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Sutiman Bambang Sumitro
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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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PENGARUH PUPUK ORGANIK HAYATI YANG MENGANDUNG MIKROBA BERMANFAAT TERHADAP PERTUMBUHAN DAN HASIL PANEN TANAMAN SEMANGKA SERTA SIFAT BIOKIMIA TANAHNYA PADA PERCOBAAN LAPANGAN DI MALINAU-KALIMANTAN TIMUR Sarjiya Antonius; Dwi Agustiyani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/309

Abstract

In field experiments during dry season 2010, the effects of biofertilization on growth of fruit yield Semangka (Citrullus lanatus) and of biochemical properties of soil were investigated. The experimental design was Randomly Block Design (RBD) with four treatments and five replications. The treatments were biofertilizer only; chemical fertilizer only; biofertilizer plus a half dose of chemical fertilizer, and control. The application of biofertilizer which was a mixture of Pseudomonas sp., Bacillus sp. and Streptomyces sp and chemical fertilizers (only 50% of the recommended dosage of NPK) increased vegetative growth (plant height, number of branches) compared to chemical fertilizer treatments only. An addition of biofertilizer with the chemical fertilizer also increased the biochemical properties as well as the yield of C. lanatus compared to other treatments. The possible role of biofertilizer on improving soil enzymatic activities, vegetative growths and yield will be discussed more detail in the paper.
RESPONS EMBRIOGENESIS MIKROSPORA TANAMAN TEBU (Saccharum spp.) PADA SUHU DAN LAMA INKUBASI CABANG MALAI DI DALAM MEDIUM B Suaib Suaib; W. Mangoendidjojo; Mirzawan PDN; A. Indrianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/310

Abstract

Microspore embryogenesis under different temperature and time duration pretreatments of spike segments of Saccharum spp., clone PS862 has been studied in in-vitro laboratory of Faculty of Biology Gadjah Mada University, Yogyakarta. Combination pretreatments of a 4 and 34° C of temperatures, and 0, 2, 4, and 7 days duration of incubation of spike segments in the B medium were studied in this observation. A B5 medium of Gamborg supplemented with 2 mgl-1 2.4-D or 2 mg.l-1 NAA was also studied on the formation of proembryos and embryos under 25°C temperature in the dark for at least 30 days. Results of the research showed that the percentage of viable microspores was decreased as simultaneously prolonged of the time of duration in the B medium either under low temperature or under high temperature pretreatments tested. Incubation of spike segments under low temperature after 4 days was decreased the percentage of viable microspores over 44%. In contrast, under high temperature pretreatment the percentage of viable microspores was decreased over 54%. The higher percentage (12.5%) of binucleate microspores was achieved after 7 days incubation of spike segments in the B medium under the low temperature compared to the high temperature (7.14%). Besides, the higher percentage (87.5%) of simmetrical binucleate microspores as proembryo and embryo precursor was obtained after 2 days incubation in the B medium under the low temperature compared to the high temperature (79.0%). Finally, only B5 medium supplemented with 2 mgl-1 of NAA was yielded the proembryos and embryos after 30 days of incubation.
PENGARUH BEBERAPA FITOHORMON PADA PEMBENTUKAN MUTAN BARLEY (Hordeum Vulgare) DENGAN FENOTIP BIJI ABNORMAL Ira N. Djajanegara
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/311

Abstract

Seg8 is the shrunken endosperm mutant occured naturally in barley (Hordeum vulgare). This recessive mutant does not express xenia which indicates that the endosperm phenotypes depend on the genotype of the diploid maternal parents. This mutant provides an opportunity to analyze factors produced by the female parent that affect the seed development process. One of the factors affecting seed development and growth is phytohormones. In vitro spike culture system adopted from wheat spike culture system was used to investigate the involvement of phytohormones in the grain filling process. Phytohormones were applied during 15 days period of culture. Phytohormones concentrations used were as follows abscisic acid 10–4 M, 5x10–5 M, 10–6M, 5x10–7M and 10–7M. Gibberelic acid concentrations were 5x10–3 M, 5x10–4 M, 5x10–5 M, 2.5x10–5M, 10-5M and 2x10–6M. Cytokinin concentrations were 2x10–6 M, 2x10–7 M and 2x10–8 M. In this experiment, 2 days before anthesis is the best stage to start the spike culture period and 15 days period of culture is sufficient to observe the grain filling process and emergence of the mutant phenotype. The phytohormones treatments as well as their respective concentrations were not able to recover the normal phenotype. Abscisic acid treatment at 5x10-7 M were able to increase the mutant grain dry weight significantly compared to untreated culture but the normal phenotype was not recovered. This indicates that lack of phytohormones was not the maternal factor affecting the seed development process in this particular mutant.
PENGARUH STRESOR FISIK TERHADAP DISTRIBUSI SERT DAN INDEKS APOPTOSIS NEURON HIPOKAMPUS, SERTA DISTRIBUSI TNF-α GASTER TIKUS, DENGAN MEDIASI KORTISOL DAN IL–6 Suparno Suparno
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/312

Abstract

Generally, distres which followed by psychiatric disorders still more medicinal practice controversial especially physically stressor roles on holistic health condition. Therefore, researches still more needed to explain and clarify the infl uences and the role of physically stressors on psychiatric disorders. This study used an psychoneuroimmunologic paradigm, which used posttest-only true experiment design for the research. About 24 female Rattus norvegicus strain Wistar had been used, they were devided into six groups. To investigate functional changes, we had been used hippocampus SERT distribution changes as mirror of central serotonergic malfunction changes. While, to investigate structural changes we had been inspected apoptosis index of hippocampus CA3 regions (hippocampus slicing). We also took the blood from the rats heart to examinate IL–6 and cortisol level, and gastric tissues for TNF-α level examination. Physical stressors lead to arise functional and structural changes include hippocampus SERT distribution, increases hippocampus apoptotic index, and increases TNF-α level, that fi nally arise the distress. These results of statistical analyses demonstrate that physical stressors tend to be mediated by serotonergic neurotransmission pathway. This study also support assumptions that psychological stressors caused and or related with gastrical changes.
DESCRIPTION OF THE MALE OF Pseudomacrochiron Parvum (A. SCOTT, 1909) (COPEPODA, POECILOSTOMATOIDA) WITH REMARKS ON THE FEMALE Mulyadi Mulyadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/313

Abstract

The male of Pseudomacrochiron parvum (A. Scott, 1909), which is unknown so far, is described and fi gured on specimens collected off Labuan, Sunda Strait (06º10’S 105º45’E), westward of the type locality in Indonesian waters, and expand the description of the female.
PENGUJIAN IN VITRO XILOOLIGOSAKARIDA SEBAGAI KANDIDAT PREBIOTIK Asnia Zainuddin; Eddy Bagus Wasito; Ni Nyoman Tri Puspaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/314

Abstract

The objectives of this study were to fi nd the in-vitro effect of xyloolygosaccharide on the cell count of Lactobacillus casei Shirota strain, to identify the effect of xyloolygosaccharide on the production of lactic, acetic, propionic and butyric acid (short chain fatty acid = SCFA), and to prove the effect of xyloolygosaccharide on the change of pH value.This was a laboratory experimental study using complete randomized design with 4 treatments, i.e, MRS broth media added xyloolygosaccharide with concentrations of 0% (control), 1%, 3%, and 5%, which were then inoculated with Lactobacillus casei Shirota strain and incubated for 6,12, 18, and 24 hours. The Lactobacillus casei Shirota strain cell count was counted using Drop plate. Data obtained were analyzed statistically using two way ANOVA with signifi cance level of 5%. The type and level of organic acids, i.e., lactic, acetic, propionic, and butyric acids (SCFA), formed in incubation time of 12 hours, were measured using gas chromatography and the change of pH value during incubation time was measured using pH paper. Results showed that xyloolygosaccharide addition MRS Broth media provided highly signifi cant interaction effect on the cell count of Lactobacillus casei Shirota strain (p<0.05). The result of gas chromatography showed that the addition of xyloolygosaccharide in MRS Broth media could increase Lactobacillus casei Shirota strain metabolism activity that produced lactic, acetic, propionic, and butyric acid. The reduction of pH value showed that the lowest pH value of 3.8 after the addition of 5% xyloolygosaccharide with incubation time of 24 hours. In conclusion, the addition of xyloolygosaccharide with concentrations of 1%, 3%, and 5% in MRS Broth media with incubation periods of 6, 12, 18, and 24 hours can increase Lactobacillus casei Shirota strain cell count, increase the metabolism activity of Lactobacillus casei Shirota strain, capable in producing SCFA in incubation time of 12 hours, and results in the reduction of pH value.
KUALITAS UDANG YANG DIJUAL DI PASAR JAKARTA SELATAN DARI ASPEK MIKROBIOLOGI Harsojo Harsojo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/315

Abstract

This study was to know the quality of shrimp sold at some market in south Jakarta. Parameters measured were total amount of aerobic bacteria, total amount of coliform, Escherichia coli, Staphylococcus, Salmonella and pH. Result of the research shows that no Salmonella detected in all samples observed. However, the amount of aerobic bacteria were found in the range from 81.0 × 10² to 59.0 × 105 cfu/g, while coliform bacteria from 20.0 × 10² to 79.0 × 10³ cfu/g. Total amount of E. coli found in the range 0 to 26.0 ×10³ cfu/g, while Staphylococcus bacteria were found in the range 0 to 26.0 × 104 cfu/g. The pH of shrimp samples were found in the range from 6.60 to 7.24. The total shrimp samples have exceeded allowable limit according to Indonesian National Standard were 85.7%.
KEANEKARAGAMAN JENIS BENALU PARASIT PADA TANAMAN KOLEKSI DI KEBUN RAYA EKA KARYA, BALI Tahan Uji; Sunaryo Sunaryo; Erlin Rachman
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/316

Abstract

Benalu is one of the parasitic plants which have ataccked many collection plants species in Eka Karya Botanical Garden, Bali. Exploration and collection of these parasitic plants in this area are conducted. Four parasitic plants species, i.e. Dendrophthoe pentandra, Helixanthera cylindrica, Scurrula atropurpurea, and S. parasitica are recorded and they attack 32 collection plants species in Eka Karya Botanical Garden. Dendrophthoe pentandra is reported as the highest population species to parasiting collection plants species. While the Myrtaceae family and Syzygium genera are also reported as the highest parasited species.
KARAKTERISASI HIDROLISAT PROTEIN KEDELAI HASIL HIDROLISIS MENGGUNAKAN PROTEASE DARI TANAMAN BIDURI (Calotropis gigantea) Yuli Witono; Aulanni’am Aulanni’am; Achmad Subagio; Simon Bambang Widjanarko
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/317

Abstract

Properties of soy protein hydrolysate produced by protease from biduri plant were studied. The soy protein hydrolysate had different properties due to various concentrations and hydrolysis times of protease from biduri. Enzymatic hydrolysis of the soy protein decreased significantly in the TBA value. This process increased the soluble protein content and promoted the Maillard reaction, resulting in a more brown color. Moreover, the soy protein hydrolysate had a higher value of ‘umami’ taste by organoleptic evaluation.
KONSTRUKSI VEKTOR BINER UNTUK EKSPRESI GEN dip22 (YANG DIISOLASI DARI TEBU VARIETAS M 442-51) PADA TANAMAN Wiwit Budi Widyasari; Sony Suhandono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/318

Abstract

Sugarcane is the principle plant for producing sugar in Indonesia. Water supply is one key element in the agronomy of sugarcane. Sugarcane is a high biomass crop which requires large amounts of water. Low yields of sugar observed in water stressed plants indicate that sugarcane is very sensititive to drought. A number of genes that respond to drought, salt, and cold stress at the trasnscriptional level have been reported. dip22 (drought inducible protein) protein isolated from drought resistance variety M 442-51 was predicted to be a protein regulator to water stress in sugarcane. Increasing of tolerance to water stress by over expression of dip22 genes in high yield sugarcane variety hopefully will maintain sugar production. The goal of this research was to construct a binary vector for dip22 gene expression in plant. dip22 gene from mutated PCR was cloned to pGEM®–T Easy and transformed to Escherichia coli strain DH5a. And then, these gene was isolated again from pGEM®–T Easy-dip22 (pGdip) plasmid using restriction enzymes NcoIand PmlI. pCAMBIA 1303 plasmid is an expression vector which has the constitutive promoter CaMV35S. Recombinant plasmid was transformed to Escherichia coli strain DH5a for plasmid propagation through DNA replication. Recombinant plasmid was isolated, and digested with NcoI and PmlI to examine the presence of dip22 gene in the pCAMBIA 1303 plasmid. The recombinant plasmid was transformed to A. tumefaciens strain LBA 4404. Plasmid isolated from A. tumefaciens was digested with Bst XI and Bst EII to examine the similarity between pCAMBIA 1303-dip22 (pCdip) from Escherichia coli and A. tumefaciens. The result by electrophoresis showed that both plasmids had the same size after digested. It was concluded that the transformed A. tumefaciens strain LBA 4404 bacteria has pCAMBIA 1303-dip22 (pCdip) plasmid indeed. Therefore, this construct of dip22 gene in binary vector can be used for improving drought tolerance in plant.

Page 26 of 51 | Total Record : 507

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