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All Journal Universa Medicina Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Ilmu Faal Olahraga Indonesia Current Research on Biosciences and Biotechnology eJournal Kedokteran Indonesia Jurnal Kedokteran Meditek
Imelda Rosalyn Sianipar
Department Of Medical Physiology And Biophysics, Faculty Of Medicine Universitas Indonesia, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Public Health

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LOW VITAMIN B12 DIET INCREASES LIVER HOMOCYSTEINE LEVELS AND LEADS TO LIVER STEATOSIS IN RATS Sianipar, Imelda Rosalyn; Ujianti, Irena; Yolanda, Sophie; Jusuf, Ahmad Aulia; Kartinah, Neng Tine; Amani, Patwa; Murti, Krishna Aditya; Soeria Santoso, Dewi Irawati
Universa Medicina Vol 38, No 3 (2019)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (821.825 KB) | DOI: 10.18051/UnivMed.2019.v38.194-201

Abstract

Background Nonalcoholic fatty liver disease (NAFLD) is one of the most widespread chronic liver diseases, caused by the development of insulin resistance. One of the mechanisms involved is a disturbance in insulin signaling by certain toxic substances that interact with one of the proteins responsible for the insulin signaling pathway. Increased homocysteine level, upon disruption of the methionine pathway, is associated with insulin resistance. The aim of this study was to evaluate the effect of hyperhomocysteinemia and insulin resistance (HOMA-IR level) induced by dietary vitamin B12 restriction on liver steatosis. Methods A study of laboratory experimental design was conducted involving 18 male Sprague Dawley rats (age 36-40 weeks, BW 300-350 g), that were randomly divided into 3 groups: control, 8-week treatment, and 16-week treatment. Standard AIN-93 diet was administered to the control group, whereas rats in the treatment groups were fed vitamin B12 deficiency-AIN-93M. At the end of treatment, liver homocysteine levels were determined by ELISA, HOMA-IR values were calculated, and steatosis degree of the liver was determined histologically. Statistical analysis was performed using independent t-test. Results A significant increase in liver homocysteine levels was found between the control and both the 8- and 16-week treatment groups (p<0.001). HOMA-IR levels were significantly higher in both treatment groups compared to controls (p<0.001). The area of liver steatosis in both treatment groups was significantly larger than that of the control group (p<0.001). Conclusion Increased homocysteine levels due to dietary vitamin B12 deficiency induces liver steatosis due to insulin resistance in rats.
PERAN LATIHAN FISIK DALAM PENANGANAN OBESITAS: AKSI IRISIN PADA PROSES PENCOKELATAN Dewi Irawati Soeria Santoso; Imelda Rosalyn Sianipar; Neng Tine Kartinah
JURNAL ILMU FAAL OLAHRAGA INDONESIA Vol 3, No 1 (2020)
Publisher : PAIFORI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.616 KB) | DOI: 10.51671/jifo.v3i1.86

Abstract

In recent years, the prevalence of obesity continues to increase, leading to a public health problem. Therefore, the obesity problem needs serious attention and treatment approaches. Exercise is one of the treatment approach to combat obesity because exercise plays a role in beiging/browning process. Beiging is a differentiation process from white adipocyte to beige adipocyte, which has similar characteristics to brown adipocyte and is marked with an increase of UCP-1 expression. Irisin plays a role in increasing UCP-1 expression by activating p38 mitogen-activated protein kinase (MAPK) and extracellular-signal regulated kinase (ERK) signaling. Muscle contraction during exercise can activate PGC-1α, which leads to the synthesis of irisin. Exercise may increase irisin levels in skeletal muscle and consequently, play as a mediator of beiging process in adipose tissue.
Comparison of the effect of Sumbawa honey and sucrose as extracellular cryoprotectants on viability, morphology, and phenotype stability of CD34+ hematopoietic stem cells from umbilical cord blood Samuel Febrian Wijaya; Retno Lestari; Inna Rahmawati; Imelda Rosalyn Sianipar; Robby Nuraditya; Iqbal Fasha; Gita Pratama; Radiana Dhewayani Antarianto
Current Research on Bioscences and Biotechnology Vol. 5 No. 1 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.5.1/CBWGKPBX

Abstract

Cryopreservation is a common practice for the long-term storage and maintenance of the quality of Hematopoietic Stem Cells (HSC) from Umbilical Cord Blood (UCB). Cryomedium containing 10% DMSO is the standard intracellular cryoprotectant agent (CPA) in HSC cryopreservation. However, DMSO is toxic to both cells and patients during transplantation. Therefore, the concentration of DMSO needs to be reduced by adding extracellular CPAs, such as sucrose or Sumbawa honey. The objective of this study was to compare the ability of Sumbawa honey and sucrose as extracellular CPAs to protect HSC CD34+ during cryopreservation. This in vitro study was designed using cryomedium consisting of 10% DMSO as a control, 5% DMSO + 5% Sumbawa honey, and 5% DMSO + 5% sucrose. The results showed that the cryoprotectant containing 5% DMSO + 5% Sumbawa honey had a positive effect and a significant difference (p<0.05) compared with 5% DMSO + 5% sucrose on the viability and morphology of HSC. However, the mean reduction in phenotype stability as indicated by the decrease in percentage CD34+ in the 10% DMSO (6.90 ± 8.60), 5% DMSO + 5% sucrose (10.60 ± 9.20), and 5% DMSO + 5% Sumbawa honey (8.60 ± 11.50) showed no significant difference (p>0.05). In conclusion, the combination of DMSO and Sumbawa honey was able to maintain the viability, morphology, and phenotype stability of HSC. Therefore, honey can be used as an alternative cryoprotectant for the cryopreservation of hematopoietic stem cells.
Neuroprotective effect of South Sulawesi propolis on neurogenesis in primary cultures of rat cerebral cortical neurons Nurhadi Ibrahim; Trinovita Andraini; Amirah Yusnidar; Putu Indah Paramita Adi Putri; Imelda Rosalyn Sianipar
Universa Medicina Vol. 42 No. 2 (2023)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2023.v42.137-149

Abstract

BackgroundNeurodegenerative diseases have a significant risk factor, namely aging, which is associated with increased neuronal dysfunction and death. Propolis has been widely used as medicine due to its various benefits. This research study investigated the effect of propolis from the stingless bee (Tetragonula sapiens) from South Sulawesi, Indonesia, on neurogenesis in primary cultures of embryonic cerebral cortex of Wistar rats at 17-18 days of gestation. Methods This research was an experimental study involving 4 female pregnant Wistar rats, which were terminated and the cerebral cortex of the embryos collected and grown as primary cultures. The cultures were divided into 3 groups, i.e. control, vehicle, and propolis extract group. The research began with 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) to obtain the optimal dose among propolis doses of 0.5 μg/mL, 1 μg/mL, 5 μg/mL, 10 μg/mL, 25 μg/mL, and 100 μg/mL. The study was continued by using the best dose in immunostaining examination using microtubule-associated protein 2 (MAP2) primary antibody and qRT-PCR examination of brain-derived neurotrophic factor (BDNF) mRNA expression. One Way ANOVA and Kruskal-Wallis test were used to analyse the data. ResultsThe results showed that the propolis doses of 0.5 μg/mL and 1 μg/mL significantly increase cell viability compared to the other doses (p=0.011) and stimulate dendritic growth. The propolis dose group of 1 μg/mL induces a significantly higher expression of BDNF mRNA than the control group (p=0.031). ConclusionOur findings indicate that stingless bee propolis has neuroprotective effects against BDNF mRNA in rats. It is shown that propolis can be a candidate inhibitor in neurodegenerative diseases.
Ekspresi Protein Foxo1 dan Gen Glukosa 6 Fosfatase pada Tikus dengan Diet Restriksi Vitamin B12 Imelda Rosalyn Sianipar; Trinovita Andraini; Dewi Irawati Soeria Santoso; Irena Ujianti; Marcel Antoni
Jurnal Kedokteran Meditek Vol 28 No 2 (2022): MEI-AGUSTUS
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v28i2.2351

Abstract

Pada penelitian awal didapatkan diet restriksi vitamin B12 menyebabkan hiperhomosisteinemia dan resistensi insulin, yang ditandai oleh hiperglikemia dan meningkatnya nilai Homeostatic Model Assessment for Insulin Resistance (HOMA-IR). Dengan menggunakan sampel jaringan biologik tersimpan, hati tikus Spraque-Dawley dari penelitian tersebut, penelitian lanjutan ini bertujuan mengetahui penyebab hiperglikemia, dalam hubungannya dengan proses glukoneogenesis, dengan melihat ekspresi forkhead box protein-O1 (FoxO1) dan gen glukosa 6 fosfatase (G6Pc). Adapun sampel terdiri dari 4 kelompok: kelompok kontrol dan tiga kelompok dengan diet restriksi vitamin B12 masing-masing selama 4, 8, dan 12 minggu. Ekspresi FoxO1 diperiksa dengan metode kuantitatif Western-Blot, sedangkan gen G6Pc diperiksa dengan metode real-time Polymerase Chain Reaction (rt-PCR). Hasil yang diperoleh, tidak terdapat perbedaan bermakna ekspresi FoxO1 (P > 0,05) dan gen G6Pc (P > 0,05) antara kelompok tikus kontrol dan kelompok diet restriksi vitamin B12. Hal ini menunjukkan, hiperglikemia pada diet restriksi vitamin B12 tidak terkait dengan glukoneogenesis. Pada kondisi resistensi insulin, insulin masih dapat meneruskan efek metaboliknya melalui jalur lain, seperti melalui reseptor yang memiliki kemiripan struktur dan fungsi dengan reseptor insu   lin. Penyebab-penyebab lain terjadinya hiperglikemia seperti gangguan utilisasi glukosa oleh sel dan gangguan proses glikogenesis perlu diteliti lebih lanjut.
Peran HIIT dan MICT terhadap Kadar Angiotensin II, Ekspresi Fibronektin, dan Gambaran Histologis Aorta pada Model Tikus Hipertensi Induksi NOS Inhibitor Naibaho, Fitri Boru; Santoso, Dewi Irawati Soeria; Paramita, Nurul; Jalma, Monica Dwi; Goenarjo, Roman Ardian; Sianipar, Imelda Rosalyn
eJournal Kedokteran Indonesia Vol. 12 No. 2 (2024): Vol. 12 No. 2 - Agustus 2024
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Salah satu mekanisme adaptif terhadap hipertensi adalah perubahan struktur pembuluh darah, sehingga perubahan tekanan darah berhubungan dengan perubahan struktur aorta. High-Intensity Interval Training (HIIT) dan Moderate-Intensity Continuous Training (MICT) berperan menurunkan hipertensi dan memperbaiki kerusakan pembuluh darah. Tujuan penelitian ini ialah untuk mengetahui perbandingan HIIT dan MICT terhadap kadar angiotensin dan fibronektin tikus hipertensi yang diinduksi N(gamma)-nitro-L-arginine methyl ester (L-NAME) dengan desain penelitian eksperimental. Penelitian ini dilakukan di empat Laboratorium di lingkungan Fakultas Kedokteran Universitas Indonesia pada bulan Maret 2023–Januari 2024. Penelitian ini menggunakan 24 ekor tikus putih jantan galur Wistar (Rattus Novergicus) usia 10–13  minggu dengan berat 220–300 g di awal penelitian. Tikus dibagi menjadi empat kelompok yaitu: tikus kontrol, tikus yang diberi L-NAME secara oral selama 5 minggu, tikus L-NAME yang diberi perlakuan HIIT dan kelompok tikus L-NAME yang diberi perlakuan MICT. Penelitian awal menunjukkan tikus HIIT mengalami penurunan berat badan yang bermakna  dibandingkan kontrol. Pada tikus yang diinduksi L-Name, tekanan darah  meningkat dibandingkan kelompok kontrol. Setelah perlakuan 5 minggu,tekanan darah pada kelompok L-NAME, HIIT dan MICT menurun tidak bermakna  dibandingkan dengan kontrol.  Kadar angiotensin II pada kelompok HIIT menunjukkan penurunan tekanan darah secara bermakna  dibandingkan MICT dan HIIT secara bermakna  lebih berperan dalam menurunkan kadar fibronektin dibandingkan MICT. Disimpulkan induksi L-NAME dapat menyebabkan hipertensi dan HIIT dapat  menurunkan berat badan, kadar angiotensin II, dan kadar fibronektin dibandingkan MICT. HIIT lebih berperan menurunkan risiko hipertensi dibandingkan MICT.
Molecular Pathway of Phytochemicals in Preventing Sarcopenia Sari, Desy Nofita; Goenarjo, Roman Ardian; Sianipar, Imelda Rosalyn
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 26 No. 03 (2025): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol26-iss03/621

Abstract

Sarcopenia, the age-related decline in muscle mass and function, poses a significant global health challenge. This systematic review synthesizes evidence from preclinical studies published between 2020 and 2025 to elucidate the molecular pathways through which phytochemicals prevent sarcopenia. Following PRISMA guidelines, a systematic search of databases identified 16 eligible studies. The analysis reveals that phytochemicals, including flavonoids, polyphenols, and botanical extracts, mitigate muscle atrophy by concurrently enhancing anabolic signaling via the IGF-1/PI3K/Akt/mTOR axis, suppressing proteolysis, improving mitochondrial biogenesis, and reducing oxidative stress and inflammation. Notably, combinatorial formulations and multi-target extracts demonstrate superior efficacy. The review concludes that phytochemicals represent a promising multi-targeted strategy against sarcopenia; however, this promising potential necessitates future validation through standardized clinical trials to establish efficacy and safety in human populations.
Co-Authors Ahmad Aulia Amirah Yusnidar Antoni, Marcel Gita Pratama Goenarjo, Roman Ardian Inna Rahmawati Iqbal Fasha Irena Ujianti Jalma, Monica Dwi Murti, Krishna Aditya Naibaho, Fitri Boru Neng Tine Kartinah Almuktabar Nisa Kartika Komara Nuraini Diah Noviati Nurhadi Ibrahim Nurul Paramita Patwa Amani, Patwa Putu Indah Paramita Adi Putri Radiana Dhewayani Antarianto Retno Lestari Robby Nuraditya Samuel Febrian Wijaya Santoso, Dewi Irawati Soeria Sophie Yolanda Trinovita Andraini Trinovita Andraini