially in copper processing plants and gold and silver processing plants. In Indonesia, this method has not been developed or applied industrially. Given the challenges of limited capital and low-grade ore processing in the future, proposing biohydrometallurgical processing in Indonesia is worthwhile. Globally, biohydrometallurgy has become a significant area of research focus. In Indonesia, however, the investigation of biohydrometallurgy is primarily conducted at the Bandung Institute of Technology (ITB). This specific line of investigation was initiated in 2009, with an emphasis on extracting nickel (Ni) from laterite ores. Additional investigations have been undertaken to explore the extraction of metals including copper (Cu) and gold (Au). This review paper also summarizes ongoing laboratory-scale studies encompassing the extraction of lead (Pb), zinc (Zn), silicon (Si), magnesium (Mg), silver (Ag), rare earth elements (REEs), lithium (Li), strontium (Sr), phosphorus (P), potassium (K), calcium (Ca) and the application of phytomining technology and coal biomining. The research outcomes to date present a promising and potentially scalable perspective that could be advanced to pilot plant implementation and industrial application within Indonesia.
Sun protection factor and tyrosinase inhibitory activity of several plant secondary metabolites Raihana Mufliha Fatharani; Ula Aulia Fitrian; Sumail Sidik Ode Ishak; Amirah Adlia
Current Research on Bioscences and Biotechnology Vol. 5 No. 1 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.5.1/VCLQ3ADV

Abstract

Severe exposure to ultraviolet (UV) rays leads to skin damage, including hyperpigmentation, freckles, melanoma, age spots, and melasma, all of which are related to the skin pigment enzyme, tyrosinase. Prevention can be achieved by avoiding harsh UV rays and inhibiting tyrosinase catalytic activity. Many compounds have been developed for the treatment of such conditions; however, most come with unwanted side effects. The purpose of this study was to determine the sun protection factor (SPF) value and tyrosinase enzyme inhibitory activity of plant secondary metabolites with high antioxidant activity, namely rutin, catechin, niazirin, piperine, quercetin, and quercitrin, as potential alternatives. Both tests were carried out using UV-Vis spectrophotometry. SPF determination was performed by observing the absorbances across a wavelength range of 290 to 320 nm. The determination of anti-tyrosinase activity was conducted by measuring dopachrome at 490 nm after enzymatic reactions and calculating the IC50 value. In the SPF assay, benzophenone-3 was used as the standard, and piperine, rutin, quercetin, and quercitrin exhibited high protective abilities with SPF values above 30 at 500 µg/mL. In the tyrosinase inhibition assay, kojic acid as the standard showed a strong potential for inhibition with an IC50 of 33.65 µg/mL, while quercetin, rutin, and piperine exhibited weaker inhibitory potential with IC50 values of 178.44, 271.73, and 347.62 µg/mL, respectively. On the other hand, quercitrin and niazirin showed little to no tyrosinase inhibition activity. However, catechin demonstrated more catalytic activity towards the enzyme. In conclusion, quercetin, rutin, and piperine have the potential to be developed as active ingredients to protect the skin from UV-induced damage due to their satisfactory SPF values and tyrosinase inhibition activity.
Assessment of nutritional value and investigation of biological activities of Hylocereus costariscensis Md. Mostafa Hossain; Mohammad Shoeb
Current Research on Bioscences and Biotechnology Vol. 5 No. 1 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.5.1/TT1LHNS7

Abstract

Hylocereus costariscensis (Dragon fruit), an edible cactus species of the Cactaceae family, is rich in essential nutrients like antioxidants, dietary fibers, minerals, and vitamins. The aim of the study was to analyze the proximate composition and investigate the biological activities of the edible parts (flesh and seed) of H. costariscensis cultivated in Bangladesh. Air-dried fruits were successively extracted by n-hexane, dichloromethane, and methanol. The moisture and ash contents of the fruits were found to be 85.95 ± 0.53% and 0.99 ± 0.02%, respectively. UV and FT-IR spectral analysis showed the presence of different functional groups, which might be due to the presence of fatty acids, alkaloids, terpenoids, and phenolic compounds. The total carbohydrate content was 10.52g/100g as determined by the modified Molisch method. Fatty acid analysis revealed the presence of octanoic acid (5%), palmitic acid (16%), octadecanoic acid (41%), cis-9-oleic acid (29%), and linoleic acid (6%). The total phenolic content of different extracts was determined by the Folin–Ciocalteau method, and the value was found to be higher in the methanol extract (151.05 ± 0.34 mg GAE/g) than in the other two extracts. Total flavonoid content and total antioxidant content were determined by the aluminum chloride method and the phosphomolybdate assay, respectively. The methanol extract exhibited the highest activities (91.54 ± 0.22 mg QE/g and 40.08 ± 0.21 mg AAE/g, respectively) among all extracts in both cases.
Chemical and biological activity studies of Averrhoa carambola L. Sharmin Shahjahan; Mohammad Shoeb; Md. Mazharul Islam; Md. Iqbal Rouf Mamun; Md. Nazrul Islam
Current Research on Bioscences and Biotechnology Vol. 5 No. 1 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.5.1/0UFAMUR3

Abstract

Averrhoa carambola L., belonging to the Oxalidaceae family is one of the popular fruits in Bangladesh. The objective of the study is to isolate and purify secondary metabolites from A. carambola leaves and investigate phytochemical properties, fatty acid, antioxidant, antimicrobial activity, and cytotoxicity in both fruits and leaves. Phytochemical screening revealed the presence of lignins, quinones, saponins, flavonoids, phenol, carbohydrates, steroids, and terpenoids. Repeated column chromatography of the dichloromethane (DCM) extract of leaves yielded three compounds: β-sitosterol, 6,9-dieneoctadeca-1-ol, and palmitic acid, characterized using spectroscopic techniques. The n-hexane extract of the fruit showed the presence of palmitoleic acid (20.94%) and octadecanoic acid (48.48%), while palmitoleic acid (6.47%), octadecanoic acid (23.23%), and behenic acid (41.70%) predominated in the leaves. For DPPH free radical scavenging activity, the IC50 value of methanol (MeOH) extracts was found to be 16.71 and 27.30 µg/mL in fruits and leaves, respectively, which is lower than that of n-hexane (IC50 value 282.46 and 78.65 µg/mL in fruits and leaves, respectively) and DCM (IC50 value 32.98 and 39.38 µg/mL in fruits and leaves, respectively) extracts. None of the extracts showed significant antibacterial or antifungal activities. Cytotoxicity assay was performed on HeLa and Vero cell lines, and only the DCM extract of fruits exhibited cytotoxicity on the Vero cell line.

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