Berkala Penelitian Hayati
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
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<![CDATA[PHYTOCHEMICAL TESTED AND IN VITRO SCREENING ANTI- MALARIA ACTIVITY OF BELILIK (Brucea javanica (L)). MERR AGAINST Plasmodium falciparum ]]>
<![CDATA[Henny Helmi]]>;
<![CDATA[Idha Susanti]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/128
<![CDATA[Screening of anti-malaria compound from nature is one of important thing to cure malaria disease especially in Bangka Belitung province. One of plant that as used as traditional medicine to cure malaria is Belilik (Brucea javanica (L.)Merr. The aim of this study was to investigate the present of some kind of biochemical compound, and to evaluate in vitro anti-malaria activity of ethanol extracts of Bruce javanica fruits and roots against Plasmodium falciparum. A visual method was allowed to evaluate the in vitro anti-malaria activity of the extracts against P. falciparum. The number of parasites per 5,000 erythrocytes on thin Giemsa stained smears was calculated microscopically. IC50 values were determined by probit analysis of SPSS 13 program. The result showed, that the ethanol extract of Belilik contained some biochemical compound such as alkaloid, flavonoid and phenol in fruits and alkaloid, phenol and saponin in roots. The anti-malaria activity of ethanol extracts of Brucea javanica fruits and roots on P. falciparum were very effective as IC50<0,01 μg/mL. The highest inhibition of 100 μg/mL of Brucea javanica fruits and roots were 5.95% and 16.99% respectively. Brucea javanica could inhibit Plasmodium falciparum in vitro with very small dosage of extract and very potential to develop as malaria medicine.]]>
<![CDATA[ACCLIMATIZATION OF PLANT COLLECTION FROM EAST NUSA TENGGARA EXPLORATION (EGON FOREST, MUTIS MOUNT, AND CAMPLONG PARK) AT PURWODADI BOTANIC GARDEN ]]>
<![CDATA[Trimanto Trimanto]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/130
<![CDATA[Acclimatization is treatment to adapt the plants material from forest exploration. This activity determine the survival of plants material in nursery at Purwodadi Botanical Garden. Endemic and rare species from East Nusa Tenggara are expected to survive in the Purwodadi Botanic Garden plant collection. This research is aimed to observe acclimatization procces in Purwodadi Botanic Garden and to know the species of plant in East Nusa Tenggara which they can’t adapt in the Purwodadi Botanical Garden. The research was conducted at nursery unit with observational methods. The results show that 50% of 209 accession plants from East Nusa Tenggara can’t survive at Purwodadi Botanic Garden. It consists of 60 accession plants from Egon, 20 accession plants from Mutis, and 18 accession plants from Camplong.]]>
<![CDATA[THE EFFECT OF SALT TOLERANT NITROGEN FIXING BACTERIA ON THE GROWTH OF PADDY RICE (Oryza sativa. L) ]]>
<![CDATA[Suliasih Suliasih]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/131
<![CDATA[Nitrogen fixing bacteria (Azospirillum and Azotobacter) were isolated from coastal mangrove in Pulau seribu. The aims of this experiment was to find out isolates of nitrogen fixing bacteria which were tolerant to high salinity. The isolates can be used as a biofertilizer to support coastal agriculture. A total of 28 isolates (14 isolates of Azospirillum and 14 isolates of Azotobacter) were tested their tolerance to salt by growing them in their respective media containing 1% 2% and 3 % NaCl,. Salt tolerant isolates obtained, then used as inoculants in paddy. The experiment laid out factorial based randomized complete block design which was comprised of 7 fertilizer treatments and 5 watering treatments with 5 replicates for each treatment at green house of Microbiology Division, Research Center of Biology, Indonesian Institute of Sciences. The first factor was fertilizer treatments 1. Compost + mixed bacteria (K1), 2. Control without inoculant, 3. Compost, 4. NPK, 5. Compost + NPK + mixed microbial, 6. Azotobacter isolates; 7. Azospirillum isolates. The second factor was watering treatments, plant was watered by : 1. freshwater, 2. freshwater + sea water at mixture ratio 1:1, 3. Sea water, 4. sea water + 2% NaCl (20 g NaCl / l), 5. freshwater + 5% NaCl (50 g NaCl / l). The result showed that there were 9 isolates of Azospirillum and 4 isolates of Azotobacter which were tolerant to grow at media with 3% NaCl. The green house experimental result revealed that the plants were treated with bacteria can survive up to the level of salinity 12.43 dS-1m.]]>
<![CDATA[ANALYSIS OF GENETIC VARIABILITY IN SOURSOP (Annona muricata L.) POPULATIONS FROM CENTRAL JAVA AND EAST JAVA BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER ]]>
<![CDATA[Suratman Suratman]]>;
<![CDATA[Ari Pitoyo]]>;
<![CDATA[Sri Mulyani]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/132
<![CDATA[The objective of this research was to determine genetic variability of the soursop (Annona muricata L.) populations from Central Java and East Java based on RAPD markers. Leaves of 40 individuals were collected from 4 soursop populations in Central Java and East Java, include : Sukoharjo, Karanganyar (Central Java), and Ngawi, Pacitan (East Java). Genomic DNA was extracted from the leaves by the CTAB extraction procedure with some modifications. A total of 15 RAPD primers were purchased from commercial source and tested to find specific diagnostic markers for each individuals by RAPD-PCR. The measurement of soursop population genetic distance was based on similarity coefficient using method of Group Average Clustering and Unweight Pair Group Method Arithmetic (UPGMA) of NTSYS program version 2.02i. Results showed that each soursop population collected from different localities seemed have variability in RAPD profiles by using different primers. Four RAPD polymorphic primer was selected from 15 RAPD primers, namely A18, A20, P10 and P11. A total of 58 bands produced, varying from 9 to 20 bands per primer. The selected four RAPD primers produced 57 polymorphic bands, whereas polymorphism for each primer ranged from 95 % to 100 %. Dendrogram indicated that four soursop populations tend to segregate form two separated clade. The sample collected from Sukoharjo formed a separate cluster while the sample collected from Ngawi, Pacitan and Karanganyar grouped together in other cluster and diverged from population Sukoharjo.]]>
<![CDATA[THE DYNAMICS OF 1.3-B-D-GLUCAN GIVEN IN THE FORM OF AGARICUS BLAZEI MURILL POWDER TO THE BODY OF WHITE MICE Sprague Dawley AND EFFECT OF RENAL AND HEPATIC HISTOPATHOLOGY ]]>
<![CDATA[Misgiati Misgiati]]>;
<![CDATA[Umie Lestari]]>;
<![CDATA[Aloysius Duran Corebima]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/133
<![CDATA[Dynamics of compound 1,3-β-glucan in Agaricus Blazei Murill (ABM) powder and the effect of renal and hepatic histopathology performed on 24 male rats aged 2 months, were randomly conducted in three treatments there are control, ABM powder, and the pure compound 1.3-β-glucan for 28 days. Provision of treatment given in the morning and then 1, 3, 8, 12, and 24 hours after administration of treatment blood was collected, while for urine and feces were collected every 8 hours. Giving treatment was continued until day 28. The results showed that the levels of 1,3-β-glucan in pure compounds is higher than the Agaricus Blazei Murill (ABM) powder. The effects on the kidneys and liver histopathology showed that cells necrosis more on pure compounds from the mushroom powder gods, both the kidneys and liver. This study shows that the use of pure compounds to be more careful than Agaricus Blazei Murill (ABM) powder in determining the doses used.]]>
<![CDATA[EFFECT OF CALCUSOLTM ON CuZnSOD EXPRESSION IN MICE RENAL OF NEPHROLITHIASIS MODEL ]]>
<![CDATA[Arief Azhary]]>;
<![CDATA[Sri Widyarti]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/134
<![CDATA[This study aimed to determine the effect of traditional medicine, CalcusolTM, on number of cells expressing CuZnSOD in mice renal of nephrolithiasis model. Eight weeks old Swiss strain male mice (Mus musculus) were divided into five groups: (A) control, (B) nephrolithiasis, (C) CalcusolTM, (D) nephrolithiasis & CalcusolTM, and (E) nephrolithiasis & CalcusolTM simultaneously. Nephrolithiasis was induced by applying porang tuber (Amor- phophallus muelleri) flour 0.06 mg/g of body weight during 3 months. CalcusolTM is traditional medicine, made of tempuyung leaves extract with Saccharum lactis as additional substance. The dosage for CalcusolTM treatment was 3.3 mg/g of body weight. After 3 months treatment, the mice were killed by neck-dislocation, the kidneys were isolated and prepared for paraffin histology. CuZnSOD was analyzed by immunohistochemistry (IHC), using rabbit policlonal antibody anti-SOD1 (Bioss, bs-1079R) as primary antibody. Tissues were observed under Olympus BX51, 400x mag- nification. Images were documented with Olympus Digital Camera DP20. The histology images were analized in Immunoratio software online (URL: http://153.1.200.58:8080/immunoratio/) to receive the percentage of number of cells expressing CuZnSOD. The result showed that CalcusolTM administration could decrease number of cells expressing CuZnSOD in kidney significantly (P<0.05). It was supposed that antioxidant content in CalcusolTM could scavenge ROS directly with no induction of CuZnSOD production in cell.]]>
<![CDATA[VEGETATION SPECIES ABUNDANCE IN MANGROVE ECOSYSTEM OF PASIR MENDIT AT BOGOWONTO LAGOON KULON PROGO YOGYAKARTA ]]>
<![CDATA[Maizer Said Nahdi]]>;
<![CDATA[Ardyan Pramudya Kurniawan]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/135
<![CDATA[This research was conducted on Pasir Mendit, Bogowonto Lagoon. Pasir Mendit, Bogowonto Lagoon is one of unique beaches in Indonesia, with sand du-nes that protecting the beach from Indian Ocean €™s pounding waves. The purpose of this research is to shows the relation between the environmental fac-tors and the abundance of vegetations in the mangrove €™s ecosystem. Quadrates method were used for collecting the data, by making several plots of the study of growth forms. Connonical Correspondence Analysis (CCA) were used to analyze the correspondential relationship between vegetation and the environmental factors. The result of this research shows that there are 19 species of mangroves and its associates and unity. All of the locations are colo-nized by Acanthus ilicifolius bushes and Derris heterophylla woody climber, which is the r-strategy, with high level of density. Sonneratia alba and Rhi-zophora mucronata is the dominating species in all locations with varied abundance and importance value. This would be linked with the relatively high concentration of C organic, NO3 and SO4. The presence of Acanthus ilifolius and Derris heterophylla shows that the mangrove ecosystem of Men-dit has suffered from destructions. With the existence of mangrove in Bogowonto Lagoon, the place is suggested to be a conservation location model for other lagoons in Yogyakarta and Central Java.]]>
<![CDATA[DIVERSITY OF BAMBOOS AROUND SPRINGS IN MALANG EAST JAVA ]]>
<![CDATA[Solikin Solikin]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/136
<![CDATA[Bamboos have important roles to people in the villages area. They are planted and used by the people for making houses, food, buckets, fences, ropes, fuels, musical instruments and plaits. The root distribution of Bamboos is large and fibrous, also the growth of their new clumps is ascendant so the Bamboos has good potency for water and soil conservation on river banks, around the springs, hillsides and scarps. The survey to invent the bamboos growing around the springs was conducted in Singosari, Lawang, Karangploso, Dau and Lowokwaru Malang East Java in May 2009. The Bamboos invented at 0-100 m from the springs. The results Showed that there were four Bamboos founded around the springs namely Bambusa blumeana,Dendrocalamus asper, Gigantochloa atter and Gigantochloa apus. Dendrocalamus asper was the most dominant species founded around the springs with relative frequency, relative density and important value index is 45.83 ; 58.49 and 104.32 respectively.]]>
<![CDATA[CORAL REEFS DIVERSITY IN GILI GENTING ISLAND SUMENEP MADURA ]]>
<![CDATA[Fathorrohman Fathorrohman]]>;
<![CDATA[Romaidi Romaidi]]>;
<![CDATA[Bayyinatul Muchtaromah]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/137
<![CDATA[Coral reef ecosystem is the most threatened ecosystem among marine ecosystem in the world due to the combination of anthropogenic and natural disturbances. More research is needed to be monitor ed and assess coral reef ecosystems, which will be used to fin d understanding of the ecological integrity and further improvement of the protection strategy in the future. This re search was aimed to know the diversity of coral reef diversity at Gili Genting Island Sumenep Madura and evaluate the condition of coral reef ecosystem based on cover the percentage. Line intercept method was used to understand coral reef diversity and its condition in the desired observation station. The result revealed that 9 families, 22 genera and 4 5 species of coral have been successfully found, in which Acropora is the most common genus found in in Gili Genting Island . The cover percentage analysis also indicated that the condition of coral reef ecosystem in Gili Genting Island could be classified to very bad category where th e total average of cover percentage is 12.55%. These findings provide the prelim inary information about the condition of coral reef ecosystem in small i sland that might be useful for the future integrated management based on ecological perspective.]]>
<![CDATA[EFFECT OF CALCUSOL TO REDUCE THE CALCIUM CRYSTAL RETENTION IN KIDNEY EPITHELIAL CELLS MODEL OF NEPHROLITHIASIS ]]>
<![CDATA[Ahmad Soni]]>;
<![CDATA[Moch. Sasmito Djati]]>;
<![CDATA[Sri Widyarti]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/138
<![CDATA[Kidney stones is a disease that characterized by a disturbance in the bladder. The main constituent of kidney stones namely Calcium Oxalate Monohydrate (COM) crystals. The presence of a COM crystal adhesion to renal tubular cells, will initiate the internalization which will further lead to the formation of crystals retention in the kidney. In Indonesia, there are many herbal products are considered able to cope the complaints due to the kidney stone disease. One of the herbal product is Calcusol „¢, which is the main constituent of those herbal product was the leaf extract of tempuyung. This study observed the effectiveness of Calcusol „¢ in reducing crystals retention that was formed in kidney epithelial cells model of nephrolithiasis. The result showed that Calcusol „¢ is able to reduce the average number of calcium crystals retention in the renal epithelial cells. It indicate that Calcusol „¢ has the ability to reduce crystals retention that already formed in renal epithelial cells. Furthermore, the results of this study are expected to be one of the considerations for further research on the potential of overcoming Calcusol „¢ in kidney stone disease]]>
