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Ummy Mardiana Ramdan
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Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Published by Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medis Indonesia
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 5 Documents
 1 
Search results for , issue "Vol 5 No 2 (2024): In Progress" : 5 Documents clear
Antibacterial activity of coriander seeds extract (Coriandrum sativum) against the growth of Streptococcus mutans Kodariah, Liah; Amaliah, Eka Yuliani; Fadhillah, Tyas Ismi
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.149

Abstract

Background: Streptococcus mutans is the main cause of dental caries, previously known as part of the normal flora in the oral cavity which plays a role in the process of fermenting carbohydrates to produce acid, and causes tooth demineralization. Chemical-based medicinal products such as toothpaste and mouthwash. Continuous use of chemicals can cause side effects for users such as hypersensitivity, therefore an alternative treatment from natural ingredients such as coriander seeds which can inhibit bacterial growth is needed. Objective: This research aims to determine the lowest concentration of coriander seed extract in 0,75%, 1.5%, 3% inhibiting the growth of Streptococcus mutans. Methods: This study was conducted by soaking the test discs in coriander seed extract concentrations of 0.75%, 1.5% and 3%. each disc was inserted into the bacterial culture to be cultured, incubated and seen whether there was a clear zone or not. Result: Test results showed a clear zone at a concentration 1.5% with an average diameter of 1.5 mm, at concentration of 3% an average diameter of 3.7 mm and at concentration of 0.75% did not show clear zone. Conclusion: Based on the results of the study, the lowest concentration of coriander seed extract was obtained which was able to inhibit the growth of Streptococcus mutans bacteria, is at concentration of 1.5%, the diameter of the inhibition zone of coriander seed extract had weak antibacterial activity.
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN LIDAH MERTUA (Sansivieria tirfasciata var.laurentii) TERHADAP BAKTERI Klebsilla pneumonia Fitriani, Audiah; Sawitri, Nining Eka; Khatrina, Syahdapuan Dara; Nadifah, Fitri; Arisandi, Desto
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.217

Abstract

Background: Pneumonia is an acute infection of the lower respiratory tract characterized by coughing and shortness of breath caused by Klebsiella pneumoniae bacteria. Sansevieria contains flavonoids, saponins, alkaloids, and steroids known to have antibacterial properties that can destroy the bacterial cell walls.  Objectives: This study aimed to determine the antibacterial activity of Sansevieria leaf extract against the growth of K. pneumoniae.  Materials and Methods: Detection of antibacterial activity of Sansevieria was conducted with disc-diffusion test. The presence of antibacterial activity is indicated by the appearance of inhibition (clear) zone after applying the extract at a concentration of 60-100% to the culture medium. As a positive control, the antibiotic chloramphenicol was used.  Results: The higher the extract concentration, the higher the inhibition zone formed. The inhibition zone formed from extract concentrations of 60%, 70%, 80% is classified as medium (d = 8; 8.5; 9 mm). Meanwhile, the inhibition zones at concentrations of 90% and 100% are 10.5 and 13.5 mm and are classified as strong. The clear zone of the positive control was 29 mm (very strong). Conclusions: Sansevieria leaf extract has the potential to inhibit the growth of K. pneumoniae bacteria.
Optimalization of NaOH concentration in alkaline lysis method on quality and quantity of Candida albicans DNA Putri, Nasya Desinta; Prayekti, Endah
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.222

Abstract

Background: The concentration of NaOH in the alkaline lysis method needs to be optimized, especially when isolating DNA for its application in PCR which has not been maximized using the Candida albicans fungus which has a complex cell wall and is difficult to break. Thus, this optimization is expected to increase the efficiency of the alkaline lysis DNA isolation method in fungal species. Objective: The purpose of this studied was to determine the effect of optimizing the concentration of NaOH in the alkaline lysis method on the quality and quantity of Candida albicans DNA. Materials and Methods: This researched is experimental by performed DNA isolation used alkaline lysis method used NaOH concentrations of 1.5 N, 1.75 N, and 2.0 N and controlled (NaOH 0.2 N). Calculation of DNA quality and quantity using a nanodrop spectrophotometer. Results: The results showed the ordered of obtained the high to low DNA quality (A260/280) was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (1.86±0.44-0.95±0.18) while for DNA quantity was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (187.7±58.3-9.6±3.5 ng/?l). Conclusion: There is an effect of optimizing NaOH concentration on the lysis of fungal cell walls in the alkaline lysis method, namely increased the purity valued and concentration of sample DNA isolation results gradually. The use of 2.0 N NaOH concentration produced the best quality and quantity of DNA, namely 1.86±0.44 for DNA quality and 187.7±58.3 for DNA quantity.
Modification of CTAB incubation time in the isolation of DNA from Staphylococcus aureus Awaliyah, Arisma Putri; Prayekti, Endah
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.224

Abstract

Background: DNA was an essential component in organisms that regulates biological activity. DNA analysis began with isolating pure, highly concentrated DNA, often used the CTAB method to isolate DNA from bacteria. DNA quality affected by incubation timed and temperature as well as its purity. The right incubation timed could optimize the degradation of proteins in the cell wall and maximize the released of DNA from the cell. DNA purity is good if the 260/280 absorbance ratio valued is 1.8-2.0. Objectives: This studied aimed to obtained the highest concentration and quality of DNA by modifying the incubation time of CTAB used Staphylococcus aureus DNA. Materials and Methods: This studied uses quantitative methods with the typed in experimental researched. The isolation stage included cell lysis with CTAB buffer and proteinase-K, purification stage with chloroform: isoamyl alcohol (24:1), DNA precipitation stage with isopropanol. Results: The average result of DNA purity obtained from the incubation treatment for 10 minutes was 1.89, for 20 minutes incubation was 1.46, and for 30 minutes incubation was 1.23. The average DNA concentration obtained from the incubation treatment for 10 minutes was 153 ng/µL, for 20 minutes incubation was 78.2 ng/µL, and for 30 minutes incubation was 26 ng/µL. Electrophoresis results at 10 minutes incubation time clearly visible DNA bands, 20 minutes incubation time DNA bands looked faint, and 30 minutes incubation time is not visible DNA bands. Conclusions: Based on the results of the studied, modification of CTAB incubation time with an incubation time of 10 minutes is the result of the highest concentration and quality of DNA using Staphylococcus aureus DNA.
Evaluation of critical value report of laboratory results based on national quality indicators at Bima general hospital Pratiwi, Rahma Indah; Pujiyanto, Pujiyanto
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.235

Abstract

Background: To improve service quality and patient safety, Bima Regional General Hospital has set quality targets according to the national indicator. One such indicator is the national quality indicator for laboratory services, which aims to achieve a critical laboratory value report of 100%. Objectives: This research aims to evaluate the reporting of laboratory critical values by considering the achievement of predetermined quality indicator targets. Material and Methods: This research was carried out by collecting secondary data using the cross sectional design at Bima Hospital from January 2023 to March 2023 with a total sampling of critical value reports to the clinician or room nurses from all treatment rooms. Achievement of reporting time is calculated based on the percentage of timeliness of reporting critical value results compared to the total of all critical value reporting and is expressed as 'achieved' and 'not achieved'. Results: This research identifies the achievements of reporting that do not meet the target, and several obstacles and challenges that affect the efficiency and effectiveness of reporting. These findings indicate needs for improving coordination, communication and reporting procedures to support better achievement of quality indicator targets. Conclusions: The proposed improvement recommendations can help hospitals optimize the laboratory reporting process, thereby improving the quality of service and overall patient safety.

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