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Bioscientiae, Jurnal ilmu ilmu Biologi
Published by Universitas Lambung Mangkurat
ISSN : 28081838     EISSN : 28084438     DOI : https://doi.org/10.20527/b.v19i1
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Veterinary
Jurnal Ilmiah BIOSCIENTIAE adalah jurnal ilmiah yang diterbitkan oleh Program Studi Biologi Fakultas MIPA Universitas Lambung Mangkurat untuk mempublikasikan karya ilmiah mahasiswa, dosen, dan peneliti di bidang biologi. Setiap naskah yang diterima redaksi Jurnal Bioscientiae akan ditelaah oleh Mitra bestari dan Anggota Redaksi. Jurnal Bioscientiae menerbitkan paling banyak 2 (dua) review article tiap terbitan. Terbit dua kali setahun, pada bulan Januari dan Juli. Terbit pertama kali tahun 2004.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 154 Documents
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RESPON PUPUK KANDANG AYAM DAN NPK TERHADAP PERTUMBUHAN DAN HASIL TERONG UNGU saijo, saijo saijo
Bioscientiae Vol 22, No 1 (2025): Bioscientiae Volume 22 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v22i1.14615

Abstract

The purpose of the study was to test and analyze the response of chicken manure and NPK 16:16:16 application on the growth and yield of purple eggplant plants in sandy land. The study used a Randomized Bloc Design, consisting of two factors. The first treatment was the provision of 4 levels of chicken manure with doses of 0, 30, 35 and 40 t ha-1 while the second treatment of NPK 16:16:16 consisted of 3 levels, namely doses of 0, 300 and 350 kg ha-1. From the two treatment factors, 12 combinations were obtained. Each was repeated 3 times, so that there were 36 experimental units. The results showed that the response of chicken manure affected all growth variables, both plant height, stem diameter and number of productive branches at a dose of 30 t ha-1. While the dose of 40 t ha-1 affected the yield variable in the form of the number of fruits per plant with the largest number of 8 fruits per plant with the heaviest fruit weight of 223.89 g fruit-1. In addition, the dose response of NPK 16:16:16 also affects the yield variable in the form of the number of fruits per plant at a dose of 350 kg ha-1 with the largest number of fruits being 6 fruits per plant-1.
Emisi Gas CH4 dan CO2 serta Perubahan Kadar C-Organik pada Pengomposan Tandan Kosong Kelapa Sawit dan Rerumputan Menggunakan Bakteri Selulolitik Indigenous Pasang Surut Nur, Hasrul Satria; Mawar, Silvia; Hadi, Abdul
Bioscientiae Vol 22, No 1 (2025): Bioscientiae Volume 22 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v22i1.14558

Abstract

Oil palm empty bunch and grass, are sorts of agricultural waste, that contribute to evolving gas emissions--methane (CH4) and carbon dioxide (CO2).  Therefore, agricultural waste management to compost is one system to minimize the gas emissions in oil palm plantations.  Generally, using bio-decomposer lignocellulolytic bacterium is the way to speed up the composting.  Bacillus brevis BRT-1 and Bacillus megaterium BTB-1, having potency as bio-decomposer agents, are members of cellulolytic bacteria origin of soil swamps tidal.  Adding cellulolytic bacteria indigenous into the composting of oil palm empty bunch and grass are the objectives in our studies to observe, i.e. C-organic changes patterns, methane, and carbon dioxide emissions.  Two inoculants of cellulolytic bacteria comprised of BRT-1 and BTB-1 isolates were applied to the two substrates in composting the agricultural waste by adding cow manure.  The parameters of analysis were observed during twenty days, in addition, C-organic substrates were measured by the Walkley and Black method.  Especially for methane and carbon dioxide were observed by Micro Gas Chromatography, meanwhile, the addition of two inoculants of cellulolytic bacteria had a significant impact on C-organic changes during the composting.  Otherwise, using cellulolytic bacteria in this study showed no significant effect of methane and carbon dioxide.  Moreover, the result is also some of the depicted parameters, particularly of C-organic contain undergone alleviate on oil palm empty bunch substrates of BTB-1 – KS treatments.  Besides that, the C-organic content of grass substrates also decreased in BTB-1 treatments, furthermore, the methane emissions for oil palm empty bunch underwent fewer BRT-1+KS treatments.  On the other sides, the methane emissions increased during observation in grass substrates of BRT-1+KS treatments.  The same cases for the carbon dioxide emissions in oil palm empty bunch substrates are also decreased in BRT-1 treatments.  Meanwhile, the carbon dioxide emission underwent the escalation of grass substrates in BRT-1+KS treatments.  The utilization of cellulolytic bacteria Indigenous has the potential as a bio-decomposer agent in composting especially in accelerating C-organic changes.
PROFIL M. tuberculosis PASIEN TB SENSITIF RIFAMPISIN TERHADAP LAMA WAKTU PENUNDAAN PEMERIKSAAN SPUTUM Sulistiyawati, Indah; Fatimah, Siti; Rahayu, Nur Laila
Bioscientiae Vol 22, No 1 (2025): Bioscientiae Volume 22 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v22i1.14676

Abstract

Tuberculosis (TB) is an infectious disease that is currently still a serious health problem in society, both in terms of mortality rates, morbidity rates, diagnosis and its therapy rates. Sputum quality is an important component in laboratory examinations. The quality of sputum samples can affect the number of M. tuberculosis bacteria. This study aims to determine the differences in the morphological characteristics of M. tuberculosis bacteria with the length of time of delay in sputum sample examination. The study was conducted at the Klinik Utama Kesehatan Paru Masyarakat Purwokerto. The study used a descriptive method with a cross-sectional design. The sample population of this study were all suspected TB patients who underwent Molecular Rapid Technique (MRT) examinations at the Klinik Utama Kesehatan Paru Masyarakat Purwokerto (KKPM) in the January-June 2024. The research sampling technique was purposive sampling that met the inclusion criteria. The results of the study showed that the morphological characteristics of the M. tuberculosis bacteria vary in each sputum sample, based on differences in the quality of the sputum examined with a general description of the morphological characteristics of the bacteria, which have the form of monobacillus, diplobacillus and bacillus, solitary and in colonies, and are a type of Gram-positive bacteria.  Keywords: Mycobacterium tuberculosis, MDR-TB. TCM test, GeneXpert 
ANALISIS MIKROBILOGI COLIFORM PADA PRODUK INDUSTRI RUMAH TANGGA SIOMAY DI KECAMATAN PURWOKERTO SELATAN Sulistiyawati, Indah; Sadiah, Hilmi Halimatus; Rahayu, Nurlaila
Bioscientiae Vol 22, No 1 (2025): Bioscientiae Volume 22 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v22i1.14614

Abstract

Jajanan siomay merupakan salah satu makanan yang banyak diminati oleh masyarakat dan dijual bebas di pasar tradisional. Bahan makanan mudah tercemar oleh golongan bakteri Coliform adalah bahan makanan tradisional dengan pengolahan sederhana. Jajanan siomay rentan terkontaminasi oleh berbagai bakteri patogen akibat kurangnya penerapan higiene dan sanitasi penjamah makanan. Penelitian ini mengidentifikasi keberadaan bakteri Colifom pada produk industri rumah tangga siomay di Kecamatan Purwokerto Selatan. Penelitian ini merupakan penelitian observasional laboratorik dengan teknik standar pemeriksaan mikrobiologi makanan dan minuman menggunakan metode kuantitatif yaitu Most Probable Number (MPN) dan Membran Filter. Teknik pengambilan sampel penelitian adalah purposive random sampling. Variabel yang diamati adalah analisis bakteri Coliform pada siomay, air yang digunakan dalam proses pembuatan siomay dan higiene sanitasi tempat pembuatan siomay dengan melakukan wawancara pengisian kusioner di Kecamatan Purwokerto Selatan. Hasil yang diperoleh menunjukkan 8 dari 12 sampel siomay terkonfirmasi mengalami kontaminasi golongan bakteri Coliform. Pemeriksaan air PDAM yang digunakan dalam pengolahan siomay didapatkan hasil 0/100 mL yaitu memenuhi syarat berdasarkan Peraturan Menteri Kesehatan Nomor 2 Tahun 2023. Higiene sanitasi penjamah makanan menunjukkan bahwa pengetahuan dan sikap penjamah makanan pada pedagang siomay di kelurahan Berkoh dan di kelurahan Purwokerto kidul dari rekapitulasi rata-rata memiliki kategori pengetahuan dan sikap baik.
STRUKTUR KOMUNITAS FITOPLANKTON DI SEKITAR BENDUNG GERAK SERAYU BANYUMAS zaenuri, musyarif
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.15122

Abstract

Serayu Movable Dam (BGS) is a water structure construction that crosses the Serayu River which aims to raise the water level so that it can be used to irrigate agriculture. Changes in air mass will form a community of air organisms. Phytoplankton are air organisms that develop following existing water conditions. The purpose of this study was to examine the diversity of phytoplankton in the waters of the Serayu Movable Dam. This research method uses surveys and stratified random sampling techniques, namely two locations before and two locations after the BGS gate. The main variables observed: Type and number of Phytoplankton. Supporting variables observed include: air and air temperature, Light Penetration, pH, Nitrate and Phosphate. The abundance of phytoplankton in the Serayu Movable Dam is relatively low and falls into the category of mesotrophic waters (moderate fertility). The phytoplankton found came from three main groups: Bacillariophyta, Chlorophyta, and Cyanophyta. Changes in air flow due to blockage affect the number and type of phytoplankton in each location. Air quality is generally still good and below the standard quality limit, but still needs to be maintained through routine management and monitoring. 
IDENTIFIKASI DAN KARAKTERISASI MORFOLOGI TUMBUHAN MAKROFITA DI DANAU TOBA, KECAMATAN PALIPI, KABUPATEN SAMOSIR, PROVINSI SUMATERA UTARA Simbolon, Helda; Sofiyanti, Nery
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18203

Abstract

Macrophytic plants are plants that spend most of their life cycle in water which plays an important role in aquatic ecosystems. Macrophytic plants are pivotal for fish habitat in Lake Toba, Palipi District, such as for shelter, forage and also a place to attach eggs. These plant groups are also important to maintain water quality. The purpose of this study is to provide data on macrophyte plant species and their morphological characteristics in Lake Toba, Palipi District, Samosir Regency, North Sumatra Province. Sampling was carried out using an exploration method. The morphological characters were observed and photographed. The data obtained is then explained in the form of figures and tables, scoring is carried before being analyzed using NTSys pc 2.02 application to obtain the similarity coefficient and construct dendrogram. The results of this study were obtained from 9 types of macrophyte plants (Alternanthera sessilis, Phragmites karka, Ipomoea aquatica, Salvinia minima, Limnocharis flava, Hydrilla verticillata, Vallisneria spiralis, Nymphaea rubra dan Eichorrnia crassipes). The dendrogram is divided into two groups I and II at a coefficient value of 0,28. The similarity coefficient ranges from 0,15 to 0,80.  Dendrogram shows that each group is clustered based on growth type, stems and flowers.
PEMERIKSAAN BAKTERI COLIFORM PADA SAMPEL DEPOT AIR MINUM ISI ULANG (DAMIU) Azizah, Teguh; Sulistiyawati, Indah
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.15249

Abstract

Water is one of the basic human needs, because humans are very dependent on air for both drinking and daily life. Air quality that includes is very important to ensure safe drinking water, but it is also important to consider the broader impacts of air management practices. Refill Drinking Water Depot (DAMIU). This study aims to ensure that drinking water is safe and suitable for consumption. This study was to examine coliform bacteria in drinking water samples from Refill Drinking Water Depot (DAMIU) using the membrane filter method, with 10 samples. This study uses the Membrane filter method, which is a technique used to test water samples to identify the presence of coliform bacteria and Escherichia coli. The results of testing 10 drinking water samples can be concluded that some of the quality of drinking water at the research location is still different and has not fully met the quality standards. The results are not in accordance with the maximum limit of coliform and E. coli in drinking water is 0 CFU / 100 mL in the drinking water quality standards set by the Regulation of the Minister of Health of the Republic of Indonesia (PERMENKES) Number 2 of 2023. The influencing factors are raw materials, length of time the water is stored in the reservoir, handling of the buyer's container, operator cleanliness, and environmental cleanliness around the DAMIU which is less noticed.
PENGARUH PUPUK KANDANG SAPI DAN PEMANGKASAN TERHADAP PERTUMBUHAN DAN HASIL TANAMAN CABAI KERITING DI TANAH GAMBUT Awaliyah, Syarifatul; Saijo, Saijo; Susilo, Djoko Eko Hadi
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18202

Abstract

The objective of this study was to analyze and examine the effects of cattle manure, pruning, and their interaction on the growth and yield of curly chili plants on peat soil. The experimental design used was a factorial Completely Randomized Design (CRD) with two treatment factors and four replications. The first treatment factor was cattle manure: K1 = 20 t ha⁻¹, K2 = 30 t ha⁻¹, and K3 = 40 t ha⁻¹. The second treatment factor was pruning: P0 = no pruning, P1 = apical pruning, and P2 = water shoot pruning. Data were analyzed using the F-test at significance levels of α = 0.05 and 0.01. Mean differences among treatments were tested using the HSD (Honestly Significant Difference) test at the 0.05 significance level.The results showed that the interaction between the two treatment factors had no significant effect. The cattle manure treatment significantly affected the fruit diameter of curly chili, with the most efficient dose being 20 t ha⁻¹. The pruning treatment significantly affected fruit length and fruit weight, with water shoot pruning being the most effective technique.
UJI CEMARAN BAKTERI COLIFORM DAN Escherichia coli PADA AIR BERSIH SEBAGAI INDIKATOR KUALITAS LINGKUNGAN Kafah, Silmi; Sulistiyawati, Indah
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18192

Abstract

Clean water plays a vital role in supporting human health and daily activities. This research aimed to analyze microbiological contamination specifically coliform and Escherichia coli bacteria in clean water as indicators of environmental quality. The study employed the membrane filter method to examine 20 water samples collected from various sources including wells, springs, and PDAM water. Microbiological tests followed the standard procedures set by the Ministry of Health Regulation No. 2 of 2023. In addition to microbiological testing, chemical parameters such as pH and iron content were also analyzed using a pH meter and UV-Vis spectrophotometer. The results indicated that not all samples met the required quality standards. Several samples contained coliform and E. coli levels exceeding the permissible limit of 0 CFU/100 mL, making them unsuitable for consumption. High bacterial levels were linked to poor sanitation practices around the water sources. The study also found that a few samples had elevated pH levels and iron concentrations, which may pose health risks. This research contributes valuable insights into water quality monitoring and underlines the importance of environmental hygiene in ensuring safe water sources.
RANCANG BANGUN POWER SUPPLY ELEKTROFORESIS SEDERHANA: POTENSIAL APLIKASI UNTUK PEMISAHAN DNA/PROTEIN Susilo, Tanto Budi
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18205

Abstract

Agarose gel electrophoresis is a method of separating biomolecules, based on their weight and electrical charge, such as DNA, RNA, and proteins. This method requires a stable and adjustable electrical power supply. However, commercial electrophoresis power supply devices generally have a fairly high price and are not flexible for the needs of small laboratories or practicum activities. This research aims to design and build a simple electrophoresis power supply device that is economical, and portable. The results have been designed and built two units of electrophoresis power supply 60-150 DC, 2-3 A, by engineering a 220 AC power supply, 2-3 A. The engineering process by adding diodes and digital display voltmeter. Voltage and amperage can be adjusted to the needs of gel electrophoresis. This design is compact and relatively low cost to manufacture. This independent power supply can be a viable alternative to support research and learning activities in the field of biotechnology and biochemistry.

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