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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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PERBANDINGAN PERTUMBUHAN DAN PERKEMBANGAN GONAD IKAN MAS (Cyprinus carpio Linn.) DIPLOID DAN TETRAPLOID Akhmad Taufiq Mukti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/319

Abstract

The aim of this study were to know growth and gonadal development of tetraploidy common carp. The method that used in this study was experiment. Treatment that used was tetraploidyzation by heat shock 40°C during 1.5 minutes of common carp eggs to 29 minutes after fertilization. Five replicates were carried out for treatment. Parameters test were relative length growth (h), specific growth rate (SGR) and gonadal development of common carp. Data analysis that used was descriptive analysis. The result of this study indicated that tetraploidization teratment influenced on growth and gonadal development of common carp. The relative length growth of tetraploidy common carp was 5.38 ± 0.12 (30-days) and 19.12 ± 0.00 (110-days), as their specific growth rate was 19.87 ± 0.10% BW/day (30-days) and 8.57 ± 0.00% BW/day (110-days). Tetraploidy common carp have fertile characteristic and show gonadal development that same with diploid common carp relatively.
EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN Edy Setiti Wida Utami; Issirep Soemardi; Taryono Taryono; Endang Semiarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/320

Abstract

Research of the structure and development pattern of somatic embryos from callus of leaf explants moon orchid Phalaenopsis amabilis (L) Bl had been done. One year old of plantlets were used as explants sources. Basal leaf of these explants were cultured in Somatic Embryo Induction Medium (SEIM) e.i.: NP(New Phalaenopsis) medium added with 2 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Then somatic embryos were transferred to EMM (Embryo Maturation Medium) e.i.: NP medium added with 1 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Finally, mature somatic embryo were transferred to NP medium without plant growth regulator as Embryo Germination Medium (EGM). The origin of somatic embryos initially from single cell at the pheriphery of embryogenic callus. These cells then devided in mitotic repeatedly formed globular proembryo, elongation embryo, and completed embryo. The structure and development pattern of somatic embryos as the same as with zygotic embryo.
STUDI PERBANDINGAN METODE TRANSFORMASI DNA MENGGUNAKAN VEKTOR Agrobacterium tumefaciens PADA TANAMAN TEBU (Sacharum hybrid) Sri Setyati; Purnama Oktaviandari; Muhammad Hazmi; Bambang Sugiharto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/321

Abstract

In order to compare transient expression of gus gene driven by CaMV 35S and rice ubiquitin RUBQ2 promoters, a DNA transformation was conducted using embryogenic callus and suspension cultures of sugarcane. The transient gus expression was observed by histochemical staining method. The histochemical observation of GUS activity after co-cultivation showed that RUBQ2 promoter produced high level of clear blue spots both in embryogenic callus and suspension cultures, while the CaMV35S promoter was not detected. The suspension cultures slightly increased transient gus gene expression compared to embryogenic callus. However, the histochemical analysis of regenerated putative transformant plants after 5 successive cycles on the selection medium showed no blue spots of gus gene expression. PCR amplification of DNA for CaMV35 or nptII in putative transformant plants confirmed that there was no integration of the transformed gene in the genome DNA. The results suggested a possibility of somaclonal variation with callus propagation, thus did not produce transformed plants. To avoid the somaclonal variation, the transformation was conducted using in vitro plants and multiple shoots without intervening callus phase. Histochemical observation of infected materials after co-cultivation showed that almost all of the infected materials partially exhibited blue color in the basal region. In case of in vitro plants, they rapidly grow and multiplied in the selection medium, thus the method provided an excellent system for the transformation in sugarcane. The results suggest that in vitro plants as well as multiple shoots need further investigation to be used as target tissues for Agrobacteriummediated transformation in sugarcane.
UJI AKTIVITAS ANTIOKSIDAN EKSTRAK AIR DAN EKSTRAK METANOL BEBERAPA VARIAN BUAH KENITU (Chrysophyllum cainito L.) DARI DAERAH JEMBER Moch. Amrun H.; Umiyah Umiyah; Evi Umayah U
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/322

Abstract

Star apple or Chrysophyllum cainito L., family Sapotaceae; which is indigenous in Central America has been grown locally arround Jember, East Java. Ethnobotanical data exhibits its medicinal properties such as: soothing inflammation in laringitis and pneumonia, treatment for diabetes mellitus and cancer remedy which are related to free radical mechanism. Therefore, it is necessary to determine its free radical scavenger activity.There are three types of Jember’s star apple fruit (local name: kenitu): big size, green color with round shape (kenitu hijau bulat); medium size, green color with oval shape (kenitu hijau lonjong); and small size, red purplish color with round shape (kenitu merah bulat). Previous research shown that both water and methanol extracts of kenitu hijau lonjong has DPPH free radical scavenger activity as its antioxidant capacity. In recent research the DPPH free radical scavenger activity was performed on both water and methanol extract of these three types of kenitu. The results showed that kenitu merah bulat exhibits the highest antioxidant activity of these three types with IC50 = 426.118 and 169.094 ppm for methanol and water extracts sequentially in 60th minutes.
SEMI PURIFIKASI DAN KARAKTERISASI ENZIM PROTEASE Bacillus sp. Elidar Naiola; Nunuk Widhyastuti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/323

Abstract

The aim of the research was to find the partial purified of enzyme protease from Bacillus sp. The crude enzyme of protease was produce in rice brand medium (100 gram of rice brand in a liter tofu liquid waste). The enzyme was semi-purified by the procedure of precipitation using ethanol in different percentages of saturation, gel filtration using Sephadex G 100 and Ion Exchanged Chromatography using DEAE Sephadex A50. Specific activities of the enzyme during purification were 5.71 U/mg (crude enzyme); 6.75 U/mg (ethanol precipitations); 37.16 U/mg (gel filtration) and 43.02 U/mg (Ion Exchanged Chromatography). The optimum temperature for enzyme reaction was 45–50 °C, while the optimum pH was 7.0–8.0. Protease was relatively stable after heating until 37–50 °C for 60 minutes. Metal ions had different effects to the enzyme. CaCl2, FeCl3, MnCl2, ZnCl2 and MgCl2 increased enzyme activity, CdCl2 and HgCl2 gave an inhibitory effect, and another of metal ions had no effects to the enzyme.
PENGARUH MUTASI DENGAN RADIASI SINAR GAMMA (Co60) TERHADAP PRODUKTIVITAS JAMUR TIRAM ABU-ABU (Pleurotus sajur-caju) Ira Djajanegara; Priyo Wahyudi; Donowati Tjokrokusumo; Netty Widyastuti; Harsoyo Harsoyo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/324

Abstract

Irradiation aplied to living organisms may have positive or negative effects on physiological and morphological properties of the organisms. One way to gain genetic variation with better properties than the parental strain is by Gamma (Co 60) radiation application. During this experiment, Gama (Co 60) rays was applied to the grey oyster (Pleurotus sajur-caju) mushroom mycellia during exponential phase. Radiation was applied at 0.75 KGray with dose velocity of 1.149 KGray. Analysis of mushroom productivity performances indicate that diameter of mycellia, fresh weight, dry weight, diameter of fruit body and the amount of fruit body of the mutant and control were not significantly different. However, the isozyme pattern showed a different pattern between the mutant and the control which indicates that mutation process has already occured. These data show that mutation did not affect the productivity of the mushroom. Therefore, mutation may affect the nutritional quality of the mushroom instead. Further experiment to verify this possibility is suggested.
IMMUNOGENESITY OF SPESIFIC PROTEIN MOLECULAR WEIGHT 16 KDa (PS16) LEAF OF SIAM CITRUS INFECTED BY CITRUS VEIN PHLOEM DEGENERATION (CVPD) DISEASE Made Sritamin; Aulanni ‘am Aulanni ‘am; IGP. Wirawan; Liliek Sulistyowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/325

Abstract

Citrus Vein Phloem degeneration (CVPD) is an important citrus disese, which damaged citrus plantation and causing decrease of citrus production. In Indonesia, the CVPD disease caused by Liberobacter asiaticum bactery and the disease spread out by vectir insect Diaphorina citri and using infected bud in wood grafting. In infected citrus plant, two specific protein molecules with molecular weigt 16 kDa and 66 kDa are found. These protein molecules are not found in healthy citrus plant. The immunogenicity of PS16 accumulated on leaf of citrus plant infected by CVPD is known yet. The research material were leaves of citrus plant infected CVPD, leaves of healthy citrus plant and reagent used these research are for isolation of the total protein leaf of citrus plant, SDS-PAGE electroforesis, electroelution of PS16, ELISA Methods, Dot-Blot Method, anti-PS16 as aprimery antibody and secondary antibody is anti-Rabbit IgG Conjugated AP. The result of the research showed that of PS16 accumulated on leaf of citrus plant infected CVPD has immunogenic character. It is indicated by increase of the titer anti-PS16 after first immunization ang 2nd booster by indirect ELISA method and can be used to induce antibody (anti-PS16) and so showed that positive reaction between PS16 with anti-PS16. It is indicated by purples dark blue on cellulose membrane by Dot Blot method.
PROFIL DAN PERSEPSI PARA PEDAGANG BURUNG TERHADAP PERDAGANGAN PERKICI PELANGI (Trichoglossus haematodus) DAN UPAYA PELESTARIANNYA W. Widodo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/326

Abstract

The Rainbow Lorikeet (Trichoglossus haematodus) has known one of the forest commodities non-wood’s, which found relative abundantly on trade. To know the appearance and perception of the bird traders towards their conservation efforts of the Rainbow Lorikeets, a survey was done on July 2004 in the Pramuka and Jatinegara bird markets (Jakarta). Questioner’s method was used in this survey with 30 numbers of systematical questions asked for 61 respondents in those bird markets. The profiles of the traders are shown that 80.3% trade birds only; 55.7% of them were average 31–40 years old; 49.2% be educated junior schools; 54.1% have traded for less 10 years and 62.3% of the traders income less than 500.000 rupiahs per month. By Q-square Test were results that 88.5% of the traders have agreed with the wildlife animal protected of a law, and 62.3% and 67.2% were not the same opinion to the goverment programes, namely: captivity and quota systems, respectively.
KEPADATAN, BIOMASSA, DAN POLA DISTRIBUSI KEONG LOLA (Trochus niloticus) DI PULAU SAPARUA, KABUPATEN MALUKU TENGAH Handy Erwin Pier Leimena; Tati. S. Subahar; Adianto Adianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/327

Abstract

Lola (Trochus niloticus) is one of the largest size sea snail that live in coral reef at Indo Pacific region and Indonesia including Saparua Island. In a few recent years, lola population in Saparua Island tends to decline due to its exploitation for industrial need. This work aimed to estimate the density, biomass, and sistribution pattern of lola snail population in Saparua Island, Central Molluccas. Transects strip method was applied to collect the snail samples. Each transect was 100 meters long and two meters wide, which were divided into ten segments (10 m × 2 m), and laid perpendicular to the coast line started from the lowest tide level to the open sea. This study showed that average density of lola snail in Saparua Island is 620 individu/ha, with total biomass is 4.15 ton/ha. In Nolloth, lola snail have the highest density as well as the biomass, whilst in Ullath it shows the lowest density. The further down from the lowest tide level, the larger its shell diameter, and this relation can be expressed by y = –2.118 + 0.053x (r2 = 0.879). Morisita dispersion index revealed that the distribution pattern of lola snail is clumped.
KARAKTERISASI DAN UJI AKTIVITAS Bacillus spp. SEBAGAI AGENSIA PENGENDALIAN HAYATI PENYAKIT LINCAT PADA TEMBAKAU TEMANGGUNG Triwidodo Arwiyanto; YMS Maryudani; Agus Eko Prasetyo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/328

Abstract

Lincat disease of tobacco causing severe losses of the product. Control of the disease with any available measure unlikely giving enough control. A number of Bacillus spp. isolates could suppressed the growth of pathogen in vitro and suppressed the development of lincat disease in the field. This article report the characteristics of six isolates of Bacillus spp. (Ba-4, Ba-22, Ba-24, Ba-30, Ba-33, dan Ba-41). These isolates proven could suppressed lincat disease in the field. Characterization of the isolates include the morphological, physiological characteristics, and pathogenicity against tobacco plant. The results indicated that the bacterial isolates were belong to the genus Bacillus with the following charactheristics. The bacteria were rod shapes, forming endospore, Gram positive, fermentative, positive reaction in katalase, oksidase, and Voges Proskaeur tests. Negative results were obtained for Methyl Red test, hydrolysis of starch, gelatine, and casein. The present isolates could use citrate and several carbohydrates as carbon sources. Reduce nitrate to nitrite. The isolates could grow in the medium with high osmotic pressure, i.e. could grow in the medium with 7% NaCl. The present isolates grew well in the medium with pH of 4.5–10 and could grow in the temperature range of 10–50 °C. According to pathogenicity test, the present isolates were not belong to the plant pathogenic bacteria. The present isolates could suppressed the growth of Ralstonia solanacearum in vitro, and could reduce the egg number of Meloidogyne incognita. According to the physiological characteristics tested, it seem that isolates of Ba-4, Ba-24, Ba-30, dan Ba-33, and Ba-41 having similar charactheristics with Bacillus cereus. The Ba-22 isolate, however, having similar characteristics with B. licheniformis.

Page 27 of 51 | Total Record : 507

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