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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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VAKSIN INAKTIF Klebsiella pneumoniae DENGAN PERLAKUAN SINAR GAMMA DAN PEMANASAN SUHU 65° C I. Djajanegara; I. Sugoro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/269

Abstract

Klebsiella pneumoniae is one of coliform bacteria which cause human dan mammalian diseases. The bacteria dominate in dairy cow milk which has suffered from mastitis and has resistent on antibiotic. Vaccination is one of aims to prevent the diseases. Nuclear technique could be used to have a vaccine candidate. This research was conducted to get the influence of inactivated K. pneumoniae by gamma irradiated and heat inactivated as vaccine candidate on mice. The treatment were positive control (infected by K. pneumoniae), Negative control (injection by physiologies NaCl solution), 800 Gy (infected by K. pneumoniae has inactivated with 800 Gy), 1000 Gy (infected by K. pneumoniae has inactivated with 1000 Gy), 30’ (infected by K. pneumonia has inactivated with heat 65° C for 30’), and 45’ (infected by K. pneumoniae has inactivated with heat 65° C for 30’). The parameters were physical condition, weight, organ, total number of red, white blood cells, and intraperitoneal macrophage. The results showed that irradiated vaccines were better than heat. % mortality of positive control was 100% but the treatments were 0%. The physic condition of mice was normal for irradiated treatment, but not for negative control and heat treatment. The weight gain of mice after vaccination and challenge test were decreased and back normal after 2 days. The organ condition of mice was normal after vaccination and challenge test. The total number of red and white blood cells showed the same pattern for all treatment after vaccination and challenge test, but the total of intraperitoneal macrophage was increased after 4 hours vaccination. Based on the results showed that irradiated vaccine has potential to develop as vaccine candidate.
STUDI KEAMANAN PANGAN PADA TAHU PUTIH YANG BEREDAR DI PASAR SIDOARJO (KAJIAN DARI KANDUNGAN FORMALIN) Restu Tjiptaningdyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/270

Abstract

A lot of substance circulating in the market used preservative as a mean to lengthen a period to keeping. One of them is formalin, its very dangerous poisonous chemicals for health. The aim of this research was to identify the existence of preservative formalin at white tahu in Sidoarjo markets. This was a discriptif research. Sample intake used stratified random sampling method. As basis for stratify is market of place, are traditional and modern market. Identification of formalin content used FMR (formalin mean reagent), with color different as basis criterion. The result showed that 65.90% white tahu circulate in Sidoarjo markets contained the formalin, and 34.10% of white tahu did not contain formalin.
Analisis Pestisida Karbaril dengan metode KLT- Densitometri dalam matriks kedelai dan validasi metodenya Sugijanto; Prihatin; Noor Erma Sugijanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/271

Abstract

A simple and rapid densitometric method has been developed for determination of low levels of carbaryl in soy bean matrix samples. Sample carbaryl in the soybean matrix was extracted with acetonitril, chloroform and NaCl 0.2 % solution, and the resulting chloroform layer was separated, added sodium sulphate anhydrous to get free of water than evaporated. After dissolution in methanol, the solutions were spotted on silica gel GF254 plates which were then eluted with toluene: ethyl acetate = 3:1 (v/v). Quantitative evaluation was performed by measuring the absorbance reflectance of the analyte spots at λ 280 nm. This densitometric method is selective, precise, accurate and can be used for the determination carbaryl in soy bean matrix.
Ekspresi Level Gen mRNA Protein Ekstraseluler Otak Embrio Mencit Black-6 UK-12 Akibat Induksi 2-Methoxyethanol : Analisis secara Real Time RT-PCR Yulia Irnidayanti; Win Darmanto; Agus Abadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/272

Abstract

The aim of this research was to investigate impact of 2-methoxyethanol, a major industrial chemical, and its individual metabolites on the expression DNA of the embryonic brain development of black-6 mice. The expression levels mRNA protein of GAPDH, Fibronectin, tenascin, vimentin, Neurofilamen, NCam between brain embrio treatment with 2-ME at gestation day 12 and Embryo control were achieved. The Electroforesis DNA on brain Embryonic day 12 showed that there were expression of GAPDH (447bp), Fibronectin (462bp), NCAM (293 bp), Tenascin (416bp), Vimentin (327), Neurofilamen high (301bp), Neurofilamen medium (289bp), Neurofilamen low (398bp). This Data not showed. The expression of level of mRNA for protein Vimentin at embryonic brain treatment at GD-12 is 487 copies, meanwhile on the embryonic brain control is 209 copies. This expression is tendency very higher than control. Another level of mRNA for protein fibronectin, NCAM, Tenascin, Neurofilament were tendency not difference between embryonic brain treatments and control. Intermediate filaments, vimentin, is found in specific cell types in the developing and adult central nervous systems (CNS), particularly astrocytes. Recently, found that vimentin immunoreactivities were increased in astrocytes and/or macrophages in the spinal cords of rats with autoimmune inflammation). So that The higher level mRNA for protein vimentin caused by effect 2-methoxyethanol. Vimentin contribute to the repair of brain through the migration of activated cells and increased level vimentin at embryionic brain treatment with 2-ME.
PEMBUATAN MINYAK KELAPA SECARA ENZIMATIS DENGAN MEMANFAATKAN KULIT BUAH DAN BIJI PEPAYA SERTA ANALISIS SIFAT FISIKOKIMIANYA Nuzul Wahyuning Diyah; Purwanto; Yunita Susanti; Yuliana Kristiani Dewi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/273

Abstract

Enzymatic process using papain is an efficient and less expensive method for producing coconut oil. This research was conducted to produce coconut oil which meet quality requirement from Cocos Nucifera L using ripe fruit peel and seed of papaya (Carica Papaya L) as enzyme sources. The coconut oil was prepared by mixing of coconut milk and juice of papaya ripe fruit peel and/or seed; and then the oils produced and incubated for 24 hours. The physicochemical properties of the coconut oil were determined including specific gravity, water content, contaminant, Iodine number, saponification number, peroxyde number, acid number, and pelican oil. The yields of coconut oil produced by enzymatic method using those two papain sources were significantly higher than coconut oil produced by vaporization/heating method. The physicochemical properties of the coconut oil in accordance with Indonesian National Standard (SNI), but the coconut oil which was produced enzymatically using juice of papaya ripe fruit peel and seed had higher quality than those produced by heating method. Based on the physicochemical properties of the coconut oil, it concluded that ripe fruit peel of papaya was better than papaya seed as an enzyme source.
Keanekaragaman Anggrek Tanah di Kawasan Hutan Lindung Lemor, Lombok Timur Nusa Tenggara Barat Inggit Puji Astuti; I Dewa Putu Darma
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/274

Abstract

Lemor protected forest is one area of lowland tropical rain forest in Lombok island. Based on the results of vegetation analysis, in this area saves many species of tree stands, shrub, vines, and herbaceous plants which make up the ground floor of the forest. One of the plants which makes up the ground floor of the forest is a kind of ground orchids. These research was aimed to investigate the diversity of any varieties in Lemor protected area as there has no information yet about the diversity of ground orchids stored in this area. The inventory to identify the diversity of ground orchids in that area was done exploratively by investigating the entire area, notes, and make specimen from that species as the materials identification. Identification was done by reffering to herbarium specimens saved in Bogoriense Herbarium, and life plant in Bogor Botanical Gardens, as well as aby literatures related to orchids. Some types of orchids there are genus Corymborkis (1 species), genus Nervillia (3 species), and genus Tropidia (2 species).
PENGARUH MUSIM TERHADAP KERAGAMAN DAN KELIMPAHAN KOPEPODA (KRUSTASEA) DI PERAIRAN MANGROVE ESTUARI T. N. UJUNG KULON, BANTEN Mulyadi; Conni M. Sidabalok
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/275

Abstract

An observation on Copepoda diversity and abundance in three mangrove estuaries in Ujung Kulon National Park, Banten was carried out in June and November 2008. The objective of this observation was to investigate the relationship between diversity and abundance of copepods with some environmental factors which affected the distribution in dry and rainy seasons. A total of 28 species from 17 genera and 14 families of copepods were recorded. The highest density of the copepod community was recorded in June (dry season) while the lowest density was in November (rainy season). The diversity, abundance and distribution of copepods were found to be different in the three sites.
STRUKTUR ANATOMI DAN AKTIVITAS ANTIOKSIDAN BULBUS BAWANG DAYAK (Eleutherine americana MERR.) DARI DAERAH KALIMANTAN SELATAN Evi Mintowati Kuntorini; Maria Dewi Astuti; L. Hartanto Nugroho
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/276

Abstract

The aim of this research were to study the characterization of the microscopic anatomy and testing the antioxidant activity of bawang dayak bulb from several regions in South Kalimantan. Bawang dayak plant samples taken from four (4) regency in South Kalimantan. Bulb anatomical structure was observed by the paraffin method and test preparations of antioxidant activity by DPPH method. IC50 values were calculated based on the formula of the regression equation.The bulb anatomical structures has an epidermis tissue of both surfaces, there is parenchymal tissue. Transport tissue were located in rows with collateral type, there are starch grains in parenchyma cells, and the presence of stiloid crystals between cells parenkim. Extract ethanol bawang dayak bulb from the four districts in South Kalimantan has antioxidant activity against DPPH radicals. The highest antioxidant activity showed on the sample from location1 Comets Village Banjarbaru Municipality (IC50 = 25.3339 μg/ml) and the lowest showed on the sample from location 2 Sungai Paring Village Banjar District (IC50 = 86.9039 μg/ml). Antioxidant activity of bawang dayak extract 4.5 to 15 times weaker compared to BHT (BHT IC50 = 5.5707 μg/ml).
KARAKTERISASI EKSTRAK KASAR FITASE TERMOFILIK DARI BAKTERI KAWAH IJEN BANYUWANGI, ISOLAT AP-17 Aline Puspita Kusumadjaja; Tutuk Budiati; Sajidan; Ni Nyoman Tri Puspaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/277

Abstract

Crude thermophilic phytase was produced by isolate AP-17 that has been isolated from Ijen Crater Banyuwangi. Based on Gram test, isolate AP-17 was gram positive spore forming rod shape bacteria so that it was identified as Bacillus sp. AP-17. Crude thermophilic phytase isolated from Bacillus sp. AP-17 had the optimum temperature at 75° C with activity of 0.1413 U/ml, and its optimum pH was at pH 6 with activity of 0.0875 U/ml. The enzyme was stable when heated at 75° C for three hours and still had 90% activity when it was exposed at pH 5–8, optimum temperature, for one hour.
POLA DOMINANSI CAPIT PADA Uca spp. (DECAPODA: OCYPODIDAE) Dewi Citra Murniati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/278

Abstract

Uca spp was a unique crab species marked with asymmetry of the cheliped. A major cheliped and a minor cheliped has different function. Major cheliped size is several times of minor cheliped size (cheliped dominance pattern). Each species has different cheliped dominance pattern. This study was done to describe cheliped dominance pattern of three Uca species. Major cheliped length and minor cheliped length of twenty male of U. dussumieri, U. vocans and U. perplexa were examined. Cheliped dominance pattern obtained by compared the major cheliped length to minor cheliped length. The two-tailed single sample t test showed similar cheliped dominance pattern within Uca species (P > 0.001). The Kruskal-Wallis test showed different cheliped dominance pattern among Uca species (P>0.001). The cheliped dominance pattern of U. dussumieri, U. vocans and U. perplexa are 5.09, 4.24 dan 4.75. Size and morphology of manus, cheliped finger and cheliped gap result in total length of cheliped.

Page 22 of 51 | Total Record : 507

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