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Media Veteriner
Published by Institut Pertanian Bogor
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Articles 7 Documents
 1 
Search results for , issue "Vol. 3 No. 1 (1996): Media Veteriner" : 7 Documents clear
In Vitro Development Of Holstein And Japanese Black Breeds Embryo A. Boediono; T. Suzuki
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Abstract

Two experiments were conducted with 3,457 oocytes, aspirated from the ovaries of slaughtered Holstein and Japanese Black breeds cows to compare the use of superovulatedcow serum (SCS) and fetal calf serum (FCS) supplementation into medium for in vjtro development (experiment I) and the effect of different source of oocytes (experiment II). Theendpoint was development to cleavage on day 2 and to blastocyst up to day 9 after msermnabon. In experiment I, oocytes were matured and cultured m vltro (after fertilization)in TCM-199 supplemented with 5% SCS or 5% FCS. The cleavage rate of zygotes cultured in medium supplemented with 5% SCS was higher (P
The Evaluation Of Potasium Thiocyanate Extracted Pasteurella multocida Vaccine On Pig E.S. Pribadi; C.S.U. Pramono; M.B.M. Malole
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Abstract

Pasteurella multocida has been isolated from pigs at Kapuk Farm West Jakarta that had been diagnosed pasteurellosis. These isolates were extracted with potassium thiocyanate(KSCN) and has been evaluated as vaccine for pig. Seventeenth 3-4 years-old pigs were used for experiment and they were divided to four groups. i.e (i) control, (ii) vaccination, (iii)vaccination and challenge and (iv) challenge groups. All pigs were fed and receive water ad Irbiturri. The experiment was carried out for 30 days. All experiment groups showed no difference statistically for antibody titer until days-21. At days-23, vaccination groups showed antibody titer higher than non vaccination groups.Challenge groups showed clinical sign i.e. anorexia, abdominal respiration, rhinitis and demonstrated pathological changes i.e. local dermatitis superficialis, limphadenitis, oedem cervicalis ventral, trcheitis, laryngitis, pleuropneumoni, emphysema pulmonum, pleuritis, pericarditis, epicarditis, hipertrophy ventricle dextra, dilatation ventricle sinistra, splenitis, anteritis catarhalis, tephlitis, colitis, cystitis,, myositis necroticans, gastritis catarhalis and gangrenosa, degeneration  parenchymatosa. hidronephrosis, sinusitis catarhalis,pneumoni lobuler and epiglositis. Histopathological changes showed alveoli damaged, germinal ccnters formation in spleen and timpltoglandulae which was more severe in thechallenge groups. P. multocida was isolated from visceral organs that showed pathology and histopathology changes.Mild clinical signs was observed in the vaccination groups. This groups showed similar pathological changes as other groups but therc were no d e m a at cervicalis region. This group showed chronic histopathological changes. No P. nrultocido was isolated from visceral organs. It can be concluded that all vaccinated pigs was protected by vaccine during the 30days experiment.
Conception Rate In Ewes Having Different Number of Ovulating Follicle During The First Estrous Cycle After PGF2, Injection M.Y Sumaryadi; Wasmen Manalu
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Abstract

Forty sis ewes were used to study the effects of number of ovulating follicle on the conception rate during the first estrous cycle after PGF& injection. The experimental ewes were injected twice with PGF& to synchronize estrous cycle and to remove previously existed corpus luteum. The number of ovulating follicle was determined by counting the number of corpus luteum by laparoscopy, 5 days after the estrous cycle. The distribution of ewes having 1. 2. 3, and >3 ovulating follicle was 12, 19, 12, 3, respectively. Conception rate in each group during the first estrous cycle after the last PGF& injection was determined by counting the parturition date. The results of the experiment showed that the hgher the number ofovulating follicle the greater the percentage of ewes being pregnant (33,33, 63,12, 58,33, and 10%, for ewes having 1, 2, 3, and >3, ovulating follicle, respectively). It was suggested that an increasing number of ovulating follicle could be used to improve conception rate in ewes.
Fasciolasis Pada Domba dan Kambing di Rumah Potong Hewan Kotamadya Bogor W. Winarsih; E. Estuningsih; A. Setiyono; E. Harlina
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Abstract

Telah dilakukan penelitian fasciolasis pada domba dan kambing yang dipotong & Rurnah Potong Hewan Kotamadya Bogor pada bulan November sarnpai Desember 1992. Sebanyak 192 ekor domba dan kambing telah diamati terhadap kejadian fasciolasis pada organ hatinya. Metode penelitian yang dilakukan adalah dengan melakukan pemeriksaan berdasarkan skoring derajat kerusakan pada organ hati dengan melihat gambaran makroskoplk dan mikroskopik.Hasil pemeriksaan rnakroskopik diperoleh 14,06% menunjukkan gambaran hati normal dan 85.94% terinfeksi yaitu 76,04% terinfeksi akut dan 9.88% terinfeksi kronis.Sedangkan gambaran mikroskopik organ hati yang terinfeksi secara akut adalah perdarahan. degenerasi sel hati, peradangan dan proliferasi buluh empedu. infiltrasi selradang. dan admya 'globula leucocvte' pada mukosa buluh empedu. Pada infeksi kronis tampak fokus-fokus radang granuloma, mineralisasi dan fibrosis.4
The Study on Aflatoxin Accumulation in Visceral Organs of Chicken Received Corticosteroid and Antibiotic U. Patriana; E.S. Pribadi
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Abstract

Seratus lima puluh ekor ayam petelur jantan digunakan dalam penelitian ini. Ayam-ayam tersebut dibagi ke empat kelompok besar yang terdiri dari Kelompok pertama adalah kelompok yang menerima aflatoksin dan antibiotik (45 ekor). Kelompok kedua adalah kelompok yang menerima aflatoksin dan kartikosteroid (45 ekor), Kelompok ke tiga adalah kelompok yang menerima afklatoksin saja (45 ekor) dan kelompok ke empat adalah kelompok kontrol. Masing-masing kelompok besar dibagi lagi menjadi tiga kelompok kecil dan tiap kelompok yang berbeda memperoleh aflatoksin dengan dosis 150, 250, dan 350 ppb di dalam media tepung beras melalui pakan. Pengamatan dilakukan terhadap bobot organ jeroan, perubahan patologi anotomi dan kandungan aflatoksin dalam masing-masing organ jeroan. Bobot organ kelompok yang menerima aflatoksinlebih berat dibandingkan yang tidak dan kelompok yang menerima obat antibiotika dan kortikosteroid lebih ringan dibandingkan yang tidak. Terjadi perubahan patologi dari organ hati, limpa dan bursa Fabrisius pada semua kelompok yang mendapatkan aflatoksin. Kandunagn aflatoksin di organ hati meningkat dan peningkatan  pada kelompok yang mendapatkan antibiotika dan kortikosteroid lebih rendah dibandingkan kelompok yang hanya menerima aflatoksin. Kandungan aflatoksin di organ limpa dan busra Fabrisius menurun dan penurunan lebih cepat pada kelompok yang mendapatkan antibiotika dan kartikosteroid.
The Effect Of Feeding Concentrate On The Digestibility And N-Balanced Of Java Deer (Cervus timorensis) W.R Farida; Wirdateti M.S.A. Zein
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian konsentrat (makanan penguat) ke dalam ransum terhadap daya cerna dan N-balance pada Rusa Jawa (Cewus tzmorensis). Empat ekor Rusa Jawa betina digunakan dalam penelitian ini. Rancangan penelitian yang digunakan adalah bujur sangkar latin (4x4). Selama penelitian masing-masing rusa ditempatkan di dalam kandang metabolisme. Penelitian dilaksanakan dalam 4 periode, setiap periode terdiri dari 10 hari masa adaptasi pakan dan 6 hari masa pengumpulan feses dan urin. Keempat ekor rusa masing-masing mendapat ransum secara bergantian pada setiap periode. Ransum yang diberikan adalah : Ransum I (kontrol) = 3000 g rumput gajah + 2000 g ketela rambat + 50 g garam dapur + 15 g mineral: Ransum I1 = 3000 g rumput gajah + 1500 g ketela rarnbat + 500 g pelet + 50 g garam dapur + 15 g mineral; Ransum 111 = 3000 g rumput gajah + 1500 g ketela rambat + 250 g bungkil kedele + 250 g dedak + 50 g garam dapur + 15 g mineral; dan Ransum IV = 3000 g rumput gajah + 1250 g ketela rambat + 500 g bungkil kedele + 250 g dedak + 50 g garam dapur + 15 g mineral. Hasil penelitian menunjukkan kecernaan protein dan lemak meningkat sangat nyata (p
The Effect of Stage of Estrous Cycle on The Development of Bovine Embryo Matured and Fertilized in Vitro I. Djuwita; B. Purwantara; Y. Sukra; M. Fahrudin; A. Winarto
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Abstract

The objective ofthis study is to examine the effect of stage of estrous cycle on in vitro development of bovine embryo matured and fertilized in vitro. Cow ovaries were collected from slaughter house and were kept in a physiological solution. Oocytes were aspirated using GI8 neddle connected to 10 mL syringe containing phosphate buffer saline (PBS). After being washed, oocytes were matured in vitro in Tissue Culture Medium (TCM) 199 a physiological in 5% CO2 incubator at 39°C for 20-22 hours. In vitro fertilization was done in BO (Brackett and Oliphant) solution for 8 hrs, using frozen semen. Embryos were further cultured in either TCM-199 supplemented with 5% cow superovulated serum or chemically defined-serum free medium. If ovaries were classified due to their estrous stages, i.e. luteal and follicular, both cleavage (fertilization) and development rates did not show any differences (p < 0,5) in both treatment. The cleavage rate of oocytes collected from the follicular and luteal stages were 75.0% and 75.6%. respectively even though was lower if compared to that withaout classification (83.5 %). The development rate were 20,0% and 23,0%, higher, compare to that without classification, i.e. 13.0%. Nevertheless, if chemically defined-serum free medium was used, the two treatments showed differences (p < 0,5) in both cleavage and development rates. The ratio between luteal and follicular stages were 68,0% : 80,0% and 53,6%: 65,7% for cleavage and development rates, respectively.

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