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Sutiman Bambang Sumitro
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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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KONSTRUKSI MUTASI DAERAH RESISTEN RIFAMPIN (GEN rpoB) DARI Mycobacterium leprae PADA PENDERITA LEPRA DI SURABAYA MELALUI ANALISIS GENOM HASIL PCR E Bimo Aksono H
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/381

Abstract

Rifampin is a key component in the chemoterapeutic regimens used to combat both leprosy and tuberculosis. Owing to exquisite rifampin susceptibility of Mycobacterium leprae, this drug is the backbone of the multidrug therapy currently recommended by WHO for the treatment of leprosy. Resistant mutant are known to arise in leprosy patients receiving rifampin (RIF) monotherapy. The aim of this study was to elucidation of the sequence of the M. leprae rpoB gene permitted identification of mutations associated with rifampin resistance of leprosy patients in Surabaya by genome analysis. M. leprae was detected by nested PCR. In brief, PCR was run with the sense primer rpoBF and anti sense primer rpoBR for 45 cycles. Amplified DNA was analyzed by 3% agarose electrophoresis and the 342 base pairs product was visualized by UV fluorescence after staining with ethidium bromide. PCR product will be purified by phenolchloroform methods and then sequencing directly by ABI PRISM 310. After that sequence data from samples will be analyzed by Genetic Mac ver. 8.0, and comparing with reference data from Gen bank. The result show that only six of 10 samples could be analyzed construct of mutations by Genetic Mac ver 8.0. They have construct no mutation or 100% homology with reference (Z14314 or GI:44382).
KONFIRMASI SPESIFITAS GAD65 TERHADAP ANTI-GAD65 PADA TIKUS DM DAN PASIEN DM TIPE 1 Aulanni’am Aulanni’am; Djoko Wahono Soeatmadji; Sutiman B Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/382

Abstract

The use of glutamic acid decarboxylase (GAD65) from bovine brain has been studied to obtain basic knowledge and diagnosis and prediction of Type 1 Diabetes Mellitus (DM) patients. The importance of GAD65 in DM diagnosis based on its patogenesis. One of the autoimmune marker that can be used to detect beta-pancreas destruction in Diabetes Type I is the antibody to glutamic acid decarboxylase (GAD65). Most of the pre-diabetic patients indicate the reactive autoantibody to GAD65. For early detection of anti-GAD65 in the serum of the patient, human recombinat GAD65 has been succeed to be used. However this is not economical, therefore, it is necessary to find the alternative source of cheaper GAD65. The aim of this research is to develop an early detection kit of Type 1 DM based on antibody-GAD65, since the longest patient suffering from DM has higher probability to be complicated, especially for uncured patients. The anti- GAD65 antibodies induced by anti-GAD65 synthetized and labelled by alkaline phosphatase can be used as reagent detection early DM patients. The ten patients of DM as samples (positive of anti-GAD65) and five rats of DM were positive with western blott technique using reagents as result of this research. It can be concluded, GAD65 enzyme isolated from bovine brain induced anti-GAD65 production and have possibilities to be packaged in a diagnostic kit for patient pre DM.
PENGARUH PERASAN UMBI AKAR LOBAK (Rhapanus sativus L) TERHADAP PENINGKATAN KADAR PROLAKTIN DALAM DARAH MENCIT (Mus musculus) BETINA Mas Loegito; Paulus Liben; Thin Soedarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/383

Abstract

The objective of this research was to prove that the administration of radish tuber extract (Rhapanus sativus L) to female mice (Mus musculus) might increase blood prolactin level. This was an experimental research using complete randomized design by involving three treatment groups and three control group, each with five replications. Materials consisted of radish tuber extract and 30 female mice of 8 weeks old divided into 6 groups, i.e., control groups of C1, C2, and C3, and treatment groups of T1, T2, and T3. C1, C2, and C3 groups were given water of 1 × 0.5 cc/day per oral, 2 × 0.5 cc/day, and 3 × 0.5 cc/day respectively. Treatment groups of T1, T2, and T3 were given radish tuber extract of respectively 1 × 0.5 cc/day per oral, 2 × 0.5 cc/day, and 1 × 0.5 cc/day. Treatment was carried out for 21 days. Blood was subsequently taken from those rats for blood prolactin level. Data were analyzed using Anove test. If the results indicated significant difference of 95% (p = 0.05), the analysis was continued with LSD. The conclusions of this experiment is the administration of radish tuber extract per oral may result in the increase of blood prolactin level.
SIFAT KUALITATIF DAN KUANTITATIF KUSKUS DI PULAU MOOR KABUPATEN NABIRE PAPUA Supriyantono Supriyantono; M Jen Wajo AL; Killian SD; Rumetor Rumetor; Abdillah D Sawen
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/384

Abstract

Reserch on cuscus diversity at Moor island was carried out on November to December 2002. The aim of the research was to know qualitative and quntitative traits, feed and mating behaviour, anatomy of digestion tract and anatomy reproduction organ. Method of case study was used in the research. We found two species of cuscus at Moor island which were Phalanger orientalis and Spilocuscus maculatus. Generally, S. maculatus is bigger than P. orientalis. Both of them can be distinguished easily. Digestion tract of S. maculatus is better than P. orientalis. The form of reproduction organ of those cuscus is very specific and no differ each other.
PENGARUH WAKTU INSEMINASI TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA PASCAINSEMINASI PADA KAMBING Indah Norma Triana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/385

Abstract

The purpose of this research was to know the effect of insemination time on motility and viability of spermatozoa in egg yolk tris diluter post insemination of goat. In this research 20 female goats was used and divided into two groups and synchronized with PGF2 alfa. If sign of oestrus appeared, then goats in group I, were inseminated with semen from buck diluted with egg yolk tris at the beginning of oestrus and group II inseminated with semen from buck diluted with egg yolk tris at the mid oestrus. Semen was collected from cervical canal of goats at one, two, 3, 6, or 24 hours after insemination for evaluating its motility and viability. Implication of this research is indicate that artificial insemination can be conducted in the early also the mid oestrus of goat.
HUBUNGAN KADAR MDA SPERMA DENGAN INTEGRITAS MEMBRAN SPERMATOZOA TIKUS (Rattus norvegicus) SETELAH PEMAPARAN 2–METHOXYETHANOL Alfiah Hayati; Soesanto Mangkoewidjojo; Aucky Hinting; Sukarti Moeljopawiro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/386

Abstract

In the body, 2-methoxyethanol compound may be converted to MAA. MAA is a strong oxidant and may cause oxidation stress in spermatozoa. Oxidation stress is a disturbance on phosphorilation that increases ROS concentration, and it produces lipid peroxide in spermatozoa membrane resulted in high MDA concentration. One of indicator of spermatozoa membrane disturbances is a lack of spermatozoa membrane integrity. The main purpose of this research was to determine the relationship between MDA concentration in sperm and membrane integrity of spermatozoa in rats. The animal of treated groups (n = 40) were divide into 8 groups of 5 each. The rats were given subcutaneous injection with 0,2 ml of 200 mg/kg/day for 1 day (P1), 3 days (P2), 6 days/week (P3), and 12 days/two weeks (P4), respectively the control group was injected with physiological saline of the same volume. The concentration of MDA was measured by spectrophotometer and observing membrane integrity used HOS method to watch the spermatozoa response on hypoosmotic condition. The results of the research indicated that 2-ME caused the increasing in sperm MDA concentration and the decrease of spermatozoa membrane integrity. There was negative correlations between MDA concentration and spermatozoa membrane integrity.
PENGARUH ANTIMITOSIS EKSTRAK Achyranthes Aspera Linn PADA PEMBELAHAN SEL EMBRIO (CLEAVAGE) Wurlina Wurlina
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/387

Abstract

To study the antimitotic effect of an extract of the leaf Achyranthes asapera Linn on embryonal cleavage rats (Rattus norwegicus). Rats ova were fertilized in vitro, and thes suitable zygote and embryo were selected to use as samples, which were divided into 5 groups. Each group comprised 80 ova/embryo. Those groups were: group 1. ova for fertilization, group 2. zygote, group 3. 2 cell embryo, group 4. 4 cell embryo and group 5. 8 cell embryo. Each group was divided into 4 treatmens, each of which consisted of 20 ova/ embryos. The treatments were as follows: control treatment receiving TCM 199 media, and treatment 1, 2 and 3 receiving Achyranthes aspera Linn alkaloid of 20, 30, and 40 ppm respectively. Observation to embryonal cleavage and development was out 12 and 24 hours after treatment. Concluded from the results that the administration of Achyranthes aspera Linn as much as 30 ppm in vitro could 1) inhibits fertilization zygote and 2, 4, and 8 cell embryonal cleavage, growth and development 2) induced zygote and embryonal membrane recruitment and blastomere degeneration and 3) inhibits zygote and embryonal mitotic cleavage at metaphase stage.
PENGGUNAAN FORMALIN UNTUK PENGENDALIAN SAPROLEGNIASIS PADA TELUR IKAN NILA MERAH (Oreochromis sp.) Sri Puji Astuti Wahyuningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/388

Abstract

Saprolegniasis was a primary fungal disease that attacks fish eggs and fish. This disease was caused by Saprolegnia sp. The infections of Saprolegnia sp. in fish eggs can be minimized with dip treatment in formalin solution. This esearch was the control of saprolegniasis on red nila eggs (Oreochromis sp.) with formalin. This research used 400 red nila eggs. It was dipped suspension of Saprolegnia sp. (OD550 = 0,5) for 36 jam. The treatment was divided into four groups. Each group used 10 replication and each treatment with 10 eggs. The eggs in group 1 as control were not treated. The egg in-group 2, 3, and 4 were dipped formalin for five minute. The concentration of formalin in-group 2, 3, and 4 were 4 ml/L, 5 ml/L, and 6 ml/L. These data was taken after the treatment and the investigating parameter was the mean percentage of totally eggs that not infected by Saprolegnia sp. These data was analyzed by ANAVA and LSD at α = 5%. The results of this experiment showed that the increasing of formalin concentrations at each treatment caused significantly decreasing at the growth of Saprolegnia sp. on the red nila fish eggs. The control (1) group was not decreasing of the growth of Saprolegnia sp. The growth of Saprolegnia sp. in-group 2 decreased until 82%. The growth of Saprolegnia sp. in-group 3 decreased until 92%. The growth of Saprolegnia sp. in-group 4 decreased until 96%.
MENINGKATNYA HIDROGEN PEROKSIDA PADA VARIAN T16189C mtDNA SEMEN MANUSIA Sudjarwo Sudjarwo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/389

Abstract

Mitochondria are a site of cellular respiration through oxidative phosphorylation enzymatic reaction (OXPHOS), which is producing energy in the form of ATP (Adenosine Triphosphate). If abnormalities occur along cellular respiratory chain, ATP will decrease and Reactive Oxygen Species (ROS), will increase, one of which is hydrogen peroxide. ROS is an oxidation whose targets are lipid, protein and DNA, all of which may result in the decrease of spermatozoa motility. The detection of hydrogen peroxide was conducted by means of chemiluminescence using luminol, while the detection T16189C mtDNA variant was done using PCR-RFLP with restriction enzyme MnLI. In normozoospermia, hydrogen peroxide in 16189T was 4.4 ± 1.8 CPM/106 sp and in 16189C was 6.4 ± 1.8 CPM/106 sp. In asthenozoospermia, hydrogen peroxide in 16189T was 20.3 ± 8.3 CPM/106 sp while in 16189C was 62.5 ± 9.0 CPM/106 sp. Hydrogen peroxide in normozoospermia and asthenozoospermia 16189T and 16189C showed significant difference (p < 0.00; p < 0.01). In normozoospermia and asthenozoospermia, 16189T and 16189C has correlation with the decrease of motile spermatozoa motility (normozoospermia, p = 0.02; p < 0.05; asthenozoospermia p = 0.03; p < 0.05).
ANALISIS ISOENZIM UNTUK MEMPELAJARI VARIASI GENETIK SAPI BALI DI PROVINSI BALI Sri Rahayu; SB Sumitro; T. Susilawati; Soemarno Soemarno
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 1 (2006): December 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/390

Abstract

The aim of this research was to know Bali cattle genetic variation according to the band pattern of isoenzyme. Esteraseand Malate dehydrogenase Isoenzymes of Bali cattle were studied. Using the native-PAGE method, the analysis has been made of the genetic structure and variation of the bali cattle population. Isoenzyme isolated from leucocytes cell using homogenation method by adding Phosphat Buffer Saline (PBS). A hundred sample of Bali cattles were taken in Mambang, Slemadeg and Kuwumkeladi. The result of this research indicate that from the 2 different enzyme, 3 loci were detected, and 1 of them was polymorphic (MDH-2). There was null allele phenomenom in MDH-2 locus. The loci polymorphic proportion of three population were 0,333. Chi-Square analysis of three population were 1.251–1.560. The heterozygosity value of three population (Mambang, Slemadeg and Kuwumkeladi) were 0.098, 0.111 and 0.118, respectively.

Page 33 of 51 | Total Record : 507

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