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All Journal Jurnal Kimia (Journal of Chemistry) Jurnal Kedokteran Syiah Kuala Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati Jurnal Natural Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika Folia Medica Indonesiana
Eddy Bagus Wasito
Departemen Mikrobiologi Kedokteran, Fakultas Kedokteran, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health

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Mechanism of Corneal Epithelial Cells Death by Infection of pseudomonas Aeruginosathrough Analysis Expression of Caspase-1, TNF, RIPK1, RIPK3, Caspase-3 in Rats Model Ismi Zuhria; Nurwasis; Eddy Bagus Wasito; Winarto; I Ketut Sudiana; Reny I’tishom; Nyilo Purnami
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15802

Abstract

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Perbandingan kecepatan pertumbuhan Escherichia coli non ESBL dengan Escherichia coli ESBL Branandito Putra; Linda Dewanti; Eddy Bagus Wasito
Jurnal Kedokteran Syiah Kuala Vol 20, No 2 (2020): Volume 20 Nomor 2 Agustus 2020
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v20i2.18498

Abstract

Abstrak. Penelitian ini bertujuan untuk mengidentifikasi perbedaan kecepatan pertumbuhan antara kelompok bakteri Escherichia coli NON ESBL dengan kelompok Escherichia coli ESBL. Jenis dan rancangan penelitian ini adalah penelitian eksperimental. Besar sampel sebanyak 13 isolat bakteri Escherihcia coli. Teknik sampling yang digunakan non random sampling. Variabel dalam penelitian ini meliputi Isolat Escherichia coli, media cair, fotometer, suhu inkubasi. Kecepatan pertumbuhan bakteri Escherichia coli NON ESBL, yaitu 2,206 ± 0,789. Sedangkan kecepatan pertumbuhan bakteri Escherichia coli ESBL, yaitu 1,963 ± 0,674. Nilai signifikasi Uji T sampel independen perbandingan kecepatan pertumbuhan bakteri Escherichia coli NON ESBL dengan kecepatan pertumbuhan bakteri Escherichia coli ESBL (p = 0,257) yang artinya tidak ada perbedaan yang signifikan.Hal tersebut terjadi karena jumlah sampel penelitian yang kurang dari jumlah minimal sampel yang diperlukan.Kata kunci: Escherichia coli, ESBL, Pertumbuhan.Abstract. This study aims to identify a difference of growth’s velocity between NON ESBL Escherichia coli with ESBL Escherichia coli. Type and design of this study was experimental. The sample size was 13 isolate of Escherichia coli. Sampling technique used was non random sampling. Variables in this study were Isolate of Escherichia coli, liquid media, photometer, temperature of incubation. The velocity of growth in NON ESBL Escherichia coli, was 2,206 ± 0,789. Meanwhile, The velocity of growth in ESBL Escherichia coli, was 1,963 ± 0,674. Significant value of T independent sample test  comparison of growth velocity between NON ESBL Escherichia coli  with ESBL Escherichia coli (p =  0,257) which means there’s no different significant. .It happens because an amount of total sample less than minimum standards amount of sample. Keywords: Escherichia coli, ESBL, Growth.
SUBKLONING GEN -L-ARABINOFURANOSIDASE (abfA) DALAM VEKTOR EKSPRESI pYES2 I Nengah Wirajana; Eddy Bagus Wasito; H.M. Soekry Erfan Kusuma; Ni Nyoman Tri Puspaningsih
Jurnal Kimia (Journal of Chemistry) Vol. 4, No. 2 Juli 2010
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

A Gene encoding -L-arabinofuranosidase (abfa) that had been cloned into recombinant E. coliDH5a/pTP510 was subcloned into Saccharomyces cerevisiae yeast system. The aim of subcloning of abfA gene intoS. cerevisiae is to express the thermostable -L-arabinofuranosidase (AbfA) enzyme in the host that is often termedas Generally Recognized As Safe (GRAS), so that this enzyme earn the broader application like in food and beverageindustries. The gene of abfA was subcloned by the amplification of Polymerase Chain Reaction (PCR) from plasmidpTP510 templat. A pair of primers, pFSacI-Af (forward) and pXhoI-Af (reverse) from designed this research wasused for the amplifcation. The PCR condition was performed as follows : beginning denaturation at 94oC for 5 min;PCR cycle 30 times that consisted of denaturation (94oC for 1 min), annealing (55oC for 30 s), andpolymerization/extension at 72oC for 2 min; and than final extension at 72oC for 7 min. The abfA gene, resulted wasinserted between GAL1 promoter and CYT1 terminator in the pYES2 expression vector. This ligation product wastransformed into E. coli TOP10 host. Restriction analysis of recombinant plasmid from this construction, thedesignated as plasmid pY-Af, showed the expected size, about 7,4 kb, which was the summation of sizes of parentalplasmid ( 5,9 kb) and fragment of DNA insert (1,5 kb).
Gram Negative Bacteria (Escherichia coli) Win Against Gram Positive Bacteria (Staphylococcus aureus) in The Same Media Neisya Intan Cahyaningtyas Agung Putri; Ramadhani Ramadhani; Eddy Bagus Wasito
Biomolecular and Health Science Journal Vol. 4 No. 2 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i2.30177

Abstract

Introduction: Biodiversity of the microorganism in Indonesia lead to the large amount of patient with infection. Human can get infected in two different place, with different kind of bacteria that cause the infection. This may lead to bacteremia without knowing which bacteria type whose causing it, either the Gram positive or Gram negative bacteria, whereas the treatment of this two types of bacteria are different. The aim of this study is to determine the doubling time of the Gram positive and Gram negative bacteria when they are grown in the same lesion and the kinds of bacteria that we need to eliminate first.Methods: Staphylococcus aureus and Escherichia coli bacteria were used as samples in this study. Bacterial culture in nutrient broth with 0.5 OD turbidity were mixed then incubated in incubator with 35˚C. Every one hour within 24 hour, 0.01 ml of bacterial culture was taken in serial dilutionover time, varying between 106 – 1012, . It was then planted in nutrient agar plate with droplets technique. After it had been incubated for 24 hours, we counted the Colony Forming Unit per ml (CFU/ml) to time, then the doubling time of the bacteria. The result were then compared between the Staphylococcus aureus and Escherichia coli group.Results: Two tailed t-test result of the doubling time between Staphylococcus aureus dan Escherichia coli was < 0,05 (p=0,000) wich means that there is significant difference of the doubling time between Staphylococcus aureus (24,35 ± 2,23 munites), and Escherichia coli (18,37 ± 0,50 minutes). When grown in the same media, Gram positive bacteria (Staphylococcus aureus) had slower doubling time than Gram negative bacteria (Escherichia coli) as much as 1.32 times.Conclusion: In bacteremia with two possible kinds of bacterial suspect, we need to eliminate the Gram negative bacteria first.
PENGUJIAN IN VITRO XILOOLIGOSAKARIDA SEBAGAI KANDIDAT PREBIOTIK Asnia Zainuddin; Eddy Bagus Wasito; Ni Nyoman Tri Puspaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/314

Abstract

The objectives of this study were to fi nd the in-vitro effect of xyloolygosaccharide on the cell count of Lactobacillus casei Shirota strain, to identify the effect of xyloolygosaccharide on the production of lactic, acetic, propionic and butyric acid (short chain fatty acid = SCFA), and to prove the effect of xyloolygosaccharide on the change of pH value.This was a laboratory experimental study using complete randomized design with 4 treatments, i.e, MRS broth media added xyloolygosaccharide with concentrations of 0% (control), 1%, 3%, and 5%, which were then inoculated with Lactobacillus casei Shirota strain and incubated for 6,12, 18, and 24 hours. The Lactobacillus casei Shirota strain cell count was counted using Drop plate. Data obtained were analyzed statistically using two way ANOVA with signifi cance level of 5%. The type and level of organic acids, i.e., lactic, acetic, propionic, and butyric acids (SCFA), formed in incubation time of 12 hours, were measured using gas chromatography and the change of pH value during incubation time was measured using pH paper. Results showed that xyloolygosaccharide addition MRS Broth media provided highly signifi cant interaction effect on the cell count of Lactobacillus casei Shirota strain (p<0.05). The result of gas chromatography showed that the addition of xyloolygosaccharide in MRS Broth media could increase Lactobacillus casei Shirota strain metabolism activity that produced lactic, acetic, propionic, and butyric acid. The reduction of pH value showed that the lowest pH value of 3.8 after the addition of 5% xyloolygosaccharide with incubation time of 24 hours. In conclusion, the addition of xyloolygosaccharide with concentrations of 1%, 3%, and 5% in MRS Broth media with incubation periods of 6, 12, 18, and 24 hours can increase Lactobacillus casei Shirota strain cell count, increase the metabolism activity of Lactobacillus casei Shirota strain, capable in producing SCFA in incubation time of 12 hours, and results in the reduction of pH value.
In Vitro Antibacterial Activity of Averrhoa bilimbi Leaves Ethanol Extract Against Salmonella typhi Margaretha Nathania; Eddy Bagus Wasito; Nurina Hasanatuludhhiyah
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 14 No. 1 (2023): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V14I12023.43-47

Abstract

Highlights:1. Typhoid fever, a major health problem for developing countries, requires alternative therapy to reduce drug resistance.2. A. bilimbi, a native plant of Indonesia, has antibacterial potential, which is effective against S. typhi in vitro. AbstractIntroduction: Some native plants in Indonesia have potential effects on health and medication. This study aimed to determine the potential antibacterial effect of Averrhoa bilimbi (A. bilimbi) leaves ethanol extract against Salmonella typhi (S. typhi) bacteria. Methods: This was an experimental study. The antibacterial potency of A. bilimbi leaves was measured against S. typhi in vitro. Different concentrations of the leaves’ ethanol extract were prepared. The minimum inhibitory concentration (MIC) was evaluated by a macro-dilution method using Mueller Hinton broth. The minimum bactericidal concentration (MBC) value was observed by subculturing the specimen from the previous dilution tube to the nutrient agar.Results: The MIC of A. bilimbi leaves ethanol extract against S. typhi bacteria could not be determined because all the treatment tubes’ colors were dark and turbid. Meanwhile, the MBC value was at 500 mg/ml.Conclusion: A. bilimbi leaves extract had potential bactericidal effects against S. typhi with a MIC value that could not be determined.
HIV Genotype Analysis from HIV Infected Patients in East Java Area Yulia Sari Ismail; Soetjipto Soetjipto; Eddy Bagus Wasito; Nasronudin Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.358 KB)

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia.  This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.
Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain Ratna Kusumawati; Eddy Bagus Wasito; Arthur Pohan Kawilarang; Eko Budi Koendhori
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 7 No 2 (2023): Journal Qanun Medika Vol 07 No 02
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v7i2.18606

Abstract

Mycosis is often misdiagnosed as Tuberculosis (TB) with negative sputum smear results or Tuberculosis recurrent. In the last two-decade, mycosis increases dramatically as HIV and immunocompromised incidence rates increased. Even though, the prevalence data of mycosis in TB patient is less researched, the identification using KOH often give a false negative result. To compare the result of fungal identification using KOH and PAS staining. This study is observational analytical using a cross-sectional design with 29 TB samples that have been diagnosed with Ziehl-Neelsen (ZN) stain positive in RSUD Dr. Soetomo. The sputum of every sample was colored with KOH and PAS and observed by the researcher and microbiology analyst. The results of this study were 22 sputa identified with Candida sp positive and 12 sputa with non-Candida positive using KOH staining. Meanwhile, using PAS staining, there were 25 sputa identified as Candida sp positive and 22 sputa as non-Candida positive. The congruency test between KOH and PAS has a fair result for both identifying Candida sp (κ = 0.298; significance = 0.069) and non-Candida (κ = 0.240; significance = 0.095). The identification of candida and non-candida in TB patients using KOH and PAS has a fair congruency result. The PAS staining has better identification results both in identifying candida and non-candida rather than KOH staining. 
Sepsis caused by Salmonella serovar paratyphi B in immunocompromised patient with kasabach-merritt syndrome in dr. Soetoro general academic hospital Surabaya: a case report Suharyadi Sasmanto; Wasito, Eddy Bagus
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 1 (2023): Available online : June 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i1.30

Abstract

Introduction: Salmonella species are recognized worldwide as a common cause of childhood infections, particularly gastroenteritis, bacteremia, and enteric fever. Some salmonella infection cases, especially those caused by Salmonella paratyphi B, which are rare, are reported to cause sepsis in children and neonates. The treatment of Salmonella paratyphi B-induced sepsis is challenging, particularly in immunocompromised babies as in Kasabach-Merritt Syndrome (KMS). This case report aims to report the case of a 1.7-year-old girl who had Salmonella serovar paratyphi B-induced sepsis and presented with KMS. Case description: A 1.7 years old girl has complaints of fever for 5 days prior to admission to the hospital with a history of a growing haemangioma in the abdominal area since the first 40 days since birth. The patient was diagnosed with KMS. During the period of hospitalization, there were no complaints of fever, therefore, the patient was discharged. The next day, the patient complained of persistent fever and general weakness. Vital signs examination revealed tachycardia and hyperthermia. Laboratory results showed the hemoglobin was 8.7 gr/dL, leucocyte 20.640/uL, neutrophil 65%, lymphocyte 20.5%, platelet 14.000 uL, CRP 14.22 mg/dL. The patient was diagnosed with Kasabach-Merritt Syndrome (KMS) and has been in control as an outpatient and received oral therapy with methylprednisolone, propranolol and tranexamic acid. The patient was readmitted for chemotherapy with bleomycin and vincristine as part of the KMS treatment regimen. Empiric antibiotic (200 mg ampicillin i.v.) also administered as Salmonella sp. Bacteria was found on the blood culture. Conclusion: Salmonella paratyphi B is a rare infection, the symptoms are typically mild. Rapid identification of bacterial infection and antimicrobial susceptibility testing is crucial for effective treatment.
Perbandingan Distribusi Dan Pola Kepekaan Acinetobacter baumannii Terhadap Antimikroba Di RSUD Dr. Soetomo Surabaya Periode Januari–Maret 2015, April–Juni 2015, dan Januari–Maret 2016 Silvia Sutandhio; Agung Dwi Wahyu Widodo; Lindawati Alimsardjono; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 1 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i1.1782

Abstract

Acinetobacter baumannii is an environmental bacteria that can cause opportunistic infections such as ventilator-associated pneumonia, burn wound infection, urinary tract infection, and sepsis in immunocompromised patients. A. baumannii infections are troublesome because the bacteria resistance to many antimicrobial agents. Surveillance of distribution and sensitivity pattern of A. baumannii is best to be done frequently in hospitals.Venous blood as much as 10 milliliters was drawn aseptically and transferred into liquid medium Bactec® to be sent to Clinical Microbiology Laboratory of Dr. Soetomo General and Teaching Hospital. Medium that showed bacterial growth was subcultured on Blood Agar Plate and MacConkey Agar, and incubated for 18-24 hours in aerobic condition. Identification and susceptibility test were done with semi-automated system BD Phoenix, and interpreted based onClinical and Laboratory Standards Institute 2015.A. baumannii collected on trimester January-March 2015 were 30 isolates, with 19 isolates being Multidrug Resistant(MDR) A. baumannii (63%) and 6 isolatesPandrug Resistant(PDR)A. baumannii (20%). Isolates from trimester April-June 2015 were 32 isolates, with 21 isolates being MDR A. baumannii (66%) and 2 isolates PDR A. baumannii (6%). Isolates from trimester January-March 2016 were 40 isolates, with 22 isolates being MDR A. baumannii (55%) and 2 isolates PDR A. baumannii (5%).A. baumannii mostly isolated from Intensive Care Unit, followed by Internal medicine ward, Pediatric ward, Surgery ward and Emergency Department. High sensitivity of A. baumanniion trimester January-March 2016 was to imipenem (50%), meropenem (50%), amikacin (53%), cotrimoxazole (53%), dan cefoperazone-sulbactam (55%).. MDRA. baumannii mostly isolated from ICU, which reflects high burden of antimicrobial use. At this moment, antimicrobial agents that can be used as empirical therapy for A. baumannii infection are imipenem, meropenem, amikacin, cotrimoxazole, and cefoperazone-sulbactam. The entire hospital member must implement the Infection Control and Prevention and Antimicrobial Stewardship Program to preventnosocomial infections byA. baumanniiand the emergence of MDRA. baumannii.
Co-Authors , Ivanna , Kuntaman Achmad Basori, Achmad Agung Dwi Wahyu Widodo Alimsardjono, Lindawati Ardhiya Puspita Asnia Zainuddin Bambang Hermanto Branandito Putra Budi Utomo Budiono Budiono Eko Budi Koendhori, Eko Budi Erwin Astha Triyono H.M. Soekry Erfan Kusuma Hanindita, Meta Herdiana Hardiani, Kartika Hardiono Hardiono Hardiono Hardiono, Hardiono I Nengah Wirajana I'tishom, Reny Ishak Samuel Wuwuti Isnaeni Isnaeni Ivanna Kartuti Debora Kartuti Debora, Kartuti Kawilarang, Arthur Pohan Kazuo Sakka Kimura, Tetsuya Kuntaman Kuntaman Kusuma, Soekry Erfan Linda Dewanti Lindarto, Wira W Margaretha Nathania Maria Inge Lusida Maria Inge Lusida Muhammad Amin Nasronudin Nasronudin Neisya Intan Cahyaningtyas Agung Putri Ni Nyoman Tri Puspaningsih Nisa Amnifolia Niazta Nur Aisiyah Widjaja, Nur Aisiyah Nurina Hasanatuludhhiyah Nurwasis Nurwasis Nyilo Purnami Puspita, Ardhiya Radita Yuniar Arizandy Ramadhani Ramadhani Ratna Kusumawati Roedi Irawan S.Pd. M Kes I Ketut Sudiana . Sakka, Kazuo Silvia Sutandhio Soekry Erfan Kusuma SOETJIPTO . Suharyadi Sasmanto Tetsuya Kimura Triffit Imasari WINARTO Wiwiek Tyasningsih Wuwuti, Ishak Samuel Yulia Sari Ismail Zuhria, Ismi