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Contact Name
Sutiman Bambang Sumitro
Contact Email
berkalahayati@yahoo.com
Phone
+62341570631
Journal Mail Official
wulidanisa@berkalahayati.org
Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia
Location
Unknown,
Unknown
INDONESIA
Berkala Penelitian Hayati
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Articles 507 Documents
POLA PEWARISAN SIFAT DAYA HASIL KACANG TANAH HASIL PERSILANGAN cv. KELINCI DAN US 605 DALAM KONDISI TERCEKAM KEKERINGAN Adisyahputra; Sudarsono; K. Setiawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/289

Abstract

The aim of this research was to examine the yield characters of peanut in drought stress. The experiment was conducted by using of peanut cv. Kelinci that is sensitive genotype as female parent and US 605 which is tolerant genotype as male parent, including the F2 population F1 offspring from hybrid cv. Kelinci (P1) with US 605 (P2). The number of pods, amount of filled pods, dry weight of pods, dry weight of seed, the number of seeds, are measured during harvest. Expression of all yield characters were influenced by genetic factor, except the variable weight of seed. All of yield show the quantitative and qualitative characters. The result analysis of Mendelians genetic yield showed all characters were controlled by 2 until 3 gene with the dominant and resesif epistasis combination pattern. Result from joint scalling test analysis showed all variable were not only determined by additive and dominant influence but also by genetic interaction. Additive influence with the high narrow sense heritability most of all variables together can give opportunity to obtain tolerant peanut line with heigh yield.
SKRINING UNTUK TOLERANSI TERHADAP STRES KEKERINGAN PADA 36 VARIETAS KEDELAI PADA FASE PERKECAMBAHAN Wahyu Widoretno
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/290

Abstract

Water stress due to drought is the most significant abiotic factor limiting crop growth and development. Drought stress inhibit germination and seedling growth, and extention of inhibition in germination under drought stress condition is varying in varieties. The previous research result showed that the differences in germination response under drought stress can be used as early test for screening to drought tolerance in plants. The objectives of this experiment were to evaluate the germination response of 36 soybean varieties under drought stress simulated by polyethylene glycol (PEG), to know the drought-stress tolerance level of 36 soybean varieties based on germination response in medium containing PEG, and to identify drought tolerant varieties. Soybean seeds were germinated in sand medium containing several PEG (6000) at concentration 0%, 15% and 20% which equivalent to 0, -0,41 and -0,67 MPa during 5 days. Germination responses were observed by calculating vigor index, germination percentage, lateral root number and measuring hypocotyl and root lenght and seedling dry weight. The level of drought stress tolerance of soybean varieties were evaluated based on index of reduction and drought sensitivity on germination variables in stres and non-stres conditions. The research result showed that drought stress on germination media simulated by PEG decreased vigor index, germination percentage, lateral root number, hypocotyl and root lengths and dry weight of seedling. The inhibition of germination increased with reduction of water potential. The water potential -0,41 MPa inhibited vigor index and hypocotyls length in all tested varieties, but inhibited germination percentage, lateral root number and dry weight of seedling in some varieties. However, decreasing of water potential -0,67 MPa inhibited almost germination variables in all varieties, except root length. Each tested soybean variety showed differences of germination response to drought stres. Varieties of Dieng, Tidar and Sibayak showed less inhibition than others, but Anjasmoro, Burangrang, Galunggung, Kipas Putih and Tambora showed more inhibition in seedling growth. Based on index of reduction and drought sensitivity on germination variables, Dieng and Tidar were identified as tolerant varieties, while sixteen varieties as medium tolerant and seventeen varieties as sensitive ones.
POTENSI PHOTODINAMIK INAKTIVASI Staphylococcus aureus DAN Vibrio cholerae DENGAN ENDOGEN PHOTOSENSITIZER PADA PENYINARAN LED BIRU (430 ± 4) nm DAN MERAH (629 ± 6) nm Suryani Dyah Astuti; Djoni Izak R.; Ni’matuzahroh; M. Zainuddin; Suhariningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/291

Abstract

Photodynamic Inactivation (PDI) is bacteria inactivation method with using light and bacteria porphyrin photosensitizer.The combination of light and photosensitizer with suitable spectrum can promote photosensitization process and then cause bacteria photodamage. This research was laboratory experiment, to analyze photodamage potency of Gram positive bacteria Staphylococcus aureus and Gram negative Vibrio cholera with combination of endogen photosensitizer and LED exposure (blue LED (430 ± 4) nm and red LED (629 ± 6) nm)) on Pulse Width Modulation (PWM) 75% and time duration 30 minutes. The viability of bacteria had been counted after 48 hours incubation on temperature 37oC by using Total Plate Count (TPC). Result of this research showed that blue LED exposure (430 ± 4) nm had potency to decrease 70% Staphylococcus aureus and 50% Vibrio cholera bacteria colony forming unit. Red LED (629 ± 6) nm exposure decreased 22% dan 3% colony forming unit. So blue LED exposure had big potency to bacteria inactivate.
IDENTIFIKASI KARAKTER MORFOLOGI NEMATODA SISTA PADA TANAMAN JAGUNG (Heterodera zeae) DI INDONESIA Yuliantoro Baliadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/292

Abstract

Identification of nematode species is the first step in the resolution of major nematological problems. Accurate identification of maize cyst nematode species is essential for more detailed research, particularly for it control measure. Although morphological observations by using the key characters of each nematode are suitable for identification purposes, the cyst nematodes could not be clearly identified based on it due to the wide variations. For the identification of the cyst nematode species, key characters relating to body surface, head, mouth, esophagus, tail as well as habitat and feeding habits was used in the present examination. Procedures for the permanent and temporary preparations of nematodes were used. The nematode specimens were observed under the microscope to confirm the key characters. Results of measurements and descriptions revealed that the cyst nematode collected from maize roots in Madura island have the same ambifenestrate as that of Heterodera zeae. The underbridge is short and thin and there is two layer of bullae arrangement in vulval cone, the first layer with four finger-like bullae located immediately below the underbridge and the second layer very randomly located below the first one. These characteristics are of H. zeae. These complete of morphological identifications are a new report of H. zeae in Indonesia.
DEVELOPMENT AND PATHOGENICITY OF INFECTIVE JUVENILES ORIGINATING VIA ENDOTOKIA MATRICIDA IN AXENIC STEINERNEMATID NEMATODES Yuliantoro Baliadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/293

Abstract

Development and insecticidal activity of axenic infective juveniles (IJs) originating from endotokia matricida in maternal bodies of Steinernema glaseri and S. carpocapsae were investigated with a comparison to IJs developed in monoxenic culture. In comparison with the monoxenic steinernematids, the axenic ones grew slower and produced fewer IJs when they were cultured in a sterile chicken liver extract medium supplement with an autoclaved nematode infected Galleria mellonella larva. The phenomena of endotokia matricida, an intra-uterine development of hatched juvenile, occurred in an axenic culture as did the monoxenic ones. Although it occurred faster in monoxenic culture, the ratio of females bearing endotokia matricida was more numerous in axenic ones. These axenic females also produced IJs through the endotokia matricida phenomenon. Compared to the normal IJs develop in monoxenic culture, the IJs originated via endotokia matricida of axenic nematodes showed lower insecticidal activity and it could not reproduce in G. mellonella cadaver.
EKSPRESI SPERMATOGENESIS TIKUS PUTIH SETELAH INDUKSI DENGAN PROTEIN INSULIN LIKE GROWTH FACTOR–I COMPLEX PLASMA SEMINALIS KAMBING Suherni Susilowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/294

Abstract

The objective of this research was to analyzed the expression of spermatogenesis on white rat after inducted by Insulin Like Growth Factor – I (IGF-I) Complex protein from goat seminal plasm. This research consisted of two phase. The fi rst phase was explorative laboratory experiment concerned with identification, isolation and spesifi cation IGF-I Complex by using gel Native Polyacrylamid gel electroforesis, electroelution and Western Blot. Result of gel Native PAGE indicated that of goat seminal plasm with Comassie Blue Stain consisted of seven bands. Result of Western Blot indicated that molecule of IGF-I Complex of goat seminal plasm to bound specific with anti IGF-I Complex at protein ribbon. The second phase concerned with expretion of spermatogenesis on white rat after induction with isolat of IGF-I Complex protein. Result of the expression spermatogenesis on white rat after induction IGF-I Complex to show the signifi cant different between three groups (p < 0.05). From result above can be concluded that IGF-I Complex protein can increase the total of spermatocyte I and spermatocyte II.
KEANEKARAGAMAN JENIS ANGGREK PULAU WAWONII Diah Sulistiarini
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/295

Abstract

An exploration and inventory of orchid plant was carried out in Wawonii Island, South-east Celebes. The aim of the study was to know about orchid diversity in the area, because no fi eld survey conducted since 1940. For that reason all orchid species fi nding were collected as life collection for planted in the Bogor Botanical Garden and dried collection for stored in the Herbarium Bogoriense. Ninety one orchid species were recorded and 36 species of them were new record of Celebes. Out of 1 species Trichoglottis javanica J. J. Sm. was recognized as endemic of Java. Five species orchid were endemic in Celebes, there are Bulbophyllum balapiuense J.J.Sm., Hyppeophyllum celebicum Schltr., Oberonia exaltata Schltr., Thrixspermum celebicum Schltr. and Malaxis lombasangensis (J.J.Sm.) S. Thomas Schut. & de Vogel.
NERACA EKOLOGI PENAMBANGAN TIMAH DI PULAU BANGKA Studi Kasus Pengalihan Fungsi Lahan di Ekosistem Darat Eddy Nurtjahya; Fournita Agustina; Wike Ayu Eka Putri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/296

Abstract

Unequal understanding among tin mining stake holders on trade regulation often triggered social conflict and therefore it was suggested to build political will especially among national, provincial, and regencial/municipal government levels. One important effort to bring out political will is presenting an independent and scientific study on comparision between socio-economic and environmental impact of tin mining. Purposive sampling was conducted to inconventional miners at reserved forest at Lubuk Kelik hamlet, pepper farmers at Silip village, and rubber farmers at Bencah village. Tin mining changed soil properties and microclimate, reduced vegetation composition and structure, arbuscular mycorrhizal fungi, phosphate solubilizing microorganism, ants, and Collembola population. Land conversion increased and contributed up to 95% income. The welfare improvement, however, was apparent as the recovery cost was neglected by inconventional tin miners, and only pepper land conversion showed benefit. Inconventional tin mining regulation needs improvement in order to increase land use efficiency and recovery cost. Understanding on ecological balance of more people and for more sustainable social welfare should be improved as well.
MENINGKATKAN PRODUKSI FLAVAN-3-OL MELALUI KALUS Camellia sinensis L. DENGAN ELISATOR CU2+ Sutini B; Tatik W; Wahyu W; Sutiman B. Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/297

Abstract

Flavan-3-ol is a secondary metabolite in tea plant that is used as anti obesity agent. The difficulty in obtaining Flavan-3-ol out of tea plant is due to dependency on season, need for large land, need for very intensive care and relatively low production. Therefore flavan 3-ol production needs tobe developed in vitro culture technique. This technique can cope with the handicapes above. It can effectively control production and requires less space. The purpose of this research was to enhance fl avan 3-ol production by modifyingmedium and precursor appropriately. The steps of this process were: (1) Callus induction through cultivating the tea shoot explants on medium fi lled with some growth hormone, (2) fl avan-3-ol induction on callus culture using Cu 2+ elicitor. (3) Observation over callus growth, (4) Observation over qualitative characterizes of flavan 3-ol. The result of the research using Cu 2+ elicitor shows that the production of flavan-3-ol increases by 12.5%.
PREFERENSI DAN KERUSAKAN TUMBUHAN KOLEKSI KEBUN RAYA CIBODAS OLEH BENALU Scurrula oortiana (Korth.) Dans. Sunaryo Sunaryo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/298

Abstract

The mistletoe of Scurrula oortiana (Korth.) Dans. (Loranthaceae) growing on several collection plants at Cibodas Botanical Garden. The mistletoe parasite 48 species of plants collection, what come within in 24 tribe. Plants collection which attacked as the highest is group of Ficus type (Moraceae). While attacked by individual in number at most is Callistemon citrinus (Myrtaceae). The mistletoe has caused the damage at part of branch. Infected and uninfected branches of host species were observed. The result showed that the mistletoe causes degradation of the distal part of the infected branches. Damage value of branch which parasite of mean more than 50%.

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