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Contact Name
Sutiman Bambang Sumitro
Contact Email
berkalahayati@yahoo.com
Phone
+62341570631
Journal Mail Official
wulidanisa@berkalahayati.org
Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia
Location
Unknown,
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INDONESIA
Berkala Penelitian Hayati
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Articles 507 Documents
PENGGUNAAN ZOLETIL DAN KETAMINE UNTUK ANESTESIA PADA FELIDAE I Komang Wiarsa Sardjana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/411

Abstract

Zoletil and ketamine as a non barbiturat anaesthetic can be administered by the intramuscular route in Felidae a specially in the wild animals and pets. Seven Felidaeof the wild animals there were five the Lions (Panthera leo) and two the White tigers (Panthera tigris tigris) of the Surabaya Zoological Garden have of Zoletil with dose 5 mg/kg body weight and seven Cats of Veterinary Hospital of Faculty of Veterinary Medicine of airlangga was used Ketamine as anaesthetic with dose 20 mg/Kg body weight. All the animals have injected Atropin sulfate with dose 0.1 mg/Kg body weight intramuscular as a premedication. The result of this study of Zoletil in Felidae are shown that the animals have not in respiratory depression and during anaesthesia have done the body temperature means about 36.9º C, Pulsus rate is 100.8 times/minutes and Respiration rate is 21.7times/minutes. The studi of Ketamine the data shown during anaesthesia the means of the body temperature of the cats is 38.4º C with pulsus rate is 85.1 times/minutes and respiration rate is 41.1 time/minutes. We have assumed that study of the drug have a great effect of the animals in practice look like in cats or the wild animals for restraint or anaesthesia of short duration.
PENGARUH EDTA DALAM MENGHILANGKAN KADAR KADMIUM YANG TERAKUMULASI DALAM BERBAGAI ORGAN UDANG Macrobrachium sintangense de Man Agoes Soegianto; M. Yusuf Alamudi; Miftakhul Ulum; Eko W. Putro; Nining D. Widyayanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/412

Abstract

The objective of experiment is to know capability of EDTA to reduce the concentration of cadmium, which accumulated in Macrobrachium sintangense organ. Two groups of shrimps were exposed to 30 mg/L and to freshwater (control) during 7 days and continue with EDTA 0.1 and 1 ppm during 7 days. After finish the experiment, gill, hepatopancreas, carapace and abdominal muscle dissected and destructed with concentrated HNO3 to be analyzed the concentration of cadmium which is accumulated in those organs. The result showed that the concentration of cadmium in gill of control shrimp are 1.696 ± 0.3920 mg/Kg, hepatopancreas 1.1810 ± 0.545 mg/Kg, carapac 0.1025 ± 0.1524 mg/Kg, and abdomen 0.201 ± 0.349 mg/Kg. The concentration of cadmium in exposed shrimps which treated with 0.1 ppm of EDTA are 66.4090 ± 3.22 mg/Kg in gill, 26.4791 ± 0.86 mg/Kg in hepatopancreas, 8.8039 ± 0.66 mg/Kg in carapac; and 3.6324 ± 0.13 mg/Kg in abdomen. The concentration of cadmium in exposed shrimps which treated with 1 ppm of EDTA are 34.8809 ± 4.24 mg/Kg in gills, 9.7290 ± 1.03 mg/Kg in hepatopancreas; 4.6574 ± 0.34 mg/Kg in carapac; and 1.8546 ± 0.43 mg/Kg in abdomen. The greater elimination of cadmium occurs at the shrimp, which is treated with 1 ppm of EDTA.
PROFIL HUTAN MANGROVE TAMAN NASIONAL BALURAN JAWA TIMUR (Mangrove Forest Profile of Baluran National Park East Java) Sudarmadji Sudarmadji
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/413

Abstract

Five mangrove profiles at Baluran National Park East Java were studied. The location was Popongan, Batu Sampan, Kelor-Manting, Si Rondo Si Macan, dan Bilik beach. Each profile was found to be different in their distribution and population. They have demonstrated that there is no constant sequence for mangrove of contrasted areas, because some species tend to prefer a more seaward of a more landward site.
AKTIVITAS ANTIMIKROBA EKSTRAK DAUN JAMBU BIJI DARI BEBERAPA KULTIVAR TERHADAP Staphylococcus aureus ATCC 25923 DENGAN "HOLE-PLATE DIFFUSION METHOD" Farida Lanawati Darsono; Stephanie Devi Artemisia
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/414

Abstract

A study has been performed on the antimicrobial activities of “jambu biji” (Psidium guajava Linn) leaves from several cultivars (red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of “jambu biji” are frequently used in traditional medicine as a remedy against diarrhoea. The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of “jambu biji” for each cultivar were obtained by reflux with ethanol 96%. The concentrations of the extracts applied to the holes were 10%, 20%, and 30% w/v, the extracts were reconstituted with tween 80 and ethanol 96%. Based on the result of the study, it can be concluded that the extract of “jambu biji” from each cultivar with the concentration of 10%, 20%, and 30% w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anava Factorial 3×3 and furthery tested for significancy (α = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.
EFEK ANTIINFLAMASI PERASAN KERING BUAH Morinda citrifolia Linn SECARA PER ORAL PADA TIKUS PUTIH Wahjo Dyatmiko; Suprapto Maat; Idha Kusumawati; Gemparing Bayu Wiyoto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/415

Abstract

Research on anti inflammatory activity of the fruit of Morinda citrifolia Linn. Or known as ‘pace’ in Indonesia language has been done by using three methods of assays i.e. anti inflammatory bioactivity assay by measuring the reduction of carrageen-induced oedema in rats; analgesic bioactivity assay using writhing test method on mice; and vascular permeability bioactivity assay on mice induced by acetic acid.Results showed that dried juice of the fruit at the dose 1500 mg/kg body weight decreased the volume of oedema up to 64% and reduced the pain up to 54%. At the dose 2100 mg/kg body weight the dried juice reduced the vascular permeability up to 72%.
KARAKTERISTIK GENETIK PADA FAMILI CERVIDAE (Cervus unicolor, Cervus timorensis, dan Axis kuhlii) BERDASARKAN 12SrRNA mtDNA Wirdateti Wirdateti; Gono Semiadi; Toshinao Okayama
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 2 (2004): June 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/416

Abstract

Genetic analysis from three species of Indonesia Cervidae (sambar deer, Cervus unicolor; rusa deer, Cervus timorensis; and Bawean deer, Axis kuhlii) was conducted to analyze their relationship. Tissues and blood from twelve sambar deer, one rusa deer and three Bawean deer were collected and analyzed for 12SrRNA using Primer forward (L1091) and reverse (H1478). The results indicated the amplication of mtDNA were 389 base nucleotide. There were 22 polimorphic sites, which were dominated by transition and gave 9 haplotypes that were 5 in sambar deer, 1 in rusa deer and 3 in Bawean deer.
ISOLASI DAN ANALISIS GEN YANG RESPONSIF TERHADAP CEKAMAN KEKERINGAN PADA TEBU Wiwit Budi Widyasari; Bambang Sugiharto; Cahya Ismayadi; Kabul Wahjudi; Untung Murdiyatmo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 2 (2004): June 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/417

Abstract

This study was aimed to isolate and identify a gene that was responsive to drought stress in sugar cane variety M442-51, a variety that is widely used in pastures or dry land. Under drought stress, M442-51 showed an enhance expression of several proteins. One of the protein, namely dip22, (drought inducible protein, with a molecular weight of 22KDa) was isolated and analyzed. Amino acid sequence of this protein showed some homology with several stressed related proteins (OsAsr 1 from Oryza sativa leaves, ZM Bss1 of Zea mays leaves, LeAsr 1, LeAsr 2 and LeAsr 3 of Lycopersicum lycopersicon leaves). The 343 bp of dip22-cDNA fragment was obtained using RT PCR from mRNA of sugar cane leaves that has been exposed to drought stress. The results indicated that the gene dip22 plays an important role as a regulatory protein that are usually nested within cytoplasmic matrix or in the nucleus.
PERBANDINGAN PENINGKATAN BERAT BADAN ANAK MENCIT YANG DIINDUKSI OLEH PEMBERIAN INFUS DAUN PEPAYA DAN DAUN KATU IB. Rai Pidada
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/418

Abstract

A comparative study of increasing on body weight of mice ,s offsprings caused by leaf of papaya (Carica pepaya L.) and katu (Sauropus androgynus (L). Merr) were carried out under laboratory conditions. The study was designed using totally 50 mice post partum (PP) which devided into five groups of treatment consistent 10 females mice post partum of each group. The groups were: The control group, group gave orally in 0.5 ml aquadest, 10%/0.5 ml, and 20%/0.5 ml leaf infuse of papaya and 10%/0.5 ml and 20%/0.5 ml leaf infuse of katu respectively. The treatment were started on day 3rd to 16th of lactation period. The sample were observed on day 4th, 6th , 8th, 10th, 12nd, 14th, and 16th of lactation period. The data were analyzed by ANOVA and LSD test (α = 0.05). The result of study showed that the increasing of body weight of offsprings were significantly different amongs of five groups. Between the control groups and katu groups was not occurred significantly different. The conclusion of this study is papaya groups more right than katu groups to induce an increasing on body weight of mice,s offsprings.
PENGARUH INFUS RIMPANG TEMULAWAK (Curcuma xanthorrhiza) TERHADAP KADAR HEMOGLOBIN DAN JUMLAH ERITROSIT TIKUS PUTIH YANG DIBERI LARUTAN TIMBAL NITRAT [(PbNO3)2] Sugiharto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/419

Abstract

The purpose of this experiment was to study the effect of turmeric (Curcuma xanthorrhiza) rhizome infuse to hemoglobin concentration and number of erythrocyte of [(PbNO3)2] treated rats. Pb was presumed to be link at sulphyhydril groups that may caused an inhibition to several enzymatic process, such as d-ALAD and heme sintetase which is caused the inhibits of Hb synthesis and erythropoesis. Twenty female rats was used in this experiment. They were divided into five groups, i.e. (A) control (treated with 1 ml aquadest); (B) treated with 1/2 ml of 12 ppm lead solution and 1/2 ml aquadest; (C) treated with 1/2 ml of 12 ppm lead solution and 1/2 ml of 20% turmeric rhizome infuse; (D) treated with 1/2 ml of 50 ppm lead solution and 1/2 ml aquadest; (E) treated with 1/2 ml of 50 ppm lead solution and 1/2 ml of 20% turmeric rhizome infuse. The treatment was given orally every day (30 days) using a modified syringe. After 30 days of treatment, the blood sample were taken about 2 ml by heart puncture. The Hb concentration was determined using by Cyanmethemoglobin method and number of erythrocyte was counting on Haemocytometer Improved Neubauer. Data were analyzed by Anova and LSD test (α = 0.05). The results of this study shows that 20% of turmeric rhizome infuse statistically has a significant effect (P < 0.05) to increasing of hemoglobin concentration but hasn’t significant to prevent the number of erythrocyte decreasing of [(PbNO3)2] treated.
ULTRA STRUCTURE OF BAUNG (Mystus nemurus) SPERMATOZOA Muchlisin ZA
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 2 (2004): June 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/420

Abstract

Ultra struktur sperma ikan baung (Mystus nemurus) telah diamati dengan menggunakan transmisi dan imbas elektron mikroskop (TEM dan SEM). Hasil penelitian menunjukkan bahwa spermatozoa normal ikan baung terbagi atas tiga bagian yaitu; kepala, leher yang pendek, dan bagian ekor. Kepala sperma ikan baung berbentuk bulat yang diseliputi oleh membran sitoplasma, pada bagian leher terdapat mitokondria, sedangkan ekornya bersifat tunggal dan irisan melintangnya menunjukkan bahwa centriol mikrotubulusnya terbentuk atas “9+2” axoneme.

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