cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Darwin H Pangaribuan
Contact Email
journal.jaab@gmail.com
Phone
+6282183283777
Journal Mail Official
journal.jaab@gmail.com
Editorial Address
Griya Shanta Eksekutif P470 Lowokwaru, Malang, Indonesia 65141
Location
Kab. malang,
Jawa timur
INDONESIA
Journal of Agriculture and Applied Biology ( JAAB)
Published by Future Science
ISSN : -     EISSN : 27235106     DOI : https://doi.org/10.11594/jaab
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Agriculture and Applied Biology (J. Agric. Appl. Biol.) is striving to disseminate recent techniques and applications in agriculture and applied biology for researchers, students, and scholars in research institutes and universities worldwide. Journal focuses on crops, from seed to quality, growing under the tropical zones The journal publishes articles that report recent findings in the following areas: Seed Science and Technology Agronomy & Horticulture Agrotechnology Ecophysiology Crop Protection Soil Fertility & Organic Farming Land Use & Land Evaluation Sustainable Agriculture. Plant-Microbe Interactions Plant Biology Plant Biotechnology Agrobiodiversity Agroforestry Biosciences Biopharmaca Agroindustry Agricultural Engineering Postharvest Physiology & Technology Product Quality
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 118 Documents
 5   6   7   8   9 
Efficient Agrobacterium mediated genetic transformation of CRISPR/Cas9 construct using cotyledonary node explants of greengram Talakayala, Ashwini; Dokka, Narasimham; Baloji, Gugulothu; Ankanagari, Srinivas; Garladinne, Mallikarjuna
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.01

Abstract

An efficient protocol for generating transformed greengram plants by choosing prominent explant which is used to generate transformed plantlets for their survivability and also with high transformation efficiency. However, the crop production is reduced due to factors. In current work, we employed transgenic free approach using CRISPR/Cas9 tool for establishing transformation protocol. The CRISPR/Cas9 is getting momentum widely for developing transgene free crops. In this approach, guide RNA encoding for ~18-23 bp sequence that contains a PAM (protospacer adjacent motif) on either strand of DNA, where CRISPR incepts with a single guide RNA (sgRNA) to target genes and initiate excision of a complimentary strands through the Cas9 endonuclease. Explants’ effects on CRISPR/Cas9 construct transformation were studied. For transformation, three distinct explants cotyledonary node (CN- MSB5 +3% sucrose+ 0.5 mg/L BAP for CN), shoot tip (ST- MSB5 + 3% sucrose+ + 0.5 mg/L BAP + 0.01 mg/L NAA), and immature cotyledon (IMC-3% sucrose + 0.5 mg/L BAP + 0.1 mg/L NAA) were used. The prepared explants were infected with EHA105 strain of Agrobacterium tumefaciens harboring a binary vector of pMDC100 containing npt II gene as screenable marker and Cas9. The explants were selected on 50 mg/L kanamycin medium. The transformed plants were confirmed by PCR using npt II and Cas9 specific primers. Cotyledonary node explant was found to be more efficient for CRISPR/Cas9 transformation and also it promisingly showed transgene integration through PCR analysis having more transformation efficiency and can be used for the production of transgenic free greengram crop using CRISPR/Cas9 approach.
Effects of fermented banana pseudo-stem sap (Musa acuminata L.) on the growth and yield attributing characters of marigold variety Karma 555-Orange Mehta, Aman; Rijwan Sai; Rakesh Bhujel; Navina Yadav; Sneha Khanal
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.02

Abstract

The high cost of marigold production through the application of synthetic fertilizers and micronutrients is a major concern for Nepalese farmers. Every year, the nutrient-rich banana pseudo-stems are wasted after harvest. Utilizing the banana’s pseudo-stem for increasing marigold productivity through sustainable agricultural practices, an experiment was conducted to determine the optimum concentration of banana pseudo-stem sap (BPS) to be applied by foliar spraying to marigold variety (Karma 555 – Orange). The BPS was mixed and enriched with different ingredients, such as cow urine, cow dung, neem leaf, green gram leaf, pulse flour, vermin-liquor, jaggery, and fermented curd. The mixture was incubated under anaerobic conditions before applying the foliar spray. The study was carried out in a completely randomized block design, with three replications and seven treatments. The treatments were 0.5 % BPS, 1 % BPS, 2 % BPS, 3 % BPS, 4 % BPS, 5 % BPS, and the control. Data were recorded at 50, 70, and 90 days after transplantation (DAT). Among the treatments, the 3 % BPS solution showed significant effects on most of the growth parameters: plant height (85.07 cm), plant spread (63.47 cm) at 5% level at 90 DAT, and yield attributes such as the number of flowers per plant (74.00) at 5% level, individual flower weight (8.10 g), individual flower diameter (7.55 cm), and flower yield (597.25 g plant-1) at 1% level of significance respectively. It was found that growth and yield attributes increased with increasing concentrations of BPS up to 3 % and decreased thereafter. According to the results, foliar spray of marigold with 3 % BPS (enriched solution) resulted in the highest yield and yield components of marigold in the Chitwan area which could be an effective alternative to synthetic hormones for enhancing the growth and quality of marigolds under field conditions.
Isolation and characterization of endophytic fungi associated with Ephedra alata (macroscopic and microscopic characterization) Fatima, Zair; Lakhdar, Mebarki
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.03

Abstract

Ephedra alata, known in Algeria as Alanda, is a medicinal plant belonging to the Ephedracea family, it looks like a shrub without leaves. This species can grow in semi-arid and desert conditions thanks to its high tolerance to water shortage. It is used in traditional medicine for its therapeutic properties, in particular to treat the bronchi, the circular system, digestive system disorders, kidneys, oedema, fever, headaches, allergies, and to relieve asthma attacks as well as bacterial and fungal infections this is due to its production of secondary metabolites with a wide range of biological activities. On the other hand, This plant represents a host that allows endophytic fungi to feed, protect and spread; in return, this plant also benefits from certain advantages of endophytes. Despite its importance, no studies on its endophytic fungi has been conducted to date. This study aimed to isolate and identify the fungal endophyte community associated with the plant Ephedra alata growing in arid environments. the harvest of the Ephedra alata plant (aerial part) was made in the region of Bechar (southwest algeria). Isolation and purification of fungal strains were carried out on PDA medium supplemented with lactic acid. The identification of the isolated strains was made on the basis of morphological and cultural criteria. Ephedra alata presents a wide diversity of fungal species with a high load. A total of twelve fungal strains were isolated and the predominant genera were Aspergillus, then Penicillium, Mucor, and unidentified structures in small percentages. The most common phylum of fungal genera identified was Ascomycota, followed by Zygomycota and small percentages of unidentified structures (SNI).
Application of ι-Carragenan/Agarose Hydrogel as Super Adsorbent Hydrophilic Polymers Natural Seed Coating for Improving Tobacco Seed Germination Under Drought Stress Arum, Ayu Puspita; Setiyono
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.04

Abstract

Seed storage for a long time causes seed deterioration. Reduction of germination rate can cause abnormal growth of the seedling, which impacts the yield and quality of tobacco plants. Seed coating is an additive that coats the seeds with certain ingredients such as pesticides, and nutrients that are glued to a binding material for improved seed performance without changing its origin's form. Hydrogel seed coating is also natural and environmentally friendly which is suitable for organic and sustainable farming practices. In countries with worse droughts and depending on natural plants, natural seed coating using agarose and Carragenan is very well applied. Carrageenans regulate many of the metabolic pathways involved in nitrogen and sulfur absorption, making it easier to grow plants by regulating a variety of metabolism processes including photosynthesis as well as an ancillary pathway. In conditions of drought stress, salicylic acid treatment may have a beneficial effect on plant resistance. In plants, salicylic acid regulates the growth of endogenous cells and plays an important role in abiotic stress signal transduction. The present field experiment was conducted to evaluate the effectiveness of ι-Carragenan/Agarose hydrogel as super adsorbent hydrophilic polymers natural seed coating with 3 type combination of ι-Carragenan and Agarose (1:1; 1:2 and 2:1)% w/w containing salicylic acid 0.15% w/w to improve sprouting speed index, sprouting percentage, fresh seed mass and seed vigor index of tobacco seeds which are 3 variety storage time (13 years, 9 years and 3 years) under drought stress condition (40% moisture levels). The results revealed that application of ratio ι-Carragenan/Agarose 1:2 and 2: 1 % w/w containing salicylic acid 0.15% w/w recommended of Super Adsorbent Hydrophilic polymers Natural Seed Coating formulation enhanced of sprouting speed index, germination percentage, and seed vigor index of 2 variety storage time of tobacco seed (13 years and 9 years).
AMMI and GGE biplot analysis of yield performance of wheat genotypes under irrigated, heat stress and heat drought environments Timalsina, Bibisha; Ghimire, Sweksha; Roka, Puja; Poudel, Rashmi; Sapkota, Sushma; Bhattarai, Kriti; Aryal, Anil; Ganesh , G. C.; Neupane, Kapil; Gautam, Pratik; Pariyar, Manoj Kumar; Sharma, Surakshya; Chaudhary, Eishaina; Poudel, Mukti Ram; Bhandari, Radhakrishna
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.09

Abstract

Wheat is the third most important cereal crop in Nepal. The impact of global warming is threatening global wheat production and food security. The terminal heat stress reduces the grain quality of wheat. However, the drought is affecting more than 15% of global wheat productivity. To find out the stable and high- yielding wheat genotype the experiment was carried out in Rupandehi, Nepal with twenty genotypes under three different environmental conditions namely heat drought, heat stress and irrigated in an alpha lattice design with two replications in each environment. The AMMI (Additive mean effect multiplicative interaction) biplot analysis shows differences in 20 different genotypes in terms of yield and stability. The analysis of variance model showed the share of GE (genotype and environment) interaction in the variation in grain yield of twenty wheat genotypes. The grain yield of genotype varied significantly with environmental impact (p ). The AMMI stability value (ASV) examined NL1387 as the most stable line. The tested environments were discriminative for genotype and showed negative correlation between them. The GGE biplot analysis was conducted to find out the best performing line under different environments and the stable line in diverse environments. The NL1420 was found stable genotype in all three tested environment. The NL1376 line is most ideal ranking first in the ranking biplot. The mean versus stability model indicated NL1369 and NL1376 as elite genotypes and NL 1404, BL4919 and NL1387 can be recommended as new cultivars.
Growth and yield of maize applicated by Rizhobium spp. from legume and non-legume rhizosphere Widodo, Tirto Wahyu; Muhklisin, Ilham; Nugroho, Setyo Andi; Wardana, Rudi; Ummah, Umi Safitri Alifia
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.05

Abstract

Rhizobium spp can affect plant growth in non-legume crops directly by synthesis of phytostimulator and solubilization of inorganic phosphate. However, the ability of Rhizobium spp from legume and non-legume rhizosphere to trigger the growth of non-legume crops is different. This research aims to analyze the ability of Rhizobium spp isolates to produce IAA hormones and phosphate solvent and determine the response of maize with the application of Rhizobum spp from the legume and non-legume rhizosphere. This study was carried out in two stages, where the first stage at Bioscience Laboratory Politeknik Negeri Jember, while the second stage at Kaliurang field, Jember, Indonesia (altitude 146 m asl, temperature 21°C - 34°C, and soil type was incepstisol) from September 2022 to January 2023. The field experiment was arranged in a complete randomized design (crd) with the application of Rhizobium spp isolates from various rhizosphere as a treatment consisting of without Rhizobium spp (control), maize-rhizosphere isolate, rice-rhizosphere isolate, soybean-rhizosphere isolate, edamame-rhizosphere isolate, and peanut-rhizosphere isolate. Every treatment was replicated four times. The results showed that Rhizobium spp isolates from legume and non-legume rhizospheres can synthesize indole acetic acid and solubilize phosphate. This condition was indicated by the solubilized phosphate content in the planting medium, which was higher in the application of Rhizobium spp compared to the control. Inoculation of Rhizobium spp from several rhizospheres showed a significant effect on plant height, stem diameter, ear weight without husks, and ear dry weight compared to control. These bacteria are able to trigger the growth of maize through direct and indirect mechanisms. In addition, the plant height that was treated with maize-rhizosphere Rhizobium spp was better than rice. It is suspected that Rhizobium spp from the maize rhizosphere is more adaptable when applied to growing media for maize crops, so that it can increase plant height.
Pimpinella anisum essential oil: A natural approach to amplifying fertility and reproductive well-being in the Saida Region Ammam, Abdelkader; Belmamoun, Ahmed Reda; Zemour, Hafidh; Ammour, Fatima; Kaid, M’hamed; Villemin, Didier; Soufan, Walid; Belhouadjeb, Fathi Abdellatif
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.10

Abstract

Infertility poses a significant challenge within the sheep population, hindering herd regeneration. Both rams and ewes are susceptible to infertility, prompting our objective of enhancing reproductive performance through the utilization of essential oils as a means to mitigate this issue. Essential oils have long been acknowledged for their medicinal properties and have been extensively employed in traditional medicine. Some essential oils have been reported to exert effects on male reproductive functions in both animals and humans.Pimpinella anisum, scientifically known as green anise, is a medicinal and aromatic annual plant renowned as one of the oldest medicinal herbs and widely used as a spice. In Algeria, essential oils extracted from Pimpinella anisum are consumed on a wide scale; however, their potential in enhancing ram fertility has remained unexplored.To improve the fertility of the Rembi breed sheep population, rams were randomly selected for this study. These rams underwent various experiments to evaluate their response to different concentrations (0, 0.5, and 1 mm/ram) of essential oil obtained from anise seeds using the hydro-distillation extraction method. Furthermore, the study encompassed an assessment of their key zootechnical characteristics over a one-year period.
Aspects of the ecosystem services threatened by desertification in Algerian steppe rangelands: concepts, status and stakes Habib , Benbader; Saadaoui, Mohamed; Boumakhleb, Abdallah; Brahimi, Malika; Doghbage, Abdelghafour; Djoughlafi, Adel; Zemour, Hafidh; Belhouadjeb , Fathi Abdellatif
Journal of Agriculture and Applied Biology Vol 5 No 1 (2024): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.05.01.01

Abstract

This study focuses on the imminent threat of desertification within Djelfa's steppe grazing lands, emphasizing its implications for land management and the sustainability of livestock practices. The methodology adopted employs a stringent approach that commences with a precise definition of desertification as a critical risk. It then proceeds to thoroughly assess the susceptibility of the local ecosystem to this phenomenon and delineates its consequential impact on both the human inhabitants and the surrounding environment. This comprehensive analysis effectively contextualizes human activities within the sphere of desertification's influence. Methodologically, the study employs a multidimensional framework to categorize the array of environmental goods and services rendered by these grazing lands. By identifying the beneficiaries associated with each service, the research aims to elucidate the complex threat posed at various levels. Crucially, the findings highlight the severe jeopardy that desertification imposes, not only endangering essential resources vital for extensive livestock production but also triggering a decline in invaluable environmental goods pivotal for the sustainability of the ecosystem and activities supporting human welfare. Statistically substantiated through an integration of diverse methodologies such as field surveys, satellite imagery analysis, and stakeholder consultations, this study validates the correlations between desertification and the degradation of ecosystem services. It provides empirical evidence showcasing the gradual decline of grazing lands, thereby compelling an urgent call for intervention strategies. In summary, this research underscores the urgent need for holistic strategies to mitigate the adverse effects of desertification. Its findings provide critical insights into the complex dynamics between human activities, ecosystem vulnerabilities, and the looming threat of desertification. It emphasizes the necessity for immediate collective action and sustainable resource management practices to safeguard ecosystems, ensure long-term sustainability, and protect the well-being of communities in Djelfa and beyond.
The efficacy of Acorus calamus L. crude extract formulated in bentonite nanoparticles against Crocidolomia pavonana (Lepidoptera:Pyralidae) Purwatiningsih; Indarti, Dwi
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.07

Abstract

The prospect of A. calamus extract to be botanical insecticides to control the insect pests is very promising. The insect control properties of the A. calamus are predominantly to have β-asarone, saponin and flavonoid. However, there are some factors limiting their efficacy. Short release rate at the point contact, the inherent volatility and vulnerability to oxidation and ultra-violet light are causing phytochemical changes during the application. Thus developing nanotechnology to increase their efficacy studied in this research. To develop the plant extract in nanoformulation, experiment on adsorption and desoption capacity were carried out with Acorus extract treated bentonite substrate analysed by GC techniques. In addition, to investigate the effectiveness of the plant extract treated bentonite were evaluated on Crocidolomia pavonana. Third instar larvae were used in the evaluation of antifeedant effect by using no choice methods. The results showed that Bentonite nanoparticles was effective in controlling the release of a bioactive element. Purifed clay bentonite for getting Na-bentonite increased the specific surface area of material therefore increased the adsorption site on the clay layer of the material. Crude extract of A.calamus can dissolve well in combination of water and organic solvents. Desorption experiments proved that Na-bentonite released more slowly than raw clay bentonite. Purification of bentonite to Na-bentonite resulted the changing of phytochemical properties bentonite which lead to an increase in the adsorption capacity of bentonite. In term of antifeedant evaluation based on damage to cabbage leaves seedling, the least damage was observed on leaves with nanoparticle plant extract formulation. The antifeedant index of A. calamus in nanoparticles formulation showed 25% higher that untreated cabbage plant seedling. The glasshouse trial was conducted to evaluate their efficacy on cabbage seedling. However, the brown leaves or phytotoxic effect has been found by the time the extracts nano formulation has been sprayed.
Bio-organic approach for monitoring the Plant growth for Sustainable agriculture Narula, Gita Batra; Soni, Kiran; Vadakan, Alka B; Sharma, Shweta; Notnani, Prerna; Ghai, Nancy; Gandhi, Kanika
Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.04.02.06

Abstract

Agriculture plays a critical role in sustaining life and driving the economy of India. The idea of sustainable agriculture is a holistic approach to meet the demands of food supply while protecting our environment. Through various sources, it has been found out that the country is running out of the main reservoirs of water to irrigate the fields. The present study investigated the possibility to prepare different natural hydrogels using Chia seeds, Flax seed, Gond katira, Arrowroot powder, Tapioca, Agar-agar, Corn starch, Gelatin, Aloe vera, and their comparison with a synthetic hydrogel used in sanitary napkins. On successful formation of natural hydrogels and getting valuable results in their water holding capacity, the work continued to test their potential on growth and development of seeds of two plants: Moong beans (Vigna radiata) and Fenugreek (Trigonella sp.) along with bio-organic fertilizers prepared from onion, garlic and banana respectively. Seeds were sown in soil less media (Coco-coir) and the study of various parameters like phytotoxicity test, seed germination, water consumption, number of leaves, root and shoot length indicated promising results in the establishment and healthy growth of the seedlings. Use of natural biodegradable hydrogels therefore may prove as an easy, low cost and eco-friendly way to establish the seedling development and subsequent productivity at the commercial level under conditions of water scarcity for sustainable agriculture.

Page 7 of 12 | Total Record : 118

 5   6   7   8   9 

Filter by Year

2020 2026


Reset
Filter By Issues
All Issue Vol 7 No 1 (2026): Journal of Agriculture and Applied Biology Vol 6 No 2 (2025): Journal of Agriculture and Applied Biology Vol 6 No 1 (2025): Journal of Agriculture and Applied Biology Vol 5 No 2 (2024): Journal of Agriculture and Applied Biology Vol 5 No 1 (2024): Journal of Agriculture and Applied Biology Vol 4 No 2 (2023): Journal of Agriculture and Applied Biology Vol 4 No 1 (2023): Journal of Agriculture and Applied Biology Vol 3 No 2 (2022): Journal of Agriculture and Applied Biology Vol 3 No 1 (2022): Journal of Agriculture and Applied Biology Vol 2 No 2 (2021): Journal of Agriculture and Applied Biology Vol 2 No 1 (2021): Journal of Agriculture and Applied Biology Vol 1 No 2 (2020): Journal of Agriculture and Applied Biology Vol 1 No 1 (2020): Journal of Agriculture and Applied Biology More Issue