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Contact Name
Sutiman Bambang Sumitro
Contact Email
berkalahayati@yahoo.com
Phone
+62341570631
Journal Mail Official
wulidanisa@berkalahayati.org
Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia
Location
Unknown,
Unknown
INDONESIA
Berkala Penelitian Hayati
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Articles 507 Documents
DIVERSITAS GASTROPODA DI SUNGAI SUKAMADE, TAMAN NASIONAL MERU BETIRI, JAWA TIMUR Putut R. Purnama; Nimas W. Nastiti; Melia E. Agustin; Moch. Affandi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/299

Abstract

Sukamade River is one of the main rivers in Meru Betiri National Park, especially at Sarongan Resort. Macrofauna benthic community in this river are in the protected areas that allegedly still original, and has not got the impact of human activity. Information about the diversity of gastropods class in Sukamade River in limited encourage to do research on the diversity of gastropods in this place. This research was conducted to determine the diversity of gastropods in Sukamade River. Gastropods samples taken at five stations those are in estuary, middle, and part of the river that closer to upstream river using surber-net and hand net. Surber-net used to take samples in watersheds zones and riparian zones using hand net. Samples were obtained, inserted the container that contains the fixative solution that formed by formalin 6% for preservation. Samples that have been preserved, and then sorted, identified and counted the number of individuals of each species, and data analysis. Data analysis, among others, to calculate the diversity index, evenness index, and dominance index in the Laboratory of Ecology Department of Biology, Airlangga University. From the analysis, obtained by the number of species found are 20 species that range from 8–14 species per station, the diversity index ranged from 1.860 to 0.795, evenness index ranged from 0.383 to 0.723 and the dominance index ranged from 0.223 to 0.659. Can be concluded that the diversity of gastropods in Sukamade River is at low-medium category.
PERANAN POHON INDUK DAN PENGARUH PEMUPUKAN DAUN TERHADAP POLA PERTUMBUHAN SEMAI CENDANA (Santalum album L.) Albert H. Wawo; Fauzia Syarif; Budiardjo Budiardjo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/300

Abstract

Sandalwood (Santalum album L.) is one of the economic plants in Indonesia that is threatened rare, because over exploitation and is not cultivated intensively yet by local people in province of Nusa Tenggara Timur (NTT). Therefore need some obviously activities in conservation. One of those activities is producing sandalwood seedlings for cultivation. Sandalwood seeds were collected from mother seed trees. In recent years was found two mother seed trees that grew in 2 locations in Belu District of NTT province were; Tialai location, subdistrict of Tasifeto Timur and Alas locatian, subdistrict of Kobalima. Sandalwood seeds from two mother seed trees was mentioned have been germinated to produced seedling. One of some activities in maintenance of seedling is fertilizing. In 2006 at Kebun Benih Kian Rai Ikun, Belu have been carried out the study of foliar fertilizing on sandalwood seedling from two mother seed trees was mentioned above Three levels dose of Bayfolan had been sprayed every 2 weeks during 10 weeks. The study used Factorial Completely Randomized Design with 3 replications. The results of this study as follow. The growth of sandalwood seedling was infl uenced by mother seed trees whereas foliar fertilizing with Bayfolan was not give significant effect on growth of sandalwood seedling. Seedling growth from both mother seed trees follow the logistic growth model with determination coeffi cient (R2) = > 0.90.
EFFECTS OF BIOFERTILIZER CONTAINING MICROBIAL OF N-FIXER, P SOLUBILIZER AND PLANT GROWTH FACTOR PRODUCER ON CABBAGE (BRASSICA OLERACEAE VAR. CAPITATA) GROWTH AND SOIL ENZYMATIC ACTIVITIES: A GREENHOUSE TRIAL Sarjiya Antonius; Dwi Agustiyani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/301

Abstract

Biofertilizer has been identified as an alternative to chemical fertilizer to increase soil fertility and crop production in sustainable farming. The objective of this greenhouse study was to evaluate the effects of four different concentrations of biofertilizers containing Pseudomonas sp., Bacillus sp. and Streptomyces sp. on soil properties and to evaluate the growth of Brassica oleraceae var. capitata. The application treatments included control (no fertilizer) and four concentration of diluted biofertilizer per pot (20 ml, 40 ml, 60 ml and 80 ml). The application of biofertilizer containing benefi cial bacteria signifi cantly increased the growth of B. oleraceae. The use of biofertilizer resulted higher biomass weight and length as well as root length. This greenhouse study also indicated that different amount of biofertilizer application had almost similar effects. Microbial inoculum not only increased plant harvest, but also improved soil properties, such as number of microorganisms, respiration and urease activities.
PERUBAHAN AKTIVITAS ENZIM LIPASE DARI BUAH KELAPA SELAMA PERTUNASAN Moh. Su'i; Harijono; Yunianta; Aulani'am
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/302

Abstract

This research learned about lipases activity from coconut during germination. Coconut was growth for 0, 15, 30, 45 and 60 days in the darkplace and rate temperature. Shoot, houstorium, radicless and endosperm were taken and then they were measured weigh and long. Lipases was extracted from each of them and measured volume and activity. The results of research showd that highest lipases activity was in shoot that were grew for 45 days with enzyme activity was 0,060 unit/mg protein.
MORFOGENESIS PADA DAUN, TANGKAI DAUN, DAN RUAS BATANG KENTANG HITAM (Solenostemon rotundifolius (POIR) JK MORTON) SECARA IN VITRO Aryani Leksonowati; Witjaksono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/303

Abstract

Solenostemon rotundifolius (Poir) JK Morton is a minor crop that produce carbohydrate and therefore can be utilized for food diversifi cation and help food security program. This crop is not cultivated intensively but it is planted as a side crop during the dry season. Low yield could be responsible for its unattractiveness for farmer to farm it intensively. Improvement of varieties is needed but it is hindered by the low genetic variability due to lack of sexual reproduction. Manipulation at cell level and biotechnology could be an alternative for improving this crop. The availability of a reliable protocol(s) for plant regeneration is needed. This study is intended to develop reliable regeneration protocols using inocula derived from shoot culture on MS basal medium supplemented with various level of BA and NAA. The results show that inocula of leaf, petiole, and stem internodes respond classically to the varying concentration of sitokinin BA and auxin NAA. The optimum production of shoot that refl ect the best shoot organogenic response were achieved by leaves inoculums grew on medium supplemented with 5 mg/l BA and 0.1 mg/l NAA. This protocol serves as a protocol for further somatic cell genetic experiments to increase genetic variability of this crop.
BIOPRODUKSI FLOROGLUSINOL OLEH JAMUR ENDOFIT COELOMYCETES AFAS-F3 YANG DIISOLASI DARI TUMBUHAN Archangelesia flava L. MERR. Yuliasri Jamal; Praptiwi; Ahmad Fathoni; Andria Agusta
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/304

Abstract

Isolation and identification of secondary metabolites of endophytic fungus associated with the plant of Archangelisia flava Merr. and antibacterial activity test were done in this study. Extraction of biomass of endophytic fungus culture and its secondary metabolites isolation were done in ethyl acetate and methanol solvent then followed by thin layer and colomn chromatography. The fractions from colomn chromatography were tested for antibacterial activity against Escherichia coli by paper disc method. The result showed that the extract composed of 9 fractions. Fraction 4th and 8th had growth inhibition zone, 8 mm and 10 mm respectively, against E.coli at the concentration of 100 mg/ml. Isolation and purifi cation process through several chromatography techniques yielded one major metabolites wich was identifi ed as phloroglucinol by co-chromatography technique on a GC-MS machine. Phloroglucinol can produce 10 mm clear zone on a disc antibacterial assay against E. coli.
KAJIAN PRA-KLINIS PEMBERIAN ORAL BUBUK KERING-BEKU SUSU KEDELAI YANG DIFERMENTASI OLEH Lactobacillus plantarum AP1 DAN Sphingobacterium SP. TB17 PADA TIKUS Achmad Dinoto; Rita Dwi Rahayu; Sri Purwaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/305

Abstract

To investigate the effects of oral administration of freeze-dried soymilks fermented by Lactobacillus plantarum AP1 and Sphingobacterium sp. TB17, in preclinical study groups of male Sprague Dawley rats were fed for 3 weeks with four different test diets: basal diet (CD), basal diet supplemented with 3% (w/w feed) freeze-dried unfermented soymilk (CN), basal diet supplemented with 3% (w/w feed) freeze-dried soymilk fermented by L. plantarum AP1 (CL), and basal diet supplemented with 3% (w/w feed) freeze-dried soymilk fermented by Sphingobacterium sp. TB17 (CK). Our result showed a reduction of serum cholesterol level up to 15% in rats CB after three weeks consuming soymilk fermented by L. plantarum AP1. This yield was relatively higher than that of rats CK consuming soymilk fermented by Sphingobacterium sp. TB17 to reduce serum cholesterol only up to 5%. Although unfermented soymilk (CN) also showed the hypocholesterolomic effect to rats, the reduction of cholesterol serum level was lower than that of rat CL. There were no signifi cant increased in blood glucose and body weight of rats as impact of freeze-dried fermented soymilk. Interestingly, although feed intake tends to increase during fermented soymilk consumption, the body weight of rats did not increase. In general, this study indicated that L. plantarum AP1 soymilk is a promising beneficial food product for the health.
KOMPOSISI KIMIA DAN AKTIVITAS ANTIBAKTERI MINYAK ATSIRI Piper gibbilimbum C. DC.: PIPERACEAE Praptiwi; Yuliasri Jamal; Ahmad Fathoni; Ary P. Keim
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/306

Abstract

Distillation of Piper gibilimbum leaves by water distillation yielded 0.85% light yellow essential oil. Result of GC-MS analyses showed that P. gibilimbum essential oil contained 72 chemical compounds with major components 3-carene (40.64%), β -pinene (8.47%), cineol (6.19%), α-copaene (5.47%) and α-guaiene (9.30%). Antibacterial test with disc paper method indicated that the essential oil at 50% concentration inhibited the growth of Staphylococcus aureus, Bacillus subtilis, Streptococcus mutans, Streptococus viridans, Escherisia coli and Shigella flexneri.
PEMEKATAN EKSOPOLISAKARIDA (EPS) DARI KULTUR BAKTERI USUS DALAM BIOMASSA SAGU (Metroxylon SP.) MELALUI MODUL MEMBRAN ULTRAFILTRASI CROSS-FLOW (UFCF) Agustine Susilowati; Aspiyanto; Achmad Dinoto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/307

Abstract

Cross-flow ultrafiltration (UF) membrane is a main separation and/or concentration techniques influenced by operation condition (flow rate, temperature, pressure, time) and molecular weight (MW). The goal of this experiment was to find out the effect of concentration time of Lactobacillus sp. FU 0811 and Enterobacter sp. FU 0813 cultures in biomass of sago (Metroxylon sp.) on UF membrane performance (flux value and rejection), and retentate/concentrate composition as carrier agent of oral insuline activated compounds. Concentration process was performed through cross-flow UF membrane module at pump motor frequency of 20 Hz (flow rate of 7.5 L/minute), room temperature and operation pressure of 5 bar for each 0, 15, 30 and 45 minutes. The result of experiment showed that retentate/concentrate had better composition and total microbes count than that permeate. Based on EPS as reduction sugar, time needed to concentrate Lactobacillus sp. FU 0811 culture as retentate/concentrate was reached at 30 minutes, with reduction sugar, total solids, total acids concentrations, and total microbes count of 4.2 mg/mL, 0.2443% and 0.013%, and 4.61 log CFU/mL. While, permeate flux value was 43.66 L/m2.hour, and rejections of reduction sugar, total solids, total microbes and total acids on membrane were 85.7–90.6%, 96.6–99.1%, 100% dan 12.9–40.2%. Whereas, EPS as reduction sugar, time needed to concentrate Enterobacter sp. FU 0813 culture as retentate/concentrate was reached at 30 minutes, with reduction sugar, total solids, total acids concentrations, and total microbes count of 0.18 mg/mL, 0.0901% and 0.013%, and 4.806 log CFU/mL. While, permeate flux value was 40.83 L/m2.hour, and rejections of reduction sugar, total solids, total microbes and total acids on membrane were 0–68.75%, 81.4–86.8%, 100%, dan 17.6 – 30.3%. Identification of retentate/concentrate from Lactobacillus sp. FU 0811 culture showed that its presence of EPS compounds as Dextran (MW 10,500 Dalton, Da.), Maltohexaose (MW 342.3 Da.) and Lactose (MW 990.86 Da.)
KEANEKARAGAMAN BAKTERI PENGHASIL EKSOPOLISAKARIDA ASAL SALURAN CERNA MANUSIA Achmad Dinoto; Sugiyono Saputra; Agustinus Joko Nugroho; Rita Dwi Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/308

Abstract

Exopolysaccharide (EPS) is a polysaccharide that is excreted by cell as metabolites. Bacterial EPS has been widely used in food and drug industries. In order to find natural products for foods and drugs, the study was conducted to know the diversity of EPS-producing bacteria isolated from human gastrointestinal tracts. As much of 30 bacterial strains were collected from two healthy people and seventeen of collected isolates were investigated for the capability of producing EPS. As the results of identification by partial 16S ribosomal DNA analysis, those seventeen EPS-producing bacterial strains were closely related with already known bacteria (range between 86 to 100% similarity) and could be classified into seven genus, i.e.: Lactobacillus spp. (9 strains), Enterobacter sp. (2 strains), Escherichia sp. (2 strains), Klebsiella sp. (1 strain), Cronobacter sp. (1 strain), Staphylococcus sp. (1 strain), and Alteromonas sp. (1 strain). In addition, member of Lactobacillus group which have closest relationship with species L. plantarum (7 strains) dan L. fermentum (2 strains) were known to dominate the collection of culturable EPS-producing bacteria from human gastrointestinal tracts.

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