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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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STRUKTUR MIKROSKOPIS KARTILAGO EPIFISIALIS TIBIA FETUS MENCIT (Mus musculus L.) DARI INDUK DENGAN PERLAKUAN KAFEIN Heri Budi Santoso
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 1 (2006): December 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/401

Abstract

Caffeine affects activity of enzyme of polimerase DNA, induce the mitosis of cells mammal of before replication DNA ended the perfection, and also pursue the activity of enzyme fosfodiesterase is hence anticipated by a potential caffeine generate the developmental defect, for example can pursue the process of ossification endochondralis in growth plate. This present research studied the effect of caffeine gift by oral on pregnant dam during organogenesis to structure of histologi growth plate tibia foetus. Twenty four pregnant mice (6 per group) were treated by gavage with 0 (control), 40, 80, 120 mg/kg b.w caffeine from gestation day 6 to 15. On day 18 of pregnancy, fetuses was removed from dams by caecarean section.. Observation of histological structure of the tibial growth plate preparation by paraffin method (Hematoxylin-Eosin staining). The result showed that caffeine cause slightened proliferative zone, maturation zone, and cartilage calcification zone on the mouse tibial growth plate.
ISOLASI DAN KARAKTERISASI EKSTRAK KASAR ENZIM BROMELIN DARI BATANG NANAS (Ananas comusus L.merr) Nuniek Herdyastuti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 1 (2006): December 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/402

Abstract

Brommelain is an enzyme hydrolyze most soluble protein easily and efficiently. This enzyme is used in many industry like food industry. This research aimed to isolation and characterization crude extract brommelain. This enzyme has been extracted from the stems of pineapples to produce crude extract, precipitated with amonium sulfat, and enzyme activity to decided with Bergmeyer methode. The higher activity was 1,996 U/ml in precipitate 40-60% amonium sulfat. Optimum temperature and pH to decided temperature and pH variation was detected based on enzyme activity. Characterization to indicate that bromelain has an optimum temperature at 55°C, optimum pH of 7, KM = 5.074 mg/ml and Vmax = 0.666 mg/ml.second.
KONSENTRASI LOGAM BERAT PADA MAKROFAUNA BENTIK DI KEPULAUAN KANGEAN, MADURA Aunurohim Aunurohim; Gilles Radenac; Denis Fichet
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 1 (2006): December 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/403

Abstract

Study of bioaccumulation of heavy metal at species of macrofauna benthic in Kangean’s archipelago, Madura have been done at February 2004. This study was conducted in the effort data base compiling bioaccumulation of heavy metal, especially in Indonesia. As one of part of “Mussel Watch” program what have been executed by cleft of world angle. Kangean’s archipelago has been selected because its situation was very cloistered and far from modern industrial activity, and also inexistence of data about heavy metal’s accumulation at macrofauna benthic. Sampling has been done in 2 station around island Arjasa (subdivision Arjasa), precisely in Erreng at 6°53’00” S and 115°14’26’’ E and Bilis-Bilis at 6°50’45’’ S and 115°14’54’’ E. Sampling has been conducted by using method of direct collection, and then treated in laboratory to analysis by AAS Spectra AA 250 plus of method flame. Species of Nassarius globosus, Anadara Scapha (big) and Saccostrea cucullata accumulate highest metal of Cu, Cd and Zn (in successively) 192,53 ± 154.65 μg/g dry weight, 13.30 ± 1.55 μg/g dry weight, and 1816.02 ± 367.28 μg/g dry weight.
THE ROLE OF OXIDATIVE PHOSPHORYLATION IN THE MECHANISM OF HUMAN SPERM MOTILITY REGULATION Sudjarwo Sudjarwo; Aucky Hinting; Noor Cholies Zaini
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 1 (2006): December 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/404

Abstract

Energy synthesis for sperm motility in the mitochondria with enzymatic reactions in nucleus and mitochondria is called Oxidative Phosphorylation (OXPHOS), in which the respiratory process is coordinated in nucleus and mitochondria. Enzymes in OXPHOS process are complex enzymes I, III, IV and V in the mitochondria and complex enzyme II in the nucleus. If there is OXPHOS dysfunction, the mutation of mitochondria DNA (mtDNA) will decrease energy (ATP, Adenosine Triphosphate) production and increase Reactive Oxygen Species (ROS). The characteristics of ROS are oxidant for lipid, protein and DNA, and all these reactions lead to the dysfunction of OXPHOS and, in the spermatozoa, they will decrease motility. The detection of ROS, Malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OH-dG), and sperm motility can use Chemiluminicense method, Spectroflourometric method, High Performance Liquid Chromatography (HPLC) method and Markler method, respectively, as World Health Organization (WHO) Procedures Laboratory. These results indicated there was significant indirect correlation between ROS with sperm motility and direct correlation between ROS with MDA, 8-OH-dG.
ISOLATION AND CULTURE OF Celosia cristata L. CELL SUSPENSION PROTOPLASTS Retno Mastuti; Hiroshi Miyake; Takeshi Taniguchi; Yoji Takeoka
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/405

Abstract

Developmental competence of Celosia cristata L. cell suspension-derived protoplasts was investigated. The protoplasts were isolated from 3- to 9-d old cultures in enzyme solution containing 2% (w/v) Cellulase YC and 0.5% (w/v) Macerozyme R-10 which was dissolved in washing solution (0.4 M mannitol and 10 mM CaCl2) at pH 5.6 for 3 hours. The highest number of viable protoplasts was released from 5-d old culture of a homogenous cell suspension. Subsequently, three kinds of protoplast culture media were simultaneously examined with four kinds of concentration of gelling agent. Culturing the protoplasts on KM8p medium solidified with 1.2% agarose significantly enhanced plating efficiency as well as microcolony formation. Afterwards, the microcalli actively proliferated into friable watery callus when they were subcultured on MS medium supplemented with 0.3 mg/l 2,4-D and 1.0 mg/l kinetin. Although the plant regeneration from the protoplasts-derived calli has not yet been obtained, the reproducible developmental step from protoplasts to callus in this study may facilitate the establishment of somatic hybridization using C. cristata as one parent.
OPTIMASI ANALISIS AMILASE DAN GLUKANASE YANG DIEKSTRAK DARI MISELIUM Pleurotus ostreatus DENGAN ASAM 3,5 DINITROSALISILAT Maman Rahmansyah; I Made Sudiana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/406

Abstract

Enzymatic activities of amylase (1.4-α-D-glucan glucano hydrolase) and glucanase (1.4-β-glucanase) that extracted from Pleurotus ostreatus mycelium determined through the reducing sugar accumulation. The enzymes assayed in amorphous cellulose (carboxymethylcellulose = CMC) and starch substrate, respectively. Complexity reaction of the sugar to DNS (3.5-dinitrosalicylic acid) become sensitive caused of adding up oxidative agent of sodium sulfite and phenol. Variation in substrate concentration, combine with incubation period and its temperature was able to promote optimum activities of amylase enzymes. The best possible activity of amylase was turn out on 25° C temperature incubation along 20 minutes in 5% starch substrate assessment. Incubation period more then 60 minutes on amylase and cellulase caused those activities leaved to 40 and 70% down, correspondingly. Modified DNS fastened on reducing sugar twice effectively compared to unmodified one.
VESICULAR-ARBUSCULAR MYCORRHIZAL STATUS OF PLANT SPECIES IN THE PEAT SWAMP FOREST OF SETIA ALAM JAYA, SEBANGAU, CENTRAL KALIMANTAN Suciatmih Suciatmih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/407

Abstract

In order to describe the vesicular-arbuscular mycorrhizal (VAM) status of plants growing on peat soil, a study was carried out in the peat swamp forest of Setia Alam Jaya in Sebangau, Central Kalimantan. Out of 146 plant root samples belonging to 48 plant species from 25 families examined, all plants colonized by VAM fungi namely 14 (29.2%) high level, 32 (66.7%) medium level, and 2 (4.1%) low level respectively.
CONTROL REGION-mtDNA HETEROGENEITY OF KALIMANTAN FALSE GHARIAL (Tomistoma schlegelii) POPULATION: A PRELIMINARY STUDY Hellen Kurniati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/408

Abstract

The preliminary genetic study on Kalimantan "false gharial" from the wild was reported. Eleven tail scutes were collected from eleven individuals that originally consisting of two individuals from Kapuas River, one individual from Sentarum Lake, Jelai River, Mapam River, Perian Lake, and Lamandau River, two individuals from Barito River and three individuals from Mahakam River. PCR amplifying and sequencing 451 nucleotides in average that can be aligned at the same length of control region mitochondrial DNA. Among 11 individuals found eight polymorphic sites that consisted four haplotypes (A, B, C, and D) respectively, which is haplotype A is dominant. Based on phylogenetic tree that constructed by Tamura-Nei parameter, false gharial population in Kalimantan can be divided into two population groups; there were Central-Eastern Kalimantan population group and Western Kalimantan population group. Based on the hypothesis of landmasses separating between central-eastern Kalimantan and western Kalimantan that known as Schwaner Mountains, the genetic distance D = 1.53% was expected to be equal to 20 million years.
KARAKTERISTIK BIODEGRADASI ALKIL SULFONAT LINEAR OLEH Pseudomonas aeruginosa I Made Sudiana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/409

Abstract

Detergent contained of Linear Alkyl Sulfonate (LAS) is toxic material to human, animal and microorganism. Strain S1 isolated from detergent contaminated soil was able to grow in media with LAS as a sole carbon source. LAS degradation took place under aerobic condition, with μmax of 0.31-h, Ks = 7.75 mg/L, Vmax = 1.04 mg/L.hour-1and Km = 8.119 mg/L. Analyses of 16s rDNA revealed that S1 is belonging to Pseudomonas aeruginosa.
THE EVIDENCE OF BACILLIFORM VIRUS A CAUSATIVE AGENT OF WHITE SPOT SYNDROME OF WHITE SHRIMP Penaeus merguiensis Hari Suprapto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/410

Abstract

The rod-shape virus particles were found in the hyperthrophied nucleus and cytoplasm of diseased white shrimp Penaeus merguiensis naturally infected by White spot syndrome (WSBV). In natural infection cumulative mortality of shrimp were very high, 95% of population were dead in 3-7 days, and 5% of shrimp population survived. The disease was outbreak in intensive culture farms used the closed circulation sea water system. Others crustacean such as crabs and bentatos were not infected by WSBV at time of sampling. The virus particle were rod-shape ranging from 78 ± 10 nm in diameter and 280 ± 10 nm in length. The pathogenic bacteria mainly dominated by genus Vibrio sp were isolated from shrimp.

Page 35 of 51 | Total Record : 507

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