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Natural Sciences Engineering and Technology Journal
Published by HM Publisher
ISSN : -     EISSN : 28072820     DOI : https://doi.org/10.37275/nasetjournal
Core Subject : Health, Science, Social, Engineering,
Aerospace Engineering Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Electrical & Electronics Engineering Energy Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Social Sciences Transportation
Natural Sciences Engineering and Technology Journal (NASET Journal) concern with publishing the original research articles, review articles from contributors, and the current issues related to engineering, technology, and natural sciences. The main objective of NASET Journal is to provide a platform for international scholars, academicians, and researchers. It also aimed to promote interdisciplinary technology studies in Informatic Engineering, Electronica Engineering, Civil Engineering, Informatic System, Computer System, Architecture, and Natural Sciences in the world.
Arjuna Subject : Umum - Umum
Articles 61 Documents
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Mycotoxicological Profiling and Chromatographic Characterization of Retail Spices: A Mechanistic Evaluation of Microclimatic Packaging Dynamics on Aflatoxin Biosynthesis in Tropical Environments Khrisna Agung Cendekiawan; Linda Suci Anggraeni; Firdha Aprillia Wardhani; Rina Fitriana
Natural Sciences Engineering and Technology Journal Vol. 6 No. 1 (2026): Natural Sciences Engineering and Technology Journal
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/nasetjournal.v6i1.75

Abstract

Spices produced in equatorial tropical climates are highly susceptible to mycotoxin contamination. Aflatoxin, a carcinogenic secondary metabolite synthesized by Aspergillus flavus and Aspergillus parasiticus, poses a severe global public health threat by inducing hepatotoxicity and transversion mutations. This study investigates the prevalence of toxigenic fungi and quantifies aflatoxin analogues in retail culinary spices across varying microclimatic environments. An observational surveillance study was conducted using purposive sampling of onion, turmeric, and pepper matrices from traditional markets and modern supermarkets. Fungal characterization utilized Potato Dextrose Agar and Nash-Snyder media. Precise quantification of Aflatoxins B1, B2, G1, and G2 was executed via high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) and post-column derivatization. Mycological isolation confirmed pervasive colonization by Aspergillus flavus, A. niger, Penicillium spp., and Fusarium oxysporum. Method validation yielded excellent recovery (greater than 85%) and precision. Supermarket-sourced pepper (Sample C4) exhibited catastrophic hyper-contamination, with Aflatoxin B1 at 45.35 ppb and total aflatoxins at 99.35 ppb. Turmeric demonstrated intrinsic resistance, with maximum Aflatoxin B1 at 1.41 ppb. Non-parametric Spearman rank analysis revealed a significant positive correlation between trapped packaging micro-humidity and aflatoxin synthesis. In conclusion, modern non-vacuum packaging methodologies drastically elevate trapped micro-humidity, heavily driving the logarithmic growth and toxicological output of Aspergillus. Strict regulatory enforcement of storage humidity and water activity monitoring is urgently required in commercial retail sectors.

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