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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 16 Documents
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Search results for , issue "Vol. 14, No. 1" : 16 Documents clear
DETEKSI CEPAT BAKTERI Escherichia coli DALAM SAMPEL AIR DENGAN METODE POLYMERASE CHAIN REACTION MENGGUNAKAN PRIMER 16E1 DAN 16E2 Radji, Maksum; Puspaningrum, Anglia; Sumiati, Atiek
Makara Journal of Science Vol. 14, No. 1
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Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction Using 16E1 and 16E2 Primers. The detection of Escherichia coli in various water samples by Polymerase Chain Reaction method using 16E1 and 16E2 primers has been carried out. It was found that four out of ten samples were detected Escherichia coli and revealed the presence of the amplified product of the size 584 base pairs. Confirmation test of PCR result was also done using the conventional culturing method. The results of this study showed that the PCR assay was specific and faster than the conventional culturing method for detection of Escherichia coli.
PEMANTAUAN KADAR LOGAM BERAT DALAM AIR LAUT DAN SEDIMEN DI PERAIRAN PULAU BACAN, MALUKU UTARA Marasabessy, M Djen; Edward, Edward; Valentin, Febriana Lisa
Makara Journal of Science Vol. 14, No. 1
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Monitoring on Heavy Metals Content in Sea Water and Sediment in the Waters of Bacan Island, North of Maluku. Measurement on heavy metals content in seawater and sediment in the waters of Bacan Islands, North of Maluku were carried out in September 2005. That heavy metals are Pb, Cd, Cu, Zn, and Ni. Seawater and sediment sample collected from 10 station by purposive sampling, in line with the goal of the research. The results showed that the heavy metals content in seawater still in line with the threshold value (NAB) stated by The Office of State Ministry for Life Environment (KMNLH) but in sediment heavy metals content is high relative, especially Cu and Ni has passed the threshold value for sediment. Beside heavy metal content also measured physical and chemical parameters such as temperature, salinity, total suspended solid, light transmission, dissolved oxygen, acidity, phosphate and nitrate. The result also indicated that parameters still in line with the threshold value stated by KMNLH for marine organism. Based on heavy metals content, the value of seawater quality status included into class A (fine) with score 0. Heavy metal content in sediment is higher than seawater, this condition indicated there are heavy metals accumulation in sediment.
SYNTHESIS AND CHARACTERIZATION OF TiO2(B) NANOTUBES PREPARED BY HYDROTHERMAL METHOD USING [Ti8O12(H2O)24]Cl8.HCl.7H2O AS PRECURSOR Sutrisno, Hari
Makara Journal of Science Vol. 14, No. 1
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PEMBUATAN ARANG AKTIF DARI BATANG JAGUNG MENGGUNAKAN AKTIVATOR ASAM SULFAT DAN PENGGUNAANNYA PADA PENJERAPAN ION TEMBAGA (II) Suhendra, Dedy; Gunawan, Erin Ryantin
Makara Journal of Science Vol. 14, No. 1
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Preparation of Activated Carbon from Maize Stems by Sulfuric Acids Activation and Their Application in Copper (II) Ion Sorption. Activated carbons were prepared from maize (Zea mays L.) stems by sulfuric acids activation or chemical methods. The dry maize stems are usually used as low-value energy resources in many countries, burned in the field, or discarded, which are unfavorable to environment. This motivates the investigation of producing value-added products from the dry maize stems, such as activated carbons, as well as solving some environmental problems. The preparation process consisted of sulfuric acid impregnation at different impregnation ratio followed by carbonization at 250–400 o C for 1–4 h. The results show that the impregnation ratio was 1.25, the optimum activation temperature was 300 o C and the activation time was 1 h. The sorption capacity of the activated carbon was 25.1 mg/g.
PENGARUH KASEIN HIDROLISAT DAN INTENSITAS CAHAYA TERHADAP PRODUKSI BIOMASSA DAN ALKALOID CANTHINONE DI DALAM KULTUR SUSPENSI SEL PASAK BUMI (Eurycoma longifolia Jack) Siregar, Luthfi Aziz Mahmud; Keng, Chan Chan; Lim, Boey Peng
Makara Journal of Science Vol. 14, No. 1
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Effects of Casein Hydrolisate and Light Intensity on Production of Biomass and Canthinone Alkaloid in Cell Suspension Cultures of Pasak Bumi (Eurycoma longifolia Jack). A study on the effect of several concentrations of casein hydrolysate and light intensity to produce biomass and canthinone alkaloid in cell suspension culture of Pasak Bumi (Eurycoma longifolia Jack) was conducted. The research was an experimental study with block random design non factorial. For study of casein hydrolysate was used 8 (eight) of concentration treatments, i.e. 0.00, 0.10, 0.25, 0.50, 0.75, 1.00, 2.00, dan 5.00% (w/v). Beside for study of different light intensity effect was used five of light intensity, i.e. 0, 190, 290, 585, and 1525 lux. The results showed that addition 0.1–2.0% (w/v) of casein hydrolysate into culture medium did not show the effect on production of cell biomass compare with without casein hydrolysate. Total of alkaloid produce increased two fold in the culture medium containing 0.1% casein hydrolysate. The medium with addition 5% casein hydrolysate significantly decrease of increased fresh weight, dry weight and total of alkaloid compare with medium without or additions of casein hydrolysate at 0.1–2.0% concentration. Besides, the modifications on light intensity (0–1525 lux) did not effect the production of cell biomass in culture medium, but the place of E. longifolia Jack cell suspension culture at light of 1525 lux was found have to stimulate increased the total of 9- methoxycanthin-6-one in cells.
EFEK DOPING Ni (II) PADA AKTIFITAS FOTOKATALITIK DARI TiO2 UNTUK INHIBISI BAKTERI PATOGENIK Rilda, Yetria; Dharma, Abdi; Arief, Syukri; Shaleh, Baharuddin
Makara Journal of Science Vol. 14, No. 1
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Influence of Co-Doping of Ni (II) on Photocatalytic Activity of TiO2 for Pathogenic Bacteria Inhibition. Nanoparticle titanium dioxide (TiO2) has most attention in the past decade, since it can be applied as alternative material on sterilization photocatalyst process. This research focused on increasing performance of titania such as structure, particles size and surface area through Ni ion doped on TiO2 surface by sol-gel technique. Product were used to design of a photobioreactor for sterilization process from pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. Product were characterized using Thermogravimetric-Differential Thermal Analysis (TG-DTA), X-ray Diffraction (XRD), Transmition Electron Microscopy (TEM), Scanning Electron Microscopes-Energy Dispersive X-Ray Spectroscopy (SEM-EDX) dan Brunauer-Emmet-Teller (BET). Titanium dioxide with anatase structure have 12.1 nm in particles size and surface area 49.6 m2 / g that have higher inhibition rate to bacteria cell. Photobiocatalytic reaction was carried out in various TiO2-Ni concentration and UV irradiation times. The anti bacteria from TiO2-Ni to all bacteria cell suspension after UV irradiated at λm : 365 nm has good synergistic effect. Effect of mechanical treatment by sonicator showed the increasing inhibition rate around 4% for 120 minute irradiation. Inhibition rate optimization for each bacteria gave different efficiency inhibition to TiO2-Ni concentration 1.5-2.0 g/L. TiO2-Ni powder inhibited growth of Escherichia coli, Staphylococcus aureus around ≥ 95% for 120 minute irradiation, while Bacillus subtilis resistance with inhibition percentage rate only 88.1%.

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