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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 18 Documents
 1   2 
Search results for , issue "Vol 34, No 1 (2016): Juni" : 18 Documents clear
Efek Ekstrak Daun Binahong (Anredera cordifolia (Ten) Steenis) terhadap Profil Darah Merah pada Marmut (Cavia cobaya) Dwi Wijayanti; Enny Tantini Setiatin; Edy Kurnianto
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.975 KB) | DOI: 10.22146/jsv.22818

Abstract

The purpose of this study was to determine the effect of binahong leaves extract (BLE) on Cavia cobaya through the number of erythrocytes, hemoglobin, pack cell volume (PCV) and erythrocyte indices. Complete Random Design (CRD) was used in this study with 4 treatment and 4 replicate, those were T0 (control), T1 (10 mg / head / body weight), T2 (50 mg / head / body weight) and T3 (90 mg / head / weight). The administration of BLE was orally for 10 days pre partum and 10 days postpartum. Blood sampling was done three times, which wasbefore giving BLE , 1 day postpartum and 1 day after the giving of BLE. Data was analyzed using ANOVA and if there was effect of treatment, then continued with Duncan multiple range test. There was not significantly different among treatments for PCV before giving BLE, while the postpartum and post giving BLE showed significantly different (P<0.05). There was not significantl different in the percentage of Hb among treatments for postpartum while in before giving BLE and post giving BLE were showed significantly different (P<0.05). Total erythrocytes was not significantly difference among treatments for before giving BLE while in the postpartum and post giving BLE significantly different (P <0.05). MCHC value was not significantly different between treatments which was before giving BLE and post giving BLE while in the postpartum was showedsignificantly different (P<0.05). MCV and MCH values were not significantly different between treatments before giving BLE while in the postpartum and post giving BLE significantly different (P <0.05). In conclusion, giving BLE at a dose of 50 mg/head/body weight can improve immunity and endurance Cavia cobaya on the treatment group before giving BLE, postpartum and post giving BLE of PCV, Hb, total erythrocytes, MCHC, MCV and MCH.
Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer Yuli Purwandari Kristianingrum; Sitarina Widyarini Sitarina Widyarini; Kurniasih Kurniasih; Bambang Sutrisno Bambang Sutrisno; Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu; Sugiyono Sugiyono
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.772 KB) | DOI: 10.22146/jsv.22819

Abstract

Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected bytrimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brainwere observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell deathbetween control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day aftertreatment, especially in the hippocampus and cortex.
Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer Yuli Purwandari Kristianingrum; Sitarina Widyarini Sitarina Widyarini; Kurniasih Kurniasih; Bambang Sutrisno Bambang Sutrisno; Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu; Sugiyono Sugiyono
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.772 KB) | DOI: 10.22146/jsv.22820

Abstract

Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected bytrimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brainwere observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell deathbetween control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day aftertreatment, especially in the hippocampus and cortex.
Respon Sistem Homeostasis Kalsium Tikus Ovariektomi yang Mengkonsumsi Kombinasi Calcitriol dengan Raloxifene Hartiningsih Hartiningsih; Devita Anggraeni
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.861 KB) | DOI: 10.22146/jsv.22821

Abstract

Normal range of calcium (Ca) level in the blood is maintained by Ca homeostasis in the intestinal, kidney and bone. The objective of this research was to study the response of calcium homeostasis system ovariectomized rats consuming combination of calcitriol and raloxifene by calcium balance study. Twenty five female Wistar rats at 8 weeks of age were divided into five groups of five: normal control (NK), ovariectomy control (OVK), ovariectomy+calcitriol (OVD ), ovariectomy+ raloxifene (OVR), and ovariectomy+calcitriol+ 20raloxifene supplementation (OVDR), of five each. Seven weeks after the surgery, each rats was placed into individual metabolic cages for Ca balance studies (Ca consumption, feces and urine Ca excretion, and intestinalCa absorption). In 5 to 8 days of the balance studies, the remaining food, urine, and feces were collected every day for Ca analyses. The results showed that Ca consumption, feces and urine Ca excretion, and intestinal Ca absorption of OVDR rats were higher than OVK rats. Consumption and feces Ca excretion of OVDR rats were higher than OVK rats which informed the decrease of estrogen hormone. Intestinal Ca absorption of OVDR rats was higher than OVK rats which informed the decrease of parathyroid hormone. Meanwhile, urine Ca excretionof OVDR rats was higher than OVK rats which informed the decrease of parathyroid hormone and estrogen. In conclusions, the response of calcium homeostasis system in ovariectomized rats consuming combination ofcalcitriol and raloxifene for 8 weeks was marked by increasing Ca absorption in intestine and Ca excretion in urine.
Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat Muhammad Dusil Hafid; Hilal Anshary
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2547.633 KB) | DOI: 10.22146/jsv.22822

Abstract

Anisakis (L3-stage) is a parasitic nematode commonly found in marine fish or squid serve as intermediate or paratenic host. The purpose of this research was to identify Anisakis larvae using PCR-RFLP and sequencing methods. A total of 30 individuals of each species: bullet tuna (Auxis rochei) and Indian scad(Decapterus russeli) were examined for presence of Anisakis spp. Fish samples caught by fishermen around Mamuju waters were purchased at a fish market. The fish was necropsied and the organs: liver, intestine wall, heart and muscle were removed and put on separated petri dishes and examined for parasites under a stereo microscope. Anisakis found was cleaned and fixed in 70% alcohol. Initial identification was based on ventriculus shape and presence of mucron to distinguish Anisakis type I and Anisakis type II. All Anisakis found belong to Anisakis type I. Ten Anisakis type I were isolated, cleaned and stored in microfuge tubes containing 70% alcohol. The parasite gDNA was extracted using Wizard genomic DNA extraction and purification kit (Promega).Species of Anisakis was determined by PCR-RFLP at ITS1-5.8S-ITS2 region using two restriction enzymes: Taq I and Hinf I and PCR-sequencing in mtDNA cox2 region. Analysis by PCR-RFLP showed that all Anisakis type Iexamined was in the same pattern as Anisakis typica. Sequencing in mtDNAcox-2 region and the phylogenetic analysis showed that all samples were in the same cluster as A. typica. Based on PCR-RFLP and sequencinganalysis, all Anisakis found in this study belong to A. typica.
Identifikasi Virus Avian Leukosis Sub Grup J pada Peternakan Ayam Petelur Komersial di Kabupaten Tangerang Tahun 2015 Risza Hartawan; Ni Luh Putu Indi Dharmayanti
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.665 KB) | DOI: 10.22146/jsv.22823

Abstract

Avian leukosis virus (ALV) is one of causing agents for neoplastic syndrome in commercial chicken farms. There are six sub groups of ALV in chicken including A, B, C, D and E. However, the sub group J (ALV-J) is the most alarmed and anticipated because of its severe economic repercussion. The objective of this study was to identify the presence of ALV-J in the commercial chicken farms in Tangerang District. Two identification methods were utilized including antigen capture enzyme-linked immunosorbent assay (ac-ELISA) technique and nested reverse transcriptase polymerase chain reaction (nRT-PCR) test. As results, the ac-ELISA test detected that most of 150 serum samples from 5 commercial layer farms were positive for ALV in about 86,67%. On the other hand, none of the 60 cloacal swab and 60 albumen samples was positive for presence ALV. Subsequently, testing of 45 serum samples from 5 farms using nRT-PCR showed that in about 20% of these samples were positive of ALV-J. These evidences indicated the circulation of ALV-J with other sub groups in thecommercial layer farms in Tangerang District.
Daya Hidup Spermatozoa Epididimis Kambing Peranakan Ettawa yang Dipreservasi dengan Pengencer Tris dan Bberbagai Konsentrasi Maltosa Muhammad Rizal; Dwi Sulistiowati; Abrani Sulaiman; Herdis Herdis; Insun Sangadji
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.086 KB) | DOI: 10.22146/jsv.22824

Abstract

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, since the spermatozoa is motile and has ability for fertilizing the oocyte. Theobjective of this research was to examine the effectivity of maltose in maintaining viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3–5oC. Five testis with epididymides of ettawa crossbreed goat were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure of cauda epididymides with physiological saline (0.9% NaCl). Collected-spermatozoa wasdivided in equal volume into three tubes and diluted with Tris extender containing 20% egg yolk (control), Tris extender + 0.3 g maltose/100 ml (M0.3), and Tris extender + 0.6 g maltose/100 ml (M0.6), respectively. Dilutedspermatozoa was stored in refrigerator at 3–5oC. Quality of diluted-spermatozoa including percentages of motile spermatozoa (MS) and live spermatozoa (LS) were evaluated every day during storage at 3–5oC for four days. Data were analyzed using completely randomized design with three treatments and five replicates. Means were compared significant difference test at 0.05 significant level. Results of this study showed that mean spermatozoaconcentration, percentage of MS, percentage of LS, and percentage of abnormal spermatozoa of ettawa crossbreed goat fresh epididymal spermatozoa were 3,220 million cell/ml, 70%, 81%, and 4.3%, respectively. At day-5 of storage, percentages of MS and LS for M0.3 (38 and 60.4%) and M0.6 (38 and 57.2%) were significantly (P<0.05) higher than control (32 and 55.4%). In conclusion, addition of 0.3 and 0.6% maltose in Tris extender could be maintained viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3–5oC forthree days.
Karakterisasi Faktor-faktor Virulensi Staphylococcus aureus Asal Susu Kambing Peranakan Ettawa secara Fenotip dan Genotip Khusnan Khusnan; Wahyu Prihtiyantoro; Hartatik Hartatik; Mitra Slipranata
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.206 KB) | DOI: 10.22146/jsv.22825

Abstract

Staphylococcus aureus is a major cause of mastitis in large or small ruminants, and often manifested by subclinical mastitis in Peranakan Ettawa (PE) goats. Staphylococcus aureus in human can cause food borne disease. The research aimed to characterize the virulence factors of Staphylococcus aureus isolated from milk PE goats, phenotypic- and genotypically. Phenotypically characterization were determined through the pigmen assay as well as hydrophobicity, haemolysin, and hemaglutinin reaction. Polymerase chain reaction (PCR) analysis was used to detect 4 virulen genes including coa, clf, fnbA, and fnbB genes. The results of research showed that Staphylococcus aureus abled to produce white pigmen (35,7%), yellow pigmen g (57,1%), andorange pigmen (7,2%). Staphylococcus aureus showed α-hemolysis zone (35,7%), β-hemolysis (35,7%), dan γ-hemolysis (28,9%). Hydrophobicytic test revealed 14,3% Staphylococcus aureus isolates were hydrophobe and85,7% hydrophil. Staphylococcus aureus (85,7%) isolates abled to aglutinated sheep blood cells. Based on genotypic analysis of Staphylococcus aureus could be detected coa gene (92,8%), clf gene (64,3%), fnbA gene (78,6%), and fnbB gene (64,3%). Based on the phenotypic and genotypic characters, it can be concluded that Staphylococcus aureus are virulent strains. This information can be used as the basis for control mastitis in PE goats

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